Medical linear accelerator is precisely large-scale medical equipment with high integration and complex construction.In use,it is easy to occur fault,and the maintenance process is tedious,and the cost of maintenance is higher.The servicers with medical engineering technique should proficiently know and grasp the structure and principle of this kind of equipment,and respond routine fault,and ensure the quality of accelerator in clinical application.This article analyzed a case of interlocking fault of stand power supply(STPS)of 23EX medical linear accelerator in the department of medical engineering of the Affiliated People's Hospital of Ningbo University.According to the performance of accelerator's fault,and combining the work principle of occurring STPS interlocking fault,the pulse width modulation(PWM)board in auxiliary electronic cabinet of accelerator was located.Its schematic diagram was analyzed and checked.Finally,it was determined that the reason was the field effect tube of PWM board was broken down.The fault was eliminated after the components of occurring fault was replaced.We can increase maintenance efficiency and technical level,and reduce the cost of maintenance for hospital,and ensure its quality in clinical application through quickly locate and resolve faults.

Medical linear accelerator is precisely large-scale medical equipment with high integration and complex construction.In use,it is easy to occur fault,and the maintenance process is tedious,and the cost of maintenance is higher.The servicers with medical engineering technique should proficiently know and grasp the structure and principle of this kind of equipment,and respond routine fault,and ensure the quality of accelerator in clinical application.This article analyzed a case of interlocking fault of stand power supply(STPS)of 23EX medical linear accelerator in the department of medical engineering of the Affiliated People's Hospital of Ningbo University.According to the performance of accelerator's fault,and combining the work principle of occurring STPS interlocking fault,the pulse width modulation(PWM)board in auxiliary electronic cabinet of accelerator was located.Its schematic diagram was analyzed and checked.Finally,it was determined that the reason was the field effect tube of PWM board was broken down.The fault was eliminated after the components of occurring fault was replaced.We can increase maintenance efficiency and technical level,and reduce the cost of maintenance for hospital,and ensure its quality in clinical application through quickly locate and resolve faults.

Obesity usually exacerbates the immunosuppressive tumor microenvironment (ITME), hindering CD8⁺ T cell infiltration and function, which further represents a significant barrier to the efficacy of immunotherapy. Herein, a multifunctional liposomal system (CR-Lip) for encapsulating celastrol (CEL) was utilized to remodel obesity-related ITME and improve cancer immunotherapy, wherein Ginsenoside Rg3 (Rg3) was detected interspersed in the phospholipid bilayer and its glycosyl exposed on the surface of the liposome. CR-Lip had a relatively uniform size (116.5 nm), facilitating favorable tumor tissue accumulation through the interaction between Rg3 and glucose transporter 1 overexpressed in obese tumor cells. Upon reaching the tumor region, CR-Lip was found to induce the immunogenic cell death (ICD) of HFD tumor cells. Notably, the level of PHD3 in HFD tumor cells was effectively boosted by CR-Lip to effectively block metabolic reprogramming and increase the availability of major free fatty acids fuel sources. In vivo, experiments studies revealed that the easy-obtained nano platform stimulated enhanced the production of various cytokines in tumor tissues, DC maturation, CD8⁺ T-cell infiltration, and synergistic anticancer therapeutic potency with aPD-1 (tumor inhibition rate = 82.1%) towards obesity-related melanoma. Consequently, this study presented an efficacious approach to tumor immunotherapy in obese mice by encompassing tumor eradication, inducing ICD, and reprogramming metabolism. Furthermore, it offered a unique insight into a valuable attempt at the immunotherapy of obesity-associated related tumors.

