The purpose of this study was to investigate the effects of different Ca~(2+) concentrations on the ventricular fibrillation threshold(VFT) in the acute reg- ional ischemic isolated, paced rat hearts and to investigate their relations to the levels of cAMP, cGMP and ATP in the myocardium. It was demonstrated that a positive correlation was observed between [Ca~(2+)] and the percentage of VFT lowered in the ischemic hearts (r=0.7998, P

Protein Z (PZ) is a vitamin K-dependent protein. As a cofactor for the protein Z-dependent protease inhibitor (ZPI), it inhibits coagulation factor X under the existence of phospholipid and calcium ion, and increases the ZPI activity by nearly 1000-fold, thus it plays a role in the process of thrombosis. ZPI inhibits coagulation factor Ⅺ a alone. ZPI activity is also consumed in the process of inhabiting factor Ⅹa and Ⅺa. This article reviews the biological characteristics of PZ and ZPI and their association with stroke.

Objective: To explore the effect of selenium-protein polysaccharide extracted from Qinba (selenium-mushroom)(秦巴硒菇) on apoptosis of K562 cells.Methods: Tumor-bearing mice model was established,and selenium-protein polysaccharide extracted from Qinba selenium-mushroom contained-herb serum was prepared by the method of serum pharmacology.K562 cells were conventionally cultured.Growth inhibition rate of tumor cells was detected by methyl thiazolyl tetrazolium(MTT) method.Morphological changes of apoptotic cell were observed under a fluorescent microscope.Cell apoptosis was observed by DNA electrophoresis.Caspase3 relative gene was measured by colorimetry.Results: Selenium-protein(polysaccharide) extracted from Qinba selenium-mushroom could significantly inhibit the growth of K562 cells,there was a remarkably positive correlation between drug concentration,time and inhibitory rate.Apoptotic phenomenon was certained via morphological examination and DNA electrophoresis.Compared with control group,caspase3 gene was markedly upregulated.Conclusion: Selenium-protein(polysaccharide) extracted from Qinba selenium-mushroom could induce apoptosis of K562 cells.The mechanism may be related to upregulation of caspase3.

OBJECTIVE: To summary the application of new types of nanoparticles carriers emerged in recent years in drug or gene delivery. METHODS: By sorting, analyzing and summarizing domestic and foreign literatures, the characteristics, in vivo and in vitro properties and the applications in pharmacy of novel nanoparticles carriers such as nano cochleates, virus-like particles, hydrogel nanoparticles, gold nanoshells, carbon nanomaterials, quantum dots and dendrimers were reviewed and elaborated. RESULTS AND CONCLUSION: Nanoparticles possess special physical and chemical properties which could improve the stability and bioavailability of drugs and have a targeting and sustained release effect.

Aged ; Endocarditis ; etiology ; therapy ; Humans ; Kidney Transplantation ; adverse effects ; Male

Pyk2(Proline-rich tyrosine kinase 2) is an important member of focal adhesion kinase family. Pyk2 is highly ex-pressed in the central nervous system and the hematopoietic sys-tem . Pyk 2 can trigger a variety of SH 2 domain-containing pro-
teins to phosphorylate their substrates, thus it can participate in the regulations including ion channel activation, stress response, cell adhesion, cytoskeleton reorganization, vesicle transport and so on. Through the regulations above, the ability of cell migra-tion, survival, proliferation changes accordingly, suggesting that Pyk2 in many important regulatory processes may become a tar-get for clinical effects. Current drug development for Pyk2 main-
ly focuses on cancer, osteoporosis and immune response. This review illustrates the domain structure, regulatory mechanisms, potential drug targets, and the drug development of Pyk2 based on the above three fields, which will provide a theoretical basis for clinical treatment.

