Objective To investigate the relationship of hypoxia inducible factor-1 α(HIF-1α),tumor necrosis factor-α (TNF-α),hepatocyte growth factor (HGF) and cerebral infarction.Method Two hundred and twelve cases with acute cerebral infarction in the Fourth People's Hospital of Langfang from Jun.to Dec.2013 were divided into progressive cerebral infarction(PCI) group(n =105) and the stability of cerebral infarction (SCI) group (n =107).Meanwhile 100 healthy people were served as control group.Enzyme-linked immunosorbent assay(ELISA) was applied to detect the levels of HIF-1 α,TNF-α and HGF.The PCI group were divided into small groups according to nerve function or infarction lesion size,and the levels of HIF-1 α,TNF-α and HGF of each group.Result The levels of HIF-1 α,TNF-α,HGF in PCI group were (2.3 ± 1.3) ng/L,(4.0 ± 0.5) mg/L and (1.4 ± 0.3) μg/L,significantly higher than those in SCI group ((1.1 ± 0.5) ng/L,(3.1 ±1.3) mg/L and (0.7 ±0.4) μg/L;F=5.42) and control group((0.5 ±0.1) ng/L,(1.8 ±0.4) mg/L and (0.4 ±0.1) μg/L;F =3.14).Meanwhile,the levels of HIF-1 α,TNF-α,HGF in SCI group was significantly higher than that of the control group (F =1.32,P ＜ 0.05).The levels of HIF-1α,TNF-α,HGF in mild PCI group were significantly lower than those in moderate group and severe group,and those in moderate group was lower than those in severe group (F =0.93,4.32,2.31 ; P ＜ 0.01).The levels of HIF-1 α,TNF-α,HGF in small infarction group were (0.6 ± 0.4) ng/L,(2.7 ± 0.4) mg/L,(0.7 ± 0.4) μg/L,significantly lower than those in infarction group ((1.1 ± 0.5) ng/L,(4.4 ± 0.5) mg/L,(1.1 ± 0.2) μg/L; F =4.71,P＜0.05) and large infarction group((1.4 ± 0.6) ng/L,(4.8 ± 0.6) mg/L,(1.9 ± 0.5) μg/L; F =2.09,P＜0.05).The levels of HIF-1α,TNF-α,HGF in focal infarction group was significantly lower than that in large infarction group and the difference is statistically significant(F =2.45,P ＜0.05).Conclusion HIF-1 α,TNF-α and HGF serum levels in progressive cerebral infarction are significantly increased,which is related to the function defect and size of infarction.

Humans ; Medical Staff ; Occupational Exposure ; prevention & control ; Occupational Health

Objective To explore the changes of IL-17 and neutrophils,eosinophils percentage in the induced phlegm from the children with different severity bronchial asthma.Methods Forty children with acute asthma were divided into two groups according to the severity of asthma:medium or severe group(n =16) and light group( n =24 ).Twenty normal children were chosen in the same stage as control group.The IL-17 content and the percentage of neutrophils and eosinophils were compared.Results The contents of IL-17 in the medium or severe group,light group and control group were(1.096 ±0.664) ng/L,(0.474 ±0.240) ng/L and(0.227 ±0.360 ) ng/L respectively.The percentage of neutrophils were ( 55.359 ± 12.486 ) ％,( 44.476 ± 17.708 ) ％ and ( 36.493 ± 12.470 ) ％ respectively.The percentage of eosinophils were ( 1.252 ± 2.025 ) ％,(4.107 ± 3.234) ％and (1.409 ± 3.480) ％ respectively.There were significant differences in three groups ( P ＜ 0.05 ).There was significant positive correlation between IL-17 content and percentage of neutrophils in the medium or severe group( r =0.740,P =0.049 ).There was negative correlation in the light group ( r =- 0.764,P =0.000 ).Conclusion There was different among IL-17 content and percentage of neutrophils,eosinophils in children of different groups.The study showed that IL-17 was involved in the potential pathogenesis of asthma.

Sinus histiocytosis with massive lymphadenopathy( SHML) is also called Rosai-Dorfman disease.It is a kind of benign lymphoid tissue proliferative diseases with unknown etiology.SHML appeared mostly in children and adolescent.It has diverse clinical manifestations accompanied with multiple organ inju-ry,and no clear laboratory indicators could support the disease,being a rare disease in pediatrics,easyot miss diagnosis.Thsi article reviewde the latest progress on diagnostis and treatment of SHML,to improve teh un-derstanding of the disease.

