1.Investigation on relationship between whole blood zinc and Fe elements with children pneumonia caused by respiratory syncytial virus
International Journal of Laboratory Medicine 2016;37(17):2401-2402,2405
Objective To investigate the relationship between zinc and Fe microelements with the respiratory syncytial virus (RSV) infection and to find a clinical and practical treatment and prevention method for RSV caused pneumonia .Methods Sixty children patients with RSV pneumonia were enrolled in the observation group ,and contemporaneous sixty healthy children served as the control group .Then the concentrations of whole blood zinc and Fe microelements were detected by using atomic absorption spectroscopy .Results Compared with the healthy control group ,the levels of whole blood zinc and Fe microelements in different age periods of RSV pneumonia group were significantly decreased with statistical difference(P<0 .05) .Conclusion The decrease of whole blood zinc and Fe microelements levels in children may be associated with RSV caused pneumonia .
2.Establishment of residual ridge resorption model of rats after the mandible incisor was extracted
Zhe WU ; Hongchen SUN ; Yanhai CHE
Journal of Practical Stomatology 2001;0(01):-
Objective:To establish an experimental model of residual ridge resorption after mandibular incisor extraction and to study the occurrence mechanisms.Methods:Thirty male Wistar rats were used, the edge of the right mandibular incisor was cut every three days(a total of three times) and the incisor was extracted at three days after the final cut. The animals were sacrificed 0,1,2,4,8,12 weeks respectively after the extraction and the mandibles were dissected out. The length of the alveolar bone and the ratio of extracted side length to unextracted side length were measured on soft X-ray photographs and the mandibles were processed for histomorphometry.Eighteen male Wistar rats were used to observe the morphologic alteration of periodontal tissues by hematoxylin and eosin when the incisor crown was cut at different time intervals.Results:As compared with the unextracted side, the length of alveolar ridge of the extracted side was significantly decreased at 4,8,12 weeks;the ratio of extracted side length to unextracted side length significantly decreased at 4 week after tooth extraction(P
3.Anticoagulant effects of Pheretima extracts
Hong HE ; Qingming CHE ; Qishi SUN
Chinese Traditional and Herbal Drugs 1994;0(05):-
Objective To study the anticoagulant effects of Pheretima extracts and analyze the effective constituents. Methods Antithrombotic effects of Pheretima extracts were observed by determining the coagulation time, bleeding time of mice and the recalcification time of rabbit plasma in vitro, meanwhile the content of free amino acid in the extracts were determined. Results Pheretima extracts could prolong the coagulation time, bleeding time of mice, and the recalcification time of rabbit plasma in vitro. The content of free amino acid was rich in Pheretima extracts. Conclusion Free amino acid in Pheretima extracts may be the main anticoagulant effective constituents.
4.Heterologous expression and substrate specificity of ketoreductase domain in bacillaene polyketide synthase.
Xiaohui SUN ; Chengchuan CHE ; Junjie JI ; Jianting ZHENG ; Ge YANG
Chinese Journal of Biotechnology 2015;31(9):1355-1362
The ketoreductase (KR) domain in the first extending module of the polyketide synthase (PKS) catalyzes the reductions of both an α-keto group and a β-keto group in the biosynthesis of bacillaene, suggesting the intrinsic substrate promiscuity. In order to further investigate the substrate specificity, the KR domain (BacKR1) was heterologously overexpressed in Escherichia coli. In vitro enzymatic analysis showed that only one of the four diastereomers was formed in the reduction of the racemic (±)-2-methyl-3-oxopentanoyl-N-acetylcysteamine thioester catalyzed by BacKR1. In addition, BacKR1 was revealed to catalyze the reductions of cyclohexanone and p-chloroacetophenone, indicating the potential of KR domians of PKSs as biocatalysts.
