Objective:To detect the anti-cancer component, trans-resveratrol in red wine by high performance liquid chromatography, and to determine the influence of environmental factors on its content. Methods: A method for determining trans-resveratrol in red wine by HPLC is described. The operating conditions were ZORBAX-C18 column(4.6 mm×250 mm, 5μm) at room temperature, 30%acetonitrile as mobile phase at a flow of 1ml/min and UV detection at 306 nm. Results:The content of trans-resveratrol in Changyu dry red wine and Great Wall Claret Cabernet are (1.01±0.03)mg/L and (2.36±0.02)mg/L, respectively. The results show that, sunlight, high temperature and poor seal condition can decrease the content of trans-resveratrol. Conclusion:The content of trans-resveratrol varies in different brands of red wine. Red wine should be stored in cool, dark and sealed conditions.

To introduce famous TCM doctor's experience of peripheral facial paralysis diagnosed and treated by Yifeng (TE 17). Based on holism concept of TCM, Doctorrefers to modern medicine and takes Yifeng (TE 17) as the main acupoint for its diagnosis, treatment and prognosis. She determines severe degree according to the pressing pain of Yifeng (TE 17) and the significance of positive substances. She inserts the needle at the acupoint to pharynx and larynx, with 60°~80° from skin. The inserting method is to ensure quickarrival and safety. Besides, the manipulations are various according to different stage principles.

Objective:To construct a mutispecific internal control for competitive RT-PCR analysis of Fas、FasL、GB、P、TIA-1 and ?-actin.Methods:Invitro synthesized fragments were amplified by PCR,there are two products,of which 145 and 147 bp.The 145 bp one contained 5′ primer sequences of Fas、FasL、GB、P、TIA-1 and ?-actin,another contained 3′ primer sequences of the same genes.The products with restriction sites were inserted into the vector PKF_3.Results:Restriction enzyme analysis and DNA sequencing were used to identify the recombinant plasmid,corresponding internal control was obtained by the amplification of the recombinant plasmid with each primer pair.The mutispecific internal control was then used for quantitative detection of TIA-1 in peripheral blood leukocytes from a patient with acutely rejecting allograft.Our study on Fas、FasL、GB、P、TIA-1 and ?-actin showed that the coamplified templates accumulated in a parallel manner throughout not only the exponential phase.Conclusion:The mutispecific internal control can be used for quantitative detection of the six genes. [

Objective To evaluate the effects of alendronate sodium on bone mineral density (BMD) in maintenance haemodialysis (MHD) patients with osteoporosis. Methods Twenty-eight MHD patients with osteoporosis diagnosed by dual energy X-ray absorptiometry were randomly divided into control group (n=15) and treatment group (n=13). Patients in treatment group were treated with oral 70 mg alendronate sodium once a week for 18 months. BMD of hip and lumbar spine was measured by dual energy X-ray absorptiometry at baseline and the end of the 6th, 12th and 18th month. Parathyroid hormone, calcium, phosphorous, alkaline phosphatase levels, blood routine, hepatic and renal function were assayed at baseline and the end of the 18th month, Kt/V was calculated, new bone fracture was recorded. Results The BMD, T-scores and Z-scores in the lumbar spine and specific regions of the hip were stable in the treatment group and obviously decreased in the control group (P<0.01). New bone fracture was found in 1 patient of the treatment group and 5 patients of the control group. The side-effect of alendronate sodium was epigastric discomfort in 1 cases. Conclusions Oral alendronate sodium appears to be well tolerated in MHD patients and keep the BMD stable in the lumbar spine and specific regions of the hip.

