Intestinal microflora play a significant role in regulating systemic immune function,maintaining the intestinal barrier,and preventing the invasion of external pathogenic bacteria.Recent epidemiological studies and experimental research suggest that the occurrence and development of allergic diseases is associated with the disturbances in the intestinal microflora during early life.Research has been taken to evaluate the prevention and treatment effects in allergic disease of probiotics,which is currently unestablished.

Objective To analyze the related factors of local recurrence in patients with rectal cancer after Dixon operation. Methods A retrospective analysis of clinical data of 100 patients from September 2005 to September 2007 in our hospital by TME standard surgicai treatment of low rectal cancer was carried out. Results Of 100 patients, the pre-sacral recurrence rate was 10. 0%, with gender, age, tumor size, tunor invasion lumen circumference,depth of invasion,lymph node metastasis and Duke's stage has nothing to do with the gross type,histological type and lumen,peritoneal tumor cell shedding,and as an independent prognostic factor. Of 90 patients without local recurrence,the 5-year survival rate was 76. 0% ,haff of the survival of 62 months;10 cases of local recurrence,5-year survival rate was 5. 0%, half of the survival of 24 months, suggested that local recurrence in patients influenced the prognosis. Conclusion The low local recurrence of rectal cancer related factors were gross type,histological type and the intestine, peritoneal tumor cells shed, seriously affect the prognosis of patients with local recurrence.

OBJECTIVE To study the inhibitory effects of the water extractions from ordinary green tea,black tea,chrysanthemum tea,oolong tea and Puer tea on digestive tract bacteria.METHODS Incubating plate with foveolae was used to study the inhibitory effect on Vibrio parahaemolyticus,Escherichia coli,Salmonella and Shigella with the water extractions of the tea.RESULTS It is showed inhibitory effect on V.parahaemolyticus by the first water extraction from ordinary green tea,black tea,chvysanthemum tea,oolong tea and Puer tea.Same effect was also observed to E.coli,Salmonella and Shigella.The green tea and Puer tea showed best inhibitory effects on V.parahaemolyticus,and the inhibitory zone diameter of the first water extraction of 2.5mg/ml teas were 10.6mm and 12.3mm,respectively.CONCLUSIONS The first water extraction of many kinds of the tea showes inhibitory effects on the digestive bacteria.

Objective To study the effects of ropivacaine on the duration of labor and mode of delivery in the primigravidas using patient-controlled epidural analgesia (PCEA). Methods Retrospective analysis was performed. The 190 healthy, full-term, and single-fetus parturient primigravidas who received PCEA with 0.1% ropivacaine+fentanyl (1 ?g/ml ) were in the epidural analgesia group. Another 222 primigravidas who didnot receive PCEA were in the control group. The duration of labor and modes of delivery, and the neonatal Apgar scores in both two groups were recorded and evaluated. Results Those in the epidural analgesia group experienced a significantly longer first stage [(426?161) minutes], longer second stage [(54?27) minutes] and longer full duration of delivery [(489?166) minutes] than those in the control one [(409?170) minutes, (364?167) minutes and (37?22) minutes]. The rate of using pitocin in the epidural analgesia group (30.2 %) was significantly higher than that in the control group (4.1%). The cesarean section rate in epidural analgesia group (20.0 %) was lower than that in the control one (28.4%); while the rate of instrumental delivery in the epidural analgesia group (20.0%) was significantly higher than that in the control one (6.3%). In summary, there were significant differences between two groups in the duration of labor, the rate of using pitocin, the rate of instrumental delivery and the rate of cesarean section. But there were no differences found for those newborn who had Apgar scores less than 7 at the point of both one and five minutes (7.9% and 4.5%, 2.6% and 0.5% respectively). Conclusion Epidural ropivacaine labor analgesia lengthens the duration of labor and increases the rate of instrumental delivery, but it has no significant negative effects on the neonates.

Objective To establish an effective cleaning process for automatic spray cleaning machine in case of without water purification system and to reduce the formation of scale on the surface of machine and instruments with increased cleaning degree.Methods Descale process was added to the previons concrete cleaning process.The clean effect Was compared with previous concrete process.The blocking of the pipeline was observed.Results The clean degree by new cleaning process reached 97.23%,which was,better than that of the previous process.The problem of blocking of the pipeline was also solved.Conclusions Addition of descale process when using automatic spray cleaning machine in case of without water purification system could guarantee the clean quality of instruments.

