Objective To investigate the correlated clinic factors of prostecdtive efficacy and recurrence in chronic hepatitis B patients who got the combinated fully respond to Lamivudine.Methods To divide the chronic hepatitis B patients who got the combinated fully respond to Lamivudine into two groups,according to whether the curative effect was consolidated after the withdrawal of lamivudine therapy for two years.Group A was consolidated and group B was repeated.To compare the two groups correlated clinic factors.Results Consolidated ratio of prostecdtive efficacy was 64.71% in the first year,then it dropped to 55.88% in the second year.There was apparent difference between two groups which included:ages,total months of treatment,serum HBV DNA before and after treatment,ALT,time to HBeAg seroconversion and consolidation.Particularly is time stoped a medicine,the pro-s 1 Ag was negative,the HbeAg fixed amount is a 0 Ncus/ml,two all match 33 example,only 1 while stopping a medicine for 12 months relapse,two years long-term curative effect consolidation rate reaches to 97%,.But come short of twos to all match 35 example,29 HBVDNA rebound,relapse of illness,two years long-term curative effect consolidation rate reaches to 17.14% only.(P=0.0000).Conclusions To achieve the withdrawal indication after long-term antiviral therapy with nucleoside according to The Guideline of Prevention and Treatment for Chronic Hepatitis B,it still must refer to that the pro-s1Ag was negative.whether the quantitated index of HbeAg had achieved to 0Ncu/ml,otherwise,it was necessary to extend the course of treatment of antiviral therapy.The extensive course of treatment was very safe,because the ratio of mutation of YMDD and some other virus was tiny.

This experiment was carried out in 65 New Zealand rabbits. The resultsrevealed that the febrile response and increaed PGE_2 level in cerebrospipel fluid (CSF) were significantly reduced when the calcium-channel-blocking agent (verapamil) wasintravenously injected prior to ET. But, verapamil had no marked effect on the increasedcAMP level in csf during ET-induced fever. It was suggested that most likely this anti-pyretic action was due to the effect on biosynthesis of PGE in hypothalamus, while,cAMP might not be involved in the mechanism of this antipyretic action.

Objective To evaluate the efficacy and safety of recombinant human tumor necrosis factor-α receptor Ⅱ IgG: Fc fusion protein for injection (rhTNFR: Fc) treatment in Chinese patients with early rheumatoid arthritis (ERA) and active spondyloarthritis. Methods This was a large-scale, multicenter, open-label, phase Ⅳclinical observational study. The dosage of rhTNFR: Fc was 50 mg per week, combined or not combined with other drugs. The primary endpoint of efficacy included the proportion of patients with low disease activity [simplified disease activity index (SDAI)≤11] at 3 month and 6 month. Secondary endpoint variables included clinical disease activity index (CDAI), disease activity score 28 based on C-reactive protein (DAS28-CRP) and health assessment questionnaire(HAQ). The primary endpoint of SpA was the ankylosing spondylitis disease activity score (ASDAS-CRP) at month 3. The secondary endpoints were the proportion of subjects achieving ASDAS<1.3 and ASDAS<2.1, Bath AS functional index (BASFI) and CRP. All subjects in the study were evaluated for safety. T test, χ2 test and rank sum test were used for satistical analysis. Results One thousand two hundred and seventy subjects with ERA were studied. The difference between the baseline and month 3 after treatment in SDAI, CDAI, DAS28-CRP were 26 ±16, 23 ±15, 1.86 ± 1.01 respectively (P<0.01). The above parameters 6 months after treatment were similar to those at 3 months after treatment, which demonstrated the persistence of drug efficacy. Two thousand three hundred and twenty eight subjects of SpA were studied. The difference between the baseline and 3 months after treatment in ASDAS, BASFI was 2.6 ±1.7, 3.4 ±3.8 respectively (t=73 .54, t=42 .36, P<0.01). The overall incidence of adverse events was 8.03%(289/3 598), including the common adverse events such as injection site reactions, skin rash and elevated liver enzyme levels. Adverse events were improved after proper treatment, without severe infections and tumor. These data confirmed that the overall safety of rhTNFR: Fc was good. Conclusion The study confirms that rhTNFR: Fc is effective for the treatment of ERA and SpA, and it is safe and well-tolerated.

Aim To study the chemical constituents of the underground part of Ligularia pleurocaulis (Franch.) Hand-Mazz.. Methods The dried roots and rhizomes of L.pleurocaulis were extracted with methanol. Isolation and purification were performed by silica gel column chromatography and recrystallization etc. Structures of the pure compounds were established on basis of spectral analysis. Results Twelve compounds were obtained from L.pleurocaulis, they were 6-angeloyloxy-furanoligu-larenone (1), 2-oxo-3-hydroxy-eremophila-1(10),3,7(11),8-tetraen-8,12-olide (2), tiglic acid (3), oleanolic acid (4), lupeol (5), β-sitosterol (6), daucosterol (7), caffeic acid (8), emodin (9), 7-methoxy-coumarin (10), ferulic acid (11) and 4-hydroxy-2,5-dimethoxy-benzaldehyde (12). Conclusion Compound 1 is a new eremophilane and compound 2 is a new natural compound. All above compounds were obtained for the first time from L.pleurocaulis.

