1.Effects of urokinase-type plasminogen activator on chemotactic responses of spermatozoa in vitro.
Xiao-Fang DING ; Cheng-Liang XIONG
National Journal of Andrology 2005;11(6):409-418
OBJECTIVETo study the effects of the urokinase-type plasminogen activator (uPA) on the chemotactic responses of spermatozoa in vitro, and to explore the possible action mechanisms of uPA for male infertility.
METHODSThe chemotactic responses of spermatozoa were measured using spermatozoal accumulation in the capillary. According to the gradient directions of chemoattractant concentrations in the capillary, the recruits were divided into three groups: Group A (the ascending gradient of chemoattractant), Group B (the descending gradient of chemoattractant) and Group C (control). The chemoattractant in the capillary and the treating fluid in spermatozoal wells of Group A were uPA of different concentrations and Hamś F-10, respectively, while those of Group B were just opposite to Group A, and those of Group C were both Hamś F-10. Then the sperm densities in different capillaries were measured at different points of time.
RESULTS(1) Spermatozoa moved chemotactically following the concentrations of uPA. The accumulative action of spermatozoa in Group A was obviously stronger than in Groups B and C (P < 0.05 ). (2) The effects of 20 IU/ml uPA on the chemotactic responses of spermatozoa were most significant. (3) The sperm densities in the three groups increased with time, significantly different at 20 min and 30 min (P < 0.05). (4) uPA could increase sperm motility and promote sperm movement, as well as induce sperm chemotactic responses.
CONCLUSIONuPA could induce sperm chemotactic responses and increase sperm motility, which is presumably one of the action mechanisms of uPA on male infertility.
Animals ; Cells, Cultured ; Chemotaxis ; drug effects ; Dose-Response Relationship, Drug ; Male ; Rats ; Rats, Wistar ; Sperm Motility ; drug effects ; Spermatozoa ; drug effects ; physiology ; Urokinase-Type Plasminogen Activator ; pharmacology
2.Effect of butyl alcohol extract of Baitouweng Decoction on vaginal mucosal neutrophil chemotaxis in vulvovaginal candidiasis mice.
Jing-Jing JIANG ; Yun YUN ; Meng-Xiang ZHANG ; Yu YANG ; Qiang-Jun DUAN ; Tian-Ming WANG ; Jing SHAO ; Chang-Zhong WANG
China Journal of Chinese Materia Medica 2020;45(2):361-366
To investigate the effects of butyl alcohol extract of Baitouweng Decoction(BAEB) on neutrophil chemotaxis in vaginal mucosa of mice with vulvovaginal candidiasis(VVC). Seventy-two SPF female Kunming mice were randomly divided into normal control group, model group, fluconazole group, BAEB low-dose group, middle-dose group and high-dose group. Subcutaneous injection of estradiol benzoate was conducted to induce pseudo-estrus, and then 2×10~6 CFU·mL~(-1)of Candida albicans was inoculated into vaginal lumen, followed by drug treatment for 7 days. Gram staining was used to observe the morphological changes of C. albicans in vagina; vaginal fungal load was detected on agar plate. Histological changes of vaginal tissues in mice were observed by HE staining. Lactate dehydrogenase(LDH), interleukin-6(IL-6) and tumor necrosis factor(TNF-α) levels in mouse lavage fluid were detected by enzyme-linked immunosorbent assay(ELISA). Neutrophils in vaginal lavage fluid was observed and counted by using Pap smear. The levels of IL-8 and MIP-2 in vaginal mucosa were detected by ELISA. IL-8 and MIP-2 mRNA levels in vaginal mucosa of mice were detected by qRT-PCR. The results showed that as compared with the normal group, VVC model group had a large number of hyphae and a high level of fungal loadinvagina. The vaginal mucosa was completely destroyed, the number of neutrophils increased, and the protein and mRNA levels of IL-8 and MIP-2 were up-regulated. After BAEB treatment, the hyphae of the treatment group was decreased, the fungal load was decreased, the impaired mucosa showed different degrees of improvement, the inflammatory factors were decreased to varying degrees, and the protein and mRNA levels of chemokine IL-8 and MIP-2 were down-regulated. In conclusion, BAEB may be used to treat VVC by inhibiting vulvovaginal candidiasis via blocking neutrophils recruitment into vagina.
