Objective:To investigate the association of IL-18 gene promoter SNPs with susceptibility to TB and its protein production in Chongqing Han children. Methods:Polymorphisms at -137 and -607 locus of IL-18 gene promoter in 113 TB child patients and 167 healthy controls were detected by SSP-PCR. The IL-18 productions in cultured supernatants of PBMCs from 46 healthy children were measured by ELISA. Results:The frequency of IL-18 -137GG genotype were significantly higher in TB patients(81.4%) than that in controls(67.7%)(P=0.01),while the frequencies of -137GC genotype(1.8% vs 3.6%)and -137C allele(10.2% vs 18.0%) were conversely lower(P

Objective:To investigate the clinical efficacy and safety of Reduning injections in the treatment of infantile rotavirus enteritis. Methods:Totally 102 cases of infantile rotavirus enteritis in our hospital from June 2012 to June 2013 were randomly divided into two groups, the control group had 50 patients, and the treatment group had 52 cases. Both groups were given conventional symp-tomatic treatment, the control group was given 10 mg·kg-1 ribavirin injections in 100ml glucose injections for intravenous infusion, qd, and the treatment group was given 0. 5 ml·kg-1 Reduning injections in 100ml glucose injections for intravenous infusion, qd. Ev-ery 3-day was a course of treatment. The clinical curative effect of the two groups was observed, and the clinical symptoms, hospitaliza-tion time and adverse reactions were also studied. Results:After the treatment, the total effective rate in the control group was 70. 0%, while that in the treatment group was 92. 3%, and there was significant difference between the two groups (P<0. 05). The clinical symptom disappearance time and hospitalization time in the treatment group were significantly lower than those in the control group with statistically significant difference (P<0. 05). During the treatment, the control group had 5 cases of leucopenia and 2 cases of skin rash, and the patients all recovered after withdrawal of the drug, while the treatment group showed no obvious adverse reaction. Con-clusion:The curative effect of Reduning injections in the treatment of infantile rotavirus enteritis is promising, which can effectively improve the clinical symptoms, shorten the course of disease and reduce adverse reactions with the value of clinical application.

OBJECTIVE:To explore the anti-inflammatory effect of Musk eye drops on acute ceratitis.METHODS:The model of acute ceratitis resulting from surgical injury was established in rabbits and which were randomly assigned to Musk eye drops groups(0.3%,0.15%,and 0.05%),0.5% Cortisone acetete eye drops group and 0.9% Normal Saline group(control).The corneal epithelium growth at 24,48h,and 72h was observed by fluorescein(2%)staining,levels of protein,Ca2+,IL-1? in the aqueous humor were determined,corneas were weighted and corneas tissues were sampled for histopathologic examination.The anti-inflammatory effect of Musk eye drops on acute ceratitis resulting from surgical injury was discussed.RESULTS:Corneal bedewing was mitigated,transmigration of neutrophilic leukocytes were inhibited,levels of protein and IL-1? in the aqueous humor were decreased,level of Ca2+ in the aqueous humor was altered and the corneal epithelium growth was promoted in Musk eye drops groups(0.3%,0.15%,and 0.05%).CONCLUSION:Musk eye drops had remarkable anti-inflammatory effect on acute ceratistis.

Objective]To investigate the characteristics of Qingyingtang group side, learning worthy dialectical medication characteristics, to guide clinical practice. [Methods] Retrieve ancient and modern literature, combined with a comprehensive analysis of drug-induced flavor theory;drug potency and taste of Qingyingtang were demolished square analysis, summary described the taste Qingyingtang prescription characteristics. [Results] Analyse Qingyingtang prescriptions from the taste characteristics of compatibility aspects, it can be summarized as follows:salty Hanku vent, Qingreliangxue, Gan Han Zi camp, nourish lung and stomach, bitter cold stasis, blood stasis and new students, Xin Liang Qing Xuan, through heat transfer gas four aspects. Wu Jutong heat into the blood according to the clinical syndrome features, combined with the taste of the above diagnosis and treatment using the compatibility Herbal Prescription for TCM Syndrome methods election due to drug model, called the lead from future generations. [Conclusion] The taste of the theory of the clinical treatment combination prescription medication an important basis for Qingyingtang nature flavor compatibility demolition fomula research can enhance the the world of knowledge and understanding of compatibility, thereby improving clinical scholar ability prescription medication.

Cultivating innovative talents is a systemic project,which is influenced by educational ecosystem.The comparison with educational ecosystems of developed countries shows that there are lots of factors affect the cultivating innovative talents in China including the internal factors of education system as well as family and social factors.

