No abstract availalbe.
Chelidonium*

OBJECTIVETo develop an HPLC method for determination of the content of chelerythrine in Chelidonium majus.

METHODChelerythrine was extracted from the fine powder of the crade with drug methanol and determined by HPLC. The mobile phase was acetonitrile-1% triethylamine (25:75) (adjusted pH to 3 using phosphoric acid) and the detection wavelength was set at 268 nm.

RESULTThe linear range of calibration curve was 0.051 6-0.516 0 microg (r = 1.000). The average recovery (n = 6) was 103.0% with RSD of 1.2%. Chelerythrine in the sample solution was stable in 8 h and the ruggedness was perfect among 3 different chromatographic columns.

CONCLUSIONThe method is accurate, sensitive and reliable.

Benzophenanthridines ; analysis ; Chelidonium ; chemistry ; Chromatography, High Pressure Liquid ; methods ; Drugs, Chinese Herbal ; analysis

In order to find new source of antifungal agents, eleven cultivable endophytic fungi were isolated from the roots,stems and leaves of Chelidonium majus by traditional method. Seven of them were identified as Colletotrichum(L1, L2, L3, S1, S3, S4, S5), and three of them were identified as Fusarium(R1,R2,R3) by morphological features and molecular biological technology. The antifungal activity test showed that all the tested fungi displayed some inhibitory activity against five common plant pathogens(C. gloeosporioides, Curvularia lunata, Pyricularia oryza, Alternaria alternate and A. brassicae), and their inhibition rate of some test items were over 60%. Among them, R1, S2, S3 and S4 were more potent than others. This study enriches the understanding of endophytes from Ch. majus and provides a basis for the study of new microbial fungicides.
Alternaria ; pathogenicity ; Antibiosis ; Ascomycota ; pathogenicity ; Chelidonium ; microbiology ; Colletotrichum ; chemistry ; isolation & purification ; Endophytes ; chemistry ; isolation & purification ; Fusarium ; chemistry ; isolation & purification

The use of traditional folk remedies is increasing throughout Asia. Chelidonium majus, a popular herbal remedy, is used to treat abdominal pain caused by various gastrointestinal disorders, including gastric ulcer, gastritis, and biliary tract disease, because of its morphine-like effect. We encountered a 62-year-old woman with acute hepatitis, in which C. majus was suspected to be the etiological factor. The patient had taken high dose of C. majus extract for the preceding 60 days. The clinical context and the temporal association between the start of the herbal medicine treatment and her liver injury allowed us to attribute a causative role to C. majus. The diagnosis was confirmed by liver biopsy and the Council for International Organizations of Medical Sciences/Roussel Uclaf Causality Assessment Method (CIOMS/RUCAM) scale. After C. majus was discontinued, the liver function was restored to normal. In conclusion, because the use of phytotherapy is increasing, we wish to raise awareness of the potential adverse effects of C. majus.
Abdominal Pain ; Asia ; Biliary Tract Diseases ; Biopsy ; Chelidonium* ; Diagnosis ; Drug-Induced Liver Injury ; Female ; Gastritis ; Hepatitis* ; Herbal Medicine ; Humans ; Liver ; Medicine, Traditional ; Middle Aged ; Phytotherapy ; Stomach Ulcer

OBJECTIVETo search for natural benzylisoquinoline alkaloids against tumor.

METHODIn this study, taken DNA as target, a method was developed for screening of natural benzylisoquinoline alkaloids against tumor from traditional Chinese medicine by the use of centrifugal ultrafiltration combined with HPLC technology. The anti-tumor activity for the compounds screened was evaluated in vitro.

RESULTNine compounds interacted with DNA were discovered and identified from Macleaya cordata, Chelidonium majus, Coptis chinensis, and the proliferation of four types of human solid cancer cell lines was markedly inhibited by these compounds.

CONCLUSIONThe developed method was considered to be suitable for screening of natural benzylisoquinoline alkaloids against tumor from traditional Chinese medicine.

Alkaloids ; analysis ; isolation & purification ; pharmacology ; Antineoplastic Agents, Phytogenic ; pharmacology ; Benzylisoquinolines ; analysis ; isolation & purification ; pharmacology ; Cell Line, Tumor ; Cell Proliferation ; drug effects ; Chelidonium ; chemistry ; Coptis ; chemistry ; Drug Evaluation, Preclinical ; Drugs, Chinese Herbal ; analysis ; isolation & purification ; pharmacology ; Humans ; Neoplasms ; drug therapy ; physiopathology ; Papaveraceae ; chemistry