Objective To investigate the relationship among the plasma ET,CGRP and chronic atroph-ic gastritis (CAG). Methods One hundred and eighteen cases with GAG, 30 cases with chronic superficial gastritis( CSG) ;30 cases healthy individuals served as control group. The levels of plasma ET,CGRP were measured by radioimmunoassay. Results (1) ET levels were significantly higher in GAG than in CSG and control (P 0. 05) . (2) The levels of plasma ET is statistically different among mild, moderate and severe CAG(P 0.05). (3)The levels of plasma ET were negatively correlated with the levels of plasma CGRP (r = -0. 214,P =0.02). Conclusion It is suggested that ET and CGRP were possibly involved in the pathogenesis of CAG, and their variations in concentrations were related with the extent of CAG.

Bone-like apatite formation on the surface of calcium phosphate ceramics has been believed to be the prerequisite of new bone growth on ceramics and to be related to the osteoinductivity of the material. The research of the factors effecting bone-like apatite formation is a great help in understanding the mechanism of osteoinduction. This paper is aimed to a comparative study of in vitro formation of bone-like apatite on the surface of dense and rough calcium phosphate ceramics with SBF flowing at different rates. The results showed that the rough surface was beneficial to the formation of bone-like apatite, and the apatite formed faster in 1.5 SBF than in SBF. Rough surface, namely, larger surface area, increased the dissolution of Ca2+ and HPO4(2-) and higher concentration of Ca2+ and HPO4(2-) ions of SBF and was in turn advantageous to the accumulation of Ca2+, HPO4(2-), PO4(3-) near the ceramic surface. Local supersaturating concentration of Ca2+, HPO4(2-), PO4(3-) near sample surface was essential to nucleation of apatite on the surface of sample.
Apatites ; Biocompatible Materials ; Calcium Phosphates ; Ceramics ; Materials Testing ; Surface Properties

This study aimed at investigating the influence of the flow rate of simulated body fluid (SBF) (2 ml/100 ml.min) of body fluid in skeletal muscle upon the formation of bone-like apatite on porous calcium phosphate ceramics. The in vitro immersion experiment in SBF flowing at normal physiological rate is referred to as dynamic SBF. The results showed that bone-like apatite could only formed in the pores of porous calcium phosphate when SBF flow at physiological rate (2 ml/100 ml.min) of body fluid in skeletal muscle. At the same time, bone-like apatite could form both in the pores and on the surface of the samples if the flowing physiological solution is 1.5 SBF. When the flowing speed of SBF is higher than normal physiological speed (10 ml/100 ml.min), no bone-like apatite could be detected both on the surface and in the pores of the materials. This result is in concordance with animal experiments. The dynamic SBF simulates the biological environment of bone-like apatite formation in body better than static SBF (SBF does not flow). This method is very useful for the research of the mechanism of bonelike apatite formation, which is the key step of bone growth on biomaterials, and can be used as an effective approach to investigate mechanism of the osteoinduction of calcium phosphate in nonosseous tissues in vivo.
Apatites ; chemistry ; Body Fluids ; chemistry ; Bone Substitutes ; chemical synthesis ; chemistry ; Calcium Phosphates ; chemistry ; Ceramics ; chemistry ; In Vitro Techniques ; Materials Testing ; Surface Properties

Bone-like apatite formation on the surface of calcium phosphate ceramics has been believed to be necessary for new bone to grow on the ceramics and to be related to the osteoinductivity of the material. The research of bone-like apatite formation is a great help to understanding the mechanism of osteoinduction. Synthetic porous calcium phosphate ceramics (HA/TCP = 70/30) were implanted intramuscularly in pigs, dogs, rabbits and rats to make a comparative study of the bone-like apatite formation onto the porous HA/TCP ceramics in different animals. Specimens were harvested at 14 days after implantation. Samples were detected for the surface morphology with SEM. The chemical composition of the sample surface after implantation was analyzed with reflection infrared (R-IR). Obvious bone-like apatite formation could be detected in the sections of porous specimens harvested from all animals after 14 days intramuscular implantation. Crystal deposition could be only observed on the surface of the concave regions of the samples collected from dogs, rabbits and rat. On the contrary, evenly distributed flake-shaped crystal could be found on the pore surface and also on the outer surface of the materials implanted in pigs. The morphology of bone-like apatite in pigs was different from that in the others animals. Bone-like apatite was not observed in dense specimen implanted intramuscularly. Bone-like apatite formed faster on specimens implanted in rabbit than that in other animals. This formation sequence is different from the sequence of osteoinductivity of biphasic calcium phosphate ceramics implanted in these animals. The results demonstrated that the formation of bone-like apatite on materials is a prerequisite condition to their osteoinduction but other factors also play important roles in osteoinduction.
Animals ; Apatites ; chemical synthesis ; Body Fluids ; chemistry ; Bone Substitutes ; chemical synthesis ; chemistry ; Calcium Phosphates ; chemistry ; Ceramics ; chemistry ; Dogs ; Male ; Materials Testing ; Prostheses and Implants ; Rabbits ; Rats ; Swine

