Objective To develop an advanced three-cuff-technique orthotopic liver transplantation model in rats. Methods The recipient's liver was not resected when the donor's liver was set in position, but was gradually resected during the process of vascular anastomoses. Results This method greatly shortened the anhepatic phase (11.8 min for the mean time), simplified the manipulation. The operative successful rate was 92 % . Conclusion This model could be used as an effective measure for the study of liver transplantation in rats.

Objective To expand the application of electrochemical immunosensor during deleting aflatoxin B1 in foods and feeds through analyzing impacts of the time of antibody incubation and sample preparation .Methods T he double self-assembly immu-nosensor combined with aflatoxin B1 and carboxylated single-walled carbon nanotubes (SWNTs) was characterized by cyclic volta-mmetry and impacts of the time of antibody incubation and sample preparation methods were investigated .Results The signal in-creased gradually following the increasing time of antibody incubation and reached a plateau at 90 min and sample preparation meth-ods showed a comparatively large impact on results .Additionally ,the crude extractions purified through removing interfering com-pounds by immunoaffinity column could effectively eliminate the interference effects of sample matrix .Conclusion Deleting aflatox-in B1 by electrochemical immunosensor is characterized by various features ,such as fast ,simple and low detection limits .The pres-ent study shows that stability of the electrochemical immunosensor is affected by the time of antibody incubation and sample prepa-ration .