2.Role of circulating microRNA-22 in pathogenesis of idiopathic pulmonary arterial hypertension
Chao ZHENG ; Qun LIU ; Zhengrong HUANG ; Rong TANG ; Wuyang ZHANG ; Ziguan ZHANG ; Delong LI ; Zhipeng ZENG
Chinese Journal of Emergency Medicine 2012;21(1):57-60
Objective To investigate the value of plasma miR-22 in diagnosis of idiopathic pulmonary arterial hypertension ( IPAH ),and its role of regulation mechanisms in the pathogenesis of the disease.Methods Circulating miR-22 levels of IPAH patients and healthy controls were evaluated by RTPCR.The silico analysis of targets for miR-22 was taken, and followed by eGFP reporter assay for verification of predicted target gene Myc binding protein (MYCBP). Results Compared with healthy controls,the expression of plasma miR-22 in IPAH patients was significantly decreased (P < 0.01 ).The area under curve (AUC) of ROC curve was 0.744.MYCBP was a real target of miR-22 confirmed by silico analysis and eGFP reporter assay. Conclusions The expression of plasma miR-22 was significantly decreased in IPAH patients,and it could serve as a potential biomarker for diagnosis.The miR-22 might be involved in the pathogenesis of the disease through promoting its target gene MYCBP to activate the c-Myc pathway.
3.The clinical application of procalcitonin, leukocyte count and C-reactive protein in elderly patients with infection
Pei WU ; Chun SHAN ; Qun ZHANG ; Yinfang XU ; Yanping QING ; Chao GAO ; Ruixia SU
Chinese Journal of Geriatrics 2012;31(10):833-836
Objective To analyze and compare the clinical application values of procalcitonin (PCT),leukocyte count (WBC) and C-reactive protein(CRP) in elder patients with infection.Methods In patients(age≥ 65 yrs,axillary temperature >38.0℃)with infection or suspected infection,PCT,WBC,CRP and other bacteriological examination were performed.The electronic medical records from the HIS system of our hospital were analyzed retrospectively in combination with medical history.Results Of the enrolled 219 patients,65 ones were in control group,48 ones SIRS,51 ones sepsis and 55 ones MODS.There was a positive correlation between the level of serum PCT and the infection degree.The Spearman correlation coefficient was 0.706 (95%CI:0.616-0.797,P=0.000).Based on the highest Youden index (sensitivity+specificity-1),the best cutoff point of diagnosis for PCT was >0.341 μg/L (sensitivity 84.5%,specificity 55.8%),a analysis of receiver operating characteristic(ROC) curve about PCT,WBC and CRP was carried.Area under the curve (AUC) of PCT to controlled infection was 0.916 (95%CI:0.864-0.967,P=0.000).Based on the highest Youden index (sensitivity+ specificity-1),the judging threshold of PCT to infection controlled or not was 0.73 μg/L (sensitivity 84.6%,specificity 88.0%).PCT level after treatment >0.73 μg/L showed the uncontrolled infection,< 0.73 μg/L controlled.Conclusions PCT has a higher specificity for elder patients with infection.The variation of PCT level can guide the application of antibiotics,avoid abuse and decrease the occurrence of drug-resistant bacteria.
4.Therapeutic effect of collagen from Cyanea nozakii on adjuvant arthritis in rats
Wen-Tao ZHANG ; Lu-Hong TANG ; Wei CHEN ; Dong-Qun CHEN ; Chao DENG ;
Chinese Journal of Marine Drugs 1994;0(04):-
Objective To investigate the suppressive effects of collagen from Cyanea nozakii on adjuvant induced arthritis inrat.Methods Rats with adjuvant arthritis received different do- ses of Cyanea nozakii collagen by intragastric administration for two weeks.Incidence and severity of arthritis were assessed by calculation of mean arthritis index,the concentrations of NO,MDA and activity of SOD in the serum were examined.Results Cyanea nozakii colla- gen at different doses could ameliorate the adjuvant induced arthritis,suppress the concen- trations of NO,MDA and increase the activity of SOD in the serum.Conclusion Cyanea noza- kii collagen has therapeutic efficacy in treatment of adjuvant arthritis rats,and the mecha- nism of Cyanea nozakii collagen is probably related to the antioxidation.