Objective To obtain magnetic resonance imaging(MRI) symptoms that could be used in medical diagnosis and identification by analyzing the diverse MRI appearance of brain multiple sclerosis(MS).Methods MRI findings of 41 samples of clinically definite MS, including the numbers, distributions, sizes, and shapes of the lesions were analyzed. The symbolic characteristics and enhanced expression are also covered.Results Brain MS might happen singly and multiply, and more frequently with multiple happening. In a multiple case, 4—15 lesions were observed the most. Some samples showed with diffuse lesions which were unable to be counted accurately presenting as a “dirty-appearing white matter”. The distribution of the lesions was observed most frequently at the two sides of periventricle, and following by the subcortical, corpus callosum, and brainstem. Only two lesions were observed at cerebrum. The size of most lesions range from a few millimeters to 2 cm, accounting for about 75% of the total samples. Lesions with size above 2 cm were seen seldomly, with the biggest one of 6—7 cm. According to the shapes and symptoms, lesions might be categorized as an acute and chronic ones. The acute lesions had a shape of oval or circle, with a swelling appearance, low signal of T_1WI, and isointensity or a slightly higher signal on the circle. T_2WI showed a high signal, with different increase and showing “core+lunar” sign. This kind of lesions showed an enhancement with the circle enhancement as the most typical one, which had a complete circle or non-conplete arc shape enhancement, even the big lesions. The chronic lesion might also be divided into two categories. One was the quasi-symmetric lesion, with the distribution along two sides of periventricle and with the shape of small puncture/patch, part of them merged into the big patch; another kind of lesions was found scattered sparsely at frontal, parietal lobes and two sides of the periventricle, with small patches in shape. The chronic lesions had an appearance of shrinking, sharp edge, even signals, and had no lunar circle phenomena and obvious enhancement.Conclusion Brain MS have diverse MRI appearence, with some of which are unique and symbolic.

Objective:To investigate the effect and mechanism of total flavonoids of Hedysarum polybotry on induction of differentiation in human leukemia HL-60 cells. Method After the treatment of HL-60 cells with total flavonoids of Hedysarum polybotry, the cell differentiation was detected with NBT reduction method. Cell cycle, CD11b and C-fos were analysed by the flow cytometry. Result The positive rate of NBT reduction and the expression of CD11b were significantly increased. Similar, the expression of C-fos gene was upregulated. The growth of HL-60 cells was arrested at G0/G1 and G2/M phase. Conclusion Total flavonoids of Hedysarum polybotry could induce differentiation of HL-60 cells. Its molecular mechanism might be related to the modulation of gene expressions associated with the proliferation and differentiation, which leads to the inhibition of DNA synthesis.

Purpose:To assess the security and efficacy of mechanical recanalization and stenting of lilac arteries with complete occlusions without preceding thrombolytic therapy. Materials and methods:During a 3-year period,Eighteen consecutive patients underwent mechanical reeanalization and stenting for complete occlusion of the iliac artery.The method involved recanalizition with a guide wire and a catheter advanced as a while unit through the occluded segment(snowplow technique).Results The occluded segments were successfully traversed and dilated and 32 stents were placed in 18 patients.The mean ankle-brachial index (BAI)increased from 0.39?0.33 before the procedure to 0.86?0.13 after the procedure(P

Objective To evaluate the efficiency and preliminary results of transcatheter closure of perimembrane ventricular septal defects (PMVSD) using Amplartzer occluder device in children.Methods There were 5 children using transthoracic echocardiography(TTE) to confirm the PMVSD before the intervention. The diameters of the PMVSD were measured by angiography; Each of the children were treated with Amplartzer occluder device for transcatheter closure of PMVSD under TTE and fluoroscopy. The TTE and elec-trocardiograph(ECG)、chest X-ray were performed 24 hours,1 and 3、6 months after the procedure to evaluate the therapeutic effect. Results The mean diameter of the PMVSDs measured by angiography was 5.0?2.4 mm(Ranging from 2.5 to 8.3 mm). The mean diameter of the occluder selected was 7.4?3.2 mm(Ranging from 4 to 10 mm). The success rate was 100%, and no complication occurred during the procedure. No residual shunts were found by angiography immediately after the procedure in all cases. There were no malpositions of occluder and no residual shunts in the 5 cases by TTE after the procedure 24 hours , 1 and 3、 6 months. There were no cardiac arrhythmia found by ECG. It showed that both pulmonary vascularity were improved.Conclusions Transcatheter closure of perimembrane ventricular septal defects using Amplartzer occluder device is an efficient therapy for children with PMVSD. The operation is simple with a high success rate of placement and a good occlusion effect. Further studies of long term results are required.