1.Correlated Clinic Factors of Prostecdtive Efficacy and Recurrence in Chronic Hepatitis B Patients Who Got The Combinated Fully Respond to Lamivudine
Journal of Medical Research 2006;0(03):-
Objective To investigate the correlated clinic factors of prostecdtive efficacy and recurrence in chronic hepatitis B patients who got the combinated fully respond to Lamivudine.Methods To divide the chronic hepatitis B patients who got the combinated fully respond to Lamivudine into two groups,according to whether the curative effect was consolidated after the withdrawal of lamivudine therapy for two years.Group A was consolidated and group B was repeated.To compare the two groups correlated clinic factors.Results Consolidated ratio of prostecdtive efficacy was 64.71% in the first year,then it dropped to 55.88% in the second year.There was apparent difference between two groups which included:ages,total months of treatment,serum HBV DNA before and after treatment,ALT,time to HBeAg seroconversion and consolidation.Particularly is time stoped a medicine,the pro-s 1 Ag was negative,the HbeAg fixed amount is a 0 Ncus/ml,two all match 33 example,only 1 while stopping a medicine for 12 months relapse,two years long-term curative effect consolidation rate reaches to 97%,.But come short of twos to all match 35 example,29 HBVDNA rebound,relapse of illness,two years long-term curative effect consolidation rate reaches to 17.14% only.(P=0.0000).Conclusions To achieve the withdrawal indication after long-term antiviral therapy with nucleoside according to The Guideline of Prevention and Treatment for Chronic Hepatitis B,it still must refer to that the pro-s1Ag was negative.whether the quantitated index of HbeAg had achieved to 0Ncu/ml,otherwise,it was necessary to extend the course of treatment of antiviral therapy.The extensive course of treatment was very safe,because the ratio of mutation of YMDD and some other virus was tiny.
2.Application of nasolabial flaps in repair of defects after midfacial skin tumor removal
Jin XU ; Zhuming WU ; Yong YU ; Meifeng ZHANG ; Chaofeng ZHANG
Chinese Journal of Medical Aesthetics and Cosmetology 2015;21(6):328-330
Objective To investigate the methods and effects of nasolabial flaps for repair of defects after skin tumor removal on midface.Methods From Augest 2012 to March 2015,the different flaps from nasolabial fold were utilized to repair 22 skin defects due to surgical removal of skin tumors on midface.There were 12 flaps with subcutaneous pedicle and 10 flaps with skin pedicle in the group.The size of the defects ranged from0.5cm × 1.0cmto4.0cm × 5.0cm.Results After operation,all flaps survived with primary healing,cosmetic results were satisfactory with conceal scar and no facial organs distortion after 1 month to 2 years following-up.No tumor recurrence occurred.Conclusions Thenasolabial flaps with appropriate pedicles could obtain the satisfactory results for the reconstruction of medium-sized skin defects on midface,especially suitable for middle-aged and elderly patients.
3.Comparative study of diagnostic value of computed tomographic virtual endoscopy versus electronic colonoscopy for colon cancer in elderly patients
Junwen XU ; Chaofeng HOU ; Zuqi ZHAO ; Juan LI
Chinese Journal of Geriatrics 2016;35(9):964-967
Objective To study the diagnostic value of computed tomographic virtual endoscopy versus electronic colonoscopy for colon cancer in elderly patients.Methods The 69 cases of elderly patients with colon cancer received CTVE and electronic colonoscopy before surgery to compare achievement rates,sensitivities,and endoscopy coincidence rate with pathologic classification between the two methods.Results For a definite diagnosis of colon cancer in elderly patients,achievement ratio was 98.6% (68/69) in CTVE and 62.3% (43/69) in electronic colonoscopy(x2 =14.72,P< 0.05);sensitivity was 95.6% (65/68) in CTVE and 97.7% (42/43) in electronic eolonoscopy(x2=0.003,P > 0.05);endoscopy coincidence rate with pathologic classification was 95.4% (62/65) in C-TVE and 95.2%(40/42) in electronic colonoscopy.Conclusions CT virtual endoscopy may be one of examination methods in elderly patients with colon cancer.