5.Diagnostic features of pathological fractures located in extremities caused by bone tumor or tumor like lesion
Yan-cheng LIU ; Yong-cheng HU ; Qun XIA ; Bao-shan XU ; Ning Jl ; Hong-chao HUANG ; Zhong-li ZHANG
Chinese Journal of Orthopaedics 2011;31(9):944-948
ObjectiveTo explore the clinical characteristics of pathological fracture in extremities caused by bone tumors or tumor-like lesions. MethodsFrom August 2002 to December 2010, 139 patients with pathological fractures were entered in the study, including 79 males and 60 females with an average age of 31.1 years. Fractures included tumor-like lesion in 55 cases, benign tumor in 13, giant cell tumor (GCT)in 7, primary malignant tumors in 28, and metastatic tumors in 36. Forces induced to fractures were classified into four grades: spontaneous fracture, functional activity, minor injury, severe injury. Age, fracture location, histological results, fractures forces, prodromes, and misdiagnosis were all observed. Chi-square test were use to compare forces and prodromes within different tumors. ResultsThe highest morbidity rate is 32.4%(45/139) which lies in 11-20 years old. The cites of fractures including femurs in 71 cases, humerus in 36, tibia in 15, fingers in 7, radiuses in 4, fibula in 3, ulnas in 2, and metatarsus in 1. Fracture forces include spontaneous fractures in 29 cases, functional activity in 42, minor injuries in 65, and traumatic injuries in 3. Sixty-seven patients(48.2%) had local prodromes. The prodromes of both malignant tumors and metastatic tumors were more than benign tumors. Twenty cases experienced misdiagnosis with average delay time of 12 weeks. ConclusionMinor injury forces and local prodromes are clinical key features of pathological fractures. Both of them are key points of avoiding misdiagnosis.
6.The clinical application of plantar medial perforator artery based reverse island medial dorsal pedal neurocutaneous vascular flaps.
Li-Qun CUI ; Jin-Gang CHEN ; Ming ZHANG ; Wan-Chao ZHAO ; Rui MA
Chinese Journal of Plastic Surgery 2009;25(3):178-180
OBJECTIVETo explore the clinical effect of plantar medial perforator artery based reverse island medial dorsal pedal neurocutaneous vascular flaps.
METHODS12 cases with soft tissue defects of forefeet were treated by plantar medial perforator artery based reverse island medial dorsal pedal neurocutaneous vascular flaps. The flap size ranged from 3.0 cm x 3.5 cm to 5.5 cm x 8.5 cm.
RESULTAll flaps survived completely. The patients were followed up for 6 - 24 months. The texture and flexibility of the flaps were normal with no ulcer. The sensation improved with the two-point discrimination of 7 - 10 mm. The cosmetic and functional results were satisfactory. The wounds at donor site healed primarily.
CONCLUSIONSThe flaps have expanded size for large defects with good flexibility, thickness and texture. It is easily performed with less morbidity to main artery.
Adult ; Female ; Foot Injuries ; surgery ; Humans ; Male ; Middle Aged ; Skin Transplantation ; Soft Tissue Injuries ; surgery ; Surgical Flaps ; blood supply ; innervation ; Tibial Arteries ; surgery ; Young Adult
7.Experimental study on the reconstruction of mandibular defects with sinboneHT bone replacement in rabbits.
Wei-qun ZHANG ; Yi-ren WANG ; Yong-lie CHAO
West China Journal of Stomatology 2010;28(2):124-127
OBJECTIVEAfter sinboneHT bone replacement (SBR) was implanted in animals, to evaluate the biocompatibility of SBR and compounded in autogenetic bone in the proportion of one to one in order to prepare for the clinical applications in the future.
