BACKGROUND: Previous studies have demonstrated that Yiqihuoxue recipe (Buyanghuanwu decoction) can inhibit cell apoptosis and antagonize free radical injury in cerebral ischemia/reperfusion models. However, there have been few reports regarding the effects of Yiqihuoxue recipe on stem cell differentiation. OBJECTIVE: To observe the effects of Yiqihuoxue recipe on the differentiation of rat bone marrow mesenchymal stem cells (MSCs) towards neurons. DESIGN, TIME AND SETTING: The present controlled observational expedment based on cells was performed at the Henan University of Traditional Chinese Medicine, i.e., the Third-Grade Laboratory of the State Administration of Traditional Chinese Medicine between March 2006 and February 2008.MATERIALS: Twenty clean Sprague-Dawley (SD) rats, aged 1-2 months, weighing 100-150 g, of either gender, were included in this study. The Chinese medicine compound Yiqihuoxue recipe was made in the Manufacturing Laboratory of the First Affiliated Hospital of Henan College of Traditional Chinese Medicine as follows. 60 g Danggui (Radix Angelicae Sinensis), 30 g Taoren(Semen Persicae), 30 g Chuanxiong(Szechwan Lovage Rhizome) were selected and mixed in 720 mL water for extract volatile oil for later use. The remaining gruffs and extract were supplemented with 1 200 g Huangqi(Radix Astragali), 60 g Chishao (Radix Paeoniae Rubra), 30 g Dilong(Lumbricus), 30 g Honghua(Flos Carthami) before 920 mL water was added. After I hour of decoction and filtering, the gruffs were decocted for another 1 hour after adding 8 640 mL water. Then all decocted liquid was merged and condensed to 600 mL and mixed with 3 mL of volatile oil and tween-80. After high-temperature and high-pressure stedlization, the products were preserved for future use. 1 mL of drug was equal to 2.4 g raw herbs. METHODS: Bone marrow mononuclear cells were isolated by density gradient centrifugation. After culture, bone marrow MSCs were amplified and purified. Passage 10 cell suspension was inoculated into a 40 mm-diameter plastic culture dish. Dulbecco's modified eagle's medium supplemented with 0.1 volume fraction of fetal bovine serum and basic fibroblast growth factors was added for 24 hours of culture when adherent cells reached 60%-90% confluence in each group. Thereafter, the "Yiqihuoxue" group was incubated for 5 hours using medium containing DMSO, butylated hydroxyanisole, 13-mercaptoethanol, and Yiqihuoxue recipe; simultaneously, the control group was treated for 5 hours with fluid containing DMSO, butylated hydroxyanisole, and β- mercaptoethanol.MAIN OUTCOME MEASURES: Identification of bone marrow MSCs by flow cytometry; Detection of Nestin-, and neuron specific enolase(NSE)-, and glial fibrillary acidic protein(GFAP)-positive expression by immunohistochemistry. RESULTS: Flow cytometery results demonstrated that cell surface marker CD90 and CD106 expression was positive, while CD45 and CD34 expression was negative. Immunohistochemistry results showed that the numbers of Nestin-positive cells (1 and 3 hours after induction) and NSE- and GFAP-positive cells (5 hours after induction) were significantly greater in the Yiqihuoxue group than in the control group (P < 0.01). CONCLUSION: Yiqihuoxue recipe can induce the differentiation of bone marrow MSCs towards neurons in vitro.

Moraxella Catarrhalis emerged as the third cause of respiratory tract infection in children.Over 90% of the Moraxella Catarrhalis strains isolated currently produced by β-lactamases positive.Moraxella Catarrhalis resist to Ampicillin because of the β-lactamases,such as the BRO-1 type,BRO-2 type and BRO-3 type.The BRO genes appeared to be located on the chromosome and be coded.Twenty-one new mutations were found in the putative promoter region of the BRO genes.

Thirty patients with vitiligo were treated by cupping of skin lesion and compress of Chinese herbs, compared with western medical treatment. After 3-course's treatments the total effective rate ws 96.7% in treatment group and 76.7% in control group. There was a significant difference (P＜0.05)between the two groups shown by statistical analysis.

The progress in the experimental and epidemiological studies on the mutagenic and carcinogenic effects of gasoline engine exhausts were reviewed in the present paper. Current data showe that gasoline engine exhaust is a genotoxic agent and mutagen to bacteria, animal and human, but for its carcinogenic effects on animal by inhalation route, no new report has been published. So far no consistent relationship has been seen between cancer risk and gasoline engine exhaust exposure in epidemiological fields.

