1.Integrating radiology and histology via co-attention deep learning for predicting progression-free survival in patients with metastatic prostate cancer.
Yuanshen ZHAO ; Feng LIU ; Chaofan ZHU ; Chongzhe YAN ; Bangkang FU ; Junjie HE ; Xin XIE ; Rongpin WANG ; Zhicheng LI
Chinese Medical Journal 2025;138(22):3013-3015
2.Preparation of monoclonal antibodies with high specificity for glycated hemoglobin and establishment of a rapid detection method.
Chaofan YIN ; Yingfu ZHANG ; Kebei WANG ; Jichuang WANG ; Yongwei LI ; Xiaojun WANG ; Xudong WANG ; Panpan SHI ; Yunlong WANG
Chinese Journal of Biotechnology 2025;41(8):3165-3177
Hemoglobin A1c (HbA1c) has a unique structure that makes monoclonal antibody (mAb) preparation challenging. This study aims to develop a method for preparing HbA1c mAbs and establish a fluorescent immunochromatographic assay (FICA) for rapid detection of HbA1c. Three glycosylated peptides were synthesized and used to prepare complete antigens, which were identified by dot enzyme-linked immunosorbent assay (Dot-ELISA) and ultraviolet absorption spectroscopy. The complete antigens and natural HbA1c were used for cross-immunization of mice, and the optimal complete antigen was selected. The mouse with the highest serum titer was chosen for mAb preparation. The purity and specificity of the mAbs were verified, and a FICA method was developed. The optimal complete antigen, with a titer of 1:512 000, was successfully prepared and selected. Fusion with splenocytes resulted in four specific HbA1c antibodies (purity > 90%). The best antibody exhibited a binding constant (Ka) of 1.67×1010 L/mol with the antigen. Based on this antibody, a FICA method was successfully established, capable of producing results within 15 min. The method demonstrated a good linear range (3%-13% HbA1c, y=0.071 3x+0.005 6, R2=0.993 7), recovery rates of 98%-102%, precision < 10.00%, and no nonspecific reactions. Clinical testing of 210 samples showed positive agreement of 96.36%, negative agreement of 97.00%, and overall agreement of 96.68%. The receiver operating characteristic (ROC) curve analysis yielded an area under curve (AUC) of 0.980 9 [95% confidence interval (CI): 0.961 0-1.000 0], with high consistency verified in multicenter studies. We successfully developed a key technique for preparing HbA1c monoclonal antibodies and established a FICA method for rapid detection of HbA1c. It will provide an efficient and convenient detection method for the early diagnosis and long-term management of diabetes and its complications.
Antibodies, Monoclonal/biosynthesis*
;
Animals
;
Mice
;
Glycated Hemoglobin/immunology*
;
Mice, Inbred BALB C
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Humans
;
Antibody Specificity
;
Chromatography, Affinity/methods*
;
Enzyme-Linked Immunosorbent Assay/methods*
;
Female
3.Health Resources Supporting High-quality Development of Urban Industrial Economy:Situation Analysis and Countermeasure Suggestions
Qi LI ; Lu LI ; Huiwen LI ; Chaofan LI ; Shuping WANG
Chinese Health Economics 2024;43(7):47-51
Industry serves as the main body and driving force of national economy.The high quality of the urban industrial economy depends on the role of health resources in maintaining the health of the workforce population.It analyzes the allocation of health resources in typical cities ranked within the top 10 in terms of industrial added value,identifies prevailing the new situation facing the allocation of health resources,and puts forward suggestions.It is found that health resources supporting high-quality development of urban industrial economy is currently facing the main situations of population increase and decrease differentiation within the city,more frequent cross-regional medical treatment,and accelerated aging trend of labor force.
4.Health Resources Supporting High-quality Development of Urban Industrial Economy:Situation Analysis and Countermeasure Suggestions
Qi LI ; Lu LI ; Huiwen LI ; Chaofan LI ; Shuping WANG
Chinese Health Economics 2024;43(7):47-51
Industry serves as the main body and driving force of national economy.The high quality of the urban industrial economy depends on the role of health resources in maintaining the health of the workforce population.It analyzes the allocation of health resources in typical cities ranked within the top 10 in terms of industrial added value,identifies prevailing the new situation facing the allocation of health resources,and puts forward suggestions.It is found that health resources supporting high-quality development of urban industrial economy is currently facing the main situations of population increase and decrease differentiation within the city,more frequent cross-regional medical treatment,and accelerated aging trend of labor force.
5.Health Resources Supporting High-quality Development of Urban Industrial Economy:Situation Analysis and Countermeasure Suggestions
Qi LI ; Lu LI ; Huiwen LI ; Chaofan LI ; Shuping WANG
Chinese Health Economics 2024;43(7):47-51
Industry serves as the main body and driving force of national economy.The high quality of the urban industrial economy depends on the role of health resources in maintaining the health of the workforce population.It analyzes the allocation of health resources in typical cities ranked within the top 10 in terms of industrial added value,identifies prevailing the new situation facing the allocation of health resources,and puts forward suggestions.It is found that health resources supporting high-quality development of urban industrial economy is currently facing the main situations of population increase and decrease differentiation within the city,more frequent cross-regional medical treatment,and accelerated aging trend of labor force.