Objective To observe the anti-shock effect of rapid infusion of 7.5% sodium chloride(NaCl) in dog with hemorrhagic shock. Methods Five mongrel dogs were bled to mean arterial pressure(MAP) of 5.33～6.70kPa, and this pressure was maintained for 1 hour. Each dog received 7.5% NaCl (4ml/kg) intravenously, the infusion time was 2 min. The observation time was 4 hours. Results During the infusion period, the blood pressure was obviously lower than that of the shock period(P

Objective To investigate the relationship between plasma rennin levels in ischemic stroke patients and aspirin-in-duced gastrointestinal bleeding .Methods 50 ischemic stroke cases in neurology department of the Second Affiliated Hospital of Chongqing Medical University from June 2012 to December 2012 were collected and divided into 2 groups depended plasma rennin levels :low rennin group and high rennin group .Low rennin group contained 21 cases and high rennin group containd 29 cases .Ap-pearance of haematemesis ,melena and fecal occult blood were considered as positive identification ,peptic ulcer bleeding in endoscope or death as the endpoint event in 1 year follow-up .Comparison of the incidence of gastrointestinal bleeding between 2 groups was made .Results The average age of patients in low rennin group was higher than that in high rennin group(P<0 .05);blood pres-sure of patients in low rennin group was higher than that of high rennin group(P<0 .05);gastrointestinal bleeding rate of low ren-nin group was higher than that of high rennin group(P>0 .05) .Conclusion Different plasma rennin levels in ischemic stroke pa-tients may be unrelated to aspirin-induced gastrointestinal bleeding .

Efficacy of chemotherapy for advanced gastric cancer is unsatisfactory. Molecular targeted therapies have emerged as a novel approach to the treatment of advanced gastric cancer in recent years.These therapeutic strategies include targeting EGFR signal transduction pathway,anti-angiogenesis therapy,multi-target molecular targeted drugs. Targeted drugs such as trastuzumab,cetuximab,bevacizumab,apatinib,sorafenib sunitinib,lapatinib and everolimus which are targeting HER2,EGFR,VEGF and mTOR pathway,are applied in the comprehensive treatment of advanced gastric cancer,and their therapeutic effects are encouraging.

Ticks transmit various diseases to livestock and human beings. The researches on ticks are of great importance in medical and veterinary sciences. To express a glutathione peroxidase gene (HlGPx) from Hemaphysalis longicornis in Escherichia coli (E. coli) so as to provide basis for further studies, the gene was amplified from cDNAs of H. longicornis adult ticks by PCR prompted by information from EST library. The TGA codon encoding selenocysteine in the gene was mutated into the universal genetic code for cysteine,TGC, by site-directed mutagenesis. E. coli was transformed with the recombinant expression vector pGEX-4T-1/HlGPx'(the mutated HlGPx) and induced to express recombinant HlGPx', which was then analyzed by sodium dodecylsulphate-polyacrylamide gel electrophoresis and Western blotting. The result showed that the complete coding sequence of HlGPx was obtained successfully, the deduced polypeptide was 223 aa, with a calculated molecular mass of 25.0 kDa; the recombinant fusion protein was approximately 51 kDa, correspondent to the calculated. The antibody against glutathione S-transferase (GST) recognized the recombinant protein fused with GST in a Western blotting assay, which confirmed the result above-mentioned. In conclusion, the mutated HlGPx was expressed in E. coli successfully and it could be used for further preparation of immune serum and functional analysis.

Objective To examine the relationship between UAE and fibrinolytic activity in patients with type 2 diabetes.Methods 129 type 2 diabetic patients recruited and subgrouped by UAE,and the UAE,FBS,lipids,renal function and the activity of t-PA and PAI-1 were conducted in these patients and 40 health people.Results Compared to control group,the activity of PAI-1 was increased and the activity of t-PA decreased dramaticlly in type 2 diabetic group(P＜0.05);there was significant difference in the ratio of PAI-1 and t-PA between three diabetic groups(P＜0.05),of three group the ratio of PAI-1 and t-PA was most highest in the patients with macroal buminuria,and simple correlation analysis showed positive correlation between UAE and the ratio of PAI-1 and t-PA,especially in the patients with microalbuminuria(r=0.321,P＜0.05).Conclusion The UAE is closely relatedto fibrinolytic activity in patients type 2 diabetes.