4.Analysis of risk factors that affect type 2 diabetic arrhythmia
Peng DONG ; Jing XU ; Chunhong ZHANG ; Chaofeng LIU
Journal of Chinese Physician 2016;18(11):1655-1657,1661
Objective To investigate risk factors that affect type 2 diabetic cardiac arrhythmia.Methods Clinical data of 292 diabetic patients who were performed examination of low limb vascular were analyzed retrospectively.Based on electrocardiogram,the patients were divided into cardiac arrhythmia group and non-cardiac arrhythmia group with case-control study,and the clinical data and evaluation indexes of lower extremity vascular disease were compared between two groups.Results Data comparison between two groups indicated age [(59 ± 14) years vs (54 ± 12) years],symptoms of low limbs [40.7% vs 29.0%] had statistical significance (P < 0.05).Glycosylated hemoglobin,hypertension history,results of color Doppler ultrasound for lower limbs artery,ankle-brachial index,toe-brachial index,transcutaneous oxygen pressure,heat-controlled laser Doppler between two groups had no statistical significance (P > 0.05).Age,gender,history of high blood pressure,symptoms of low limbs,and heat-controlled laser Doppler were analyzed with multiple logistic regression,which indicated that age was risk factor of type 2 diabetic arrhythmia.Conclusions Age is correlated with type 2 diabetic arrhythmia,and evaluation indexes of lower limb vascular disease have no significant correlation with type 2 diabetic arrhythmia.
5.Effect of second-line antiretroviral treatment on human immunodeficiency virus/acquired immune deficiency syndrome in Zhengzhou
Zhaoyun CHEN ; Kongju WU ; Yan SUN ; Qingxia ZHAO ; Chaofeng LI ; Lixia XU
Chinese Journal of Infectious Diseases 2017;35(5):282-285
Objective To evaluate the effect of second-line antiretroviral treatment (ART) on human immunodeficiency virus/acquired immune deficiency syndrome (HIV/AIDS) and provide reference for subsequent HIV/AIDS treatment.Methods Two hundred and twenty-eight HIV/AIDS patients received second-line ART during January 2011 and December 2015 in Zhengzhou were included.Two hundred and forty-eight who received first-line ART during this period were randomly enrolled as control group.CD4+ T cell count and HIV RNA load before and after treatment were compared with x2 test and t test when appropriate.Results There were 228 patients (137 male and 91 female) in the second-line ART group and 248 patients (176 male and 72 female) in the control group.In second-line ART group, CD4+ T cells increased from (274±200)/μL to (476±261)/μL after an average treatment of (39.5±18.8) months.The difference was statistically significant (t=12.91, P<0.05).In control group, CD4+T cells increased from (251±199)/μL to (470±233)/μL after an average treatment of (35.7±14.7) months.The difference was statistically significant (t=14.60, P<0.05).CD4+ T cells showed no statistical difference between two groups regardless of treatment (t=1.25 and 0.26, respectively, both P>0.05).During the treatment, the rates of immunological failure were 9.6% (22/228) in second-line ART group and 12.9% (32/248) in the control group.There was no statistical difference between two groups (x2=1.251, P>0.05).Complete viral inhibition rates were 83.3% (190/228) in second-line ART group and 88.7% (220/248) in control group with no statistical difference (x2=2.881, P>0.05).Conclusions Second-line ART regimen has equivalent treatment efficacy with first-line ART.To achieve a better outcome, second-line ART regimen should be selected as an alternative option when first-line regimen fails.Compliance is the key to guarantee the success of antiviral therapy.
