1.Effects of different anti-rejection drugs and projects on dyslipidemia after organ transplantation
Mengjuan XUE ; Chaoyang LYU ; Yao ZHANG ; Shunmei HE ; Mingxiang YU
Chinese Journal of Endocrinology and Metabolism 2015;(9):816-818
[Summary] Dyslipidemia after organ transplantation is one of the important risk factors of postoperative cardiovascular disease and graft dysfunction. There are many factors that result in postoperative dyslipidemia. However, the factors influencing serum lipid levels are changing with the development of organ transplantation. In this article the effects of different anti-rejection drugs such as cyclosporine, azathioprine, mycophenolate mofetil, tacrolimus, rapamycin ( sirolimus ) , corticosteroids, and monoclonal antibody on dyslipidemia after organ transplantation were summarized in different eras.
2.Clinical characterization and genetic testing for a patient with creatine deficiency syndrome 1.
Shu XYU ; Chen XU ; Yuan LYU ; Chuang LI ; Caixia LIU
Chinese Journal of Medical Genetics 2022;39(2):213-215
OBJECTIVE:
To explore the genetic basis for a child affected with cerebral creatine deficiency syndrome 1 (CCDS1).
METHODS:
High-throughput sequencing was carried out to screen pathogenic variant associated with the clinical phenotype of the proband. The candidate variant was verified by Sanger sequencing.
RESULTS:
High-throughput sequencing revealed that the proband has carried heterozygous c.327delG variant of the SLC6A8 gene, which was verified by Sanger sequencing.Neither parent was found to carry the same variant.
CONCLUSION
The de novo heterozygous c.327delG variant of the SLC6A8 gene probably underlay the CCDS1 in this child.
Brain Diseases, Metabolic, Inborn/genetics*
;
Creatine
;
Genetic Testing
;
Heterozygote
;
Humans
;
Mental Retardation, X-Linked
;
Mutation
3.Clinical feature and genetic analysis of a fetus with autosomal recessive polycystic kidney disease.
Shu XYU ; Chen XYU ; Yuan LYU ; Chuang LI ; Caixia LIU
Chinese Journal of Medical Genetics 2021;38(9):880-883
OBJECTIVE:
To explore the genetic etiology of a fetus with autosomal recessive polycystic kidney disease (ARPKD).
METHODS:
Prenatal ultrasonography has revealed oligohydramnios and abnormal structure of fetal kidneys. After careful counseling, the couple opted induced abortion. With informed consent, genomic DNA was extracted from the muscle sample of the abortus and peripheral blood samples of the couple. High throughput whole exome sequencing was carried out to detect potential variants in relation with the disease. Suspected variants were verified by Sanger sequencing.
RESULTS:
Prenatal ultrasound revealed increased size of fetal kidneys, with multiple hyperechos from the right kidney, and multiple hyperechos with anechoic masses within the left kidney. DNA sequencing revealed that the fetus has carried heterozygous variants of the PKHD1 gene, including c.7994T>C inherited from its father, and two heterozygous variants of the PKHD1 gene c.5681G>A from its mother.
CONCLUSION
The compound heterozygous c.7994T>C and c.5681G>A variants of the PKHD1 gene probably underlay the pathogenesis of ARPKD in this fetus. Above results can provide guidance for subsequent pregnancies of the couple.
Female
;
Fetus
;
Genetic Testing
;
Humans
;
Mutation
;
Polycystic Kidney, Autosomal Recessive/genetics*
;
Pregnancy
;
Receptors, Cell Surface/genetics*
4. Progress on surgical treatment of intrahepatic cholangiocarcinoma
International Journal of Surgery 2020;47(2):107-112
Intrahepatic cholangiocarcinoma (ICC) is a malignant tumor originating from the liver, which with high malignancy and poor long-term prognosis. Radical resection is the only effective treatment for intrahepatic cholangiocarcinoma. In recent years, with the deepening understanding of the biological behavior and clinical characteristics of intrahepatic cholangiocarcinoma, its clinical stage, surgical indications, surgical methods, lymph node dissection and other surgical treatment strategies have also changed. This article reviews the current status of surgical treatment of intrahepatic cholangiocarcinoma based on the latest clinical research progress.
