This study was aimed to select the best microwave extraction craft conditions of polysaccharide from Radix platycodonis. The composite scores of polysaccharide content and yield rate were used as evaluation indexes. By ex-amining the impact of microwave power, microwave time, extraction times and solid-liquid ratio on crafts, the best craft conditions were optimized by orthogonal test. The orthogonal test showed that when the microwave power was 1800 W, microwave time was 8 min, extraction times were 3, solid-liquid ratio was 1:15, the composite scores were the highest and the extraction craft conditions were the best. It was concluded that the crafts are efficient and stable. It had laid a foundation for the further study on polysaccharide from R adix p latyc odonis .

This study was aimed to establish a HPCE method for the content determination of hesperidin and phillyrin in Bao-He-W an (BHW). Fused silica capillary (75 cm í 50 μm) was employed and 30 mmol·L-1 borax so-lution (8% acetonitrile, pH9.64) was served as the running buffer. Other conditions were as follows: electrokinetic injection was 50 kPa í 20 s; analytical voltage was 20 kV; temperature was 20℃; and detection wavelength was 254 nm. The silica capillary was flushed with 0.1 mol·L-1 sodium hydrate and the running buffer for 10 min before each injection, respectively. The results showed that the linearity of hesperidin was in the range of 0.10~2.40 mg·mL-1 (r=0.999 4), the average recovery was 99.85% and RSD=2.34%. The phillyrin was in the range of 0.07~0.84 mg·mL-1 (r=0.999 2), the average recovery was 99.16% and RSD=2.78%. It was concluded that the method was rapid and sensitive. It can be used for the quality control of content determination of hesperidin and phillyrin in BHW.

This study was aimed to investigate the anti-inflammatory, diuretic effect and acute toxicity of Herb of Lanceolate Sedge. The mice were randomly divided into 5 groups, which were the physiological saline group, aspirin group (0.05 g·kg-1), high dose (HD), middle dose (MD) and low dose (LD) of water extract of Herb of Lanceolate Sedge (10, 5, 2.5 g crude drug per kg) group. Intragastric administration of medication was given to mice once a day for 3 days. Auricular swelling induced by dimethyl benzene was used in the model establishment to calculate the swollen ear degree and the swollen inhibiting rate. And rats were randomly divided into 5 groups, which were the physiological saline group, aspirin group (0.05 g·kg-1), high dose (HD), middle dose (MD) and low dose (LD) of wa-ter extract of Herb of Lanceolate Sedge (10, 5, 2.5 g crude drug per kg) group. Intragastric administration of medi-cation was given to rats once a day for 5 days. Carrageenan induced paw edema was used in the model establishment to calculate the paw swollen rate. And rats were randomly divided into 5 groups, which were the physiological saline group, Furosemide tablets group (0.01 g·kg-1), high dose (HD), middle dose (MD) and low dose (LD) of water extract of Herb of Lanceolate Sedge (10, 5, 2.5 g crude drug per kg) group. Intragastric administration of medication was giv-en to rats once a day for 5 days. Water-loaded was used in the model establishment to calculate the 5-hour urine amount. The maximum tolerance dose (MTD) was used in the determination of the acute toxicity. The results showed that the three groups of HD, MD and LD of water extract of Herb of Lanceolate Sedge had obvious inhibiting effects on the swollen ear degree and the swollen inhibiting rate among mice, and the paw swollen rate of rats. It had signif-icant increasing effect on the 5-hour urine amount. The MTD of intragastric administration of water extract of Herb of Lanceolate Sedge was 640 g crude drug per kg for mice. It was concluded that Herb of Lanceolate Sedge dis-played obvious effects on anti-inflammatory and dieresis with low acute toxicity.

Objective To evaluate the efficacy of sequential psychotherapy combined with selective serotonin reuptake inhibitors(SSRI) s on refractory obsessive-compulsive disorder(OCD). Methods 50 patients with refractory OCD were given to sequential psycho therapy in the original basis of the SSRIs drugs treatment. The patients were given psychoanalysis,cognitive behavioral therapy and reality therapy for 8 weeks at the end of in the first stage, second stage and the third stage. The efficacy was assessed by the Yale-Brown Obsessive Compulsive Scale(Y-BOCS) before treatment,8 weeks, 16 weeks, and 24 weeks after treatment, respectively. Results The total score of the YBOCS before the treatment and at the 24th week after treatment was (21.10 ±4.69) and ( 10.18±6. 14). According to the score-reducing rate of the YBOCS , recovery rate was 34.0%, effective rate was 42.0%. Conclusion Sequential psychotherapy combined with SSRIs is an effective treatment for refractory OCD.

This study is to investigate protective effect of safflor yellow B (SYB) against vascular endothelial cells (VECs) injury induced by angiotensin-II (Ang-II). VECs were cultured and divided into six groups: control group, Ang-II group, Ang-II + SYB (1 micromolL(-1)) group, Ang-II + SYB (10 micromolL(-1)) group, Ang-II + SYB (100 micromolL(-1)) group and Ang- II + verapamil (10 micromolL(-1)) group. Except control group, all of VECs in other groups were treated with Ang- II at the final concentration of 0.1 micromolL(-1). Mitochondria membrane potential (MMP) and free calcium concentration ([Ca2+]i) were measured by laser scanning confocal microscopy, and mitochondria complex IV activity was detected by BCA method. The levels of reactive oxygen species (ROS) in VECs were analyzed by fluorescence detector and apoptosis of VECs was observed by flow cytometer. Caspase 3 was determined by Western blotting method. Comparing with control group, Ang-II was able to increase [Ca2+]i and ROS level, decrease MMP level, inhibit complex IV activity and enhance caspase 3 activity in VECs, as a result, enhance apoptosis of VECs. But SYB could significantly reduce the result induced by Ang- II relying on different dosages (P < 0.05 or P < 0.01). SYB was able to eliminate the effect of Ang-II on VECs via regulating [Ca2+]i, mitochondrial structure and function and inhibiting apoptosis.