目的：探讨miRNA-490-3p调控Smad2/TGF-β对结直肠癌奥沙利铂化疗敏感性的影响。方法：选取100例结直肠癌（colorectal cancer，CRC）根治性手术后奥沙利铂（oxaliplatin，OXA）同种联合化疗患者作为研究对象，根据化疗情况分为耐药组（n=40）和非耐药组（n=60），用qPCR检测两组外周血miRNA-490-3p水平；选取人CRC细胞株SW480和CRC奥沙利铂（OXA）耐药型细胞株OXA-SW480进行研究，先用qPCR检测两种细胞株中miRNA-490-3p表达水平；后将miRNA-490-3p过表达载体转染OXA-SW480（过表达组），并设立空载体组（空载体转染OXA-SW480）和空白对照组（OXA-SW480未经任何处理）。用CCK-8检测各组细胞增殖能力，同时用不同浓度OXA处理各组细胞，计算其半数抑制浓度（IC50）；用Annexin-V-FITC/PI染色流式细胞术检测各组细胞凋亡率；用WB检测各组Smad2和TGF-β蛋白表达水平。结果：在CRC患者中，耐药组外周血miR-490-3p水平显著低于非耐药组，在CRC细胞株中，OXA-SW480耐药株细胞miR-490-3p水平显著低于正常株SW480细胞（P<0.05）。与空载体组和空白对照组相比，过表达组miR-490-3p水平显著降低（均P<0.05），增殖抑制率显著升高（均P<0.05），细胞凋亡率显著升高（均P<0.01），Smad2蛋白水平显著降低（均P<0.05），TGF-β蛋白水平显著升高（均P<0.05）。经OXA处理后，过表达组的IC50显著低于空载体组和空白对照组（均P<0.05）。经Pearson相关法分析，miR-490-3p与Smad2的表达呈负相关（r=–0.943，P<0.01），miR-490-3p与TGF-β的表达呈正相关（r=0.961，P<0.01）。结论：过表达miRNA-490-3p可增加CRC SW480细胞的OXA敏感性，此作用Smad2/TGF-β信号通路有关。

Objective To investigate the approaches,techniques and efficacies ofmicrosurgical resection ofpetroclival meningiomas.Methods A total of 59 patients with petroclival meningiomas,admitted to our hospital from January 2003 to January 2016,were chosen in our study;single microscopic resection was performed in 55 patients (93.2％) and re-operation was performed in 4 patients (6.8％);29 times (46.0％) via retrosigmoid approach,17 times (27.0％) via tranpetrosal approach,and 17 time via subtemporal approach (27.0％) were chosen.The clinical data,radiological findings,surgical records and outcomes of patients were retrospectively analyzed,and the prognostic factors was analyzed.Results Complete resection was achieved in 31 patients (52.5％),subtotal resection in 19 (21.7％) and partial resection in 9 (15.3％).There was no death in perioperative period.Follow-up were obtained in 55 patients,and median follow-up was 42.6 months (ranged 3-131 months).Permanent neurological damage occurred in 16 patients (27％).Tumor recurred in 11 patients:6 had gamma knife radiosurgery,one had routine radiotherapy,and 4 required re-operation.Two patients died of tumor recurrence/progression reoperation.In these 55 patients,42 enjoyed good prognosis and 13 had poor prognosis.The tumor sizes,brainstem edema and preoperative Kamofsky performance scale scores were unfavorable prognostic factors.Conclusion By using appropriate approaches,surgical strategies and techniques,the petroclival meningiomas can be removed with relatively favorable outcome.

Background/Aims: Metabolic dysfunction-associated steatotic liver disease (MASLD) has become an increasingly important health challenge, with a substantial rise linked to changing lifestyles and global obesity. Ursolic acid, a natural pentacyclic triterpenoid, has been explored for its potential therapeutic effects. Given its multifunctional bioactive properties, this research further revealed the pharmacological mechanisms of ursolic acid on MASLD. Methods: Drug target chips and bioinformatics analysis were combined in this study to explore the potential therapeutic effects of ursolic acid on MASLD. Molecular docking simulations, surface plasmon resonance analyses, pull-down experiments, and co-immunoprecipitation assays were used to verify the direct interactions. Gene knockdown mice were generated, and high-fat diets were used to validate drug efficacy. Furthermore, initial CD4+ T cells were isolated and stimulated to demonstrate our findings. Results: In this study, the multifunctional extracellular matrix phosphorylated glycoprotein secreted phosphoprotein 1 (SPP1) was investigated, highlighting its capability to induce Th17 cell differentiation, amplifying inflammatory cascades, and subsequently promoting the evolution of MASLD. In addition, this study revealed that in addition to the canonical TGF-β/IL-6 cytokine pathway, SPP1 can directly interact with ITGB1 and CD44, orchestrating Th17 cell differentiation via their joint downstream ERK signaling pathway. Remarkably, ursolic acid intervention notably suppressed the protein activity of SPP1, suggesting a promising avenue for ameliorating the immunoinflammatory trajectory in MASLD progression. Conclusions Ursolic acid could improve immune inflammation in MASLD by modulating SPP1-mediated Th17 cell differentiation via the ERK signaling pathway, which is orchestrated jointly by ITGB1 and CD44, emerging as a linchpin in this molecular cascade.