0.05) after non-fever limit (non-FL) dose and FL dose endotoxin (ET) was intravenously injected into rabbits. The increase of PGE_2 in CSF was not limited during the occurrence of ET FL. 2. Intracerebroventricular injection (ICV) of different dose of PGE_2 into rabbits induced dose-dependent fever, but there was no more rise in body temperature when the febrile response had reached a certain height. This is termed "PGE FL". 3. The concentration of cyclic adenosine-3′, 5′-monophosphate (cAMP)in CSF paralleled the fluctuation of temperature (r=0.9906, P

40 New Zealand white rabbits were used to observe the effect on the fever caused by ET (endotoxin) and EGTA [ethylene glycol bis-(?-aminoethylethylether)-N, N, N′, N′-tetraacetic acid] after ICV (intracerebroventricular) infusion of Tau and CaCl_2 into rabbits. The results showed that Tau ICV perfusion could inhibit the initial febrile response to ET and EGTA in rabbits (P0.05), which could be blocked by ICV infusion of CaCl_2. The authors suggested that Tau might increase the level of Ca~(++) in the hypothalamus and reduce Na~+/Ca~(++) ratio, then lower the fever.

Objective To examine miRNA-31 expression in serum of children with airway allergic disease (asthma and allergic rhinitis) and determine the correlation with CD44.Methods The expression of miR-NA-31 and CD44 in serum of 26 children with acute asthma and allergic rhinitis and non-acute phase and 36 normal control children were determined by real-time reverse transcriptase polymerase chain reaction.Results The miRNA-31 was significantly overexpressed in serum of children with acute asthma and allergic rhinitis compared with non-acute phase and normal control children and significantly correlated with CD44.Conclusion The correlation between miRNA-31 and CD44, the aberrant expression of miRNA-31 may account for the occurrence and development of airway allergic diseases.

Objective To investigate neoplastic lesions in gastrointestinal tract treated by endoscop-ic mucosal resection (EMR). Methods The properties, extent and infiltrating depth of the neoplastic lesions in gastrointestinal tract were detected by chromoendoscopy, magnifying videoscopy and endoscopic ul-trasonograpy. The tumors in niucosa or its muscular layer including the early cancer, precancerous lesion, submucosal tumors, polyps in laterally development, large polyps with or without peduncle were removed by EMR or transparent plastic cap EMR. Results Thirty-one submucosal tumors and four early cancers (esoph-ageal cancer 2, cardiac carcinoma 1 , early colonic carcinoma 1 ) and moderate or severe gastric dysplasia 2 were treated by EMR or transparent plastic cap EMR, no recurrence happened within 3-18 months follow-up. Laterally spreaded 13 polyps and 21 subpedunculated or sessile typed large polyps were resected by the procedures of EMR and Endoscopic Piecemeal Mucosal Resection ( EPMR). Five (7. 04% ) cases were complicated with bleeding during the procedure, and cured under endoscopies. One patient with villus adenoma ( 1. 41% ) recurred three months after EMR. Conclusion Under the guidance of chromoendoscopy, magnifying videoscope and endoscopic ultrasoundgraphy, EMR is a safe and effective endoscopic therapy for early cancers, submucosal tumors and large polyps in gastrointestinal tract.

Objective To evaluate the efficacy and safety of sodium aescinate for abdominal operation in general surgery.Methods A multicenter,prospective and open labeled trail was conducted.Data of 2001 patients in PPS group who had undergone either conventional treated (n =500) or sodium aescinate treated (n =1 501) for abdominal operation in general surgery were analyzed.In the sodium aescinate treated group,sodium aescinate was given by ivgtt 12 h before the surgery started,and 20 mg daily after the surgery for 6 days.Time to recovery of gastrointestinal sounds (TRGS),time to recovery of passage of gas (TRPG),and time to recovery of bowel movements (TRBM) were recorded to evaluate the bowl function recovery.The difference of incision edema was observed by the evaluate edema level.Results The TRGS of sodium aescinate treated group was significantly shorter than that of the conventional treated group,P ＜ 0.01.The TRPG of sodium aescinate treated group was significantly shorter than that of the conventional treated group,P ＜ 0.01.The TRBM of sodium aescinate treated group was significantly shorter than that of the conventional treated group,P ＜ 0.01.The incision edema of sodium aescinate treated group was better than that of conventional treated group.The rate of adverse event was low in the sodium aescinate treated group,phlebitis was the most common one,accounting for 0.66％.No serious adverse event happened.Conclusion Sodium aescinate is effective and safe in accelerating patients recovery in general surgery.