[Objective]To investigate whether syrinx should by treated before correction in patients with scoliosis complicated by syringomyelia without neurologic impairment. [Method]Thirty-five cases were divided into experimental group(group A,without preoperative surgical intervention to syrinx,15 cases)and control group(group B,with preoperative surgical intervention,20 cases) randomly. The coronal plane Cobb's angle,the length of syrinx,the position of syrinx,the biggest diameter of syrinx and S/C ratio were measured and compared between 2 groups.[Result]There was no significant difference in the correction rate and loss rate between two groups.The postoperative syrinx index(biggest diameter of syrinx,syrinx length and S/C ratio) was significantly smaller than that of preoperation(P

Cholangiocarcinoma is rare. It is highly malignant and early diagnosis is difficult. Various risk facts have been linked to the carcinogenesis and development of cholangiocarcinoma. Chronic inflammation and cholestasis are the two common pathways in the malignant transformation of cholangial cells. We reviewed the molecular biological researches of cholangiocarcinoma from two aspects: its risk factors and related pathomechanism.

Objective To investigate the role of NF-KB binding element in regulation of NOD2. Methods Promoter region of NOD2 containing the NF-κB binding site was amplified by PCR from human genome DNA and correctly connected to the vector pEGFP-N3 which had been cut out promoter by restriction enzyme to obtain the GFP expression vector driven by human NOD2 gene promoter. The constructed plasmids were transiently transferred into cell line HeLa by LipofectAMINETM2000 and the GFP expression was ob- served by the inversion fluorescence microscope. The NF-κB binding site in the constructed vector pEGFP- N3-NOD2wt was deleted by the QuikChange site-directed mutagenesis kit. The recombinant plasmid mpEG- FP-N3-NOD2 was transiently transferred into cell line HeLa by LipofectAMINETM2000, and the GFP expres- sion was observed by the inversion fluorescence microscope. Results The constructed pEGFP-N3-NOD2wt plasmids and mpEGFP-N3-NOD2 were the same as the design confirmed by restriction digestion and se- quence analysis. The results of the cell transient transfection indicated that different strength of GEP ex- pressed by recombinant plasmids in HeLa cells could be observed. The GFP expression of constructed mpEGFP-N3-NOD2 was lower than that of pEGFP-N3-NOD2wt. Conclusion The GFP expression vector driven by human NOD2 gene promoter which contains the NF-κB binding site, and the site deleted plasmid were successfully constructed. The GFP expression of recombinant plasmid mpEGFP-N3-NOD2, deletion of the NF-KB binding site, was obviously weaken in HeLa. The results indicate that NF-KB binding element may play a positive role in regulation of NOD2 gene, which establishes favourable bases for further study on the mechanism of NOD2 gene expression and regulation.

AIM: To investigate the role of fibrocystin/polycystin (FPC) in autosomal recessive polycystic kidney disease (ARPKD) development by means of screening the protein interaction using yeast two-hybrid approach. METHODS: The constructed pGBKT7-FPC was used as the bait to screen the pre-transformed human fetal kidney cDNA expression library by yeast two-hybrid assay to obtain the host cell protein which interacted with C-terminal region of FPC. The sequence transformation screening experiment was applied to confirm the protein interactions in yeast. RESULTS: After yeast mating and co-transformation screening analysis, Klotho (KL) was selected from the host cells and the interaction between KL and FPC was further confirmed. CONCLUSION: C-terminal region of FPC can interact with KL, which probably provide the approach for further studying the role and biochemistry mechanism of FPC protein in ARPKD.

OBJECTIVE: To provide reference for reducing disadvantageous drug-drug interaction(DDI)and avoiding adverse drug event(ADE). METHODS: The patients aged more than 65 were selected from cardiovascular department in our hospital dur-ing Jun. 2015-Mar. 2016. The influential factors for potential DDI(PDDI)-induced ADE were analyzed. The relationship of related factors with PDDI-induced ADE was analyzed by multivariate Logistic regression analysis. RESULTS:A total of 328 patients were included,involving 257 PDDI patients,and totally 452 cases of PDDI (including 247 cases of mild PDDI,149 cases of general PDDI and 56 cases of severe PDDI). The age,the number of drugs used simultaneously,Ccr and liver function (Child-Pugh score)were related to the occurrence of PDDI-induced ADE(P<0.01). CONCLUSIONS:For PDDI-induced ADE,the risk evalua-tion can be conducted for a series of factors,including age,the number of drugs used simultaneously,Ccr and liver function. For high-risk patients,intervene should be conducted in advance to reduce the risk of ADE.

AIM:To investigate the role of fibrocystin/polycystin (FPC) in autosomal recessive polycystic kidney disease (ARPKD) development by means of screening the protein interaction using yeast two-hybrid approach. METHODS:The constructed pGBKT7-FPC was used as the bait to screen the pre-transformed human fetal kidney cDNA expression library by yeast two-hybrid assay to obtain the host cell protein which interacted with C-terminal region of FPC. The sequence transformation screening experiment was applied to confirm the protein interactions in yeast. RESULTS:After yeast mating and co-transformation screening analysis,Klotho (KL) was selected from the host cells and the interaction between KL and FPC was further confirmed. CONCLUSION:C-terminal region of FPC can interact with KL,which probably provide the approach for further studying the role and biochemistry mechanism of FPC protein in ARPKD.