Objective To construct an intelligent recognition,prevention,and control system for infectious disea-ses and multidrug-resistant organism infections,aiming at improving the efficacy of full-process management,and to evaluate its application effects.Methods Based on personalized logic parsing rules that accurately reflect the infec-tion status and transmission risks in real-time,an intelligent recognition,prevention,and control system with func-tions of automatic recognition,dynamic labeling,real-time sharing,early warning,and visual guidance was estab-lished.Patients undergoing invasive diagnostic and therapeutic procedures in two departments of a tertiary first-class hospital from October 2023 to May 2024 were selected as the research subjects.The differences in recognition,pre-vention,and control efficacy before and after the application of the system were compared using a self-controlled method,with traditional manual management as the control group and intelligent system management as the experi-mental group.Results A total of 2 146 patients were included in the analysis.The recognition,prevention,and control rate and the accuracy rate of recognizing infected individuals using the intelligent system were enhanced sig-nificantly compared with those using manual mode(improved from 5.3％and 72.4％to 100％,respectively),with statistical significance(both P＜0.001).The median early warning time for infection information reached 85.20 days,with 100％early warning achieved.The average time spent by medical staff on infection information recogni-tion and management was reduced by 4.71 hours per day.Conclusion The intelligent system constructed in this study significantly improves the effectiveness of full-process management in recognition,prevention,and control of infectious diseases and multidrug-resistant organism infection,effectively reduces the risk of cross-infection,and en-hances the efficiency of diagnostic and therapeutic services.

Objective To construct an intelligent recognition,prevention,and control system for infectious disea-ses and multidrug-resistant organism infections,aiming at improving the efficacy of full-process management,and to evaluate its application effects.Methods Based on personalized logic parsing rules that accurately reflect the infec-tion status and transmission risks in real-time,an intelligent recognition,prevention,and control system with func-tions of automatic recognition,dynamic labeling,real-time sharing,early warning,and visual guidance was estab-lished.Patients undergoing invasive diagnostic and therapeutic procedures in two departments of a tertiary first-class hospital from October 2023 to May 2024 were selected as the research subjects.The differences in recognition,pre-vention,and control efficacy before and after the application of the system were compared using a self-controlled method,with traditional manual management as the control group and intelligent system management as the experi-mental group.Results A total of 2 146 patients were included in the analysis.The recognition,prevention,and control rate and the accuracy rate of recognizing infected individuals using the intelligent system were enhanced sig-nificantly compared with those using manual mode(improved from 5.3％and 72.4％to 100％,respectively),with statistical significance(both P＜0.001).The median early warning time for infection information reached 85.20 days,with 100％early warning achieved.The average time spent by medical staff on infection information recogni-tion and management was reduced by 4.71 hours per day.Conclusion The intelligent system constructed in this study significantly improves the effectiveness of full-process management in recognition,prevention,and control of infectious diseases and multidrug-resistant organism infection,effectively reduces the risk of cross-infection,and en-hances the efficiency of diagnostic and therapeutic services.

Trauma-associated bone infection is one of the most serious infectious diseases after surgery, characterized by high incidence, complex pathogenic microorganisms, therapeutic difficulty and poor prognosis. Therefore, early accurate identification of pathogenic microorganisms and sensitivity of related antibiotics is very important to the success of surgery as well as of local and systemic antibiotic support therapies. As traditional detection methods have such shortcomings as complex items, numerous indicators, slow speed, a low positive rate, and inability to detect rare pathogenic microorganisms, there is an urgent need for better detection methods to identify pathogenic microorganisms of bone infection. In recent years, polymerase chain reaction (PCR) and metagenomic next generation sequencing (mNGS) have been used in clinical diagnosis of a variety of infectious diseases because of their advantages in accuracy, speed, efficiency, and detection range of pathogenic microorganisms over traditional cultures. PCR and mNGS can also be used as effective tools to diagnose trauma-associated bone infection. This review explores the advantages, disadvantages, development trends and guiding significance of these two techniques in the diagnosis of trauma-associated bone infection.

