OBJECTIVETo establish a Wistar rat model of chronic abacterial prostatitis (CAP) by injecting purified prostate protein and Freund's complete adjuvant, and to study the influence on the morphology and proinflammatory expression.

METHODSMale rats were injected with the Pertussis-Diphteria-Tetanus vaccine into the abdominal cavity and purified prostate protein and Freund's complete adjuvant intradermally at 0 and 30 days. At 60 days, the rats were sacrificed, and then the prostate specimens were observed, under the light microscope and electron microscope, and the changes of proinflammatory expression was observed too, using PCR technique.

RESULTSThe products of proinflammatory expression, such as eotaxin, iNOS and IL-4 increased markedly. The change of chronic inflammation was shown by light microscope and electron microscope.

CONCLUSIONChronic prostatitis is associated with autoimmunity.

Animals ; Autoimmune Diseases ; pathology ; Chemotactic Factors, Eosinophil ; genetics ; Disease Models, Animal ; Gene Expression ; Interleukin-4 ; blood ; Male ; Polymerase Chain Reaction ; Prostatitis ; pathology ; Random Allocation ; Rats ; Rats, Wistar

We investigated whether the production and gene expression of Gro-alpha and RANTES in Kawasaki disease differ in measles. Forty-two samples from 14 patients in different clinical stages of Kawasaki disease, eight samples from 8 patients in the acute stage of measles and seven samples from 7 healthy children were collected. The present study was performed using ELISA and RT-PCR for the productions and gene expression of the chemokines. The production of Gro-alpha was markedly elevated during the acute stage of measles compared with Kawasaki disease. Moreover, the expression of Gro-alpha was increased in every case of measles, but not in Kawasaki disease. The production of RANTES was elevated in the acute stage of both diseases when compared to the healthy control. However, the plasma RANTES level did not change significantly according to the clinical stages of Kawasaki disease. A correlation between the production and gene expression of RANTES and Gro-alpha was not found in Kawasaki disease. These results suggest that Kawasaki disease differs from measles with regard to Gro-alpha production and expression, but not RANTES. Gro-alpha might play an important role in the acute stage of measles, however not in Kawasaki disease. Further studies are needed to clarify the efficacy of Gro-alpha as a marker in measles.
Biological Markers ; Chemokines/blood/*genetics ; Chemotactic Factors/blood/*genetics ; Child ; Child, Preschool ; Comparative Study ; Female ; Gene Expression/immunology ; Human ; Infant ; Intercellular Signaling Peptides and Proteins/blood/*genetics ; Leukocytes, Mononuclear/*physiology ; Male ; Measles/*immunology/physiopathology ; Mucocutaneous Lymph Node Syndrome/*immunology/physiopathology ; RANTES/blood/*genetics ; RNA, Messenger/analysis