1-Butanol
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Animals
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Candida albicans
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Candidiasis, Vulvovaginal/drug therapy*
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Chemotaxis/drug effects*
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Drugs, Chinese Herbal/pharmacology*
;
Female
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Mice
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Mucous Membrane/drug effects*
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Neutrophils/drug effects*
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Vagina/diagnostic imaging*
3.Chemotactic response of ginseng endophyte to ginseng root exudates.
Xin-Xin ZHANG ; Ai-Hua ZHANG ; Feng-Jie LEI ; Li CAI ; Zhou-Yang XU ; Zhi-Qing LIU ; Lian-Xue ZHANG
China Journal of Chinese Materia Medica 2019;44(24):5358-5362
The ginseng endophytic bacteria F1 is a potential biocontrol agent for ginseng bacterial soft rot. In this paper,the chemotactic response of ginseng endophytic bacteria F1 on 8 kinds of sugar and amino acids was detected by capillary method to explore its biocontrol mechanism. The chemotactic response of F1 strain to 4 kinds of better chemotaxis substances such as glucose,glycine,L-rhamnoseand L-glutamic acid under parameters( concentration,time,temperature and pH) was studied. The results showed that under the same experimental conditions( incubation temperature 25 ℃,incubation time 60 min,chemotaxis concentration 1 mg·L~(-1)),ginseng endophytic bacteria F1 showed different degrees of response to the eight substances tested. The phenomenon of positive chemotaxis of the measured sugars and amino acids was obvious,and the chemotactic response to total ginsenosides was low. The degree of chemotaxis response is positively correlated with the chemotaxis index within a certain range of parameters,but as the temperature,p H,time,concentration and other factors continue to increase,the chemotaxis effect decreases,and F1 optimizes the chemotaxis of the four substances. The parameters are as follows: glucose: 25 ℃,10 mg·L~(-1),45 min,pH 7; glycine: 30 ℃,10 mg·L~(-1),75 min,pH7; L-rhamnose: 30 ℃,1 mg·L~(-1),30 min,pH 6; L-glutamic acid: 25 ℃,0. 1 mg·L~(-1),45 min,pH 8. The chemotactic response is more sensitive to low concentrations of chemotactic substances.
Amino Acids/pharmacology*
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Bacteria/drug effects*
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Chemotaxis
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Endophytes/physiology*
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Ginsenosides/pharmacology*
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Panax/chemistry*
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Plant Exudates/pharmacology*
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Sugars/pharmacology*
4.Effects of vasoactive intestinal peptide on chemotaxis of bronchial epithelial cells.
Cha-Xiang GUAN ; Chang-Qing ZHANG ; Xiao-Qun QIN ; Zi-Qiang LUO ; Fu-Wen ZHOU ; Xiu-Hong SUN
Acta Physiologica Sinica 2002;54(2):103-106
To investigate the influence of vasoactive intestinal peptide (VIP) on chemotaxis of bronchial epithelial cells (BECs). Rabbit chemotactic migration of primary BEC was assessed in a blind-well Boyden chamber. Radioimmunoassay and radio-ligand affinity analysis were used for determining VIP secretion and vasoactive intestinal peptide receptor (VIPR) expression. The results showed: (1) the method for determining chemotaxis of BECs by using insulin as chemotactic factor was stable and reproducible (r=0.9703, P<0.01). (2) VIP (0.001-1 micromol/L) elicited chemotaxis of BECs which was substantial and concentration-dependent. The effects of VIP were inhibited by W-7 and H-7 (P<0.01). (3) Heat stress enhanced the secretion of VIP (P<0.01) and upregulated the expression of VIPR on BECs (P<0.05). These results indicate that VIP in the lungs may play an important role in the repair of damaged epithelium, accelerating restoration of the airway to its normal state. Calmodulin and protein kinase C may be involved in the signal transduction of VIP effects.
