No abstract available.
Calcium* ; Chemotaxis* ; Neutrophils*

The purpose of this experiment is to examine if purified Korean red ginseng extracts could have an effect on leukocyte migration and to select the best chemotactic factor for leukocyte migration. When chemotactic factor such as TC-199 medium, milk and filtrate of E.coli culture for 48hrs at 37'C extracts was used and adquet incubation periods was 60 minutes at 37'C. The various purified Korean red ginseng extract was stimulated for the leukocytes migration in modified Boyden chamber, however fraction number 2 and 4 were shown most active migration through the filter. This mean was effect an enhancement of immunological activity to leukocyte migration in vivo.
Chemotaxis* ; Leukocytes ; Milk ; Panax*

Sepsis remains one of the leading causes of death globally, in spite of advanced developments in intensive care and better understandings of pathophysiology related to sepsis. There is no special treatment or drug available for sepsis, currently. Under normal circumstances, neutrophil is a major player in acute infection control. However, during sepsis, the migration abilities and antimicrobial functions of neutrophils are impaired, resulting in a dysregulated immune response. Recent studies have indeed demonstrated that blocking or reversing neutrophil migration and impaired antibacterial function can improve the outcomes in septic animal models. This article systemically synthesized information regarding related factors and signaling involved in the functions of neutrophils in sepsis. This review also discussed the possibility that neutrophils be used as a marker for specific diagnosis and/or prediction of the outcomes of sepsis.
Animals ; Neutrophils/physiology* ; Chemotaxis ; Chemotaxis, Leukocyte ; Sepsis ; Cell Movement

No abstract available.
Antifungal Agents* ; Chemotaxis* ; Humans* ; Neutrophils* ; Phagocytosis*

No abstract available.
Asthma ; Chemotaxis ; Cytokines* ; Eosinophils* ; Interferon-gamma ; Interleukin-5

BACKGROUND: RANTES is associated with chemotaxis and activation of eosinophils. RANTES is up-regulated in allegic inflammation and play a critical role in the pathogenesis of allegic inflammation. Recently, circulating form of RANTES have been identified in the peripheral blood. METHOD: In the present study, we measured soluble RANTES levels in 17 patients with atopic brochial asthma (8 patients: early response to allegen challenge, 8 patients: early and late response to allergen challenge) on 30mins, 2hrs and 8 hrs after allergen challenge with house dust mite, prechallenge period. RESULT: RANTES levels in sera from patients with bronchial astma in prechallenge conditions were higher than in normal control subjects. But, RANTES levels in sera from patients with bronchial asthma in 30mins, 2hrs and 8hrs after challenge were no significantly higher than prechallenge conditions. CONCLUSION: These results suggest that RANTES plays a role in the pathogenesis of patients with atopic bronchial asthma and may be related to persistence of subclinical allergic inflammation.
Asthma* ; Chemokine CCL5* ; Chemotaxis ; Eosinophils ; Humans ; Inflammation ; Pyroglyphidae

OBJECTIVETo investigate the effects of C-reactive protein (CRP) on monocytes chemotaxis ability in vitro.

METHODSTranswell chemotaxis assay was used to evaluate the changes of chemotactic ability of THP-1 monocytes in each group treated with CRP in different concentration.

RESULTSCRP increased the number of attracted monocytes in response to MCP-1 (monocyte chemoattractant protein-1). When treated with CRP concentration at 2 microg x mL(-1), the number of chemotactic monocytes increased (P < 0.05). The number of attracted monocytes increased as CRP concentration was elevated (P < 0.05).

CONCLUSIONCRP can increase chemotactic ability of THP-1 monocytes in concentration dependent manner.