AIM: To evaluate the effects of propofol and desflurane on intracranial pressure in patients undergoing gynecological laparoscopic surgery by measuring the ratio of optic nerve sheath diameter (ONSD) to transverse diameter of the eye (ETD). METHODS: Forty patients, scheduled for elective gynecological endoscopic surgery, were randomly divided into propofol group (group P) and desflurane group (group D) (n b 20). Anesthesia maintenance: propofol was injected intravenously in group P, desflurane was inhaled in group D. Remifentanil was injected intravenously and rocuronium was added on schedule. The patients were mechanically ventilated in volume-controlled mode. ETD was recorded after induction of anesthesia. ONSD, HR, MAP, peak airway pressure (Ppeak) and P

Objective: To observe the temperature changes of skin on patients with cold and dampness syndrome by using the non-contact infrared human body thermometer and infrared thermal imager to treat the patients with cold and dampness syndrome by Jin-Bohua national famous Chinese medicine practitioners using the "Mountain Burning Manipulation by Jin-Shi" method to clarify the clinical thermal effects of the "Heat-producing Needling" method. Methods: This study included sixty patients with cold and dampness syndrome who met the enrollment conditionsfrom November 2015 to October 2017. In a room with fixed temperature, the patients were treated by "Heat-producing Needling" method. By using a non-contact infrared human body thermometer and infrared thermal imaging technology, this study observed the skin temperature changes in Quchi and Hegu, Zusanli and Chongyang before needling, immediately afterneedling, 15 minutes after needling, and 30 minutes after needling. Results: After the "Mountain Burning Manipulation by Jin-Shi" treatment, the temperature of local skin surface at acupoints of upper and lower limbs increased with different degrees and the skin temperature reached the highest level when the needle was kept for 15 minutes. The upper limbs' result indicated that skin temperature increased with significant differences in acupuncture of Quchi point for immediately, 15 minutes and 30 minutes as compared with that before acupuncture. It was found that the skin temperature at Hegu point for 15 minutes have a significant increase than before and immediately after acupuncture. The skin temperature of Hegu point lasted 30 minutes, which was significantly higher than before and immediately after acupuncture. Compared with Hegu point, Quchi point has higher skin temperature immediately after acupuncture (t=3.793, P=0.034). The lower limbss' results indicated that skin temperature at Zusanli point increased with significant differences in Zusanli point for immediately needling, 15 minutes and 30 minutes as compared respectively with that beforeneedling. It was found that the skin temperature at Chongyang point for 15 minutes had a significant increase than before and immediately after acupuncture. The skin temperature at Chongyang point for 15 mins had a significant increase than before and immediately afterneedling. The skin temperature of Zusanli point was higher than that of Chongyang point (t=3.794, P=0.034) immediately afterneedling. Conclusions This study demonstrates that the "Mountain Burning Manipulation by Jin-Shi" method has a clear clinical thermal effect on both local and peripheral longitude of patients with cold dampness syndrome.

OBJECTIVETo compare the efficacy of intracoronary administration of combined high-dose adenosine and tirofiban versus intracoronary tirofiban during primary percutaneous coronary intervention (PCI) in patients with acute myocardial infarction.

METHODSConsecutive 258 patients with acute ST-segment elevation myocardial infarction (STEMI) underwent primary PCI, treated with thrombus aspiration and then intracoronary tirofiban, were randomly divided into adenosine group (n = 130) and control group (n = 128). Adenosine group received 2 times intracoronary adenosine (2 mg) after thrombus aspiration and after stenting of the infarct-related artery through the aspiration catheter. Control group received placebo. The primary end point was myocardial blush grade (MBG) after PCI. Secondary end points were thrombolysis in myocardial infarction (TIMI) flow grade and corrected TIMI frame count (CTFC) after PCI, ST-segment elevation resolution (STR), and major adverse cardiac events (MACE) at 30 days and 12 months.

RESULTSTIMI flow grade post PCI did not differ between the 2 groups, while CTFC favored the adenosine-treated patients [(21.6 ± 6.5) frames] compared with the placebo-treated patients [(25.1 ± 7.8) frames, P = 0.001]. MBG 3 was more frequently observed in the adenosine compared to the control group [45.1% (55/122) vs.32.0% (39/122), P = 0.035]. Patients in the adenosine group had a trend of higher rate of compete STR after the procedure compared patients in the control group [53.6% (67/125) vs. 41.9% (52/124), P = 0.065]. The incidence of MACE was comparable between patients randomized to adenosine and placebo at 30 days [12.3% (16/130) vs. 17.2% (22/128), P = 0.295] and at 12 months [12.3% (16/130) vs. 18.0% (23/128), P = 0.227].

