Objective:To explore and assess the clinical application and clinical value of dual helical CT and magnetic resonance imaging (MRI) in the diagnosis of knee bone tumor. Methods: Sixty-nine patients with knee bone tumors were randomly divided into three groups: group A, group B and group C. Group A was detected by double-helical CT scanning; group B was detected by MRI; group C was detected by dual-helical CT scan combined with MRI. After the end of the testing, the clinical diagnostic results of the three detection methods were compared.Results: There was no significant difference between group A and B (x2=0.825,P>0.05). The detection rate of group C was higher than that of group A. The differences were statistically significant (x2=2.936,x2=3.718;P<0.05). The detection rate of group C was significantly higher than that of group B. The differences were statistically significant (x2=3.967,x2=4.285;P<0.05).Conclusion: Double helical CT combined with MRI can effectively improve the clinical detection rate of knee bone tumor.

Objective Studying the clinical diagnostic value of liquid-based thin-layer cytology(TCT)combined colpoacopic examination for cervical lesions.Methods In May 2006～August 2007 choose the 1407 patients coming the hospital who was done cytology examination(TCT)and coiposcopy examination and biopsy.Results Among 281 cases of eolposcopy prompt biopsy 39.50% of them suffered from CIN Ⅰ.So did 42.63 % of 699 cases of TCT examinations.427 cases of TCT combined Colposcopy were prompted they need to do biopsy.86.42% of them suffered from CIN Ⅰ or above lesions.Conclusion TCT combination of colposcopy and endoscopic biopsy can increase the rate of accurate diagnosis of cervicallesions.

OBJECTIVE To study drug-resistance status in nosocomial infection with Staphylococcus in the local region,and take effective measures to control the nosocomial infection.METHODS Totally 129 strains of Staphylococcus were clinically isolated from various specimens from Jan 2006 to Dec 2008.The strain identification,detection of meticillin-resistant Staphylococcus(MRS) and K-B disk diffusion test were performed.The data were analyzed statistically.RESULTS Among all 129 strains consisting of 6 species of Staphylococcus,the isolating rate of S.aureus predominated with 39.5%.Among them,meticillin-resistant S.aureus(MRSA) accounted for 29.4%.Among all coagulase-negative Staphyloccocus(CNS),meticillin-resistant coagulase-negative Staphylococcus(MRCNS) accounted for 32.1%.Meticillin-susceptible S.aureus(MSSA) showed significantly lower drug-resistance than MRS.CONCLUSIONS Nosocomial infection status caused by MRS is serious in the grass-roots hospitals.Effective measures should be taken to control it.

Expression levels of TGF-β1 mRNA in renal cortex of control group, diabetic group and taurine group measured by real-time quantitative RT-PCR were (7.0±0.8)×10 -3, (64.4±8.0)×10 -3 and (16.7±2.0)×10 -3, respectively. The corresponding results with end-point RT-PCR method were 0.28±0.12,0.58±0.16 and 0.43±0.10, respectively. Compared with end-point RT-PCR method, real-time quantitative with SYBR Green I was easier, faster, sensitive and specific.

Objective To study the inhibition of iron-dependent lipid peroxidation(IDLPO)by apigenin on rat cerebral homogenate in vitro and to evaluate the neuroprotective effects of apigenin on acute transient focal cerebral ischemia-reperfusion injury in rats.Methods In vitro IDLPO on rat cerebral homogenate was induced with ferrous sulfate,the incubation mixture was observed at three various concentration of apigenin and deferoxamine(DFX),and malondialdehyde(MDA)level was assayed by the 2-thiobarbituric acid(TBA)test.In vivo,the transient focal cerebral ischemia-reperfusion model in rats was established with insertion of thread embolish into middle cerebral artery.In experiment groups the neurological behavior scores,TTC stain of brain slices,and neurocyte morphology were observed.The homogenate of left hemisphere was collected for investigating the effect of apigenin on content of MDA and activity of superoxide dismutase(SOD)in 24,48,and 72 h.Results MDA was reduced in three various concentration of apigenin on rat cerebral homogenate(P0.05).In vivo,abnormal neurological behavior scores existed and typical cortical infarct lesions were found by TTC stain in both apigenin and model groups.An obvious intracellular and intercellular edema and vacuolization were found in the cerebral cortexes and hippocampuses in model group.There was karyopycnosis in glias and neurons.However lesion was alleviated in apigenin group.The MDA contents in both apigenin and model groups were increased greatly compared to ones of Sham-operated group,respectively(P

