Objective To evaluate the incidence and degree of organic impairment after neonatal asphyxia,analyze the high risk factors of this and find a new approach to lower the mortality of asphyxia.Methods Two hundred and twenty-two newborns with asphyxia were studied. They were divided into mild asphyxia group and severe asphyxia group, and the function of main organs were classified as mild and severe, too. Chi-square tests were conducted for statistical analysis.Results The incidences of organic impairment and multi-organ impairment with asphyxia were 90.1 % and 71.6 % respectively. The frequency of organ dysfunction in severe asphyxia was significantly higher than that of mild group(P

Objective To study the therapeutical effect of glutathione(GL),a powerful antioxidant on the symptoms and ECG in patients with coronary heart disease and its mechanisms.Methods Eighty-five subjects with coronary heart disease were recruited(45 male and 40 female).The patients were randomized to receive GL (240 mg,ivgtt,qd,for 14 days,n=44)on the top of conventional treatment(aspirin+?-blocks+ACEI)or conven- tional treatement alone(control,n=41).The serum MDA,SOD,NO levels were determined.Electrocardio- graphy(ST stage,T wave)was examined.Results GL significantly improved clinical symptoms scores(2.0+0.5 vs control:1.5+0.5,P

Objective To explore the effect of nBiPAP on ET-1 of patients with overlap syndrome.Methods Levels of ET-1 in plasma of 25 cases of OS,20 cases of COPD were analysed by radioimmunoassay,and ET-1 in OS was measured before and after treatment.The relationship between ET-1 with SaO_2 during sleep was analyzed. Results (1)The ET-1 levels in plasma of OS group were significantly higher than those of COPD group before treatment(P

Purpose To eva1uate the effects of Annexin A3 on pro1iferation and apoptosis of gastric cancer ce11s. Methods The re-combinant p1asmid pYr-ads-4-Annexin A3 was constructed and ana1yzed by restriction ana1ysis and sequencing and was transfected into MGC803 ce11s. The stab1e transfectants were obtained after screening with G418. Western b1ot ana1ysis was used to examine the expres-sion of Annexin A3 before and after transfection. CCK8 assay,c1one assay and f1ow cytometry were used to study the effects of Annexin A3 on pro1iferation and apoptosis of MGC803 ce11s. Results The recombinant p1asmid pYr-ads-4-Annexin A3 was successfu11y con-structed. Western b1otting resu1ts indicated that the Annexin A3 expression was significant1y higher in ce11s transfected with pYr-ads-4-Annexin A3 compared with ce11s transfected with empty vectors and un-transfected ce11s( P<0. 05 ). CCK8 assay resu1ts showed the number of ce11s transfected with pYr-ads-4-Annexin A3 was significant1y higher than those transfected with empty vectors and un-trans-fected ce11s(P<0. 05). Moreover,the number of c1one in ce11s transfected with pYr-ads-4-Annexin A3 was significant1y higher than the other two groups(P<0. 05). Important1y,high Annexin A3 expression inhibited to apoptosis of MGC803 ce11s(P<0. 05). Con-clusion Annexin A3 expression p1ay important ro1es in tumorigenesis of gastric cancer. Annexin A3 cou1d promote the pro1iferation and inhibited apoptosis of gastric cancer ce11s and it might be a potentia1 target for gastric cancer treatment.

Objective To observe the effects of Huangqi Sanxian Decoction,an invigorating kidney and activating blood Chinese medicine,on ultramicrostructure of brain immunocyte of ovariectomized rats.Methods Fifty female rats were randomized into five groups:sham operated group,model group,high-dosage treatment group,low-dosage treatment group and Naofukang control group.The rats models in these groups were established by excising two ovary from dorsal incision,and the same size of fat was excised in the sham operated group.The treatment groups were administrated Huangqi Sanxian Decoction by gastric gavage,the period of treatment for 4 weeks.The status of hippocampal artrocytes,the level of interleukin-6 and serum estradiol were observed after treatment for 4 weeks.Results Ovariectomy can induce hyperplasia of hippocampal artrocytes in the central nervous system,while Huangqi Sanxian Decoction can reduce the artrocyte number,increase the estradiol level and decrease the interleukin-6 level in a dose-dependent manner.Naofukang control group showed no influence on estradiol level.Conclusion Huangqi Sanxian Decoction can inhibit the artrocyte hyperplasia and inflammatory responses in the hippocampas of central nervous system.It exerts protective effects by increasing the estradiol level probably.

