Objective To participate in the recognition of medical laboratory and to evaluate the performance of the instruction, we assess the performance of ABI 7300 real-time fluorescent quantitative PCR instrument.Methods According to United States of America Clinical and Laboratory Standards Institute(CLSI),assess the precision,accuracy,sensitivity and linearity of HBV-DNA detection result in ABI 7300 fluorescence quantitative PCR instrument.Results The Intraassay coefficient of variation is 2.38%-4.61%,and interassay coefficient of variation was 3.87%-5.33%,both achieve the performance analysis standard of gene ampli-fication test project regulated by Medical Laboratory Quality and Competence Accreditation Criteria.Accuracy:the overall mean bias in 2011 Ministry of Health EQA results was 2.14%,which met the quality assessment requirements of the ministry of health. Functional sensitivity:the CV of 100 IU/mL detection limit concentration was close to 20%,which accords with the The limit of detection of the kit;Linear:the correlation coefficient r2 is 1.0,linear relationship met the requirements.Conclusion The perform-ance index of ABI 7300 real-time fluorescent quantitative PCR instrument is accuracy,and it can provide fast,accurate reports for clinical department.

Malignant biliary obstruction is caused by biliary tract malignant tumors or by extrinsic compression from pancreatic cancer, liver cancer, etc. Clinically, the disease is characterized by jaundice, skin itching, hepatic failure, etc., and the patients endure a poor quality of life with a short survival time. The key point for the treatment of malignant biliary obstruction lies in dredging biliary drainage channel , timely reducing jaundice and protecting liver function. This paper reviews the relevant medical literature , that have been published both at home and abroad in recent years, concerning percutaneous transhepatic endobiliary radiofrequency ablation by using HabibTM EndoHPB catheter to treat malignant biliary obstruction in order to clarify the fact that percutaneous transhepatic endobiliary radiofrequency ablation is a safe and feasible treatment and this technique can effectively reduce the incidence of re-stenosis of implanted biliary metallic stent.

Objective:To put forward some suggestions by comparing the current situation in both private and public hospitals. Methods:Comparing the basic information, scale, human resource and medical service management situation between the private and public hospitals by analyzing the 2014 Beijing health authorities report. Results:There are 355 private hospitals, 76. 1% of them are not rated and 59. 7% are not appointed for the medical insur-ance, and 82. 8% need to rent the land. Although the number of private hospitals is more than that of the public ones, their actual beds and health resources respectively account for only 20. 2% and 16. 4%. Besides, their in-come, assets and services also account for only about 10%. Conclusions:There is a big gap between the private and public hospitals in terms of scale, human resources and service ability. The Government should implement the policy about the ratings, medical insurance and the use of land which is given to support the private hospitals, and speed up the diversified medical model.

Objective To explore the application of RNA interference(RNAi) in colorectal cancer gene therapy.Methods The related literatures in recent years were reviewed.Results RNAi causes a high effective and distinctive degradation of mRNA homologous in sequence to the dsRNA.This new technology has been successfully applied to research the genesis and the growth of colorectal cancer.Conclusion RNAi has been a new focus in gene therapy for colorectal cancer.

Biotech is currently the fastest growing sector in drug industry. Globally biotech products generated revenues of $55bn in 2004, in a total drug market worth $550bn. The USA is the primary market of biotech products, being the source of approximately half of sales in 2004. About 55% of biopharmaceuticals’ sales in 2004 came from the five leading products (Erythropoietin,Interferon,Human insulin,G-CSF,Rituximab).Great progress has been made in the biotech market of China over the last few years. About 60%~70% of recombinant protein biopharmaceuticals are expressed in mammalian cells in USA and EU, while most recombinant protein biopharmaceuticals are expressed in E.coli in China. Most biopharmaceuticals expressed in mammalian cell can’t be manufactured in China. The biotech industry comprise numerous, mostly small organizations. These organizations typically employ less than 100 people. Most biotech companies have never brought a product to market before they disappear. Therefore, it is very important for biotech companies to make an appropriate development strategy.

3cm than in the patients with the largerest dominant diameter≤3cm.Embolization with combination of PLE and GSP had improved the percentage of reduc- tion in myomas volume.Conclusion The factors affecting results include tumor size and location,using of GSP,and performing of bilateral embolization.

High - risk corneal transplantation immunological rejection is a primary factor affecting the success or failure of the corneal transplantation. As a result, application of immunosuppressive agent in high - risk corneal transplantation rejection reaction occupies an irreplaceable position. However, many local and systemic adverse reactions appeared in the process of application. In order to improve the achievement ratio of the corneal transplantation, experts and scholars at home and abroad have obtained remarkable achievements in dosage form, route of administration and drug combination;meanwhile, a considerable progress was also made in genetic engineering. This paper is a review of prevention and treatment on high - risk corneal graft rejection reaction.

Objective To investigate the effects of selective cyclooxygenase-2(COX-2) inhibitor nimesulide on the proliferation of colon adenocarcinoma cells in vitro and the expression of matrix metalloproteinase-2(MMP-2).Methods The human colon cancer cell lines HT-29 and HCT-116 were employed in the study,grouped as nimesulide group,DMSO control group and blank control group.After treatment with nimesulide,the inhibitory effect of nimesulide on the proliferation of cancer cells was quantified by MTT assay,and the expression of MMP-2 in the cells was detected by quantitative zymography.Results Nimesulide inhibited the proliferation of HT-29 and HCT-116 cells in time and dose-dependent manners.The inhibitory effect on HT-29 cells was stronger than that on HCT-116 cells.Nimesulide down-regulated the MMP-2 expression in HT-29 cells,whereas the expression in HCT-116 cells remained unchanged.Conclusion Nimesulide can obviously inhibit the growth of colon cancer HT-29 cells with positive COX-2 protein,suggesting that nimesulide may down-regulate the expression of MMP-2 by inhibiting the activity of COX-2.

External therapy of traditional Chinese medicine(TCM) is a common method in clinic of TCM.External therapy for internal disease is one of the characteristics of TCM treatment.Both traditional medicine and modern research had discovered that the aromatics produced an obvious effectiveness in external therapy.Percutaneous absorption is also a characteristic of Chinese medicine,the aromatic herb can promote the percutaneous absorption,and there are many methods of using aromatic herb in treating internal disease with external theropy.This article was a review on the theoretical basis and the clinical practice of aromatic herb.

Objective To compare the treatment effects and side events between chemoradiotherapy and radiotherapy alone for patients with primary cervical cancer.Methods Totally 197 patients with primary cervical cancer in our hospital from 2000 to 2006 were studied.They were divided into two groups: radiotherapy alone(RT)or cisplatin-based chemoradiotherapy(CRT).Group of RT included 100 patients who received external irradiation by ~(60)Co and intracavitary irradiation by ~(192)Ir.Group of CRT included 97 patients who received cisplatin-based chemoradiotherapy.The RT regimen was the same as group RT.Results The local control(CR+PR)in two groups had no statistic difference(P=0.500).The total 5 year survival rate in the two groups were 82% and 79%,with no significant difference(P=0.177);however,there was a significant difference for stage Ⅲ squamous cell carcinoma of the cervix,the 5 year survival rate being 56% and 84%,respectively(P