Aim To develop an HPLC method for the determination of Aconitum alkaloids extracted from Radix aconiti preparata in rats. Methods Waters 2690@996 PAD system was used. The analytical column was a Halsil 100 C18 column (250 mm×4.6 mm ID, 5 μm). The mobile phase was water, methanol and diethyl amine at the ratio of 75∶ 25∶ 0.1. The flow rate was 0.9 mL·min -1. The wavelength of the detector was 240 nm. Results The linear ranges of aconitine in the heart, spleen, lung and kidney were 0.4-100 μg·mL -1, the correlation coefficients were 0.997 2, 0.998 6, 0.999 3 and 0.999 4, respectively. The linear range of aconitine in liver was 2-200 μg·mL -1 and the correlation coefficient was 0.999 0. The linear ranges of hypaconitine in heart, liver, spleen, lung, kidney, brain and spinal cord were 5-100 μg·mL -1, the correlation coefficients were 0.999 4, 0.999 7, 0.999 8, 0.998 4, 0.999 8, 0.999 8 and 0.999 7, respectively. Detection limits (S/N=3) of aconitine and hypaconitine were 0.4 μg·mL -1. The recoveries of aconitine and hypaconitine ranged from 88.7% to 102.2% and 86.5% to 101.3%, respectively, and the RSD of precision of aconitine and hypaconitine was 10%. Conclusion It appears to be an accurate and effective method that can offer reference basis for in toxication of Radix aconiti preparata clinically.

Cone-Beam Computed Tomography ; Humans

Heart Defects, Congenital ; diagnostic imaging ; Humans ; Organ Size ; Radiography, Thoracic ; Syndrome

Objective To determine the effect of unfractionated heparin (UFH) on lipopolysaccharide (LPS)-induced expression of chemokines and nuclear factor-κB (NF-κB) signaling pathway. Methods Human pulmonary microvascular endothelial cells (HPMECs) were cultured in vitro, and the cells between passages 3 and 5 were used in the experiments. The cells were divided into control group, LPS challenge group, 1 kU/L or 10 kU/L UFH+LPS group, and NF-κB inhibitor N-tosyl-L-lysyl chloromethyl-ketone (TLCK) group (TLCK+LPS group). HPMECs in LPS challenge group were treated with 10 mg/L LPS. UFH pretreatment with different dosages groups were treated with 1 kU/L or 10 kU/L UFH 15 minutes before LPS challenge. Cells in the TLCK+LPS group were treated with 10 μmol/L of TLCK 30 minutes before the addition of LPS, and HPMECs in control group were treated with an equal volume of phosphate-buffered saline (PBS) instead. The cells were harvested 1 hour after LPS challenge, and the nuclear translocation of NF-κB was determined by immunofluorescence assay to detect the effect of UFH on NF-κB activation. The levels of interleukin-8 (IL-8) and monocyte chemoattractant protein-1 (MCP-1) in cell culture supernatants were determined by enzyme linked immunosorbent assay (ELISA) 3 hours and 6 hours after LPS challenge to detect the effect of UFH on LPS induced expression of chemokines and its mechanism of effect on NF-κB signaling pathway in HPMECs. Results ① In the control group, NF-κB was mostly located in the cytosol as shown by immunofluorescence. Treatment of HPMECs with LPS significantly increased the translocation of NF-κB from the cytosol to nucleus. UFH suppressed LPS-induced NF-κB activation both in 1 kU/L and 10 kU/L dosages, and 10 kU/L UFH gave even better results. ② Compared with control group, the levels of IL-8 and MCP-1 in the supernatants in LPS challenge group were significantly increased at 3 hours and 6 hours after LPS challenge [IL-8 (ng/L): 387.1±26.4 vs. 23.8±8.1 at 3 hours, 645.5±69.6 vs. 125.7±18.7 at 6 hours; MCP-1 (ng/L): 3 654.9±467.9 vs. 721.6±61.3 at 3 hours, 8 178.5±792.6 vs. 1 324.7±148.7 at 6 hours, all P < 0.05]. Compared with that of LPS challenge group, in 1 kU/L and 10 kU/L UFH pretreatment groups, the levels of IL-8 and MCP-1 were significantly decreased [IL-8 (ng/L): 315.3±24.8, 275.8±31.1 vs. 387.1±26.4 at 3 hours, 557.8±43.3, 496.9±38.7 vs. 645.5±69.6 at 6 hours; MCP-1 (ng/L): 2 924.1±267.9, 2 668.3±522.6 vs. 3 654.9±467.9 at 3 hours, 7 121.7±557.2, 6 563.9±576.4 vs. 8 178.5±792.6 at 6 hours, all P < 0.05]. The results indicated that 10 kU/L UFH yielded better results. However, inhibition study using the known NF-κB inhibitor TLCK could decrease LPS-induced increase in IL-8 and MCP-1 levels [IL-8 (ng/L): 162.4±21.3 vs. 387.1±26.4 at 3 hours, 274.1±22.6 vs. 645.5±69.6 at 6 hours; MCP-1 (ng/L): 1 478.2±138.5 vs. 3 654.9±467.9 at 3 hours; 3 667.6±259.4 vs. 8 178.5±792.6 at 6 hours, all P < 0.05]. Conclusions The levels of IL-8 and MCP-1 were increased obviously in LPS treated HPMECs. UFH might suppress LPS-activated NF-κB signaling pathway, contributing to the inhibitory effects of chemokines in HPMECs.