0.05).Conclusion In-vitro-cultured CB has good effects in the treatment of apoplexy.Neither in-vitro-cultrued CB nor natural CB for apoplexy has obvious adverse reaction.

ObjectiveTo investigate related factors for severe acute pancreatitis (SAP) with sepsis. MethodsA retrospective analysis was performed for the clinical data of 178 SAP patients who were admitted from January 2007 to March 2020, and according to the presence or absence of sepsis, they were divided into sepsis group with 56 patients and non-sepsis group with 122 patients. The t-test was used for comparison of continuous data between the two groups, and the chi-square test was used for comparison of categorical data between the two groups. A logistic regression model was used for multivariate analyses. ResultsThe incidence rate of sepsis was 31.46% in SAP patients. The univariate analysis showed that there were significant differences between the patients without sepsis and those with sepsis in APACHEⅡ score, blood glucose, blood calcium, serum total cholesterol, serum triglyceride, blood urea nitrogen, serum creatinine, serum albumin, admission to the intensive care unit, hypoxemia, deep venous catheterization, mechanical ventilation, surgical procedure, blood purification, indwelling urinary catheterization, and extent of pancreatic necrosis (all P＜0.05). The multivariate analysis showed that APACHEⅡ score (odds ratio ［OR］=6.748, 95% confidence interval ［CI］: 2.191-20.788, P＜0.05), hypoxemia (OR=3.383, 95% CI: 1.112-10.293, P＜0.05), blood glucose (OR=5.288, 95%CI: 1.176-23.781, P＜0.05), extent of pancreatic necrosis (OR=5.523, 95%CI: 1.575-19.360, P＜0.05), and serum creatinine (OR=5.012, 95%CI: 1.345-18.762, P＜0.05) were independent risk factors for infectious SAP with sepsis, while laparoscopic removal of focal necrotic tissue (OR=0.250, 95%CI: 0.066-0951, P＜0.05) was an independent protective factor against SAP with sepsis. ConclusionThere are several important measures to reduce SAP with sepsis, including blood glucose control, protection of the functions of important organs such as lungs and kidneys, application of minimally invasive surgery to remove focal necrotic tissue, and emphasis on the treatment of critically ill patients with a high degree of pancreatic necrosis.

OBJECTIVEThe relationship between occlusion and temporomandibular disorders (TMD) remains disputed. The related epidemiological studies have been carried out focused on the occlusal characteristics in motion, for example in protrusion, retrusion or laterally extension and on some of malocclusion features. Very few studies were carried out on the intercuspal occlusion which is the most important occlusal relationship. Angle's classification of occlusion and the relationship of over-jet and over-bite are two prominent features now generally used in clinical practice for occlusal evaluation. In present study two kinds of features of occlusion were compared among TMD patients, malocclusion patients and university student volunteers, who of the laters represented nature population.

METHODS100 continued cases visiting our TMD Clinic during the year of 2 000 with complain of TMD problem, 100 malocclusion patients in continued orthodontic department records, and 268 university student volunteers were included. All subjects were with complete dentitions, except of age-related third or second molar tooth eruption difference. Study cast were obtained for all 468 subjects involved and following characters were studied. 1. Angle's classification; 2. Over-jet and over-bite relationship. Anteriorly it included cross-bite, cusp-to cusp bite, deep over-bite (without deep over-jet), deep over-jet (with or without deep-bite), open bite, and others. Posteriorly it included cross-bite, reverse cross-bite and others. 3. Abnormal occlusal index: For the purpose of quantitatively comparing the abnormal occlusal relationship of over-bite and over-jet, the occlusal index was introduced. The anterior continued abnormal was scored as 3 while posterior continued oclcusal abnormal scored as 5. The sum of the scores in the same subject was taken as his abnormal occlusion index.

RESULTS1. Ratio of Angle's I patients in malocclusion group was significantly lower than those in TMD group and US group (P < 0.05), while the later two groups had no difference (P > 0.05). 2. The ratios of anterior cross-bite and deep over-jet were higher in malocclusion group than those in other two groups. No significant difference for the other kinds of abnormal over-jet and over-bite relationship were found among the three groups. 3. The abnormal occlusion index of malocclusion group was higher than that of US group, but not than that of TMD group which had no difference with US group either.

CONCLUSIONS1. Aesthetic related anterior abnormal occlusion such as anterior cross-bite and deep-over-jet attract more attention of popular. But they had no relationship with TMD. 2. TMD patients had not significant occlusal features on Angle's classification and over-jet and over-bite relationship compared to malocclusion and nature population subjects.

OBJECTIVE:To study the rational use of antibacterials in inpatients.METHODS:A discussion was made on the use of antibacterials for 4 948 inpatients in 2004 and 5 476 inpatients in 2005 in our hospital,managed in accordance with the requirements for hospital management by level and the Guidelines for Clinical Use of Antibacterials.RESULTS & CONCL- USIONS:The inpatient use of antibacterials in our hospital has been made basically rational through implementation of systematic management,enactment of antibacterials management by level,monitoring on usage of antibacterials and enforcement of warning system for overusing.