5.Research progress in perioperative management of portal vein thrombosis in liver transplantation
Organ Transplantation 2024;15(1):26-32
Portal vein thrombosis is one of the common complications of liver cirrhosis. The incidence of portal vein thrombosis is increased with the progression of diseases. The incidence and progression of portal vein thrombosis are associated with multiple factors. The indications of anticoagulant therapy remain to be investigated. At present, portal vein thrombosis is no longer considered as a contraindication for liver transplantation. Nevertheless, complicated portal vein thrombosis will increase perioperative risk of liver transplantation. How to restore the blood flow of portal vein system is a challenge for surgical decision-making in clinical practice. Rational preoperative typing, surgical planning and portal vein reconstruction are the keys to ensure favorable long-term prognosis of liver transplant recipients. In this article, epidemiological status, risk factors, typing and identification of portal vein thrombosis, preoperative and intraoperative management of portal vein thrombosis in liver transplantation, and the impact of portal vein thrombosis on the outcomes of liver transplantation were reviewed, aiming to provide reference for perioperative management of portal vein thrombosis throughout liver transplantation.
6. Safety and efficacy of balloon pulmonary angioplasty in the treatment of chronic thromboembolic pulmonary hypertension
Chaoyang LYU ; Kun GAO ; Qiang HUANG ; Juanni GONG ; Yong ZHANG ; Jianfeng WANG
Chinese Journal of Radiology 2020;54(1):48-52
Objective:
To evaluate the safety and efficacy of balloon pulmonary angioplasty (BPA) in the treatment of chronic thromboembolic pulmonary hypertension (CTEPH).
Methods:
A study was conducted on 25 patients consisting of 10 males and 15 females with CTEPH who received BPA treatment from February 2017 to May 2018 in Beijing Chaoyang Hospital, Capital Medical University. The changes of brain natriuretic peptide (BNP), mean pulmonary artery pressure (mPAP), tricuspid annulus systolic plane excursion (TAPSE), six-minute walking test (6WMD), right ventricular basement diameter and WHO cardiac function grading were compared before and after treatment with BPA using paired
7.Expression profiling of metastasis-related microRNAs in early esophageal squamous cell carcinoma.
Jun LU ; Liyan XUE ; Mulan JIN ; Ning LYU
Chinese Journal of Pathology 2014;43(5):313-317
OBJECTIVETo study the difference of microRNA (miRNA) expression between two groups of early stage (pT1N0) esophageal squamous cell carcinoma (ESCC) patients who had different outcome and the prognostic significance of different miRNA in metastatic of early ESCC, and to identify useful prognostic markers in the selection of appropriate treatment for early ESCC patients.
METHODSTaqMan human miRNA arrays and bioinformatics were used to detect and analyze the expression profiles of miRNAs in the two groups, and RT-PCR was used to verify the differences in miRNA expression.
RESULTSThe miRNA arrays revealed a total of 41 markedly changed miRNAs in the survival group compared with the death group. Bioinformatics analysis, prediction and significant function analyses of targeted genes and pathway analysis identified that miR-27a, miR-143 and miR-886-5p levels were increased or decreased by seven-folds or more. The enriched target genes were GRB2, SOS1, MAPK1, EGFR, CBL, SPRY2, RPS6KA5, IGF1R, NGFR, MAPK14 and CREB1. These genes were significantly related to the following signaling pathways, i.e.Sprouty regulation of tyrosine kinase signals pathway, Erk1/Erk2 Mapk signaling pathway and transcription factor CREB and its extracellular signals.
CONCLUSIONSmiR-27a, miR-886-5p, and miR-143 may be potential prognostic markers of metastasis for early ESCC. The detection of these miRNAs plays a directive role for the treatment options of early ESCC. The regulation of targeted genes and mechanism remain to be further studied.
