Long non-coding RNAs are important regulators of gene expression.ANRIL which was coded on the Chr9p21.3 loci participates in the pathogenesis of tumor, coronary artery disease, type 2 diabetes mellitus and oth-er diseases.Multiple ANRIL isoforms are tissue-specific.ANRIL mainly functions through Polycomb proteins, while there are also other downstream targets.The mechanism of each isoform and the downstream pathways are hotspots incurrent researches.

AIM: To study effects of tumour necrosis factor alpha(TNF?) and interleukin-10(IL-10) on human cytomegalovirus AD169 (HCMV AD169) infection in human embryonic lung fibroblasts (HEL),and the ability of the infected HEL to produce TNF?. We have attempted to understand the effect of cytokines in the immune of HCMV infection. METHODS: TNF??IL-10 were added separately with different concentrations before 24 h HCMV infection of HEL to study the effect of TNF? and IL-10 on multiplication of HCMV. HCMV was incubated with HEL, TNF? in culture supernatants were measured at 4, 24, 48, 72, 96 h postinfection. The level of TNF? was measured by enzyme-linked immunosorbent assay( ELISA). RESULTS: The addition of TNF? with the concentration of 10-100 ?g/L or IL-10 with the concentration of 1-10 ?g/L before 24 h HCMV infection of HEL could remarkably inhibited the multiplication of HCMV in HEL. Level of TNF? didn't increase in infected cell supernatants. CONCLUSION: TNF? and IL-10 play an important role in the immune of HCMV infection.

Objective To compare pituitrin resuscitation,hypertonic saline resuscitation versus hydroxyethyl starch (HES) resuscitation during the early stage in a dog model of severe uncontrolled hemorrhagic shock (UHS).Methods Adult Chinese rural dogs of both sexes,weighing 10-12 kg,underwent sever UHS by transecting one branch of mesenteric arteries,followed by blood withdrawal via the femoral artery to target mean arterial pressure (MAP) of 50 mm Hg.Twenty-four dogs with severe UHS were randomized into 3 groups according to resuscitation strategies (n =8 each):pituitrin resuscitation group (group P),hypertonic saline resuscitation group (group SA),and resuscitation with HES (group HES).In group P,pituitrin was infused at a rate of 0.04U· kg-1 · min-1 after a loading dose of 0.1 U was given intermittently.A single bolus of 7.5 ％ hypertonic saline 6 ml/kg was injected in group SA.HES 200/0.5 was infused at a rate of 18-38 ml· kg-1· h-1 in group HES.MAP was maintained no lower than 50 mm Hg in each group.The branch of mesenteric arteries was ligated 1 h after resuscitation and all the blood initially shed was returned in each group.The parameters of hemodynamics were recorded before UHS (T0),after successful UHS (T1),at 15,30,45 and 60 min of resuscitation (T2-5),and at 2 h after hemostasis and return of shed blood (T6).Arterial blood samples were taken at T0,T1,T5 and T6 for blood gas analysis.Venous blood samples were collected at T0,T5,T6 and 3 days after extubation for determination of serum TNF-α,IL-10 and adrenocorticotropic hormone (ACTH) concentrations.TNF-α/IL-10 ratio was calculated.The survival rate was measured within 72 h after hemostasis and return of shed blood.The volume of blood loss was recorded during UHS phase and uncontrolled bleeding resuscitation phase.Results Compared with group P,SBP,DBP,CVP,HR,serum TNF-α concentration and TNF-α/IL-10 ratio were significantly increased,and Hct and serum IL-10 concentration were decreased in group SA,and SBP,HR,Lac,TNF-α and ACTH concentrations,and TNF-α/IL-10 ratio were significantly increased,and serum IL-10 concentration was decreased in group HES (P ＜ 0.05).SBP,DBP,CVP,HR and serum IL-10 concentration were significantly lower,and Lac,TNF-α and ACTH concentrations,and TNF-α/IL-10 ratio were higher in group HES than in group SA (P ＜ 0.05 or 0.01).The volume of blood loss recorded during uncontrolled bleeding resuscitation phase was significantly larger in group SA than in P and HES groups (P ＜ 0.05).There was no significant difference between P and HES groups in the volume of blood loss recorded during uncontrolled bleeding resuscitation phase (P ＞ 0.05).There was no significant difference in the survival rate between the three groups (P ＞ 0.05).Conclusion Resuscitation with continuous infusion of a small dose of pituitrin can maintain the blood pressure stable with less blood loss and inhibit stress responses and inflammatory responses,and the efficacy is superior to that of resuscitation with a small dose of hypertonic saline or HES resuscitation.

To compare the diagnostic value of high-resolution uhrasonography (HR-US) with nerve conduction study (NCS) in patients with clinically defined carpal tunnel syndrome (CTS),a prospective study was conducted on 37 consecutive patients investigated for sensory hand symptoms. With the clinical diagnosis of CTS as gold standard,NCS showed higher diagnostic sensitivity (80％)than ultrasound (61％) (P =0.047 ).The positive predictive value of HR-US for CTS was 100％.The results indicated that HR-US could be used as a screening method for majority of clinically suspected CTS patients and only for those with negative HR-US results.