6.Analysis of Intestinal Microbiota of Type 2 Diabetes Patients of by Two Fingerprint Technologies
Xiaokang WU ; Chaofeng MA ; Pengbo YU ; Lei HAN ; Jiafeng YIN ; Ni ZHANG ; Miaoxian LI ; Xiangling WANG ; Jiru XU
Journal of Modern Laboratory Medicine 2015;(4):24-27
Objective To explore the characteristics of intestinal Microbiota in T2DM patients by two molecular fingerprint technologies,and investigate the correlation of intestinal microbiota and T2DM,and evaluate the application value of two fin-gerprint technologies.Methods Fecal samples of 8 healthy groups and 7 diabetes patients were collected.Then the total DNA of gut microbiota was extracted.Through the analysis of products by two molecular fingerprints of ERIC-PCR and DGGE-PCR,ecological characteristics of diversity and similarity of gut microbiota were obtained in healthy groups and dia-betes patients.Results Compared to healthy groups,the number of bands and Shannon-Wiener index of gut microbiota in di-abetes patients was decreased but no statistical significance.The similarity in patients group was declining(P <0.05),and the construction of gut microbiota was inclined to differ.Two fingerprint technologies of ERIC and DGGE could directly re-flect the diversity of gut microbiota and were the modern molecular biological techniques without depending on cultivation. ERIC was simple and convenient,had a better reflection of microbial diversity,but gel band cutting and regarded asa proper approach with higher diffraction efficiency and excellent repetition to studysequencing couldn’t be performed since there were more influencing factors on the experiment.DGGE could better reflect the ecological characteristics such as microbial diversity and similarity,and selecting bands,gel band cutting and sequencing could be done.Conclusion The composition and construction of gut microbiota in diabetes patients were changed,which suggests the occurrence of the disease had the correlation with gut microbiota.ERIC and DGGE is regarded as a proper approach with higher diffraction efficiency and ex-cellent repetition to study intestinal microbiota,but also gel band cutting,sequencing,bacteria identification can be performed by DGGE,both can be used in combination.
7.Preparation of a biphasic calcined bone of beta-TCP/HAP and its application in BMP-2 gene medicine.
Xiaoliang XU ; Tingting TANG ; Jueren LOU ; Chaofeng YU ; Kerong DAI
Journal of Biomedical Engineering 2003;20(4):630-633
In this study, we produced a biphasic absorbable calcined bone (CB) of beta-TCP/HAP, and evaluated its function as a carrier of bone marrow derived mesenchymal stem cells(BMSCs) in BMP-2 gene medicine. Biphasic CB was manufactured and its surface was coated by collagen. X-ray diffraction analysis, scanning electron microscopy and biomechanical measurement were performed on the product. Heterotopic bone induction of product as carrier of BMP-2 gene transferred BMSCs and its biodegradability were tested in nude mice and goats. X-ray diffraction analysis showed biphasic patterns of HAP and beta-TCP. Scanning electron microscopy showed the porosity were similar to those of the cancellous bone, and the adhesion of cells on the CB surface were better after surface-coating with collagen. It had certain biomechanical strength and appropriate biodegradability. Biphasic CB loaded with Adv-hBMP-2 transduced BMSCs could induce much bony callus at the subcutaneous site of nude mice and the tibial bone defects of goats. The results showed biphasic CB is a superior carrier of cells in BMP-2 gene medicine.
Animals
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Biodegradation, Environmental
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Bone Morphogenetic Protein 2
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Bone Morphogenetic Proteins
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genetics
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Bone Regeneration
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drug effects
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Bone Substitutes
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chemistry
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pharmacology
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Calcium Phosphates
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pharmacology
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Cell Differentiation
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Goats
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Hydroxyapatites
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pharmacology
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Materials Testing
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Mesenchymal Stromal Cells
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cytology
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Mice
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Mice, Nude
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Transfection
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Transforming Growth Factor beta
8.Clinical characteristics and risk factors of atrial fibrillation in elderly patients
Chaofeng HOU ; Junwen XU ; Fenglin SONG
Chinese Journal of Geriatrics 2020;39(8):870-873