High - risk corneal transplantation immunological rejection is a primary factor affecting the success or failure of the corneal transplantation. As a result, application of immunosuppressive agent in high - risk corneal transplantation rejection reaction occupies an irreplaceable position. However, many local and systemic adverse reactions appeared in the process of application. In order to improve the achievement ratio of the corneal transplantation, experts and scholars at home and abroad have obtained remarkable achievements in dosage form, route of administration and drug combination;meanwhile, a considerable progress was also made in genetic engineering. This paper is a review of prevention and treatment on high - risk corneal graft rejection reaction.

·Corneal neovascularization ( CNV) is one of the most important causes that affecting corneal transparency, and it is a high risk factor of allogeneic corneal graft rejection. lt has become a research focus for the regulation of CNV. microRNAs are a class of endogenous non-protein-coding micromolecule RNAs which play a critical role in regulating a series of life process.Researches in recent years show a close correlation between microRNA and CNV.ln this article we reviewed the recent advances in these researches.

?AlM: To discuss the differences in accommodative lag of different stimulation between the adolescent myopes who wore rigid gas permeable contact lens ( RGPCL ) and spectacles for one year.
?METHODS: Totally 85 myopes between 10-16y were enrolled in the study. Every subject was examined with the visual function test, medical optometry, corneal topography and ocular health check etc. of eyes. According to the above measurements and the compliance of children and their parents, 41 subjects were selected to wear RGPCL, 44 subjects were chosen to wear spectacles. Based on maximum plus to maximum visual acuity ( MPMVA) , accommodative lag was determined by dynamic retinoscopy method in two groups. The subjects were examined once every 3mo, accommodative lag was measured again using the same method for comparative analysis after one year. Wearing a year later, myopia progression of the two groups was measured and compared.
?RESULTS: There was no significant difference between two groups of initial accommodative lag (P>0. 05). After wearing one year, there was also no significant difference between the accommodative lag examined and the initial lag in RGPCL group (P>0. 05), the results demonstrate significant difference between the accommodative lag examined after wearing one year and the initial lag in spectacles group ( P < 0. 05 ). One year later, the differences were significant in accommodative lag between RGPCL and spectacles wearers stimulated by 2D, 3D and 4D ( P < 0. 05 ), and the accommodative lag increased with increasing stimulation in both groups. After wearing a year, the myopia progression of RGPCL group was less than spectacles wearers, and the differences were significant (P<0. 05).
?CONCLUSlON:After wearing RGPCL and spectacles one year in high accommodative stimulus, there is an obviously decreased in accommodative lag in RGPCL group, which suggests that RGPCL can control the progression of myopia in adolescents. The accommodative lag of the two groups increase obviously with increasing stimulation, which suggest that the adolescents need keeping good habits of reading.

Female ; Humans ; Adenomyosis/surgery* ; Endometriosis/diagnosis* ; Early Diagnosis ; Attention

Objective To evaluate the radiosensitization effect of low-temperature plasma on HepG2, A549, and HeLa cells.Methods Cells were divided into three groups, radiation group (R) , plasma treatment group(P), and plasma plus radiation group (P + R).After radiation, cell survival was detected by a cloning assay.Cell cycle distribution, apoptosis and ROS content were tested by flow cytometry.Western blot was used to measure the expressions of Caspase-3 and Bcl-2.Results Lowtemperature plasma showed radiosensitization effects on three different human malignant cell lines with a sensitivity enhancement ratio(SERD0) of 1.28,1.32 and 1.29.respectively.In these three different human malignant cell lines, compared with radiation alone group (R) , the G2/M arrest, apoptosis rate and ROS level in the group P + R were enhanced (the prolongation of G2/M arrest: t =9.52, 8.24, 9.53, P ＜ 0.05;the apoptosis rate: t =10.67, 38.56, 6.74, P ＜0.05;ROS content: t =9.41, 15.42, 13.53, P ＜0.05).In HepG2 cells and A549 cells, compared with group P, the prolongation of G2/M arrest, the apoptosis rate and ROS content of group P + R were enhanced (the prolongation of G2/M arrest: t =8.75, 20.37, P＜0.05;the apoptosis rate: t =8.43, 9.99, P ＜0.05;ROS content: t =4.82, 5.27, P ＜ 0.05).The expression level of Bcl-2 protein was downregulated in group P + R;by contrast, the expression level of Caspase-3 protein in group P + R was upregulated.Conclusions Low-temperature plasma can increase the radiosensitization of HepG2, A549 and HeLa cells with the enhancement of G2/M phase arrest, apoptosis induction and ROS generation.