6.Health Resources Supporting High-quality Development of Urban Industrial Economy:Situation Analysis and Countermeasure Suggestions
Qi LI ; Lu LI ; Huiwen LI ; Chaofan LI ; Shuping WANG
Chinese Health Economics 2024;43(7):47-51
Industry serves as the main body and driving force of national economy.The high quality of the urban industrial economy depends on the role of health resources in maintaining the health of the workforce population.It analyzes the allocation of health resources in typical cities ranked within the top 10 in terms of industrial added value,identifies prevailing the new situation facing the allocation of health resources,and puts forward suggestions.It is found that health resources supporting high-quality development of urban industrial economy is currently facing the main situations of population increase and decrease differentiation within the city,more frequent cross-regional medical treatment,and accelerated aging trend of labor force.
7.Comparative Analysis of "Same Disease, Same Price" Policy in Diagnosis Related Group Payment Under the Goal of Tiered Healthcare Delivery
Chaofan LI ; Conghui LIU ; Mingyang SUN ; Lin WU
Medical Journal of Peking Union Medical College Hospital 2024;15(5):1045-1051
To compare and analyze the "same disease, same price" policy in the regionsimplementing diagnosis related group(DRG) payment reform, and to provide recommendations for further policy optimization and extension. DRG policies published on official website of healthcare security bureaus from all provinces and 190 cities between 1 January 2019 to 31 May 2024 were collected. Microsoft excel was applied to collect the information, and the comparative analysis method was used to summarize and analyze the DRG polices from three dimensions: region, healthcare facility, and disease groups coverage. The regions where the "same disease, same price" policy in DRG payment was implemented were limited and unevenly distributed. The policy has been implemented in 76 cities across 16 provinces, 2 municipalities, and 3 autonomous regions, accounting for 40% of the cities implementing DRG payment. The pilot cities were mainly concentrated in eastern China. Coverage of healthcare facilities varied across regions. This policy covered all levels of healthcare institutions in most of the pilot cities, while it was primarily implemented in secondary and tertiary hospitals in some regions. The selection criteria for primary disease groups were generally consistent: the common diseases with mature diagnostic, treatment techniques and stable medical costs. However, the number, relative weight, and distribution of primary disease groups varied across regions, with the number ranging from 3 to 117 and relative weight ranging from 0.16 to 1.57. The primary diseases were mainly concentrated in internal diseases, with asthma and hypertension being the most common disease groups. It is recommended to expand the scope of region coverage, determine the scope of hospital coverage according to local capacities, expand the scope of disease groups by stages, and evaluate the effect of policy implementation.
8.Health Resources Supporting High-quality Development of Urban Industrial Economy:Situation Analysis and Countermeasure Suggestions
Qi LI ; Lu LI ; Huiwen LI ; Chaofan LI ; Shuping WANG
Chinese Health Economics 2024;43(7):47-51
Industry serves as the main body and driving force of national economy.The high quality of the urban industrial economy depends on the role of health resources in maintaining the health of the workforce population.It analyzes the allocation of health resources in typical cities ranked within the top 10 in terms of industrial added value,identifies prevailing the new situation facing the allocation of health resources,and puts forward suggestions.It is found that health resources supporting high-quality development of urban industrial economy is currently facing the main situations of population increase and decrease differentiation within the city,more frequent cross-regional medical treatment,and accelerated aging trend of labor force.
9.Health Resources Supporting High-quality Development of Urban Industrial Economy:Situation Analysis and Countermeasure Suggestions
Qi LI ; Lu LI ; Huiwen LI ; Chaofan LI ; Shuping WANG
Chinese Health Economics 2024;43(7):47-51
Industry serves as the main body and driving force of national economy.The high quality of the urban industrial economy depends on the role of health resources in maintaining the health of the workforce population.It analyzes the allocation of health resources in typical cities ranked within the top 10 in terms of industrial added value,identifies prevailing the new situation facing the allocation of health resources,and puts forward suggestions.It is found that health resources supporting high-quality development of urban industrial economy is currently facing the main situations of population increase and decrease differentiation within the city,more frequent cross-regional medical treatment,and accelerated aging trend of labor force.
10.Health Resources Supporting High-quality Development of Urban Industrial Economy:Situation Analysis and Countermeasure Suggestions
Qi LI ; Lu LI ; Huiwen LI ; Chaofan LI ; Shuping WANG
Chinese Health Economics 2024;43(7):47-51
Industry serves as the main body and driving force of national economy.The high quality of the urban industrial economy depends on the role of health resources in maintaining the health of the workforce population.It analyzes the allocation of health resources in typical cities ranked within the top 10 in terms of industrial added value,identifies prevailing the new situation facing the allocation of health resources,and puts forward suggestions.It is found that health resources supporting high-quality development of urban industrial economy is currently facing the main situations of population increase and decrease differentiation within the city,more frequent cross-regional medical treatment,and accelerated aging trend of labor force.
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