Objective To explore the different expression and clinical significance of miR-146a/133b in cervical tissue in uy-ghur and Han women in Xinjiang.Methods The relative expression of miR-146a/133b in paraffin embedding tissues of cervicitis, CIN and cervical cancer was detected by the RT-qPCR.And analyzed the clinical significance in the development of cervical cancer. Results Compared with cervicitis,the expression of miR-146a/133b increased significantly in CIN and cervical cancer(P <0.05). With the cervical lesion was aggravating,the expression level increased.In cervical cancer tissue,the expression of miR-146a were different between Uyghur and Han women(P <0.05).Marriage age<20 years old,tumor diameter≥4 cm,with HPV infection in cervical cancer tissue,miR-146a/133b had high expression (P <0.05).Conclusion MiR-146a/133b are involved in incidence and development of cervical cancer,they may become new prognostic and evaluating molecular markers in cervical cancer.

Autoimmune Diseases ; chemically induced ; Female ; Humans ; Interferons ; adverse effects ; Purpura, Thrombocytopenic, Idiopathic ; chemically induced ; Young Adult

Twenty six patients with fracture of tibial plateau was under arthroscopy assisted reduction, the joint surface of bone graft, and USES the steel plate fixation treatment. Average surgery time was 65 min (70-120 min), average fracture healing time was 15 weeks (12-17 weeks), joint surface anatomical reattachment rate was 92.9%. Using break knee function criteria evaluation of curative effect: 18 cases great 6 cases wed, 2 cases ok, fine rate was 92.3%. No infection, deep venous thrombosis and small leg fascia chamber syndrome and other complications. Conclusion is that treatment of tibial plateau fractures under arthroscope has advantages of small trauma, check intuitively and reset accurately, functional recovery of patients are satisfied, the treatment has certain clinical application value.
Adult ; Arthroscopy ; Female ; Humans ; Male ; Middle Aged ; Minimally Invasive Surgical Procedures ; Tibial Fractures ; surgery ; Treatment Outcome ; Young Adult

