1.Investigation on mercury poisoning event in a glass instrument and meter plant.
Chinese Journal of Industrial Hygiene and Occupational Diseases 2004;22(3):236-236
Air Pollutants, Occupational
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adverse effects
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China
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epidemiology
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Female
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Humans
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Industry
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Male
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Mercury
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urine
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Mercury Poisoning
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epidemiology
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etiology
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Occupational Diseases
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epidemiology
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etiology
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Thermometers
2.Detection of captopril based on its enhanced resonance light scattering signals of fluorosurfactant-capped gold nanoparticles
Journal of Pharmaceutical Analysis 2010;22(2):71-76,82
In this study, based on its enhancement effect on resonance light scattering (RLS) of fluorosurfactant (FSN)-capped gold nanoparticles (GNPs), we reported a simple approach for the rapid sensing of captopril. Under optimum conditions, the lowest detectable concentration of captopril through this approach (S/N=3) was 0.01μg/mL. The calibration curve was linear over the range of 0.08-4.0μg/mL for the detection of captopril. The recoveries of captopril were found to fall in the range between 99% and 100%. We have validated the applicability of our method through the analyses of captopril in pharmaceutical formulations. Good agreements were obtained for the determination of captopril between the present approach and official method.
4.Perioperative Nursing of Sacral Neuromodulation for the Treatment of Neurogenic Bladder
Chinese Journal of Rehabilitation Theory and Practice 2006;12(12):1048-1049
To summarize the experience about the preoperative and postoperative nursing of 11 cases with neurogenic bladder treated by sacral neuromodulation. The main nursing interventions of this procedure involved accurate recording of voiding diaries and postvoid residual, effective psychological nursing and health education of related disease information, standard videourodynamic examination. 11 patients acquired more or less benefit after the screening test and were discharged successfully, among them 2 cases had the permanent implantation of the stimulation device.
5.Drug susceptibility of wild-type and mutant H7N9 neuraminidase to zanamivir and oseltamivir.
Yan-Nan WEI ; Chao ZHANG ; Qing CHEN ; Ying GUO
Chinese Journal of Virology 2014;30(4):396-401
This study aimed to investigate the drug susceptibility of wild-type and mutant avian influenza A (H7N9) virus neuraminidase (NA) to oseltamivir and zanamivir. Codon optimized DNA of H7N9 (A/ Hangzhou/1/2013) NA was synthesized and constructed into the pcDNA3.1/His vector (NA(H7N9-WT)). Mutant NA(H7N9-H274Y) and NA(H7N9-R292K) plasmids were constructed by directed mutagenesis PCR using NA(H7N9-WT) plasmid as the template followed by sequencing. NA plasmids were transfected into 293T cells and cell lysates containing NAs were collected 48 h post-transfection. Wild-type and mutant NAs were analyzed by Western blotting and their activities were tested by the 4-MUNANA-based assay. All three NAs were expressed and enzymatic activities were confirmed. The effects of oseltamivir and zanamivir on all three NAs were then tested. It showed that the half maximal inhibitory concentrations (IC50s) of oseltamivir carboxylate on NA(H7N9-WT), NA(H7N9-H274Y) and NA(H7N9-R292K) were 1.6 nM, 15.1 nM, and > 1 000 nM with fold changes of 9 and > 625, respectively. The IC50 values of zanamivir on NA(H7N9-WT), NA(H7N9-H274Y), and NA(H7N9-R292K) were 1.1 nM, 1.4 nM, and 38.0 nM with fold changes of 1.3 and 34, respectively. These results indicated that oseltamivir and zanamivir could significantly inhibit NA(H7N9-WT). NA(H7N9-R292K) showed high-level resistance to both drugs (34-fold and 625-fold) and NA(H7N9-H274Y) was sensitive to both (1.3-fold and 9-fold). These results indicated that both oseltamivir and zanamivir could be used for patients infected with the H7N9 virus. However, when patients carried the H7N9 virus with a NA R292K mutation, other medications would be preferred over oseltamivir or zanamivir.
