Background Open-globe injury of zone Ⅱ is a severe eyeball wound in the sclera area near 5mm from the corneal limbus.To accurately evaluate the local tissue structure after the wounding is very important for lessening the complications of eye injury.Ultrasound biomicroscope (UBM) has been proven useful in examining eyeball wall and vitreous at the wound in order to offer the useful information about whether secondary operation is needed for the injured eyes and when is the optimal timing for surgery.Objective The present study is to explore the clinical application of UBM in open-globe injury of zone Ⅱ. Methods This is an observational study of clinical cases.UBM examination was performed on 38 eyes of 38 cases received emergency repair surgery of open-scleral injury.The restore status of sclera,ciliary body and vitreous of injuried zone (zone Ⅱ) were evaluated under the UBM in 3-5 days after the operation.The comprehensive results of UBM,B-scan and ophthalmoscope examinations were analyzed to determine the further treatment.The oral informed consent was obtained from each subject before this trial.Results Vitreous surgery were performed on 15 cases,and all wounds were found by UBM and were proven in the surgery.The other 23 cases were followed up in out-patient department and no obvious complications were found.Conclusion UBM is a valuable and atraumatic examination for open-globe injury of zone Ⅱ.It offers important and appraisal information about ocular injury.

Objective To investigate the infection situation of group A human rotavirus(HRV)among the infants and young children with diarrhea in Lanzhou city,and to observe the relation between genders and age with the viral positive rate.Methods 2 378 samples of feces in the infants and young children with diarrhea were collected in this hospital from January to December 2013.The group A HRV antigen in samples was detected by using the immunochromatographic sandwich method.The positive rates of group A HRV infection in different months were analyzed and compared with the bacterial diarrhea;the viral positive rates in different genders and ages were analyzed.Results Among 2 378 detected feces samples,the average positive rate of group A HRV was 24.1% and the highest was 39.8% during the months from October to December;the average positive rate of bacterial diarrhea was 28.2% and the highest was 38.2% during the months from July to September.The positive rate of group A HRV was 25.5% in male children patients and 22.6% in female children patients,but the difference was not statistically significant (P >0. 05).The differences in the positive rates of group A HRV among different ages had statistical significance (P <0.05).The average positive rate was highest(29.0%)in children patients aged less than 2 years old,followed by the children patients aged 2 - <4 years old,which was 21.6%.Conclusion The group A HRV has the higher infection positive rate in winter and early spring,which mainly focuses on the children patients aged less than 2 years old.

Objective To report the effect of hormone replacement plus orthopedic surgery for 4 patients with female pseudohermaphroditism caused by congenital adrenal hyperplasia. Methods Four patients with female pseudohermaphroditism caused by congenital adrenal hyperplasia were included.On admission their ages were 11,15,14 and 20 years,respectively.The diagnoses were confirmed by medical histories,ultrasonography,serum levels of hormones and chromosome analysis.They all underwent clitoroplasty and vaginoplasty with perineal skin flap. Results After operation the patients received estrogen and progestogen for a short time to adjust the menstrual cycle and hydrocortisone replacement(10 to 20 mg,twice a day) for their lifetime.Normal menstruation and well-developed breasts were observed in all the patients.No constriction occurred in the reconstructed vaginas. Conclusions This disease can be easily misdiagnosed as hypospadias with bilateral cryptorchidism.Therefore,in addition to careful physical examination,the measurement of serum hormones,ultrasonography of the abdomen,and chromosome analysis are necessary.Proper utilization of the perineal skin flap for reconstruction of the clitoris and vagina as well as careful perioperative managements in combination with hormone replacement therapy can greatly contribute to satisfactory results.

Both single and long-term aerobic exercise may play a role for insulin resistance (IR), however, there is no agreement for in-terval exercise. Simple resistance training is controversial, and the significance of resistance-aerobic exercise for IR is also mixed.

Objective To investigate the reconstruction procedures of digestive tract following total gastrectomy.Methods The clinical data of pylorus ring preserving and jejunum interposition for the reconstruction of digestive tract following total gastrectomy in 17 cases of fundus and/or cardia carcinoma in past 3 years in our hospital were reviewed. Results There was no operative death,and no stomach fistula or constriction occurred in this series.All the 17 patients were discharged with recovery. Half a year after the operation, all the patients can eat about 200-300g each time, and 3-4 times a day. Patients′ subject feeling was good; no retrosternal burning pain occurred after meal; no symptoms of bile reflux or empty disorder happened. Conclusions The reconstruction of digestive tract following total gastrectomy using pylorus ring preservation and jejunum interposition should be effective if strict indications are adopted.