Animals
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Bronchi
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cytology
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Cells, Cultured
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Chemotaxis
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drug effects
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physiology
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Epithelial Cells
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drug effects
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physiology
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Female
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Insulin
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pharmacology
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Male
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Rabbits
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Receptors, Vasoactive Intestinal Peptide
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biosynthesis
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Vasoactive Intestinal Peptide
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pharmacology
5.Research of chemotaxis response of Botrytis cinerea and Alternaria panax on total ginsenosides.
Kun CHI ; Yong-hua XU ; Feng-jie LEI ; Min-jing YIN ; Zhuang WANG ; Ai-hua ZHANG ; Lian-xue ZHANG
China Journal of Chinese Materia Medica 2015;40(19):3742-3747
In this paper, three kinds of chemotactic parameters (concentration, temperature and pH) were determined by plate assay and spore germination method to research the chemotactic response of Botrytis cinerea and Alternaria panax, and their spores on total ginsenosides. The results showed that Botrytis cinerea had strong chemotactic response at the mid-concentration of total ginsenosides (cultivation temperature was 20 degrees C and pH value was 6), and the data of chemotactic migration index (CMI) was 1.293 0, chemotactic growth rate (CGR) was 0.476 0, spore germination rate (SGR) was 53%, and dry weight of mycelial (DWM) was 0.452 6 g x L(-1); however, Alternaria panax had strong chemotactic response at the low-concentration of total ginsenosides (cultivation temperature was 25 degrees C and pH value was 6), and the data of chemotactic migration index (CMI) was 1.235 4, chemotactic growth rate (CGR) was 0.537 0, spore germination rate (SGR) was 67%, and dry weight of mycelial (DWM) was 0.494 8 g x L(-1). The results indicated that the low and middle concentration (2, 20 mg x L(-1)) of total ginsenosides had significant promoting effect on chemotactic response of these two pathogens, and the spore germination, mycelial growth rate, dry weight of mycelial of them were also significantly improved by this chemotactic response, whereas it decreased as the increase of total ginsenosides concentration.
Alternaria
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drug effects
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growth & development
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physiology
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Botrytis
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drug effects
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growth & development
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physiology
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Chemotaxis
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drug effects
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Drugs, Chinese Herbal
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metabolism
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pharmacology
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Ginsenosides
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metabolism
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pharmacology
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Panax
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metabolism
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microbiology
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Plant Diseases
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microbiology
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Spores, Fungal
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drug effects
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growth & development
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physiology
6.Anesthetic action of volatile anesthetics by using Paramecium as a model.
Miaomiao ZHOU ; Huimin XIA ; Younian XU ; Naixing XIN ; Jiao LIU ; Shihai ZHANG
Journal of Huazhong University of Science and Technology (Medical Sciences) 2012;32(3):410-414
Although empirically well understood in their clinical administration, volatile anesthetics are not yet well comprehended in their mechanism studies. A major conundrum emerging from these studies is that there is no validated model to assess the presumed candidate sites of the anesthetics. We undertook this study to test the hypothesis that the single-celled Paramecium could be anesthetized and served as a model organism in the study of anesthetics. We assessed the motion of Paramecium cells with Expert Vision system and the chemoresponse of Paramecium cells with T-maze assays in the presence of four different volatile anesthetics, including isoflurane, sevoflurane, enflurane and ether. Each of those volatiles was dissolved in buffers to give drug concentrations equal to 0.8, 1.0, and 1.2 EC50, respectively, in clinical practice. We could see that after application of volatile anesthetics, the swimming of the Paramecium cells was accelerated and then suppressed, or even stopped eventually, and the index of the chemoresponse of the Paramecium cells (denoted as I ( che )) was decreased. All of the above impacts were found in a concentration-dependent fashion. The biphasic effects of the clinical concentrations of volatile anesthetics on Paramecium simulated the situation of high species in anesthesia, and the inhibition of the chemoresponse also indicated anesthetized. In conclusion, the findings in our studies suggested that the single-celled Paramecium could be anesthetized with clinical concentrations of volatile anesthetics and therefore be utilized as a model organism to study the mechanisms of volatile anesthetics.