Objective To investigate a potential role for ORF50 promoter of human herpesvirus 8(HHV 8)in reactivation of HHV 8 in primary effusion lymphoma(PEL)BC 3 cells by HIV 1 related inflammatory cytokine,hepatocyte growth factor/scatter factor(HGF/SF). Methods The persistent stimulation of BC 3 was performed by recombinant HGF/SF and treated BC 3 cells were collected at the 3 rd and 7 th day after persistent stimulation respectively. Northern blot, quantitative PCR(real time PCR), and electron microscopy(EM)were carried out to detect the expression of mRNA of minor capsid protein ORF26 and the presence of viral particles of HHV 8 from treated BC 3 cells. Co transfection of BC 3 and human umbilical veil endothelial cells (HUVECs) with HHV 8 ORF50 promoter driven luciferase construct built previously by us and recombinant expression plasmid named pEV containing HIV 1 Tat coding gene was performed and stimulation of co transfected cells with recombinant HGF/SF and/or HIV 1 recombinant gp120 was conducted at 24 hours after transfection to induce luciferase gene expression. Then relative luciferase activity unit(RLU) was calculated at 24 hours after stimulation. In the meantime, stimulation of co transfected cells with 12 O tetradecanoylphorbol 13 acetate(TPA) was performed to be used as positive control. Results It was found that HGF/SF induced an 4.1 folds increase in mRNA expression of ORF26 when it was added individually to BC 3 cells at the 7 th day after persistent stimulation. Viral particles of HHV 8 were readily identified in BC 3 cells stimulated with HGF/SF at the 7 th day with EM analysis. Recombinant HGF/SF could upregulate promoter activity of HHV 8 ORF50 promoter in BC 3 and HUVECs cells and however, HIV 1 Tat and gp120 failed to act synergistically with HGF/SF to enhance ORF50 promoter activity in HUVECs. Conclusions The results show that HIV 1 related inflammatory cytokine HGF/SF is responsible for the induction of HHV 8 lytic cycle replication and the induction may be, at least partly mediated by ORF50 promoter of HHV 8.

Cardiomyopathies ; congenital ; Heart Ventricles ; pathology ; Humans ; Infant, Newborn ; Male ; Myocardium ; pathology

Adult ; Chemotherapy, Adjuvant ; Diagnosis, Differential ; Female ; Humans ; Lung Neoplasms ; secondary ; Mediastinal Neoplasms ; diagnostic imaging ; drug therapy ; pathology ; surgery ; Mediastinum ; diagnostic imaging ; pathology ; Neoplasm Recurrence, Local ; Osteosarcoma ; diagnostic imaging ; drug therapy ; pathology ; secondary ; surgery ; Tomography, X-Ray Computed

Objective To develop a high-definition video display system for medical endoscope based on system on chip (SoC),and meet the requirement of high-resolution and real-time video display.Method A CMOS camera was used for video data capture.A SoC chip,integrated with a dual-core ARM Crotex-A9 processor and a field programmable gate array (FPGA),was employed as the kernel of the system.The HPS part of the SoC was used to build an embedded system to realize human-computer interaction,and the FPGA part was used to store and cache video data.The HPS and FPGA part were connected through a high-performance ARM AMBA AXI bus bridge broadband system,so as to achieve encoding of the cached video data and real-time display on screen.Results A high-definition video display system was built based on SoC.This system can achieve capture,processing and realtime display of high-definition video,as well as video freeze function.Conclusions The experimental results indicate that this system is feasible and effective,and possesses the advantages of customizability,multiple-exploitation and high performance of real-time video display.

BACKGROUND:Matrix metaloproteinase-1 can degrade extracelular matrix, which is mainly colagen type I, and has the potential to reverse fibrosis tissue. OBJECTIVE:To construct the recombinant adenovirus vector containing human matrix metaloproteinase-1 (hMMP-1) gene with GatewayTM Clone Technology, and observe the capacity of degrading colagen type IIIin vitro. METHODS: The gene hMMP-1 was amplified by using PCR from the pcDNA3.1 plasmid and was cut down by the double endonuclease. The linear gene fragment was connected to the entry vector pENTERTM 1A. Then the entry clone and the destination vectors pJTI? R4 Dest CMV-N-EmGFP pA Vector recombined using the LR reaction to form the expression clone pAd-hMMP-1-eGFP. The linear pAd-hMMP-1-eGFP cut down by endonucleasePac I was transfected into HEK293A cels to packaging the Ad-hMMP-1-eGFP. The transfected situation was observed under a fluorescence microscope, the target protein expression was detected by western-blot assay and RT-PCR. Cels can be divided into three groups: blank control group: HEK293A cels, AD-EGFP group: HEK293A cels were infected by Ad-eGFP, AD-HMMP1-EGF group: HEK293A cels were infected by Ad-hMMP1-eGFP and colagen type III. The content of colagen type III was detected by ELISA kits after 24, 48 and 72 hours. RESULTS AND CONCLUSION: It was confirmed that the entry vector and the destination vector both contained hMMP-1 target gene by restriction analysis and sequencing. The green fluorescent protein was observed in the 293A cels transfected by the Ad-hMMP-1-eGFP at 4 days. The fluorescence intensity was the highest at 10 days. The virus was colected at 12 days, the viral titer was determined as 4.84 × 1010 PFU/mL, the target protein was efficient expressionvia western-blot assay. Blank control group and AD-EGFP group had no obvious change of colagen content with the extension of time. The rate of colagen degradation in AD-HMMP1-EGFP group was 24%, 56% and 81% respectively at 24, 48, 72 hours. AD-HMMP1-EGFP group degraded colagen significantly compared with the other two groups (P < 0.01). The recombinant adenovirus vector containing hMMP-1 was successfuly constructed by using the Gateway technology, this method was more efficient and specific than with the traditional methods. The hMMP1 degraded colagen type III significantlyin vitro.