The measuring methods of the linearity errors and the cross contamination are very hard to grasp in testing the clinical analyzer's specifications. In this paper, the measuring steps and the data processing methods are introduced in detail in combination with the living examples and taking the form of the lists so as to help the technical personnels to understand the verification regulation of clinical analyzers correctly and to carry out the technical supervision effectively.
Chemistry Techniques, Analytical ; instrumentation ; methods ; Equipment Contamination

Calibration ; Chemistry Techniques, Analytical ; methods ; Humans ; Lead ; blood

High resolution melting (HRM), an important technology for genotyping and mutation scanning, has broad prospects in the authentification of traditional Chinese medicine. This paper selected universal trnH-psbA primers and used HRM to establish a new methods for identification of Akebia herbs. PCR was conduct at the annealing temperature of 58 degrees C and 35 cycles. The range of the DNA template concentration, the primer concentration and the Mg2+ ion concentration were further analyzed. The results showed the Tm values of Caulis Akebiae was (81.84 ± 0.16), (85.28 ± 0.16) degrees C and Caulis Clematidis Armandii was (83.22 ± 0.19) degrees C and Caulis Aristolochiae manshuriensis was (81.67 ± 0.14) degrees C, (84.24 ± 0.10) degrees C with 5-125 mg - L-' DNA template, 0.4 μmol x L(-1) primer, 2.0 mmol x L(-1) Mg2+. This method can achieve the authentification of Akebia herbs and is simple, fast, high-throughput, visual.
Chemistry Techniques, Analytical ; methods ; DNA, Plant ; chemistry ; genetics ; Genotype ; Magnoliopsida ; chemistry ; classification ; genetics ; Phylogeny ; Transition Temperature

With the constant development of the drug screening technology, new screening methods and techniques have came to the fore, driving drug screening to grow rapidly and efficiently with a high throughput. Characterized by micro-scale analysis, high throughput, inheritability and good biocompatibility, the micro-fluidic analytical technology provides a new method and technical platform for screening active ingredients from natural products. This essay introduces multiple methods used for screening active ingredients from natural products and focuses on the micro-fluidic chip screening technology combined with cell culture and its characteristics, the composition of the platform of the micro-fluidic chip screening technology and its application in screening active ingredients from natural products.
Animals ; Biological Products ; chemistry ; Drug Evaluation, Preclinical ; methods ; Humans ; Microfluidic Analytical Techniques ; methods

OBJECTIVETo compare the determination methods of fiber number concentration between China and WHO.

METHODSIndividual fiber samplings were conducted at a RCF manufacturing enterprise for 40 types of work. Flow rate was set as 2 L/min and lasted 2 to 4 hours. We used acetone-triacetin to prepare samples. The rules of two methods were used to count fibers for each sample respectively. The differences between the results of two methods were compared using the sign-rank test, and the correlation between the two methods' counting results were evaluated by the Spearsman rank correlation analysis.

RESULTSThe results of WHO counting rule were higher than those of Chinese counting rule for the same sample. The ratios of WHO method to Chinese method ranged from 1.88 to 3.70. Paired sign-rank test found the statistically significant differences of the results between the two methods (P<0.01). The rank correlation coefficient of the results by two rules counting ranged between 0.621 to 0.975, suggested positive correlation (P<0.01). The possible reasons of the difference between the two methods included the difference between the shapes of asbestos fiber and man-made mineral fiber, and counting rules of two methods.

CONCLUSIONThe results of WHO counting method is higher than those of Chinese counting method. High correlations between the results of the two methods were observed.

Asbestos ; analysis ; Chemistry Techniques, Analytical ; methods ; China ; Humans ; Mineral Fibers ; analysis ; Specimen Handling ; World Health Organization

Chemistry Techniques, Analytical ; methods ; Chromatography, Liquid ; Flow Injection Analysis ; Mass Spectrometry ; Pharmaceutical Preparations ; analysis

Animals ; Chemistry Techniques, Analytical ; methods ; Food Analysis ; Humans ; Pharmaceutical Preparations ; analysis

Separating technique of Chinese medicine is not only a key technique in the field of Chinese medicine' s research and development, but also a significant step in the modernization of Chinese medicinal preparation. Computer chemistry can build model and look for the regulations from Chinese medicine system which is full of complicated data. This paper analyzed the applicability, key technology, basic mode and common algorithm of computer chemistry applied in the separation of Chinese medicine, introduced the mathematic mode and the setting methods of Extraction kinetics, investigated several problems which based on traditional Chinese medicine membrane procession, and forecasted the application prospect.
Chemistry Techniques, Analytical ; methods ; Computers ; Drugs, Chinese Herbal ; analysis ; Medicine, Chinese Traditional ; Models, Theoretical

OBJECTIVETo observe the effects of different cell lysis buffers on protein quantification with Bradford method and bicinchoninic acid (BCA) method.

METHODSBradford method and BCA method were used to determine the concentration of bovine serum albumin (BSA) in different solutions (distilled water, cell lysis buffer used in two-dimensional differential in-gel electrophoresis and three kinds of cell lysis buffers used in conventional two dimensional gel electrophoresis), as well as the protein concentrations of cell lysates using these different lysis buffers. Bradford method was also applied to determine the protein concentrations of samples with repeated freeze thaw cycle, in different colorimetric cylinders, or using different standard curves from different periods.

RESULTThe protein measurements increased for 1.2 to 2 fold when different cell lysis buffers were used in Bradford method, but the measurements increased with the increased concentration of BSA (r=0.989 approximately 0.996, P<0.05). For BCA, measurement reading increased about thousands times higher, even overflowed the limits of machine. Protein measurements didn't change significantly, only showed a declined trend after repeated freeze thaw cycle, while no significant changes were found using different colorimetric cylinders or standard curves from different periods.

CONCLUSIONBradford method may be the choice of the protein quantification in proteomics. However, optimization is required for specific experimental conditions.

Buffers ; Cells ; Chemistry Techniques, Analytical ; methods ; Proteins ; analysis ; Serum Albumin, Bovine ; analysis ; Spectrophotometry, Ultraviolet

Biological desulfurization is a process in which sulfur compounds are removed from gas and oil using microorganisms. It is a simple process that has mild operating conditions, high desulfurization efficiency, low energy consumption and less environmental pollution. However, there is still a lack of simple and efficient analytical methods for quantitatively analyzing the sulfur compounds in the biological desulfurization process. In order to solve this problem, the analytical method for the simultaneous determination of sulfite, thiosulfate and sulfide in biological desulfurization solutions by pre-column fluorescence derivation using high performance liquid chromatography (HPLC) was developed. The standard curves of sulfur species in this analytical method had good linear relationships with correlation coefficients of 0.999 5, 0.999 7, and 0.999 7 for sulfite, thiosulfate and sulfide, respectively. The detection limits of these sulfur compounds were 0.000 6, 0.000 7 and 0.001 1 μmol/L; the range of recovery rates were 98.17 to 101.9%, 100.9 to 102.6%, and 101.1 to 104.2%; which had good repeatability and stability. The analytical method was simple, efficient and accurate, and could be used to simultaneously determine the sulfur compounds in different biological desulfurization systems.
Chemistry Techniques, Analytical/methods* ; Chromatography, High Pressure Liquid ; Sulfur Compounds/analysis*