Objective To investigate the correlation between blood pressure variability and variabilities of some feature variables extracted from photoplethysmography (PPG) signal.Methods Continuous blood pressure (BP) and PPG signal were collected simultaneously from 19 healthy subjects under rest and after exercise.Eight feature variables were extracted from the PPG signal.The relationship between the beat-to-beat variability of BP and feature variables extracted from PPG were quantified by calculating Person's correlation coefficient.Results The variability of the amplitude of PPG was highly correlated with systolic BP variability both under rest (0.75) and after exercise (0.76).Meanwhile,the correlations between systolic BP variability and the variability of the time from the trough to the dicrotic north were 0.74(under rest) and 0.78(after exercise).Conclusion The amplitude variability of PPG is highly correlated with systolic BP variability and can be potentially used for monitoring BP variability.

Objective:To analyze the effects of super early rehabilitation nursing on poststroke depression, to sum up the nursing experience.Methods: The control group included 78 cases of stroke patients, the observation group included 77 cases, the observation group was given super early rehabilitation nursing after stroke seventh days, thirtieth days, ninetieth days, with Hamilton Anxiety Scale(HAMA) and Depression Scale(HAMD)assessment of patients with anxiety or depression levels, and statistical correlation index.Results: Stroke after 7 days, 30 days and 90 days in PSD group, severe occurrence of PSD was higher than that in the observation group, stroke after 30 days, 90 days in the control group are more likely to commit suicide than the observation group, stroke after 90 days of control group, PSD turnover rate is higher than that of the control group, the difference was statistically significant specific(x2=6.62,x2=13.74;P<0.05).Conclusion: PSD can occur in each time period after stroke, most self outcome; super early rehabilitation nursing is helpful to reduce the incidence of PSD risk.

Phosphatidylserine (PS) is a phospholipid that is abundant in eukaryotic plasma membranes,has crucial biological functions.Under cell apoptosis, cells can not generate enough ATP for energy and the concentration of cytoplasmic Ca 2 +increases, resulting in PS eversion.Apoptosis and the clearance of apoptotic cells are essential processes in animal development and homeostasis.For apoptotic cells to be cleared, they must display aneat me signal, most likely PS exposure, which prompts phagocytes to engulf the cells.PS is exposed by the action of scramblase on the cell's surface in biological processes such as apoptosis and platelet activation.Once exposed to the cell surface, PS acts as an eat me signal on dead cells, and creates a scaffold for blood-clotting factors on activated platelets.The molecular identities of the flippase and scramblase that work at plasma membranes have long eluded researchers.Indeed, their identity as well as the mechanism of the PS exposure to the cell surface has only recently been revealed.We describe how PS is exposed in activated platelets and in apoptotic cells, and discuss the clearance of apoptotic cells.

In recent years, with the rapid development of antitumor targeted drug therapy, the preparation of antitumor liposomes and its targeting research have attracted attention of scholars. Anti-tumor targeted drug treatment requires the drug to reach the tumor site has a relatively high concentration and longer retention time, the tumor cells have a strong cytotoxic activity, while normal cells no significant side effects.The drug has an in vivo distribution and specificity for the target fine action.The preparation methods of liposomes include film dispersion method,reverse evaporation method,ethanol injection method,supercritical reverse phase evaporation method and freeze-drying method. Antitumor targeting liposomes can be divided into active targeting liposomes and passive targeting liposomes. Targeted liposomes can specifically target tumor cells by recognizing specific targets in the tumor tissue,thus enriching tumor cells and killing tumor cells. In this paper,the preparation of anti-tumor liposomes and the progress of its targeting,for future study and application of liposomes provide a reference.

To adapt to the new medical enviroment and aiming at the problems existing in clinical teaching,the author explores the value and feasibility of new cultivation mode and its concrete methods to make clininal educatinon play more inportont role in medical education and medical elite cultivation

Objective:To evaluate the ectopia osteogenesis ability of rhBMP-2/nHA/Co compound membrane.Methods:Two membranes were fabricated:(1) nHA/Co group(2) rhBMP-2/nHA/Co group.Both of them was evaluated after implantation under thigh muscle of nude mice for 10,20,30 days.Results:More bone-like structures in rhBMP-2/nHA/Co group were found,than that in nHA/Co group.Conclusion:Collagen and nano-HA are suitable sustained release systems for rhBMP-2 in animal experiment and the rhBMP-2/nHA/Co compound membrane can effectively induce ectopia osteogenesis.