Objectives To explore the changes of serum interleukin-33 (IL-33), IgA anti-endothelial cell antibodies (AECA) and IgA anticardiolipin antibodies (ACA) in acute stage of Henoch-Sch?nlein purpura (HSP) in children and its clinical signiif-cance. Methods Thirty-seven children with acute HSP admitted to hospital were selected from Nov.2012 to Jan.2013. Twenty healthy children were selected as healthy controls. The serum levels of IL-33 were measured by double anti-body sandwich en-zyme-linked immunosorbent assay (ELISA) and the serum levels of AECA-IgA and ACA-IgA were detected by double antigen sandwich ELISA. Results Serum levels of IL-33, AECA-IgA and ACA-IgA in patients with acute HSP were signiifcantly higher than those in healthy controls (P<0.001). According to different clinical types, the serum levels of IL-33, AECA-IgA and ACA-IgA were signiifcantly higher in mixed-type HSP (with joint involvement and gastrointestinal symptoms and/or kidney damage) than those in HSP patients with only joint, abdomen or renal involvement (P<0.05). Serum levels of IL-33 in acute stage of HSP had a positive correlation with AECA-IgA (r=0.752, P<0.001) and ACA-IgA (r=0.788, P<0.001). Conclusions The immune pathological injury in vessels caused by the interaction of IL-33, AECA-IgA and ACA-IgA may be the important pathogenesis of HSP, which may provide a clue for the early diagnosis and treatment for HSP.

Objective To investigate the use of cardiopulmonary auscultation model in clinical skills teaching, and students' feeling about this teaching form. On the basis of this teaching model, we have reflected on the teaching mode. Methods Through the questionnaire survey, 145 medical stu-dents of grade 2009 were asked to investigate their learning experience about cardiopulmonary aus-cultation model and other aspects of it. Results There are 71.7% students liking cardiopulmonary auscultation model in teaching; 96.5% students can establish contacting abnormal cardiopulmonary auscultation signs with cardiovascular diseases after the training; There are 91.7% students thinking teaching hours about auscultation model is not enough. For the open question “what do you think is the difficulty of cardiopulmonary auscultation learning?” The representative answer is: I think car-diopulmonary auscultation is unable to recognize visual things. Conclusion Cardiopulmonary aus-cultation model can successfully improve the students' clinical skills and achieve good teaching effect. Students have a positive and supportive attitude to it.

ObjectiveTo explore the association between HLA -A gene and anaphylactoid purpura(AP) in children of Mongolia in Inner Mongolia. To find correlated genes and study part of pathogenesis and the method of prevention and cure of AP. MethodsThe method of case control was adopted and selected 56 children with AP as case group and 66 health children as control group in Mongolia,who had resided in Inner Mongolia three generations without consanguinity, history of mixed, marriages, other medical history , and family history of immunity,led into polymerase chain reaction sequence specific oligonucleotide probes technique, analyzed the type of HLA-A gene. The compare of gene frenquency made with logistic regression after χ2 or Fisher test. ResultsThe gene frenquency of HLA- A * 11 ( 16. 1% ) allele in case group compared to that of control group( 9. 1% ) ,Wald of HLA-A * 11 gene was 3. 954 ,P =0. 047, the difference had statistical significance. B = 0. 844 > 0, OR = 2. 325 > 1, it helped development of the disease,which 95％confident interval was 1. 012-5.340,which did not include 1 ,EF =0. 342 >0. ConclusionHLA-A * 11 allele may be the susceptible gene of AP in children of Mongolia in Inner Mongolia.

OBJECTIVE: To review the research on the anti-tumor effect of Dendrobium officinale at home and abroad in recent years. METHODS: Literatures about the anti-tumor effect of Dendrobium officinale in recent years were searched. The anti-tumor ingredients, pharmacological actions and clinical application of Dendrobium officinale were analyzed and summarize briefly. RESULTS: The polysaccharide and stilbenes in Dendrobium officinale showed anti-tumor effect. CONCLUSION: Dendrobium candidum may be applied to treat tumor clinically and be a candidate of the research and development of anti-tumor drugs.

Animals ; Bibenzyls ; chemical synthesis ; therapeutic use ; Imidazoles ; chemical synthesis ; therapeutic use ; Mice ; Pyrimidines ; chemical synthesis ; therapeutic use ; Sarcoma 180 ; drug therapy

BACKGROUND:An ideal scaffold material needs appropriate degradation rate and certain mechanical properties, but the traditional colagen sponge scaffold has rapid degradation velocity and low mechanical strength, which is easy to colapse and difficult to maintain its natural form. Traditional cross-linking methods also have the problems of cytotoxicity or colagen denaturation, severely limiting the application of colagen. OBJECTIVE:To design a new cross-linking method for colagen and to optimize the self-assembly process so as to develop a colagen sponge scaffold with good mechanical properties and resistance to degradation. METHODS:Colagens were modified by self-assembly technology to prepare colagen fibrils which were then freeze-dried into fibrilar colagen sponges. Meanwhile, we optimized the conditions of self-assembly by using orthogonal experiment based on univariate analysis of the effect of initial colagen mass concentration, final phosphate concentration and pH value on the conversion yield of colagen self-assembly. RESULTS AND CONCLUSION:We optimized the conditions of self-assembly revealed that the optimum conditions to prepare colagen fibrils were determined as pH=8.0, initial colagen concentration=2 mg/mL, and final concentration of phosphate=15 mmol/L. The results of scanning electron microscope showed that fibrilar colagen sponges were characterized by refined porous structure which was connected by colagen fibrils. In addition, the fibrilar colagen sponges showed better equilibrium-sweling ratio, water retaining property and mechanical strength compared with unmodified colagen spondages (P < 0.05), to solve the problems in rapid degradation.