OBJECTIVES: We aimed to evaluate the effectiveness of Er:Cr:YSGG laser therapy on the reduction of dentinal hypersensitivity while taking into account the length and depth of the cervical abrasion. METHODS: We included adults (age, 20-60 years) with at least 2 hypersensitive teeth. The hypersensitive teeth were stimulated with a pressure-indicating probe and an air syringe. The response of the subjects to this stimulation was quantified using a visual analog scale (VAS) and Schiff air sensitivity score. The patients were treated at baseline, immediately after laser treatment, and at 1, 2, and 4 weeks after the Er:Cr:YSGG laser therapy. The results were analyzed by repeated measure analysis of variance (ANOVA) and Bonferroni's post hoc test. RESULTS: The values of tactile test and air blast test showed statistically significant differences between the following time points: baseline and immediately after laser treatment, baseline and first week after treatment, and immediately after laser treatment and first week after treatment (P<0.05). No statistically significant differences were observed in the VAS values of the tactile test and Schiff air sensitivity score of the air blast test between the first and second, first and fourth, and second and fourth weeks after treatment (P>0.05). The depth and width of the cervical abrasion was not statistically significant in dentinal hypersensitivity reduction (P>0.05). CONCLUSIONS: The use of Er:Cr:YSGG laser therapy reduced dentinal hypersensitivity, especially immediately after laser treatment. The depth and width of the cervical abrasion is not statistically significant in dentinal hypersensitivity reduction.
Adult ; Dentin ; Humans ; Hypersensitivity ; Laser Therapy ; Syringes ; Tooth

Background and Objectives: The colonic epithelial layer is a complex structure consisting of multiple cell types that regulate various aspects of colonic physiology, yet the mechanisms underlying epithelial cell differentiation during development remain unclear. Organoids have emerged as a promising model for investigating organogenesis, but achieving organ-like cell configurations within colonic organoids is challenging. Here, we investigated the biological significance of peripheral neurons in the formation of colonic organoids. Methods: and Results: Colonic organoids were co-cultured with human embryonic stem cell (hESC)-derived peripheralneurons, resulting in the morphological maturation of columnar epithelial cells, as well as the presence of enterochromaffin cells. Substance P released from immature peripheral neurons played a critical role in the development of colonic epithelial cells. These findings highlight the vital role of inter-organ interactions in organoid development and provide insights into colonic epithelial cell differentiation mechanisms. Conclusions Our results suggest that the peripheral nervous system may have a significant role in the development ofcolonic epithelial cells, which could have important implications for future studies of organogenesis and disease modeling.

During mid-life, women experienced not only physical but also neurological transition. Because of this, many women suffer from physiological and/or psychological menopausal symptoms. Although hormone therapy (HT) was broadly used to alleviate menopausal symptoms, HT showed inconsistent effects in case of psychological symptoms. Moreover, mid-life women’s brains have distinct characteristics than in other periods of life, it is needed to study psychological symptoms in shifted brain network of mid-life women. As an alternative, inhalation of fragrances may alleviate psychological menopausal symptoms. To characterize the alleviation mechanism by fragrances, we tested the effect of fragrances on menopausal symptoms using electroencephalographic (EEG) methods. We hypothesized that fragrance could restore mid-life women’s brain response to stressful situations. We tested six fragrance conditions, including no-odor condition (solvent only) in twenty-eight mid-life women (49.75 years±3.49).Our results showed that fragrances increased alpha power and decreased β/α ratio depending on the severity of menopausal symptoms in a stressful situation. Our study would be helpful in psychological menopausal symptom alleviation as well as fragrance screening for well-being in mid-life.