METHODSBone defects of 10 mm x l0 mm x 2 mm was made at the mandibular of rabbits, then SBR with different granule diameter and autogenetic bone was compounded in the proportion of being applied in the left defects, while autogenetic bone was implanted in the right defects and nothing was used in the right reformed defects. Animals were sacrificed at 2, 4 and 8 weeks respectively. The biologic capacity was evaluated with anatomy, X-rays studies and histology.
RESULTSSBR has better biocompatibility, which can effectively accelerate the reconstruction of bone defects and help the new bone by being compounded with autogenetic bone. It provides the appropriate scaffold or template which would allow cellular infiltration, attachment and multiplication.
CONCLUSIONSBR is a kind of bone substitute material with good biocompatibility. SBR compounded with self-bone has a better regeneration function.
Animals ; Bone Regeneration ; Bone Substitutes ; Mandible ; Rabbits ; Reconstructive Surgical Procedures
8.Study on the preparation of TK-gene nanoparticles and its expression.
Qin HE ; Zhi-rong ZHANG ; Ji LIU ; Chao-qun XU
Acta Pharmaceutica Sinica 2004;39(4):285-287
AIMTo prepare TK-gene nanoparticles and investigate its expression.
METHODSBiodegradable and biocompatible polymer polylactic-co-glycolic acid (PLGA) was used to prepare recombinant plasmid pEGFP-AFP nanoparticles by double-emulsion evaporation technique. The characteristics of the nanopticicles including morphology, entrapment efficiency was investigated. The expression of TK gene was also investigated by MTT assay, which could determine the dying cells after the addition of gancyclovir (GCV). The enhanced green fluorescent protein (EGFP) expression in human hepatocarcinoma SMMC-7221 cells and human normal parenchymal Chang liver cells were assessed by flow cytometric analysis.
RESULTSThe resulting plasmid-nanoparticles had regular spherical surface and a narrow particle size with a mean diameter of (72 +/- 12) nm, The average entrapment efficiency was 91.25%, the enhanced transfection efficiency and ability protecting plasmid DNA from degraded by nuclease or sonication due to nanoparticles encapsulation.
CONCLUSIONDNA-nanoparticles need further study as gene delivery system.
Biocompatible Materials ; Carcinoma, Hepatocellular ; metabolism ; Cell Line, Tumor ; Drug Delivery Systems ; Ganciclovir ; pharmacology ; Genes, Reporter ; Green Fluorescent Proteins ; Herpesvirus 1, Human ; enzymology ; Humans ; Lactic Acid ; Liver ; cytology ; metabolism ; Liver Neoplasms ; metabolism ; Luminescent Proteins ; genetics ; metabolism ; Nanotechnology ; Particle Size ; Plasmids ; Polyglycolic Acid ; Polymers ; Recombinant Proteins ; genetics ; Thymidine Kinase ; genetics ; metabolism ; Transfection ; alpha-Fetoproteins ; genetics ; metabolism
10.Treatment of Thoracic Spine and Spinal Cord Injury
Zhi-cheng ZHANG ; Tian-sheng SUN ; Chao-qun YE ; Dajiang REN ; Zhi LIU ; Fang LI ; Shuqing LIU ; Shaoting XU
Chinese Journal of Rehabilitation Theory and Practice 2006;12(4):336-338
ObjectiveTo analysis the clinical features of thoracic spine and spinal cord injury (SCI) and summarize the inclusive standard of cellular transplant clinical trial for SCI.MethodsThe data of 72 cases with thoracic spine and spinal cord injury from 1990 to 2005 were analyzed retrospectively.ResultsMean follow-up period was 20 months (6~48 months). There was no recovery in 12 spinal cord injury without radiographic abnormality (SCIWORA) patients, but improvement of urine function in 4 cases. 5 cases of 52 fracture-dislocation complete injury were improved to grade B (sense recovery), rate of recovery was 9.6%; recovery rate was 62.5% in incomplete injury. Sense recovery of all cases was better than motor recovery. Partial cases appeared spasm paralysis relief.ConclusionIncidence rate of complete injury is high and recovery is bad in thoracic spine and spinal cord injury. The inclusive standard of cellular transplant clinical trial for SCI is old complete thoracic spinal cord injury without residual compression.