Bacterial Proteins
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genetics
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metabolism
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Catalysis
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Cyclohexanones
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metabolism
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Escherichia coli
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enzymology
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Polyketide Synthases
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genetics
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metabolism
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Protein Structure, Tertiary
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Substrate Specificity
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omega-Chloroacetophenone
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metabolism
5.Study on optimizing human acellular dermal matrix and fluorescence labeling the Co-cultured fibroblasts
Hong SUN ; Mengcun QI ; Yuqiang ZHANG ; Jianzhong CUI ; Pengcheng CHE
Acta Anatomica Sinica 2009;40(4):609-613
Objective To optimize human acellular dermal matrix(ADM) and evaluate its biological characters. Methods Human skin was treated with hypertonic saline followed by NaOH maceration(group A), hypertonic saline followed by sodium dodecyl sulfate (SDS) detergent(group B) or Dispase Ⅱ followed by Triton X-100(group C), the resulting ADM were sectioned, and then were stained by special immunohistochemistry method. The cytotoxicity of them were evaluated by methyl thiazolyl tetrazolium (MTT) colorimetry and then cell compatibility was analyzed by cell culture;The optimized ADM resulted was choosen for use. Fibrablasts(FBs)were transfected with adenovirus vector encoding green fluorescent protein gene(Ad-GFP)and the growth of them on the optimized ADM was observed by fluorescent microscopy. Results Collagen and elastic fibers can still be observed in three kinds of ADM. The cells in dermis can be disintegrated both in group A and C, but not in group B. The cytotoxicity scores of the ADM prepared in group A and B were grade 0 or grade 1, while that of group C was more than grade 1.The ADM prepared by NaCl-NaOH maceration had good biocompatibility. There was statistical difference in adhering number of NIH3T3 cells in group A and B. NIH3T3 cells grew well in group A and the resulted ADM was optimized. FBs transfected with Ad-GFP grew well in the optimized ADM. Conclusion The ADM prepared by NaCl-NaOH maceration was a good tissue engineering biomaterial with a little cytotoxicity and rich in resouce.
6.Analysis on prognostic related factors of surgical treatment in patients with ruptured intracranial aneurysm and their clinical significance
Jiujun SUN ; Zhaohui HE ; Jiuning TANG ; Xudong CHE
Chongqing Medicine 2014;(36):4872-4874
Objective To investigate the prognostic related factors in the patients with ruptured intracranial aneurysm treated by surgical treatment and their clinical significance .Methods The medical record data in 218 cases of ruptured aneurysm treated by operative treatment were performed the retrospective analysis ,including th egeneral data ,Hunt‐Hess grade on admission ,surgical procedure ,etc .The patient′s prognosis situation adopted the Glasgow outcome scale(GOS) as the evaluation standard when dis‐charge from hospital .Firstly the possible factors were performed the single factor analysis ,then the factors with statistic signifi‐cance in the single factor analysis were performed the multiple factors logistic regression analysis .Results The single factor analy‐sis indicated that the age ,Hunt‐Hess grade on admission ,hypertension ,operation mode ,CT Fisher grading and symptomatic vaso‐spasm were significantly correlated with the prognosis ( P < 0 .05) .The multiple factors analysis indicated that age ,Hunt‐Hess grade on admission ,symptomatic vasospasm ,CT Fisher grading were the independent risk factors affecting the prognosis of patients (P< 0 .05) .Conclusion The patients′ age ,Hunt‐Hess grade on admission ,symptomatic vasospasm and CT Fisher grading can sig‐nificantly affect the prognosis of the patients with ruptured intracranial aneurysm after surgical treatment .Paying attention to the predictive value of these predictors has important clinical significance to reduce the postoperative mortality rate and disability rate in the patients with ruptured intracranial aneurysm .
