Objective To investigate the feasibility and effectiveness of laparoscopic splenectomy (LS) in patients with refractory idiopathic thrombocytopenic purpura (ITP) resistant to medical management. Methods Clinical data of 31 cases of refractory ITP (platelet count

Objective To evaluate the perioperative safety of laparoscopic cholecystectomy(LC) in patients with gallstones complicated with end-stage renal disease(ESRD). Methods Clinical data of 41 patients with gallstones accompanying ESRD treated with LC(ESRD Group) between May 1994 and May 2005 were analyzed retrospectively.Another 200 patients without ESRD(non-ESRD Group) receiving LC during the same period were randomly selected for comparison.Results The patients in the ESRD Group were older than those in the non-ESRD Group(49.8?11.9 years vs 44.2?12.3 years;t=2.655,P=0.008).There were significant differences between the ESRD Group and the non-ESRD Group in hemoglobin levels(76.3?11.7 g/L vs 120.1?8.4(g/L)) and platelet count [(141?36)?10~9/L vs(183?51)?10~9/L)] (t=22.905 and 6.226;P

Objective To investigate the outcome of induction of tumor cell apoptosis with low dosage of 131 I labelled anti carcinoembryonic antigen(CEA) monoclonal antibody C50( 131 I C50) and the therapeutic efficacy of combining radioimmunotherapy(RAIT) with chemotherapy in colorectal cancer xenografts. Methods Human colorectal cancer xenografts with positive CEA expression were established in nude mice with LoVo cell line. 5 fluorouracil(5 FU), 75 ?Ci 131 I C50, and 5 FU, combined with 131 I C50 were given to nude mice through tail vein to treat xenografts on 9th day after implantation of tumor cells. Fifteen days after implantation, each mouse was sacrificed and tumor tissues were stained with HE and terminal deoxynucleotidyl transferase mediated X DUTP nick end labeling technique(TUNEL technique). Apoptosis index(AI) of xenograft cells in each mouse was calculated. Results Under light microscope, no obvious cytolysis or necrosis of tumor cells was seen in all four groups. Apoptosis indexes in blank control group, chemotherapy group, radioimmunothera py(RAIT) group, and RAIT+chemotherapy group were (0.29?0.08)%, (18.68? 2.69 )%,(40.88 ?4.54 )% and (62.33?8.00)%, respectively. There were significant difference of apoptosis indexes between any groups( P

Objective To study the effects of electromagnetic field (EMF) exposure on osteoporosis in ovariectomized mice. Methods Sixty 8-week-old female Kunming mice were divided into four groups at random: a sham operation group (group A), an ovariectomized group (group B), an EMF and ovariectomized group (group C) and a nilestriol and ovariectomized group (group D). Bilateral ovariectomies were performed on all mice except those in group A. The mice of group C were exposured to a 15 Hz, 1.0 mT electromagnetic field. The mice of group D were given at nilestriol 1.5 mg/kg/week. The bone mineral density (BMD) of the lumbar vertebrae was measured before the mice were sacrificed at the 12th week. Blood specimens were collected every two weeks to measure the ac-tivity of alkaline phosphatase (ALP) and the concentration of bone gamma-carboxyglutamic-acid-containing proteins (BGP), calcium and estradiol in the serum. Histological sections were taken to examine and analyze the changes in bone trabeculae in the lumbar vertebrae after 6 and 12 weeks. Results EMF at 15 Hz and 1.0 mT intensity signifi-cantly increased the activity of ALP and the concentrations of BGP and calcium in the serum. In addition, the absorp-tion of bone trabeculae in the lumbar vertebrae was significantly restrained. Conclusions EMF at 15 Hz and 1.0 mT can restrain the development of osteoporosis in ovariectomized mice.

Objective To study the effects of an electromagnetic field (EMF) on the expression of fibro-blast growth factor (FGF-2) and it' s receptor (FGFR-2) mRNA in rat bone marrow mesenchymal stem cells (BMSCs) in vitro. Methods Rat BMSCs were isolated and cultured in vitro. The subcultured cells were divided into different groups to be EMF stimulated at 1.0 mT. The expression of FGF-2 and FGFR-2 mRNA were detected by reverse transcription-polymerase chain reaction (RT-PCR). Results Different frequencies and durations of 1.0 mT EMF exposure induced FGF-2 and FGFR-2 mRNA expression in comparison to blank controls. The expression of FGF-2 mRNA reached a peak after stimulation at 15 Hz for 10 min, 50 Hz for 60 min and 75 Hz for 30 min. And the expression of FGFR-2 mRNA reached a peak after 30 minutes at all frequencies. At 1.0 mT with 30 min exposure, the expression of FGF-2 mRNA peaked after 50 Hz stimulation, and the expression of FGFR-2 mRNA peaked after stimulation at 75 Hz. Conclusions Moderate EMF stimulation can significantly increase the expression of FGF-2 and FGFR-2 mRNA in rat BMSCs in vitro.

0.05). Conclusions LS,whereas of less traumatic and low morbidity, results in comparable effects as OS for the treatment of ITP.