Objective:To explore the effect of rehabilitation nursing on muscle spasm of patients in persistent vegetative state (PVS). Methods:To adopt the rehabilitation nursing methods on 98 PVS patients, including the arrangement of comfortable body position, passive motion, massage, etc.and then to evaluate the patients’ spasm situation with the universally emendatory MAS (motor assessment scale) methods. Results:Under the rehabilitation nursing and correlative curing, the patients’ limbs muscular tension had been improved evidently(x2=17.04, x2=14.04, P<0.01). Conclusion:Good limb position and physical movement rehabilitation nursing training do not need special equipments or techniques. If only the nursing personnel could master the training methods and skills, they could help the PVS patients to control and to decrease the muscle spasm and then to help them ease the pain.

Objective Compared with povidone iodine solution to clean,glutaraldehyde immersion,highpressure steam sterilization of three disinfection methods for dental handpiece sterilization effect.Methods Select the 20 dental clinical used over phone,were randomly divided into A group,B group,C group,D group 5,A group for the control group,only cleaning method did not use any disinfection,B group was 1% Polyvinylpyrrolidone iodine solution,wipe,C group were soaked in 2% glutaraldehyde soluton,D group were treated with high-pressure steam sterilization,after each phone were inoculated with bacteria sample dish,the more monitoring of four groups of bacterial culture.Results A group of bacterial culture for the intensive growth of bacteria,B group had bacterial growth for the(+ ～+ + +),bacterial growth;C group had bacterial culturegrowth was(+ ～ + +),bacterial growth;D group of bacterial culture Growth of(-),no bacterial growth.Conclusion High-pressure steam sterilization was the disinfection of dental handpieces most effective way.

The molecular modifications of Herpes Simplex Virus Type Ⅰ (HSV-1) proteins represented by acetylation and phosphorylation are essential to its biological functions.The cellular chromatin-remodeling/assembly is involved in HSV-1 associated gene transcriptional regulation in human cells harboring HSV-1 lytic or latent infections.Further investigation on these biological events would provide a better understanding of the mechanisms of HSV- 1 viral gene transcriptional regulation.

Objective:To analyze the effects of super early rehabilitation nursing on poststroke depression, to sum up the nursing experience.Methods: The control group included 78 cases of stroke patients, the observation group included 77 cases, the observation group was given super early rehabilitation nursing after stroke seventh days, thirtieth days, ninetieth days, with Hamilton Anxiety Scale(HAMA) and Depression Scale(HAMD)assessment of patients with anxiety or depression levels, and statistical correlation index.Results: Stroke after 7 days, 30 days and 90 days in PSD group, severe occurrence of PSD was higher than that in the observation group, stroke after 30 days, 90 days in the control group are more likely to commit suicide than the observation group, stroke after 90 days of control group, PSD turnover rate is higher than that of the control group, the difference was statistically significant specific(x2=6.62,x2=13.74;P<0.05).Conclusion: PSD can occur in each time period after stroke, most self outcome; super early rehabilitation nursing is helpful to reduce the incidence of PSD risk.

The relevant literatures were looked up, summarized, classified and reviewed, and the research progress in decolorization methods for the extracts of traditional Chinese medicines containing polysaccharide was introduced.The decolorization methods commonly used for polysaccharide extracts of traditional Chinese medicines were activated carbon decolorization, macroporous resin decolorization, hydrogen peroxide decolorization, sodium hypochlorite decolorization and so on.According to the combination form of polysaccharide and pigment, the suitable decolorization method should be selected to improve the purity of polysaccharide and the quality of related preparations, which shows guidance effect on fine finishing of traditional Chinese medicines.

Objective To explore the correlation of electrophysiological features with clinical manifestation in patients with diabetic peripheral neuropathy (DPN).Methods The clinical data of 306 patients with DPN undergoing nerve conduction study (NCS) during May 2013 to June 2015 were retrospectively analyzed.Among 306 patients,304 were type 2,and 2 were type 1 diabetes with a mean age of 64.1 years and disease duration of 1 week-31 years.The relationship between the electrophysiological features and clinical manifestations was evaluated.Nerve conduction studies and F-wave and in sympathetic skin response were analyzed.Results ① NCS in lower limbs presented more abnormalities than that in upper limbs.There was the highest prevalence of not elicited sensory potential in Tibial nerve [56.9％(174/306),x2 =175.9] and the highest prevalence of not elicited motor potential in peroneal nerve [7.8％(24/306),x2 =1 103.1].Amplitude abnormality was more severe in sensory nerve than that in motor nerve[41.4％ (507/1 224) vs.10.8％ (132/1 224),x2 =559.2].The ratio of abnormal motor conduction velocity or distal latency was higher than that of amplitude decreased [22.9％ (280/1 224) vs.10.8％(132/1 224),x2 =1 077.4].② The abnormal ratios of the shortest F-wave latency was lower than that of the prolonged distal latency in median and tibial nerve [5.4％ (33/612) vs.20.6％ (126/612),x2 =607.6].③ The abnormal ratio of sympathetic skin response (SSR) in lower limbs were higher than that of upper limbs [40.2％ (246/612) vs.27.3％ (167/612),x2 =129.4,P ＜ 0.001],abnormal ratio of amplitude was higher than that of the prolonged latency [30.1％ (368/1 224) vs.4.7％ (58/1224),x2 =1040.5,P ＜0.001].Among the 99 cases presenting abnormal SSR in upper limbs,32 cases were normal in conventional NCS.Among the 127 cases presenting abnormal SSR in lower limbs,7 cases were normal in conventional NCS.④Disease course and glycosylated hemoglobin values were positively correlated with the distal motor latency,and negatively correlated with the nerve conduction velocity and amplitude.Conclusion Electrophysiological features of DPN is mainly shown in axon of the distal sensory nerve.Long duration and hyperglycemia may lead to more abnormalities in electrophysiological tests.