Anesthetics, Inhalation
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administration & dosage
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Biological Assay
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methods
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Cell Movement
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drug effects
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physiology
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Chemotaxis
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drug effects
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physiology
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Dose-Response Relationship, Drug
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Drug Evaluation, Preclinical
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methods
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Paramecium tetraurelia
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drug effects
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physiology
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Volatile Organic Compounds
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administration & dosage
7.Effects of BIO-1211 on eosinophil chemotaxis, recruitment and mediator release.
Xiao-yan ZHAO ; Ji-qiang CHEN ; Qiang-min XIE ; Hui-fang TANG ; Ru-lain BIAN
Journal of Zhejiang University. Medical sciences 2003;32(4):279-291
OBJECTIVETo study the effects of very late antigen(VLA) antagonist BIO-1211 on eosinophil chemotaxis, recruitment and mediator release.
METHODSEosinophil chemotaxis was induced by platelet activating factor(PAF) in vitro and eosinophil recruitment and release were determined in vivo.
RESULTVLA antagonist BIO-1211 inhibited eosinophil chemotaxis induced by PAF. The inhibitory rates at 4x10(-11), 4x10(-10), 4x10(-9) mol x L(-1) were 24.9%, 29.9%, and 31.3%, respectively. Pretreatment by BIO-1211 1, 3 and 10 mg x kg(-1) intraperitoneally inhibited the recruitment of eosinophils in PAF in the rat induced by Sephadex in a dose dependent manner. Inhibitory rates were 60.3%, 68.9%, and 72.9%(P<0.05), respectively. BIO-1211 did not inhibit eosinophil peroxidase(EPO) release from eosinophils.
CONCLUSIONBIO-1211 inhibits eosinophil chemotaxis and recruitment, alleviates local inflammation, and may represent a new type of drug for allergic diseases.
Animals ; Cell Movement ; drug effects ; Chemotaxis, Leukocyte ; drug effects ; Dose-Response Relationship, Drug ; Eosinophil Peroxidase ; Eosinophils ; drug effects ; physiology ; Integrin alpha4beta1 ; antagonists & inhibitors ; physiology ; Male ; Oligopeptides ; pharmacology ; Peroxidases ; secretion ; Platelet Activating Factor ; pharmacology ; Rats ; Rats, Sprague-Dawley
8.Effect of the Combination of Xiyanping and Cefazolin on the Function of Neutrophils in Mice.
Nanyan XIONG ; Xueling WANG ; Xiaoxia LIU ; Jianhua CHEN ; Haiping ZHENG ; Zhongchao HUO ; Junfang JIAO
Journal of Biomedical Engineering 2015;32(5):1079-1082
Xiyanping is used to treat infectious diseases with antibiotics in clinic. The aim of this study is to investigate the mechanism of Xiyanping through studying the effect of the combination of Xiyanping with Cefazolin on the chemotaxis and phagocytic function of peripheral blood neutrophils in mice. Ten healthy mice were in control group. Forty healthy mice in experimental group were infected with staphylococcus aureus, and were randomly divided further into four groups, i. e. model group, Xiyanping group, Cefazolin group and combination group (Xiyanping with Cefazolin). Mice in the control group and model group were given normal saline (NS) through abdomen while those in other groups were given Xiyanping, Cefazolin, and Xiyanping with Cefazolin, respectively. The chemotaxis of peripheral blood neutrophils was detected with the transwell method, and the phagocytic function of peripheral blood neutrophils was analyzed with flow cytometry (FCM). In the present study, there was no significance on the chemotactic index of peripheral blood neutrophils in all the groups (P > 0.05). The actual phagocytotic rate and index of peripheral blood neutrophils in the blank group, Xiyanping group, and the combination group were significantly higher than those of the model group and Cefazolin group (P < 0.05). However, those were not significant in the blank group, Xiyanping group, and the combination group (P > 0.05) or between the model group and Cefazolin group (P> 0.05). Our results suggested the combination of Xiyanping and Cefazolin could enhance the therapeutic effect by improving the phagocytic function of peripheral blood neutrophils.
