Objective To investigate the early and long-term effects of enhanced ischemia/reperfusion injury on the transplanted abdominal aorta. Methods Abdominal aorta grafts from Sprague-Dawley (SD) rats were cold stored for 1 or 24 hours, and they were orthotopically transplanted to SD or Wistar recipients. The pathohistological changes and the expression of TGF-? 1 in the grafts were observed. The levels of serum lipid peroxides before and after transplantation were also measured. Results The intima was significantly thicker in aorta transplants which was cold stored, both in SD→SD and SD→Wistar groups 10 weeks and 6 weeks after transplantation, whereas grafts which were cold stored for 24 hours showed pronounced thickening as early as 2 weeks after transplation. Serum lipid peroxides levels were elevated significantly 2 hours post-transplantation in all groups, but they were lowered 4 and 24 hours post-transplantation. The expression of TGF-? 1 in 24 hours of ischemia became stronger 1 week after transplantation regardless the difference in strains of the animal. Conclusions The enhanced ischemia/reperfusion injury can aggravate the infiltration of inflammatory cells, intensify the expression of TGF-? 1, accelerate the thickening of intimal layer.

Objective To explore the feasibility of B-ultrasound guided ureteroscopic holmium laser nephrolithotomy via percutaneous nephrostomy for the treatment of complex renal calculi. Methods A total of 32 cases of complex renal calculi was included in the study. Under local anaesthesia and B-ultrasound guidance, a percutaneous nephrostomy was performed untill a F_ 16 catheter could be introduced, with the sheath indwelling in the tract. Then under a F_ 8/9.8 ureteroscopy, holmium laser nephrolithotomy was conducted for 1~3 fractions with an interval of 3~5 d. Results The renal puncture was successfully accomplished in all the cases. The stone-free rate was 75% (24/32) at 4 weeks, and 94% (30/32) at 12 weeks after procedure. No serious hemorrhage or infection happened. No conversion to open surgery was required. Conclusions Under local anaesthesia and B-ultrasound guidance, ureteroscopic holmium laser nephrolithotomy via percutaneous nephrostomy for the treatment of complex renal calculi is safe, effective, and feasible.

Objective To investigate the relationship between urine IgG of the first hospitalized children suffered allergic purpura and recurrence within one year. Methods Used immunoturbidimetry to determine acute-stage and convalescent urine IgG of 43 children suffered allergic purpura in the first hospitalized and no recurrence within one year (no recurrence group), and of 29 children suffered allergic purpura and recurrence within one year (recurrence group), and of 28 hospitalized children suffered primary nephrotic syndrome during the corresponding time period before hormonal therapy (nephrotie syndrome group), and of 30 children done medical examination in out-patient clinic (control group), then analyzed.Results There was no significant difference of the urine IgG level in acute-stage between no recurrence group [(1.391± 0.743) g/L] and recurrence group [(1.474 ±0.658) g/L](P>0.05), while they were all lower than that in recurrence group [(2.808 ± 0.683) g/L] (P < 0.01). The level of convalescent urine IgG in nephrotic syndrome group [(0.202 ± 0.154) g/L] was obviously higher than that in no recurrence group [(0.115 ±0.103) g/L] and control group [(0.109 ±0.098) g/L](P<0.05). Conclusion Urine IgG is a significant index to judge the activity and recurrence and prognosis of pedo-allergie purpura.