3D printing, an emerging technology, can assist improving surgical efficiency of liver surgery. 3D-printed organ models facilitate surgeons in surgical planning, enabling the simulation of surgical procedures and guiding interventions through surgical navigation. Furthermore, 3D printing harnesses the potential of utilizing tissue or cells from specific individuals to generate grafts for liver surgeries, thereby addressing the issue of graft shortage and advancing the development of personalized medicine.

Trauma-associated bone infection is one of the most serious infectious diseases after surgery, characterized by high incidence, complex pathogenic microorganisms, therapeutic difficulty and poor prognosis. Therefore, early accurate identification of pathogenic microorganisms and sensitivity of related antibiotics is very important to the success of surgery as well as of local and systemic antibiotic support therapies. As traditional detection methods have such shortcomings as complex items, numerous indicators, slow speed, a low positive rate, and inability to detect rare pathogenic microorganisms, there is an urgent need for better detection methods to identify pathogenic microorganisms of bone infection. In recent years, polymerase chain reaction (PCR) and metagenomic next generation sequencing (mNGS) have been used in clinical diagnosis of a variety of infectious diseases because of their advantages in accuracy, speed, efficiency, and detection range of pathogenic microorganisms over traditional cultures. PCR and mNGS can also be used as effective tools to diagnose trauma-associated bone infection. This review explores the advantages, disadvantages, development trends and guiding significance of these two techniques in the diagnosis of trauma-associated bone infection.

Abstract： Objective　To elucidate the antigenic antibody reaction of recombinant expression of non-structural protein 1 (NS1) of Japanese encephalitis (JE) virus with various mosquito-borne flaviviruses, including JE virus, and the antigenic antibody reaction of serum samples of patients infected with JE virus in acute stage. Methods　In this study, Escherichia coli prokaryotic expression vector (pET) system was used to recombinant express Japanese encephalitis virus NS1 gene. Western Blot assay was performed to detect the antibody responses of the recombinantly expressed protein against a variety of mosquito-transmitted flaviviruses, including JE virus, as well as antigen-antibody reactions of serum from patients with acute JE virus infection. Results　The NS1 gene expression product of JE virus (P3 strain) was in the form of an inclusion body, and the denatured and renatured expression product was displayed as a single band in the denatured gel (polyacrylamide gel electrophoresis, PAGE), with a molecular weight of about 45 000. The results of further antigen-antibody analysis showed that the antigen/antibody hybridization reaction of the expression product with polyclonal or monoclonal antibody of JE virus (mosquito isolates, encephalitis isolates) and serum samples of patients with acute JE virus infection could be completely consistent. The recombinant product showed negative antigen/antibody hybridization reactions with mosquito-transmitted flaviviruses, such as dengue virus and yellow fever virus polyclonal antibodies, but positive reactions with polyclonal antibodies to West Nile virus and Murray Valley encephalitis virus. Conclusions　In this study, the recombinant expression of the NS1 protein of JE virus was successfully obtained, and the antigen/antibody reaction between the recombinant protein and samples of patients infected with mosquito-borne flavivirus and JE virus was analyzed. The study results provide important basic data for elucidating the antigen-antibody reaction between the NS1 protein of JE virus and mosquito-borne flavivirus. The recombinant expression protein obtained in this study provides an important material basis for further research on the function of JE virus NS1 protein.

Gastric cancer is one of the common tumors in the world and a major cause of cancer death.Although the 5-year survival rate of gastric cancer patients has increased greatly with the improvement levels of diagnosis and treatment,the high malnutri-tion rate of gastric cancer patients still has a significant impact on their overall survival and quality of life.Malnutrition is considered an independent prognostic factor for cancer patients,early detection of malnutrition in gastric cancer patients and more reasonable peri-operative nutritional support play an important role in the survival and prognosis of gastric cancer patients.This article combines exist-ing research at domestic and abroad to review the nutritional risk screening and assessment of gastric cancer patients during periopera-tive period,as well as the research progress of perioperative nutritional support and immunonutrition,in order to provide more compre-hensive nutritional management strategies for patients with gastric cancer during the perioperative period.