Animals
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Anti-Bacterial Agents
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pharmacology
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Cefazolin
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pharmacology
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Chemotaxis
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Disease Models, Animal
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Drugs, Chinese Herbal
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pharmacology
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Mice
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Neutrophils
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cytology
;
drug effects
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Phagocytosis
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Staphylococcal Infections
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immunology
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Staphylococcus aureus
9.The effect of platelet factor 4 on the chemoattract function of the cord blood CD34+ cells.
Yi FENG ; Shi-hong LU ; Guo-li SONG ; Zhi-hua ZHANG ; Zhong-chao HAN
Chinese Journal of Applied Physiology 2003;19(2):141-144
AIMTo study the effect of PF4 and relative peptide PF4 17-70 on the chemoattract ability, the expression of adhesion molecules and CXCR4 on the flesh cord blood CD34+ cells.
METHODSCD34+ cells were separated from the cord blood using MACS immune magnetic beads, the chemoattract ability was assayed using the Transwell board, the expression of adhesion molecules and CXCR4 was measured by FACS.
RESULTS(1) PF4 and PF4 17-70 increased the migration of the CD34+ cells, the chemoattract percentage of PF4 was 157.43% +/- 50.06% (P < 0.05) and PF4 17-70 was 187.02% +/- 10.69% (P < 0.05). (2) The expression of CD49d and CXCR4 on the CD34+ cells increased after PF4 incubated, but the expressions of other adherent molecules including CD31, CD44, CD11a, CD62p, CD62E did not change.
CONCLUSIONPF4 has the chemoattract ability on the umbilical blood CD34+ cells by promoting the expression of integrin CD49d and CXCR4, PF4 may help the cord stem cells homing.
Antigens, CD34 ; metabolism ; Cell Adhesion Molecules ; metabolism ; Chemotaxis ; drug effects ; Fetal Blood ; cytology ; drug effects ; Hematopoietic Stem Cells ; cytology ; drug effects ; Humans ; Integrin alpha4 ; metabolism ; Platelet Factor 4 ; pharmacology ; Receptors, CXCR4 ; metabolism
10.The role of CD147 in the proliferation, activation and chemotaxis of Jurkat cell induced by cyclophilin A.
Fang YANG ; Xiang CHEN ; Juan SU
Chinese Journal of Hematology 2008;29(12):793-796
OBJECTIVETo investigate the role of CD147 in the proliferation, activation and chemotaxis of Jurkat cell induced by cyclophilin A (CyPA).
METHODSCD147 mRNA and protein level siRNA transfected Jurkat cells were identified by RT-PCR and Western blot respectively. Jurkat cell, Jurkat-vector cell and Jurkat-CD147 siRNA cells were treated with different concentrations of CyPA (0.01 nmol/L, 0.1 nmol/L, 1 nmol/L, 10 nmol/L) or PBS for 24 h and 48 h. Proliferation level was detected by MTT assay. CD25 was measured by flow cytometry. Transwell chamber was used to detect the chemotaxis. The effect of CyPA on the adhesive potential of Jurkat cell was studied by cell-matrix adhesion assay.
RESULTSCD147 mRNA and protein level siRNA transfected cells were decreased significantly than that of control cells. CyPA stimulated the proliferation of Jurkat cell in a dose-dependent manner, its effect peaked at 10 nmol/L CyPA. Blockage of CD147 expression decreased the proliferation level of Jurkat cell induced by CyPA. CyPA increased the activation rate of Jurkat cell, and blockage CD147 expression decreased the activation rate of the cell induced by CypA. CyPA showed a chemotactic activity on Jurkat cell, the chemotaxis index being 2.32, and the chemotactic ability was decreased after inhibition of CD147 expression. CyPA had no effect on adhesion of Jurkat cell to extracellular matrix.
CONCLUSIONCD147 plays a role in the proliferation, activation and chemotaxis of Jurkat cell induced by CyPA.
Basigin ; genetics ; Cell Adhesion ; drug effects ; Cell Proliferation ; drug effects ; Chemotaxis ; drug effects ; Cyclophilin A ; pharmacology ; Humans ; Interleukin-2 Receptor alpha Subunit ; metabolism ; Jurkat Cells ; RNA, Small Interfering ; genetics ; Transfection