The present study aims to investigate the effects of the main components(aesculin, berberine hydrochloride, and anemoside B4) in the butyl alcohol extract of Baitouweng Decoction(BAEB) on the chemotaxis of neutrophils induced by dimethyl sulfoxide(DMSO). HL60 cells were cultivated in RPMI-1640 complete medium, and transferred into a 6-well plate(2 × 10~5 per mL) with 4 mL in each well, followed by incubation with DMSO at 1.3% for five days. The morphologic changes of cells were observed under an inverted microscope. The CD11 b expression after DMSO induction was analyzed by flow cytometry. The effects of aesculin, berberine hydrochloride, and anemoside B4 on the cell proliferation and migration were detected by CCK8 assay and Transwell assay, respectively. The effects of the main components on the production and polarization of F-actin protein were also examined by flow cytometry and laser confocal microscopy. PI3 K/Akt signaling pathway was checked by Western blot. As revealed by the results, neutrophil-like HL60 cells were observed after DMSO induction. The CD11 b expression in these cells increased significantly as indicated by the flow cytometry. Additionally, 100 μg·mL~(-1) aesculin, 8 μg·mL~(-1) berberine hydrochloride, and 80 μg·mL~(-1) anemoside B4 were potent in inhibiting the migration of neutrophils and reducing F-actin expression. Berberine hydrochloride was verified to be capable of diminishing phosphorylated PI3 K/Akt protein expression. The findings indicate that aesculin, anemoside B4, and especially berberine hydrochloride in the BAEB can inhibit the chemotaxis of neutrophils, which is possibly achieved by the inhibition of F-actin and PI3 K/Akt signaling pathway.
1-Butanol ; Berberine/pharmacology* ; Chemotaxis ; Drugs, Chinese Herbal/pharmacology* ; Neutrophils

Reduced chemotactic migration of polymorphonuclear neutrophil (PMN) in sepsis patients leads to decreased bacterial clearance and accelerates the progression of sepsis disease. Quantification of PMN chemotaxis in sepsis patients can help characterize the immune health of sepsis patients. Microfluidic microarrays have been widely used for cell chemotaxis analysis because of the advantages of low reagent consumption, near-physiological environment, and visualization of the migration process. Currently, the study of PMN chemotaxis using microfluidic chips is mainly limited by the cumbersome cell separation operation and low throughput of microfluidic chips. In this paper, we first designed an inertial cell sorting chip to achieve label-free separation of the two major cell types by using the basic principle that leukocytes (mainly granulocytes, lymphocytes and monocytes) and erythrocytes move to different positions of the spiral microchannel when they move in the spiral microchannel under different strength of inertial force and Dean's resistance. Subsequently, in this paper, we designed a multi-channel cell migration chip and constructed a microfluidic PMN inertial label-free sorting and chemotaxis analysis platform. The inertial cell sorting chip separates leukocyte populations and then injects them into the multi-channel cell migration chip, which can complete the chemotaxis test of PMN to chemotactic peptide (fMLP) within 15 min. The remaining cells, such as monocytes with slow motility and lymphocytes that require pre-activation with proliferative culture, do not undergo significant chemotactic migration. The test results of sepsis patients ( n=6) and healthy volunteers ( n=3) recruited in this study showed that the chemotaxis index (CI) and migration velocity ( v) of PMN from sepsis patients were significantly weaker than those from healthy volunteers. In conclusion, the microfluidic PMN inertial label-free sorting and chemotaxis analysis platform constructed in this paper can be used as a new tool for cell label-free sorting and migration studies.
Humans ; Chemotaxis ; Neutrophils/metabolism* ; Microfluidics ; Cell Movement ; Sepsis/metabolism*

Familial Mediterranean fever (FMF) is an autosomal recessive disease. Although the possibility of multiple immunologic mechanisms have been studied, the actual mechanism is still unresolved. Forty-one patients with FMF (24 males and 17 females with a mean age and disease duration of 17.8 +/- 4.1 and 4.7 +/- 2.3 years, respectively) and 14 healthy controls (10 males and 4 females with a mean age 23.2 +/- 5.1) were involved in the study. A phagotest was studied in both the patients and control groups with a FACScalibur Flow. All patients were in the acute stages of the disease and had not undergone colchicine treatment for 2 months. The percentage blood phagocytic activity of both granulocytes and monocytes were 84.23 +/- 8.76 and 67.28 +/- 10.15 in the patient group and 94.68 +/- 3.24 and 76.23 +/- 5.7 in the control group, respectively. There was no statistically significant difference in the percentage of phagocytic activity of the granulocytes and monocytes between the FMF patients and healthy controls (p > 0.05 and p > 0.05, respectively).
Adolescence ; Adult ; Chemotaxis, Leukocyte ; Familial Mediterranean Fever/immunology* ; Female ; Human ; Male ; Monocytes/immunology ; Neutrophils/immunology ; Phagocytosis*