AIM: To identify and quantitative analysis gentiopicroside content in the extract of Violent gentian of Tibetan herbal medicine. METHODS: Application of HPLC-the electricity sprays fog-mass spectroscopy/mass spectroscopy(HPLC-ESI-MS/MS) and the technique of LC-DAD-UV,by contrast with the reservation time of the authentic gentiopicroside,the ultraviolet absorbed spectrum,the molecule~+,quickly identified the gentiopicroside in the extract of violent gentian.As to it's quantitative analyis we adopted the capillary HPLC methods,with the weight of authentic gentiopicroside for abscissa. RESULTS: MS showed that the methanol extract of violet gentian mainly had five peaks.the compound structure of first peak was gentiopicroside,molecule~+ 356.9,result showed that gentiopicroside in gentian was 1.97%. CONCLUSION: Gentiopicroside is the mainly component of violet Gentia.

AIM: To investigate association between children interleukin 1?(-511) gene polymorphisms and susceptibility to febrile seizures. METHODS: Children were devided into two groups: those with febrile seizures (FS) and healthy control, FS group was also devided into family history group and non-family history group. Single nucleotide polymorphisms of IL-1?-511 gene in promoters were detected by PCR-RFLP among these groups. RESULTS: Genotypes for IL-1? promoter -511 (T-T, T-C, C-C) in total febrile seizures and healthy controls were not significantly differen. Genotype IL-1?-511(T-T) in family history patients is 46.2% ,which is much higher than those in non-family history group (10.3%) and healthy control group (5.7%). The genotype proportions for IL-1? promoter-511 in family history group were markedly different from those in non-family history patient group and control group (P

Objective To describe the vaccine inoculation rate of hemorrhagic fever with renal syndrome (HFRS) and the movement characteristics of rural population in the Xi'an epidemic area.Methods This was a cross-sectional study.The study covered all the four HFRS traditional high incidence areas (counties):Zhouzhi,Huxian,Chang'an and Lintong.Three villages were selected in each county and 20 households were selected in each village in 2013.Participants included all the persons of these families.People 16-60 years old were interviewed face to face about their sociodemographic and environmental information,and at the same time related HFRS vaccine history and characters of migrant workers were collected.Results Nine hundred and ninety-six persons were investigated,69.3 percent (690/996) were vaccinate-age population.Of all,49.0 percent (338/690) had HFRS vaccine history and 53.9 percent (372/690) had worked away from home most of the year.The inoculation proportion of workers which worked in their own counties (56.8％,67/118) were higher than that of workers worked outside (37.0％,60/162;39.1％,36/92,x2 =10.74,6.44,all P ＜ 0.017).In the workers that went back home every week,60.3 percent (76/126) had vaccinated.Workers that went back home once a year had the lowest proportion of inoculation (16.7％,7/42).The morbidity of HFRS decreased significantly accompany with ascend of inoculation rate (Y =-0.524X + 38.319,t =-4.581,P ＜ 0.05).Conclusions After the free vaccinations,there is still a gap between the vaccine inoculation rate and prevention benefit.The current vaccination strategies need to be adjusted.

Objective To study the diagnostic value of computed tomographic virtual endoscopy versus electronic colonoscopy for colon cancer in elderly patients.Methods The 69 cases of elderly patients with colon cancer received CTVE and electronic colonoscopy before surgery to compare achievement rates,sensitivities,and endoscopy coincidence rate with pathologic classification between the two methods.Results For a definite diagnosis of colon cancer in elderly patients,achievement ratio was 98.6％ (68/69) in CTVE and 62.3％ (43/69) in electronic colonoscopy(x2 =14.72,P＜ 0.05);sensitivity was 95.6％ (65/68) in CTVE and 97.7％ (42/43) in electronic eolonoscopy(x2=0.003,P ＞ 0.05);endoscopy coincidence rate with pathologic classification was 95.4％ (62/65) in C-TVE and 95.2％(40/42) in electronic colonoscopy.Conclusions CT virtual endoscopy may be one of examination methods in elderly patients with colon cancer.

Objective To study the effect of BiPAP NIV in treating severe asthma that resists incretion.Method 38 asthma patients that resist incretion were selected with 19 patients given BiPAP NIV and the other 19 patients given oxyen by nasal catheter.We study the changes of blood gas analysis and days of hospitalization.Result After 4 days' treating,two group patients' PO_2 increase obviously and their dyspnea alleviate.In BiPAP NIV group,PO_2 is higher than that of the control group(P