Objective To observe the effects of selenium extracellular polysaccharide of Porphyridium sp(Se-PSP) on the growth and apoptosis of BEL-7402 tumor cell line.Methods The Se-PSP was obtained from a sort of culture medium with some selenous acid solution.In vitro,human tumor cells were cultured in sorts of PSP or Se-PSP liquors with different concentrations,and the result of which was determined by MTT,Focus apoptosis assay and DNA fragmentation.Results and Conclusion Se-PSP(80mg?L-1) displayed strong growth inhibitory effects against BEL-7402 human cancer cell line,and induced the apoptosis of BEL-7402 cells.

Objective To study the effects of the inopolysaccharide from Spirulina maxima (IPSⅡA) on tumor-bearing mice.Mothods The sarcoma 180(S 180) was transplanted in the abdominal cavity and armpit of mouse and then the survival time, the tumor weight, leukocyte count and the immunity index of the tumor-bearing mice treated with IPSⅡA were measured and compared with the control group. Results and ConclusionThe results showed that medication of IPS IIA ip in advance could obviously inhibit the tumor growth and prolong the survival time of the tumor-bearing mice.

Objective To investigate the influence factors of peripheral blood hematopoietic stem cell of healthy donor by granu‐locyte colony‐stimulating‐factors(G‐CSF) mobilization .Methods G‐CSF was subcutaneously injected in 24 cases of healthy donor for mobilizing hematopoietic stem cells .The T lymphocyte subgroup and blood routine data were detected .Results After G‐CSF stimulation ,peripheral blood CD3+ (% ) ,CD3+CD4+ (% ) ,white blood cell (WBC) count and platelet were significantly increased (P<0 .05) .And nucleated cells density of bone ,percentage of CD34+ cells on mobilized 4 ,5 ,6 d had no obvious difference .The correlation analysis showed that gender ,age and weight were negatively correlated with CD 34+cells percentage (P<0 .05) and pos‐itively correlated with WBC count (P<0 .01) .Conclusion Male donor is superior to female donor within a certain range ,the smal‐ler age ,the lighter weight ,the higher WBC count ,the higher the percentage of CD34+ cells in peripheral blood hematopoietic stem cells after G‐CSF mobilization .

ObjectiveTo establish the rapid molecular diagnosis of 16 common coagulase negative Staphylococcus(CNS).MethodsDNA sequencing of 16 CNS would be obtained with gap gene.After the alignment gap gene sequences which were available in the GenBank,the bacteria were identified with homological alignment and phylogenetic tree,and compared with the 16S rRNA gene.ResultsThe sequence similarity of the gap sequences ranged from 39% to 98% in 16 CNS.There were the highest similarity (98%) between S.hominis and S.hominis subsp,and the lowest(39% ) between S.saprophyticus and S.xylosus.The sequence similarity of the 16S rRNA sequences ranged from 96 to 98%,at least two species of bacteria similar rate of 99% and the most four species similar rate of 99%.Phylogenetic homology analysis showed that it was a high confidence(99% ) in the detection ofS.xylosus and S.lentus,S.chromogenes and S.intermedius,S.hominis and S.hominis subsp,but for 10 other species of bacteria,gap homology analysis has less unreliable confidence(49%,56% ) and 16S rRNA has more unreliable confidence(43%,43%,50%,56%,63%,65%,76% ).ConclusionAnalysis of gap sequence could identify 16 CNS timely and accurately,with higher confidence than 16S rRNA.

Objective To investigate whether repeated low-frequency transcranial magnetic stimulation (rTMS) can affect the expression of Kca1.1,Nav1.6,NMDAR1 and/or GAD65 protein in the pyramid layer of the CA3 region of the hippocampus.Methods Fifty rats were randomly divided into an experimental group and a sham group.The former was administered low-frequency rTMS on 14 consecutive days ; the latter were given sham stimulation for 14 days.After the protocols were completed,each group was sub-divided into 6 h,24 h,1 week,3 week and 6 week sub-groups.The rats of each sub-group were sacrificed at the corresponding time points and the expression of KCa1.1,Nav1.6,NMDAR1 and GAD65 in the CA3 region of the hippocampus was examined using immunohistochemical techniques.Results Compared with the sham group,KCa1.1-positive neuron densities increased significantly in the period 6 h to 3 weeks after rTMS.GAD65-positive neuron densities were also elevated significantly from 6 h to 3 weeks.NaV1.6-and NMDAR1-positive neuron densities decreased transiently at 6 h after the completion of the rTMS protocol.Conclusions These results show that low-frequency rTMS can upregulate the expression of KCa1.1 and GAD65,and that the effect lasts for at least 3 weeks.It transiently downregulates the expression of NaV 1.6 and NMDAR1 in the hippocampal CA3 region,at least in rats.These changes may be one of its anti-epileptic mechanisms.