Objective:To improve the work of drug quality sampling and testing. Methods:The regulation of testing related work in Drug Quality Sampling and Testing Regulation was analyzed and discussed. Results and Conclusion: Some suggestions were pro-vided in order to improve the regulation of testing work in Drug Quality Sampling and Testing Regulation.

Lymphatic vessels are important for the spread of solid tumors, and recent researches indicated that lymphangiogenic factors(VEGF C and VEGF D)could stimulate lymphangiogenesis in tumors by activating their receptors on the lymphatic endothelial cells, then enhance the incidence of lymph node metastasis. In addition, inhibition of VEGFR 3 signaling could suppress tumor lymphangiogenesis and metastasis to lymph nodes, thus, inhibition of lymphangiogenesis can be expected to be a new method of blocking tumor metastasis.

Objective To explore the reversion of astragaloside and NMDA receptor antagonist against the hyperphosphorylation of protein Tau induced by fetal intrauterine distress in neonatal rats.Methods The analysis of variance of factorial design was setted up two intervention factors which were fetal intrauterine distress (two levels:no disposition;a course of fetal intrauterine distress)and the drugs (three levels:iv Saline;iv astragaloside;iv MK -801).When the neonatal rats grew to 12weeks,the hippocampus was removed from the neonatal rats.Detected the content of glutamate in the hippocampus of rats by high performance liquid chromatography.The expression of protein Tau which includes p -AT8Ser202 and GSK -3β1H8 in the hippocampus of rats were detected by the methods of immunohistochemistry staining.Results Fetal intrauterine distress could significantly up -regulate the content of glutamate,which was not affected by MK -801,in the hippocampus of neonatal rats which was reduces by the astragaloside (P <0.05).And both influences presented subtracting effects (P <0.05).Fetal intrauterine distress and the drugs do not affect the total protein Tau in the hippocampus of rats (P <0.05).Fetal intrauterine distress could up -regulate the hyperphosphorylation of protein Tau in the hippocampus of neonatal rats which could be reduced by astragaloside and MK -801 (P <0.05).And the influences between fetal intrauterine distress and the drugs presented subtracting effects (P <0.05 ).Conclusion Our results indicate that fetal intrauterine distress reduce the hyperphosphorylation of protein Tau in neonatal rats though up -regulating the content of glutamate.GSK-3βis the key protein in this signaling pathway.

Side population cells (SPs) are selected by the efflux features of fluorescent dye Hoechst33342.SPs have the characteristics of cancer stem cells,and play an extremely important role in the occurrence and development of tumors.At present,the SPs in common types of malignant tumor have been studied extensively at home and abroad.The study of SPs may shed some light on the diagnosis and treatment of cancer.

Objective To investigate the application value of detection of serum miRNA-210,miRNA-23b in the patients with the femoral head necrosis.Methods The serum and clinical data of 135 patients with the internal fixation operation for the femoral neck fracture were collected.The patients were divided into the necrosis group (27 cases) and non-necrosis group (108 cases),used the real-time fluorescent quantitative PCR (Real time-PCR,RT-PCR) technology to detect serum miRNA-210,miRNA-23b expression in comparatively analysizing the relationship between the changes of serum miRNA 210,23b and occurrence of the femoral head necrosis.Results The serum microRNA-210,-23b expression in the necrosis group were 0.19±0.03,0.21±0.05,which in the non-necrosis group were 0.92±0.18 and 0.87±0.17.The serum microRNA-210,-23b expression in the necrosis group were significantly lower than those in the non-necrosis group,the difference had statistical significance (P＜0.01).The analysis results from the receiver-operating characteristic curve (ROC curve) showed that AUC of serum miRNA-210,miRNA-23b expression were 0.790 (95％CI:0.687～0.892,P=0.000) and 0.743 (95％CI:0.633～0.854,P=0.000),respectively.The sensitivity and specificity of miRNA-210 and miRNA-23b at the best threshold in diagnosis of the femoral head necrosis were 95.7％ and 93.9％,91.5％ and 92.3％,respectively.The analysis results showed that the serum miRNA-210,-23b expression had clinical significance in diagnosis of the femoral head necrosis.Conclusion Detection of serum miRNA-210,-23b expression can predict occurrence of the femoral head necrosis,estimate development condition of the desease.

This article introduced the rich resources of medicinal pteridophyte and its conditions of geographic distribution,morphologic and cultural characteristics.Through the investigation,resource collecting and protection test on the important medicinal pteridophyte in Guzhou,it discussed the conservation and utilization of medicinal pteridophyte medicinal pteridophyte in Guzhou,and provided evidence for resource protection of further reasonable exploitation.

