Objective To determine whether NT 3 overexpression could abrogate DDP toxicity in vitro . Methods We constructed a herpes simplex virus(HSV) amplicon vector to transduce a c myc tagged version of rat NT 3. In the HSVnt 3myc vector, the chimeric neurotrophin cDNA was placed under the transcriptional control of the CMVIE promoter. The resultant vector was packaged utilizing the recently developed helper virus free method. Results Transduction of NT 3myc in cultured mouse cochlear explants at a multiplicity of infection(MOI) of 0 25 resulted in production of NT 3 up to 3?g/L over a 48 hours period. The mouse cochlear explants were transduced with HSVnt 3myc or HSVmiap(control vector expressing the reporter gene, murine intestinal alkaline phosphatase) for 48 hours and the exposed to cisplatin for 48 hours.The cochlear explants transduced with HSVnt 3myc had a significantly greater number of SGN survival than the control group. NT 3 powerfully enhanced the process length and the density of SGNs.Conclusion These data demonstrate that the neurotrophic cDNA transduction via HSV amplicon helper free virus system can attenuate the ototoxic action of DDP on organotypic culture. The potency of NT 3 in protecting spiral ganglion neurons from degenerating suggests that neurotrophins might be useful for the prevention or treatment of hearing disorders. [

According to the financing characteristics of military hospitals and problems in financial management; financial statement, special fund, internal control and financial indicator are used to audit economic benefits of military hospital;so that military hospitals can maximize the realization of social profits while providing good health surport.

Objective To explore the auxiliary efficacy of microbial ecological agents for elderly patients with acute pancreatitis.Methods Totally 144 elderly patients with acute pancreatitis were selected and randomly divided into the observe group and the control group (n=72,each).All patients received the regular treatment and enteral nutrition.Microbial ecological agents were added in enteral nutrition in the observe group.The levels of endotoxin (ET),tumor necrosis factor (TNF-α),interleukin (IL)-6 and C-reactive protein (CRP)in peripheral blood were determined and compared between the two groups.The clinical index and the occurrence of adverse reactions were observed.Results Compared with pretreatment,the levels of ET,TNF-α,IL-6,CRP were significantly reduced in both two groups after treatment.The levels of ET,IL-6,TNF-α,CRP were lower in the observe group than in the control group [(0.14±0.07)EU/ml vs.(0.31±0.12)EU/ml,(44.77±17.82)pg/ml vs.(63.25 ± 14.26)pg/ml,(0.15 ± 0.05)μg/L vs.(0.29 ± 0.14)μg/L,(108.33 ±11.84)mg/Lvs.(135.41±21.45)mg/L,t=14.722,43.824,7.071,5.811,respectively,allP＜0.05].The recovery time of symptoms and signs,the anal exsufflation time and hospitalization time were shorter in the observe group than in the control group [(25.8±4.8)h vs.(36.4±6.2)h,(30.9±6.7)hvs.(44.4±7.3)h,(24.9±3.2)hvs.(37.6±3.4)h,(6.9±0.4)dvs.(13.1±1.2)d,t=4.747,4.401,10.125,33.443,respectively,all P＜0.05].There were no statistically differences in death rate and the occurrence of adverse reactions between the two groups (x2 =0.15,0.64,both P＞0.05).Conclusions Microbial ecological agents are safe and effective as an adjuvant therapy for elderly patients with acute pancreatitis.It is worth promoting in clinical medicine.

Translational medicine is a new term recently which aims to eliminate the barrier between preclinical and clinical medicine,to efficiendy and effectively translate basic scientific findings into drugs and medical devices that benefits patients.This article discussed the meaning of translational medicine in cultivating postgraduates of neurology.According to the preliminary attempt,it is beneficial for postgraduates to form translational medicine philosophy and be high-quality medical talents.