7.Effect of labor analgesia on development of postpartum depression
Yue SU ; Jie SUN ; Mingjun XU ; Xiangming CHE
Chinese Journal of Anesthesiology 2015;35(3):317-319
Objective To evaluate the effect of labor analgesia on the development of postpartum depression.Methods Seventy nulliparous parturients who were at full term with a singleton fetus in vertex presentation,aged 20-35 yr,with body mass index<27 kg/m2,at 38-41 weeks of gestation,of ASA physical status Ⅰ,were enrolled and divided into 2 groups (n =35 each) using a random number table:vaginal delivery group (group VD) and labor analgesia group (group LA).In LA group,the epidural catheter was placed at L2,3 interspace for combined spinal-epidural analgesia when their cervical dilations were in 2-3 cm.VAS score was maintained below 3 after the analgesia.Parturients completed Edinburgh Postnatal Depression Scale questionnaires 42 days after the labor.The development of depression was recorded.Results The incidence of postnatal depression was significantly lower in LA group (17%) than in VD group (40%).Conclusion Labor analgesia can decrease the development of postpartum depression.
8.Observations on the Efficacy of Electroacupuncture plus a Multi-function Cervical Rehabilitation System in Treating Cervical Spondylosis
Zheng HUANG ; Qiping DAI ; Jian SUN ; Yunpeng WU ; Tao CHE
Shanghai Journal of Acupuncture and Moxibustion 2015;(11):1088-1090
Objective To investigate the clinical efficacy of electroacupuncture plus a multi-function cervical rehabilitation system in treating cervical spondylosis.Methods Sixty patients with cervical spondylosis were randomly allocated to treatment and control groups, 30 cases each. The treatment group received electroacupuncture plus treatment with a multi-function cervical rehabilitation system and the control group, electroacupuncture alone. After 30 days of treatment, changes in the pain score, the neck disability index, and cervical active range of motion and isometric muscle strength were observed in the two groups and the clinical therapeutic effects were compared between the two groups.Results The total efficacy rate was 96.7% in the treatment group and 93.3% in the control group; there was no statistically significant difference between the two groups (P>0.05). There were statistically significant pre-/post-treatment differences in the pain score, the neck disability index, and cervical active range of motion and isometric muscle strength in the two groups (P<0.01,P<0.05). There were statistically significant post-treatment differences in the neck disability index, and cervical active range of motion and isometric muscle strength between the treatment and control groups (P<0.05).Conclusions Electroacupuncture plus a multi-function cervical rehabilitation system is an effective way to treat cervical spondylosis. It can relieve the clinical symptoms in the patients.
9.Electromagnetic Compatibility of Medical Devices on Health-Care Train
Yu CHE ; Hanjun SUN ; Dan HUANG ; Changning SHI ; Lei ZHU
Chinese Medical Equipment Journal 2004;0(09):-
The problems of Electromagnetic Compatibility(EMC) and Electromagnetic Interference(EMI) of medical electric devices are directly related to the medical treatment effect for wounded patients on the health-care train.EMC and EMI problems of medical electric devices in use are studied and analyzed.Suggestions on preventing EMI problems are given to guarantee the normal running of medical electric devices in health-care train.
10.The expression of GST-π protein in peripheral blood and brain of patients with drug-resistant epilepsy and refractory epilepsy rats
Zhiqing SUN ; Qingxi FU ; Lixiang TU ; Fengyuan CHE ; Quanping SU
Chinese Journal of Nervous and Mental Diseases 2016;42(2):80-84
Objective To investigate the expression of glutathione S-transferase π (Glutathione S-transferase π, GST-π) protein in peripheral blood and brain of patients with drug-resistant epilepsy and refractory epilepsy rats. Meth?ods From January 2010 to March 2014, the expression of GST-πin the blood and brain of 32 cases of drug-resistant epi?lepsy underwent neurosurgery and 10 cases of cerebral vascular malformation underwent surgery were studied and com?pared. The expression of GST-πin the blood and brain in refractory epilepsy rats and normal rats were studied and com?pared. Results The specimen from 20 temporal, 6 frontal and 6 occipital lobes were obtained from drug-resistant epilep?sy patients. The expression levels of GST-πin the blood and brain in refractory epilepsy rats and normal rats were higher than those of the control groups (P<0.05). Conclusion GST-πmay be involved in the process of drug-resistant epilepsy. The GST-πexpression in blood may be used as a marker for resistance to anti-epileptic agents.