CONCLUSIONIntracoronary administration of high-dose adenosine combined with tirofiban provides further improvement on myocardial perfusion after primary PCI but does not affect the clinical outcomes in patients with STEMI.

Adenosine ; therapeutic use ; Aged ; Angioplasty, Balloon, Coronary ; Female ; Humans ; Male ; Middle Aged ; Myocardial Infarction ; therapy ; Percutaneous Coronary Intervention ; Platelet Aggregation Inhibitors ; therapeutic use ; Tyrosine ; analogs & derivatives ; therapeutic use

At present, there is still no general consensus on the thoracic lymph node classification and dissection standard of esophageal cancer, and its indications, surgical approaches, harvested number and scopes are still the debated focuses in the academic circle. Therefore, the Society of Esophageal Tumor, Chinese Anti-Cancer Association organized experts in the field to write the Chinese expert consensus on mediastinal lymph node dissection in esophagectomy for esophageal cancer (2017 edition) based on clinical experience and current available evidence. This article focuses on the surgical approach and recurrent laryngeal nerve lymph node dissection in esophageal cancer. The right thoracic approach is recommended for wide application in clinical practice by the authors because of the high resection rate and the advantages of more stations and higher number. But the left thoracic approach should not be eliminated and can be used cautiously to some particular patient. Because the metastatic rate of bilateral recurrent laryngeal nerve lymph node in thoracic esophageal cancer is very high, it is classified as the first and the second group of thoracic lymph nodes. Hence, the authors strongly recommend that bilateral recurrent laryngeal nerve lymph node dissection should be performed for all the esophageal cancer patients. The 3-field or 2 and a half field dissection can be performed by right thoracic approach. The bilateral recurrent laryngeal nerve lymph nodes must be cleaned, and the decision of neck dissection should be made accordingly.
Consensus ; Esophageal Neoplasms ; surgery ; Esophagectomy ; Humans ; Lymph Node Excision ; Lymph Nodes ; Lymphatic Metastasis ; Retrospective Studies

Humans ; Multiple Myeloma/diagnosis* ; Plasma Cells ; Bone Marrow

OBJECTIVETo investigate the incidence of surgical site infection (SSI) and risk factors in colorectal cancer surgery patients.

METHODSBetween October 2003 and October 2013, 1 381 consecutive patients with colorectal cancer managed surgically with primary anastomosis were included in the study. There were 762 male and 619 female patients with mean body mass index (BMI) was (27.7 ± 3.7) kg/m², aged from 20 to 90 years with a median of 67 years. Patients undergoing emergency surgery and requiring stoma creation were excluded. The patients' characteristics, surgical conditions and prognosis were recorded. Univariate and multiple logistic regression analysis were used to identify any variable predictive factors of SSI.

RESULTSOne hundred twenty-six (9.12%) cases developed incisional SSI. The occurrence time for SSI was from 2 to 20 days, mean (6.7 ± 2.9) days. According to multivariable logistic regression analysis, BMI (OR = 1.058, P = 0.030), intraoperative contamination (OR = 10.549, P = 0.000) and open operation as compared with a laparoscopic procedure (OR = 2.111, P = 0.001) were significant independent predictors of incisional SSI. There was a significant decrease in incisional SSI in wound protectors group (OR = 1.646, P = 0.012).

CONCLUSIONBMI and intraoperative contamination are independent predictors of incisional SSI, and wound protectors and laparoscopic surgery are associated with a lower incidence of incisional SSI following colorectal cancer surgery.

Adult ; Aged ; Aged, 80 and over ; Anastomosis, Surgical ; Body Mass Index ; Colorectal Neoplasms ; surgery ; Colorectal Surgery ; Female ; Humans ; Logistic Models ; Male ; Middle Aged ; Prognosis ; Retrospective Studies ; Risk Factors ; Surgical Wound Infection ; etiology ; Young Adult