Objective To investigate the safety,therapy and prophylactic effect of Omeprazole on stress ulcer in children with epidemic type B encephalitis.Methods Before and after medication,chest X-ray examination was performed.The result of occult blood(OB) was confirm by Colloidal gold assay in stool and/or gastric juice.Based on the result of OB,the patients were divided into therapy group[besides conventional therapy for encephalitis,Cimitidine group and Omeprazole group with positive result OB,was administered with 0.9% normal sodium 100 mL+Cimitidine 20-40 mg/(kg?d),iv,q12 h and normal sodium 100 mL+Omeprazol 0.5-0.8 mg/(kg?d),iv,qd,respectively] and prophylaxis group(Cimitidine group,Omeprazole group and control group,with negative result OB,were administered with same medicines as therapy group,respectively.Except control group being administered only 0.9%NS 100 mL,iv,(q12 h)).The effects of drugs on hemostasis,preventing hemorrhage,and the potential risk of acquired pneumonia result from drugs used were observed.Results In therapy group,the average time of hemostasis in Omeprazole group was obviously shorter than that of in Cimitidine group,there was significant difference between two groups(P0.05).Conclusions Both of the drugs is safety and effect to therapy or prophylaxis for the latent stress ulcer in short term.The effect of Omeprazole is better than those of Cimitidine.Using Cimitidine and Omeprazole,neither therapy nor prophylaxis for stress ulcer increaseds the potential risk of acquired pneumonia in children with epidemic type B encephalitis in this study.

Objective To investigate the effect of 18F-fluoro-deoxy-2-glucose hybrid positron emission tomography images combined CT image(I8F-FDG PET-CT) on gross target volumes in non-small-cell lung cancer (NSCLC). Methods Forty six patients with pathological defined NSCLC were included in the study. All patients were divided into three groups according to image features; lymphatic metastasis group(18 cases) , ate-lectasis group( 16 cases) , solitary focus group(12 cases). The gross target volumes by CT and by PET-CT (GTVct and GTVPET_CT)were compared with volume differentials and coherence. We also compared the coherence among the three groups. Results The median GTVCT and median GTVPET-CT of atelectasis group was 160.813 cm3 and 108.838 cm3(t = 13.592,P<0.05). The median GTVCT and median GTVPET-CT of lymphatic metastasis group was 65.233 cm3 and 84. 367cm3 (t = 2. 768, P < 0.05 ). In the solitary focus group , median GTVCT was 21.7 cm3,and median GTVPET-CT was 19.6 cm3 (t =0. 684,P >0.05). There was statistic difference in coherence among the three groups (lymphatic metastasis group 0.561, atelectasis group 0. 698, solitary focus group 0.740). Conclusion There are varying degrees of difference between GTVCT and GTVPET-CT in both volume differentials and coherence . Compared to CT, PET-CT could improve the accuracy of radio-therapy. Patients with lymphatic metastasis would most likely benefit from it.

Objective To investigate the risk factors of radiation-induced oral mucositis (ROM) and to provide evidence for the prevention of ROM. Methods The severity of ROM was observed and recorded in 56 patients with nasopharyngeal carcinoma. The association of the severity of mucositis with age , sex, clinical stage, concurrent chemoradiotherapy, white blood cell count,BMI index, smoking, alcohol intake, and history of diabetes were assessed by using multivariate logistic regression analysis. Results The severity of ROM was significantly associated with a decrease in leucocyte count (P<0.05), and it was not markedly correlated with the other eight factors (P>0.05). Conclusions Leukopenia was one of the risk factors for the development of ROM. In clinical pratice,leukopenia should be corrected in patients with bone marrow depression due to radiotherapy in a timely manner.

Improving the education quality of graduate students is the new situation of gradu-ate education reform and development to each university of China. During the overall process of ex-ploring and reforming quality control of graduate student talent cultivation, institute of life sciences in Chongqing Medical University put forward that intensively constructing the supervisors' conscious quality control of talent cultivation was the driving force to realize the quality improvement of talent cultivation. The construction and improvement of supervisors' conscious quality control on graduate education was reflected according to the supervisors' professional quality, postgraduates' thought moral education, and postgraduates' scientific literacy training, respectively. This will lay the foundation for the future comprehensive improvement of postgraduates' academic ethics and effective promotion of the educative reform on graduate education quality.