Based on the review of the existing evidence of the research on the hospital management institutional ethics , this paper found researches in this area in the last two decades focused on the concept and level definition of hospital management institutional ethics;the construction of hospital management institutional ethics;the value and function of hospital management institutional ethics, and the reason and result of lack of hospital management insti-tutional ethics. On this basis, it pointed out the deficiency of the current researches and outlook the prospects.

Associating liver partition and portal vein ligation for staged hepatectomy (ALPPS) is used for patients with advanced hepatocellular carcinoma who cannot tolerate major hepatectomy due to an insufficient future liver remnant,but the morbidity and mortality rate in the perioperative period are still high.Available studies indicate that damage control surgery variations such as laparoscopic procedure and associating radiofrequency/microwave ablation/liver tourniquet and portal vein ligation could improve the morbidity and mortality associated with ALPPS,as could portal vein embolization.However,randomized controlled trials are needed to determine benefits in technical variations.

Objective To study the expression and roles of miR-31-5p in acute myeloid leukemia (AML).Methods miR-31-5p in AML patients were evaluated by real-time PCR;THP-1 cells were transfected with the miR-31-5p mimic and control, respectively.The effects of over-expression of miR-31-5p were examined by CCK-8 and FACS analysis;Dual-luciferase and Western blot were performed to detect target gene HuR expression.The effects of knock-down of HUR were also examined by CCK-8 and FACS analysis.Results miR-31-5p was down-regulated in AML patients compared to the normal control.Over-expression of miR-31-5p in THP-1 cells reduces cell proliferation and accelerates monocytic differentiation.miR-31-5p could target HuR, and knock-down of HuR inhibits cell proliferation and attenuates monocytic differentiation in THP-1 cells.Conclusions miR-31-5p may regulate AML cell proliferation and monocytic differentiation by targeting HuR.

OBJECTIVE:To observe the effects of the essential conditions of storage on quality of Semen Armenniacae Am_arum (SAA).METHODS:Gravimeteric and titration method were used and orthogonal design was adopted.RESULTS:Obvious effects of baking temperature and storage duration on content of amygdalin,temperature on acid value of SAA,temperature and humidity and storage duration on peroxide value of SAA were found.CONCLUSION:The best conditions of storing SAA are as follows:A1,B1,C3 and short storage duration.

ObjectiveTo counsel the life style from the first trimester based on the fasting hyperglysemia to decrease the rate of GDM and adverse effect of it. MethodsThe pregnancy women with fasting hyperglysemia were recruited. All subjects were divided into two groups, one was counselled the life style from the first trimester but the other not. The pregnant outcome were compared. ResultsIn the study group 1 100 with complete information and 29 were abnormal glucose intolerance, 1 120 of 47 in control. The rate of GDM or GIGT,hypertensive disease,spontaneous abortion,premature dilivery, polyhydramnios, fetal macrosomia, fetal growth restriction, neonatal respiratory distress syndrome and the mean GHbA1c was lower than that in control ( x2 = 8.110,6.124,5.815,4.518,6.355,3.957,5.453,4.619, t = 3.385, all P ＜ 0. 05 ). But the body mass index at labor( t = 0.698 ), infection disease ( x2 =0.082),mean neonatal weight (t = 2.09 )and neonatal hypoglysemia (x2 = 0.035 )of term labor had no significant differences. ( t = 0.698, χ2 = 0.082,t = 2.090, χ2 = 0.035, all P ＞ 0.05 ). ConclusionCounseling the life style from the first trimester based on the fasting hyperglysemia could decrease the rate of GDM or GIGT and improve the pregnancy result.

Moyamoya disease is a cerebrovascular disease that is characterized by the progressive stenosis in the terminal internal carotid artery, the anterior cerebral artery and the origin of the middle cerebral artery, and compensatory enlargement of basal perforating artery. Both the genetic and environmental factors involxe in its pathogenesis. but its exact cause is unknown. Revascularization is the main treatment approach for moyamoya disease. This article reviews the progress in research on epidemiology, etiology, diagnosis and treatment of moyamoya disease.