Objective To investigate the relationship between serum interleukin 6( IL-6 ),tumor necrosis factor α( TNF-α)and C reaction protein( CRP)levels and smoking and body mass index( BMI)in the elderly patients with stable chronic obstructive pulmonary disease( COPD ). Methods Elderly participants including 50 cases smokers with stable COPD,45 cases ex-smokers with stable COPD and 40 cases healthy ex-smokers were recruited in this study,who were hospitalized in the People's Hospital of Gansu Province from Dec. 2012 to Feb. 2014. Serum IL-6,TNF-α and CRP levels were detected. Correlation analysis was performed between serum IL-6,TNF-α,CRP levels and smoking index( SI),BMI in COPD smokers. Results The levels of serum IL-6,TNF-α and CRP in smoking group were(45. 9 ± 12. 1)mg/L,(58. 2 ± 15. 8)ng/L,(12. 2 ± 4. 1) mg/L,significantly higher than those in stop-smoking group((38. 1 ± 9. 6)mg/L,(45. 9 ± 11. 2)ng/L,(8. 6 ±3. 2)mg/L respectively),and healthy controls group((17. 0 ± 9. 9)mg/L,(27. 3 ± 13. 2)ng/L,(6. 3 ±5. 2)mg/L),and the differences were significant(F=84. 934,57. 224,23. 023;P﹤0. 01). In patients with smoking index≥400,the levels of serum IL-6,TNF-α and CRP were(50. 1 ± 12. 1)mg/L,(64. 2 ± 12. 6) ng/L,(13. 4 ± 3. 7)mg/L,significantly higher than those in patients with SI ﹤400 group((41. 0 ± 10. 2) mg/L,(47. 8 ± 14. 0)ng/L,(10. 8 ± 4. 2)mg/L respectively),and the differences were significant( t=2. 845, 4. 343,2. 347;P ﹤0. 01 or P ﹤0. 05 ). The levels of serum IL-6,TNF-α and CRP in smoking group were positively correlated with SI(r=0. 458,0. 438,0. 313;P﹤0. 01 or P﹤0. 05). The levels of serum IL-6,TNF-αwere negatively correlated with BMI,and the correlation coefficient were - 0. 358,- 0. 319( P ﹤0. 05). Conclusion The increase of serum IL-6,TNF-αand CRP caused by smoking may play an important role in the pathogenesis of COPD. And chronic smoking is the reason of the decline of BMI in COPD patients,and the decline of BMI is related to the high levels of serum IL-6 and TNF-α.

Objective To develop an HPLC method to determine the contents of chrysophanol, emodin, physcion, aloe-emodin, sennoside A, and sennoside B simultaneously in the aerial part of Rheum tanguticum Maxim.ex Balf. Methods The analysis was achieved with an Agilent ZORBAX SB-C18 analytic column (250 mm × 4.6 mm, 5 μm) by gradient elution of methanol-0.1％ phosphoric acid in water gradient elution system (0 min, 70％ B; 5 min, 65％ B;8–16 min, 60％ B; 18–23 min, 55％ B; 25–30 min, 45％ B; 32–39 min, 35％ B; 49–57 min, 24％ B; 65–72 min, 20％ B). The flow rate was 1.0 mL/min; detection wavelength was 254 nm; the column temperature was room temperature; the quantification used external standard method. Results The peak areas and concentrations of chrysophanol, emodin, physcion, aloe-emodin, sennoside A, and sennoside B showed good linear relationship within a certain concentration range (r≥0.9995); the RSD of precision was 0.75％–1.17％; the RSD of repeatability was 0.99％–2.06％; the RSD of stability was 0.97％–1.76％; the average recoveries were 99.7％–100.4％. The results showed that there were differences in content between the root and aerial part of Rheum tanguticum Maxim.ex Balf. Conclusion HPLC method for simultaneous determination of contents of 6 contents of the aerial part of Rheum tanguticum Maxim.ex Balf can be used as the references for quality control.

BACKGROUND:The inflammatory cytokines,produced by synovial cells and invaded monocytes/macrophages and lymphocytes in synovial tissues of rheumatoid arthritis(RA),play a major role in rheumatoid synovial pathology.Interleukin(IL)-17,a recently found inflammatory cytokine,associates with many autoimmune diseases.OBJECTIVE:To detect IL-17 level Levels of interleukin-17 in serum of rats with collagen-induced arthritis(CIA),to study the relationships among IL-17 and the arthritis index,IL-1?,tumor n?ecrosis factor?(TNF-?),as well as matrix meralloproteinase 3(MMP-3).DESIGN,TIME AND SETTING:A randomized controlled animal trial was performed in the Immunology Laboratory of Medical College of Dalian University from March to August 2008.MATERIALS:Fifty female,inbred Wistar rats,aged 4-6 weeks,with average weight of(180?15) g.METHODS:Forty out of 50 rats were randomly selected for establishing CIA models.The other 10 rats were regarded as the normal control group.Rats in CIA group were injected intradermally 200 ?L emulsified type-II collagen at the base of the tail after model preparation.At day 7,a second boosted injection of emulsified CII in 100 ?L was administered at the base of the tail.The rats in normal controls were injected with the same dose of saline.MAIN OUTCOME MEASURES:Paw swelling was observed at 24 hours after immunization;The levels of IL-17,IL-1?,?T?NF-? a?nd MMP-3 were examined by ELISA;The inflammatory index of CIA rats was observed.RESULTS:The levels of IL-17,IL-1?,TNF-?,as well as MMP-3 in the CIA group was greater than that of the control group(all P