Antiviral Agents
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pharmacology
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Humans
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Influenza A Virus, H7N9 Subtype
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drug effects
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enzymology
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genetics
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Influenza, Human
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virology
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Microbial Sensitivity Tests
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Mutation
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Neuraminidase
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antagonists & inhibitors
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genetics
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metabolism
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Oseltamivir
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pharmacology
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Viral Proteins
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antagonists & inhibitors
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genetics
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metabolism
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Zanamivir
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pharmacology
6.Pediatric liver transplantation from split livers: a single-center experience
Shanni LI ; Nan MA ; Chao SUN ; Chong DONG ; Wei GAO
Chinese Journal of Organ Transplantation 2015;36(5):280-284
Objective To analyze and evaluate the efficay of split liver transplantation in children.Method From September 2006 to December 2014,210 children were treated with liver transplantation in Tianjin First Central Hospital.The clinical data were retrospectively analyzed and the difference in postoperative survival was compared between the groups.The 210 childrens were categorized into living donor liver transplantation group (183 cases) and split liver transplantation group (27 cases) based on their operation styles.In living group,all donors to recipients were immediate relatives within three generations.In split group,all donors were men,and livers were obtained from no heartbeat donors.Postoperatively,tacrolimus combined a duplex of prednisolone served as immunosuppression scheme.The survival and incidence of complications were observed.Result There was significant difference in the sex ratio between two groups (P<0.05).The donor liver cold ischemia time was significantly longer in split group than in living group (P<0.05).The 1-month,6-month,1-year and 2-year overall survival rate in 210 recipients was 99.5%,98.1%,96.2% and 94.2% respectively.The median follow-up time in living group and split group was 15.2months and 26.1 months,respectively.The 1-mont,6-month,1-year and 2-year survival rate was 99.5%,96.7%,92.6% and 74.1 % in living group,and 97.8%,96.2%,77.8% and 74.0% in split group,respectively (P<0.05).During the follow-up period,8 cases died (29.6%) in split group (5deaths due to infection and sepsis,and 3 deaths due to multiple organ failure),and 10 cases died (5.5%) in living group (6 deaths due to infection and sepsis,and 4 deaths due to multiple organ failure).Conclusion In the case of strict selection of donors,split liver transplantation can obtain good effect,but the incidence of complications is higher than living donor liver transplantation.Especially,the biliary complications should be prevented and managed actively.
8.Determination of eight heavy metal elements in iron dextran by inductively coupled plasma optical emission spectrometry and correction of spectral interference
Chao MA ; Simeng FANG ; Nan MI ; Kexin ZANG ; Mujun SU
Drug Evaluation Research 2017;40(3):335-340
Objective To establish a method for the determination of eight heavy metal elements (Pb,Cd,As,Hg,Co,V,Se,and Mo) in iron dextran by inductively coupled plasma optical emission spectrometry (ICP-OES).Methods Through detection wavelength selection,optimization of instrument parameters and applying interference element correction (IEC) method to correction of spectral interference,the eight heavy metal elements were analyzed by ICP-OES.Results The elements recoveries were from 88.7% to 101.3% by correction of spectral interference with IEC method.The accuracy of the method was good.The linearities of the detected elements were good,and the correlation coefficients were all greater than 0.999.The detection limits were from 0.12 to 7.26 ng/rnL.The quantization limits were from 0.40 to 23.96 ng/mL.The precision of the method was good (RSD<3.5%,n=6).Conclusion The results of the spectral interference correction by IEC method are greatly superior to the results of conventional method.The established method is accurate,sensitive,and rapid,which can be applied to the determination of contents of eight heavy metal elements in iron dextran.
9.Evaluation of dissolution consistency of Iloperidone Tablets
Nan MI ; Chao MA ; Simeng FANG ; Kexin ZANG ; Mujun SU
Drug Evaluation Research 2017;40(2):164-168
Objective To compare the dissolution curves of reference preparation and self-prepared Iloperidone Tablets in four different pH dissolution media (0.1 mol/L HC1 solution,pH 4.5 acetate buffer solution,pH 6.8 phosphate buffer solution,and water).Methods The solubility of Iloperidone in different pH solutions was measured,the dissolution curves of two preparations in four different pH dissolution media were determined by HPLC,and the similarity was investigated according to the f2 factor method.Results The f2 factors between reference preparation and self-prepared Iloperidone Tablets in four different media were more than 50.Conclusion The two preparations are equivalent in four different pH dissolution media in vitro.
10.Simultaneous Determination of 8 Heavy Metal Elements in Iron Dextran by ICP-OES
Chao MA ; Simeng FANG ; Nan MI ; Mujun SU ; Yunlei ZHANG
China Pharmacist 2017;20(3):579-583
Objective:To establish a method for the simultaneous determination of 8 heavy metal elements Pb, Cd, As, Hg, Co, V, Se and Mo in iron dextran by inductively coupled plasma optical emission spectrometry ( ICP-OES) . Methods:Through the detec-tion wavelength selection,the detection wavelength was confirmed as follows:220. 353 nm for Pb, 228. 802 nm for Cd, 188. 980 nm for As, 194. 164 nm for Hg, 228. 615 nm for Co, 311. 070 nm for V, 196. 026 nm for Se and 204. 598 nm for Mo. Through optimizing the instrument parameters, the optimal radio frequency power was 1. 3 kW, the nebulizer gas flow rate was 0. 7 L·min-1, and the pump speed was 10 r·min-1. By applying the above detection parameters, the 8 heavy metal elements were analyzed by ICP-OES simultaneously. Results: The linearity of the detected elements was good, and the correlation coefficients were all greater than 0. 9990. The detection limits were from 0. 12 to 7. 26 ng·ml-1 , the quantitation limits were from 0. 40 to 23. 96 ng·ml-1 and the recoveries were from 94. 1% to 103. 4% (RSD<3%, n=9). Conclusion: The method is specific, sensitive, rapid and accurate, which can be applied in the simultaneous determination of 8 heavy metal elements in iron dextran.