Objective:To investigate the clinical characteristics and risk factors of paroxysmal atrial fibrillation(AF)and persistent atrial fibrillation in elderly patients, in order to provide a theoretical basis for clinical diagnosis and treatment and risk assessment of adverse prognosis.Methods:A total of 201 non-valvular AF patients aged 60-90 years admitted to Heji Hospital from Jan.2017 to Aug.2019 were enrolled in this retrospective study.Of the 201 patients, 102 cases met the diagnostic criteria for paroxysmal AF and 99 cases met the diagnostic criteria for persistent AF.During the same period, 100 healthy elderly people from the physical examination center were included.Clinical data, laboratory test results and echocardiography data were collected.Differences in clinical parameter values between the two groups were analyzed by Hotelling's Trace multivariate analysis.Risk factors for AF were analyzed by comparison of correlation factors, single factor analysis and unconditional logistic regression multivariate analysis.Results:The paroxysmal AF group had a mean age of (70.2±6.5) years old, with 73 males(73.7.6%)and 26 females(26.3%), while the persistent AF group’s mean age was (65.3±5.23), with 61 males(59.8%)and 41 females(40.2%). There were a significant difference in age between the paroxysmal AF group and the persistent AF group( t=5.99, χ2=4.39, P<0.05). Hotelling's Trace multivariate analysis indicated differences in clinical parameter values between the two groups( F=6.26, P<0.01). Levels of serum uric acid, homocysteine(Hcy), high sensitivity C-reactive protein(hs-CRP)and D-dimer, and anterior and posterior diameter of the left atrium were higher in the persistent AF group than in the paroxysmal AF group( P<0.05), while levels of total cholesterol, platelet count and left ventricular ejection fraction(LVEF)were higher in the paroxysmal AF group than in the persistent AF group( P<0.05). History of hypertension( OR=8.92, 95% CI: 4.18-19.05)and smoking history( OR=4.47, 95% CI: 1.87-10.71)were risk factors for persistent AF, while history of hypertension( OR=9.11, 95% CI: 4.21-19.69), smoking history( OR=3.56, 95% CI: 1.44-8.81)and drinking history( OR=9.32, 95% CI: 2.49-34.96)were risk factors for paroxysmal AF. Conclusions:The incidences of AF can be significantly reduced by controlling hypertension within an ideal range and quitting smoking and drinking.High concentrations of serum Hcy, D-dimer, hs-CRP and uric acid and increased anterior and posterior diameter of the left atrium may contribute to the persistence of AF.
9.Protective effects of Baibu Tang on bleomycin-induced pulmonary fibrosis in mice
Weina XIE ; DING DING ; Jing SUN ; Chaofeng ZHANG ; Mian ZHANG ; Xianghong XU
Journal of China Pharmaceutical University 2018;49(4):483-489
This study aimed to investigate the protective effects of Baibu Tang on bleomycin-induced pulmonary fibrosis in mice. After intratracheally giving bleomycin(3. 5 mg/kg), mice were orally administered Baibu Tang once a day for 14 consecutive days, takingnintedanib as a positive control. The anti-fibrotic effects were assessed by the hydroxyproline level and the histopathological changes in H&E or Masson stained lung tissues. The results revealed that the lung coefficient, hydroxyproline content, inflammation and collagen deposition were increased significantly in the lung tissue of the model mice. Both ethanol and water extracts of Baibu Tang significantly improved all the pathological indexes in mice, but the effect of the ethanol extract was better than that of the water extract. Baibu Tang with Baibu(root of Stemona tuberosa)containing different components(neotuberostemonine, tuberostemonine and stemoninine, respectively)could significantly reduce hydroxyproline level and collagen deposition in the lung tissue of bleomycin-induced mice, and there was no significant difference in their activity. This result showed that the changes in the chemical composition of Stemona tuberosa(Baibu, monarch drug for Baibu Tang)have little effect on the anti-fibrosis activity of Baibu Tang, and its mechanism and material basis need further investigation.
10.Protective effects of stemona alkaloids on mice with bleomycin-induced pulmonary fibrosis
Juan XIANG ; Ping YU ; Mingdan LI ; Chaofeng ZHANG ; Xianghong XU ; Mian ZHANG
Journal of China Pharmaceutical University 2017;48(1):76-81
This study aimed to investigate the protective effect of total alkaloids(TA)and one of the active components, neotuberostemonine, of Stemona tuberosa on bleomycin-induced pulmonary fibrosis in mice and to explore the mechanism by fibroblasts model. The bleomycin-induced mice were orally administered with TA(60 mg/kg)and neotuberostemonine(10, 20 mg/kg), with prednisone(6. 67 mg/kg)as a positive control. The anti-fibrotic effects were assessed by hydroxyproline content, TGF-β1 level, inflammatory score, collagen deposition and the expression of α-SMA in the lung tissues. The results revealed that TA and neotuberostemonine could significantly ameliorate the inflammation and injury, and attenuate the hydroxyproline content and collagen deposition. Moreover, neotuberostemonine treatment markedly down-regulated the α-SMA level and TGF-β1 content in bleomycin-injured mice lungs. The in vitro experiments showed that neotuberostemonine inhibited the expression of α-SMA induced by TGF-β1 in a dose-dependent manner, indicating that suppression on differentiation of fibroblasts to myofibroblasts may be one of the mechanisms for neotuberostemonine against pulmonary fibrosis.