Objective To investigate the value of soluble epithelial cadherin(sE-cad)in the differential diagnosis of pleural effusion. Methods Patients were divided into malignant pleural effusion group,infective pleural effusion group and transudation group.sE-cad in pleural fluids obtained during the first thoracocentesis was measured by enzyme-linked immunosorbent assay(ELISA).The concentration of sE-cad in all kinds of pleural effusions was compared.The cut-off value of sE-cad for the differential diagnosis of benign and malignant pleural effusion was determined by ROC curve.The diagnostic value of sE-cad was also compared with common tumor markers such as CEA,CA199,CA125 and NSE.Results The concentration of sE-cad was significant higher in the malignant pleural effusion than in the benign pleural effusion[(38.38?4.15)ng/mL vs(14.17?0.80)ng/mL,P

OBJECTIVETo establish a ion chromatography method for determination of phosphorus oxychloride in the air of workplace.

METHODThe phosphorus oxychloride in the air of workplace was collected by absorb liquid and turned into hydrochloric acid, then separated in column and detected with conductivity detector, qualified by elution time and quantified by peak height or peak area.

RESULTSThe linear range of phosphorus oxychloride in air of workplace was 0.72 ∼ 5.76 µg/ml with its correlation coefficient 0.9999. The detecting limit of the method was 0.12 µg/ml. The smallest detecting concentration of the method was 0.08 mg/m(3) for 15 L sampling air. Relative standard deviation was 3.3% ∼ 6.2% and the recovery was 97.8% ∼ 103.8%. The sample could be resaved at room temperature at least for seven days.

CONCLUSIONThe indicators of the method correspond GBZ/T 210.4-2008«Guide for establishing occupational health standards-Part 4: Determination methods of air chemicals in workplace». It is a good method to determine phosphorus oxychloride in the air of workplace.

To study the mechanism of injection of sinomenine (Sinomenine,SIN)into Jiaji point in the treatment of L1-L5 segment Compression of fracture spine (VCF) in rabbits.Methods:54 rabbits were randomly divided into blank control group,model group and sinomenine group (group SIN)by the random number table.All the 2 groups established L1-L5 segment VCF model except the blank control group.After molding,the SIN group was injected SIN at both sides of the L1-L5 section of the vertebral column,the model group and the blank control group were injected the same volume of physiological saline as comparison.The 3 groups were treated for 4 weeks.Fracture of vertebral bone growth differentiation factor 2 (Growth differentiation,FACTOR2,GDF2),vascular endothelial growth factor (Vascular endothelial,growth factor,VEGF) and the expression of VEGF-mRNA were determined by immunohistochemical staining method;in venous blood,activities of nitric oxide (Nitric oxide NO),super oxide dissuade (Super oxide dims,unties,SOD) and bone protection Su (OPG) were detected by ELISA;immunofluorescence was used to measure two chitosan,image analysis method was used to detect spinal bone density in L1-L5 segment,the ELISA was used to detect inflammatory factor interleukin 1 beta (IL-1 beta),IL-6 beta,beta IL-17 in the spinal disc.Results:compared with the model group,in the SIN group,VEGF,VEGFmRNA and GDF2 in L1-L5 segment of spine were highly expressed,and the bone density increased (P＜0.05);two chitosan in intervertebral disc increased,IL-1beta,IL-6 beta,IL-17 beta,(P＜ 0.05);NO in venous blood decreased OPG,SOD increased (P＜0.05),the differences were statistically significant.Conclusion:injection of SIN into Jiaji can promote the healing of VCF in L1-L5 segment in rabbits.

Objective To investigate the efficacy and safety of catheter radiofrequency ablation of paroxysmal supraventricular tachycardia.Methods From Jul 2003 to Jan 2011,1106 cases with narrow QRS complex tachycardia who were treated by catheter radiofrequency ablation were recruited from our center and followed up for the rates of successful treatment,rcurrence and complications.Results There were in total 1106 patients (atrioventricular reentrant tachycardia:588 ; atrioventricular nodal reentrant tachycardia:477; atrial tachycardia:41 ),with a sex proportion of 1∶1.Successful ablation rate was 98.3 ％ (1087/1106).Of the 1087 successful ablation cases,43 (3.9％ )were warranted repeated ablation.The recurrent rates for trioventricular nodal reentrant tachycardia,atrioventricular reentrant tachycardia,left accessory pathway and right accessory pathway were 1.5％,5.6％,3.9％,and 9.1％ respectively.Complication rate was 1.5％.The major complications included pheumothorax ( 6 cases ),pulmonary embolism ( 1 case ),transient third-degree atrioventricular block ( 2 cases ),first-degree atrioventricular block ( 3 cases ),and persistent third-degree atrioventricular block(2 cases)with pacemaker implantation.There was one case of cardiogenic sudden death 5 days after the treatment procedure.The cause of his death was chronic stroke-related but not related to the operation procedure.Conclusion Catheter ablation has high efficacy and low complication rate in long-term follow-up,and is a promising treatment for paroxysmal supraventricular tachycardia.

Brain Edema ; etiology ; Brain Stem ; pathology ; Encephalitis ; etiology ; Female ; Hand, Foot and Mouth Disease ; complications ; therapy ; Humans ; Infant, Newborn

OBJECTIVETo establish a gas chromatographic method for detecting 2-butoxy ethanol in the air of workplaces.