Biomarkers, Tumor ; genetics ; metabolism ; Carcinoma, Squamous Cell ; genetics ; pathology ; surgery ; Esophageal Neoplasms ; genetics ; pathology ; surgery ; Gene Expression Profiling ; Gene Expression Regulation, Neoplastic ; Humans ; Lymphatic Metastasis ; MicroRNAs ; metabolism ; Neoplasm Metastasis ; Neoplasm Staging ; Signal Transduction
8. Application of vascular replacement technique with allogenic blood vessel in radical resection for pancreatic carcinoma: a report of 33 cases
Shaocheng LYU ; Qiang HE ; Ren LANG ; Lixin LI ; Hua FAN ; Xianliang LI ; Zhihua ZHANG ; Bing PAN
Chinese Journal of Surgery 2018;56(4):274-278
Objective:
To analyze the application of vascular replacement technique with allogenic blood vessel in radical resection for pancreatic carcinoma.
Methods:
The clinical data of 33 patients with vascular invasion of pancreatic carcinoma who underwent radical resection from April 2013 to April 2017 in Department of Hepatobiliary Surgery, Beijing Chaoyang Hospital were retrospectively analyzed. There were 14 males and 19 females with age of (62.5±10.6)years(ranging from 35 to 78 years). Vascular replacement technique with allogenic blood vessel was used on all patients who underwent radical resection for pancreatic carcinoma. The operation procedure was made according to the specific location of the carcinoma, and the allogenic blood vessel was selected according to the type of vascular invasion. The matching vessel was selected for replacement to the patient who was invaded only one vessel. And the "Y" type of iliac vein was selected for replacement to the patient who was invaded the confluence of portal vein, splenic vein and superior mesenteric vein. After the operation, the patients were followed up by telephone and outpatient review.
Results:
All of 33 patients were successfully completed the operations. There were 28 patients underwent pancreaticoduodenectomy with vascular replacement, and 5 patients underwent total pancreatectomy with vascular replacement. All the patients were confirmed pancreatic carcinoma and R0 resection according to the postoperative pathology. There were 16 patients with the carcinoma invasion the confluence of portal vein, splenic vein and superior mesenteric vein, 12 patients with the carcinoma invasion the superior mesenteric vein, and 5 patients with the carcinoma invasion the portal vein. There was no perioperative death in this group and no complications related to allogenic blood vessel. The incidence of postoperative complications was 18.2% (6/33), and the incidence of pancreatic fistula was 6.1% (2/33), all of which were biochemical fistula. There were 32 patients were followed up, and the follow-up rate was 96.9%. The median survival time was 14.6 months. The half-year, 1-year and 2-year survival rates were 75.6%, 37.6% and 27.4%.
Conclusion
The application of vascular replacement technique with allogenic blood vessel for pancreatic carcinoma has a great significance for improving the R0 resection rate and the prognosis of patients.
9.Changes of Th, Treg cytokines and signaling pathway proteins during immune tolerance process in rat models of liver transplantation
Xianliang LI ; Chun BAI ; Long YANG ; Han LI ; Shaocheng LYU ; Jiqiao ZHU ; Jun MA ; Jiantao KOU ; Qiang HE
Organ Transplantation 2019;10(4):416-
Objective To investigate the relationship between immune tolerance and the changes of helper T cell (Th), regulatory T cell (Treg) cytokines, related signaling pathway proteins during immune tolerance process in rat models of liver transplantation. Methods The orthotopic liver transplantation rat models were established by double-cuff technique. All rats were divided into 3 groups. In the operative control group (
10.Clinical application investigation of quantitative evaluation system of immune status in guiding individualized management of immunosuppressants after liver transplantation
Yanan JIA ; Jiqiao ZHU ; Han LI ; Wenli XU ; Ruolin WANG ; Shaocheng LYU ; Jiantao KOU ; Xianliang LI ; Qiang HE
Organ Transplantation 2022;13(6):803-
Objective To investigate the guiding role of quantitative evaluation system of immune status in the individualized management of immunosuppressants for the recipients after liver transplantation. Methods Clinical data of 239 liver transplant recipients were retrospectively analyzed. MingDao Immune Cell Analysis (MICA) was established. All recipients were divided into two groups according to the adjustment regimens of immunosuppressants. The immunosuppressant regimen was adjusted according to MingDao Immune System Score (MISS) in the MISS group (