Objective To investigate the effects of penehyclidine hydrochloride (PHCD) pretreatment on expression of NF-κB and iNOS in rats with LPS-induced brain injury. Methods One hundred and five male SD rats weighing 200-220 g were randomly divided into 5 groups (n=21 each): group Ⅰ normal saline (NS);group Ⅱ LPS (L);group Ⅲ,Ⅳ,Ⅴ PHCD 0.05, 0.15, 0.45 mg/kg (D1,2,3). The animals were anesthetized with chloral hydrate 350 mg/kg. Brain injury was induced by intra-arterial LPS 150 μg administered via left internal carotid artery in group Ⅱ-Ⅴ. In group Ⅲ,Ⅳ, and Ⅴ PHCD 0.05, 0.15 and 0.45 mg/kg were given intraperitoneally (IP) at 10 min before intra-arterial LPS. The animals were decapitated at 4, 6 and 12 h after administration of PHCD (n=7 at each time point in each group). The brains were immediately removed for determination of water content, expression of NF-κB and iNOS protein and examination with light and electron microscope. Results Water content of the brain and expression of NF-κB and iNOS protein were significantly higher in group L, D1, D2 and D3 than in group NS and were significantly lower in group D2 and D3 than in group L. Intra-arterial LPS produced severe damage to the brain which was significantly attenuated by PHCD in group D2 and D3. Conclusion PHCD 0.15,0.45 mg/kg pretreatment can attenuate LPS-induced brain injury by inhibiting the up-regulation of expression of NF-κB and iNOS.

Objective To study the X-ray and CT features of costal eosinophilic granuloma for a better understanding.Methods Eight patients with costal eosinophilic granuloma proved by surgery or biopsy were analyzed retrospectively.All patients had X-ray plain film, 6 patients had CT examination,including a case of enhanced CT scan.Results All 8 lesions were solitary.Six lesions were in the anterior rib and 2 in the posterior rib.On X-ray, all case showed single cavity and oval lesion with clear boundary.On CT images, 5 lesions demonstrated expansile destruction of bone with cortical bone thinning, and 3 were osteolystic destruction with soft tissue mass around.On the patient with enhanced CT scan, the lesions showed a moderate and uniform enhancement.Conclusion The X-ray and CT findings of costal eosinophilic granuloma are characteristic.

Objective To investigate the clinical application of Cystatin C as a biological marker for monitoring hepatic pathological change in patients with virus hepatitis.Methods Two hundred and seven patients infected with hepatitis B or C virus(HBV, HCV)were divided into cirrhosis group(group A),chronic HBV group(group B),chronic HCV group(group C),and liver cancer group(group D). 32 healthy controls(group H) were recruited . The serum TIMP-1,TIMP-2,and Cystatin C as well as some traditional markers for monitoring liver function and renal function including creatinine, creatinine clearance rate, alanine transaminase, and aspartate transaminase were determined.Results In these groups, serum Cystatin C(F=28.334, P

110 g/m2 in females.④Logistic regression analysis was conducted taking sex, age, body mass index, body surface area, blood pressure, blood fat level, plasma aldosterone concentration and aldosterone synthase as independent variables while LVEDD, LVM or LVH as dependent variables respectively. RESULTS: All 68 patients were involved in the result analysis.①Among 68 cases of essential hypertension, there were 36 cases for TT genotype, 28 cases for CT genotype and 4 cases for CC genotype. And they were divided into TT genotype group and CT+CC genotypes group.②Compared with those subjects with TT genotypes, hypertensive subjects with CT+CC genotypes had a higher LVEDD, LVM and LVM index [(50.2?3.2) mm, (48.1?3.2) mm; (220.8?34.4) g, (197.4?35.5) g; (123.4?21.5) g/m2, (107.2?15.9) g/m2; t =2.73, 2.74, 3.54, P

Objective: To analyze the metabolicomics pathway in the rats with chronic glomerulonephritis intervened by tannins from Pericarpium Granati.Methods: Signal pathway analysis was carried out using the KEGG database and molecular metabolite annotation was performed using HMDB database.The enzyme or transporter and its related properties were analyzed.The metabolite path visualization was carried out by using MetPA network software.Results: The analysis of biological metabolism pathway showed that 12 metabolites involved in 16 metabolic pathways.The pathway of tryptophan metabolism, citrate cycle, phenylalanine metabolism, cysteine and methionine metabolism showed notable changes (P<0.05).Conclusion: The changes of phenylalanine metabolism, citrate cycle, tryptophan metabolism, cysteine and methionine metabolism in the rats with chronic glomerulonephritis intervened by tannins from Pericarpium Granati participate the pathological process.