METHODSAfter the air samples were collected with activated carbon tubes and desorbed with methylene chloride/methanol, the target toxicant was separated with FFAP capillary columns and detected with flame ionization detector, qualified by retention time and quantified by peak area.

RESULTSThe linear range of 2-butoxy ethanol in air of workplace was 56.3 ∼ 901.0 µg/ml, the correlation coefficient was 09999. The limit of detection was 2.0 µg/ml. The limit of quantity was 5.0 µg/ml. The minimal detecting concentration was 0.27 mg/m(3) in the condition of 7.5L sampling volume and 1ml desorbed volume. Relative standard deviation was 3.04% ∼ 7.93% and the recovery was 92.7% ∼ 95.5%.

CONCLUSIONIn present study the detecting method with high sensitivity, precision and accuracy can be used to determine 2-butoxy ethanol in the air of workplaces.

The uhrastructure and atrial natriuretic peptide(ANP)mRNA in myocardium of streptozotocin-induced diabetic rats were studied along with the effect of rosiglitazone.It was found that the myocardial ultrastructure was demaged in diabetic rats.Rosiglitazone may ameliorate the lesion in myocardium,and lower the ANP mRNA in diabetic rats(1.14?0.05 at 6 weeks and 1.12?0.09 at 10 weeks vs 0.97?0.14,both P

The expression of the vgb gene in vivo could improve the fermentation density and then contribute the extracellular secretion of the product of bxn gene. Constructed the recombination plasmid pPIC9K-vgbbxn and transformed into Pichia pastoris GS115. The results of PCR and SDS-PAGE indicate that the vgb gene and bxn gene had integrated into the genome of Pichia pastoris GS115 and expressed in efficient level. Also, the protein activity of their products had been verified respectively. Shake flask fermentation experiments showed that the presence of VHb in yeast Pichia pastoris efficiently enhanced cell growth and secretive expression of bxn gene under hypoxic habitats.
Aminohydrolases ; genetics ; metabolism ; Bacterial Proteins ; genetics ; physiology ; Electrophoresis, Polyacrylamide Gel ; Pichia ; genetics ; Plasmids ; Polymerase Chain Reaction ; Recombinant Proteins ; biosynthesis ; Truncated Hemoglobins ; genetics ; physiology

To purify recombinant human nucleoside diphosphate kinase A (rhNDPK-A) efficiently in pilot scale, cells of rhNDPK-A producing E. coli were homogenized by high pressure under 4 degrees C, 950 Pa. The insoluble debris was removed by microfiltration and the soluble portion was concentrated by ultrafiltration. The resulted crude sample was loaded on DEAE-sepharose Fast Flow. The target fraction was collected and then load on Cibacron Blue 3GA Sepharose CL-4B. Eluted with buffer containing ATP from the AC column, rhNDPK-A was polished with ultrafiltration. The results showed that after homogenized 2 rounds, 1500g cells of E. coli brought crude sample containing 47.6g NDPK-A. Treated with microfiltration and ultrafiltration, 27.3g of NDPK-A were recovered from this bacteria homogenate. After 2-step purification with column chromatography and then polished with ultrafiltration, 17.2 g rhNDPK-A were collected with purity of 96.3%. The recovery of the whole purification process was 36.2%, and the productivity of rhNDPK-A was 1.15 g per 100 g wet cells. Comparing the recovery of each purification step, it was found that the recovery of polish is higher than that of affinity chromatography, which is higher than that of ion exchange chromatography. The limit step was the process of sample pretreatment among the 4 purification steps. Combine with the fermentation results reported before, it was deduced that the productivity of rhNDPK-A was 510 mg/L. In conclusion, an easily controlled purification condition with high yield provides material for the translation researches of NDPK; In addition, it was suggested the crucial step determine the recovery of non-secretive recombinant proteins might be the process of sample pretreatment, not be the process of column chromatography.
Chromatography, Affinity ; Chromatography, Ion Exchange ; Electrophoresis, Polyacrylamide Gel ; Escherichia coli ; genetics ; Humans ; NM23 Nucleoside Diphosphate Kinases ; genetics ; metabolism ; Pilot Projects ; Recombinant Proteins ; isolation & purification ; metabolism ; Ultrafiltration

Objective To investigate the effect ofplatelet-derived growth factor (PDGF) and conditional medium of U87 glioma cells on the migration ability of human mesenchymal stem cells (hMSCs) to understand the possible role of PDGF in the directional migration of hMSCs toward gliomas. Methods hMSCs were isolated from the whole bone marrow by adherent culture, and the expression of PDGF receptor (PDGFR-α, β) in the cells was examined by RT-PCR. In vitro migration assay was performed using transwell inserts to observe the effect of PDGF (0, 5, 50, and 125 ng/mL) and the conditional medium on the directional migration ability ofhMSCs. The changes in the migration ability of hMSCs in response to addition of anti-PDGF antibody in the conditional medium were investigated. Results RT-PCR detected the expression of PDGFR- αand PDGFR-β mRNA in the isolated hMSCs. In the cell migration assay, both PDGF and the conditional medium induced directional migration of hMSCs (P<0.05), which was significantly suppressed by anti-PDGF antibody P<0.05). Conclusion With chemokine-like activities, PDGF concentration-dependently enhances the directional migration of hMSCs toward gliomas in vitro.