OBJECTIVE:To establish the quality criteria for Xiaoyan tablets. METHODS: Herba Taraxaci and Herba Violae in the formula were identified qualitatively by TLC and the content of Baicalin was determined by HPLC on Zorbax SB-C18(250 mm?4.6 mm,5 ?m) column with mobile phase consisted of acetonitrile-0.4%H3PO4(24∶76) at a flow rate of 1.0 mL?min-1. The detection wavelength was set at 280 nm and the column temperature was set at room temperature. RESULTS: Herba Taraxaci and Herba Violae could be detected by TLC. The linear range of Baicalin was 0.08～2.49 ?g(r=0.999 9). The average recovery rate was 100.24%(RSD=1.52%,n=6).CONCLUSION: The establish criteria are suitable for the quality control of Xiaoyan tablets.

China ; Hepatectomy ; methods ; trends ; Humans

Objective:To put forward some suggestions by comparing the current situation in both private and public hospitals. Methods:Comparing the basic information, scale, human resource and medical service management situation between the private and public hospitals by analyzing the 2014 Beijing health authorities report. Results:There are 355 private hospitals, 76. 1% of them are not rated and 59. 7% are not appointed for the medical insur-ance, and 82. 8% need to rent the land. Although the number of private hospitals is more than that of the public ones, their actual beds and health resources respectively account for only 20. 2% and 16. 4%. Besides, their in-come, assets and services also account for only about 10%. Conclusions:There is a big gap between the private and public hospitals in terms of scale, human resources and service ability. The Government should implement the policy about the ratings, medical insurance and the use of land which is given to support the private hospitals, and speed up the diversified medical model.

Objective: To establish human multidrug-resistant hepatocellular carcinoma cell line(QGY7703/DDP) to analyze its different resistance and sensitivity. Methods: The human hepatocellular carcinoma cell line QGY7703 was continuously grown in the presence of cisplatin（DDP) for up to 6 months. Cell proliferation and cytotoxicity were serially monitored. Results: The chemosensitivities to 5-FU，DDP，adramycin (ADM) and mitomycin(MMC) were different between QGY7703 and QGY7703/DDP. The exhibitions were 1.533 fold to 5－FU，2.181 fold to DDP，7.08 fold to ADM, 9.461 fold to MMC in QGY7703/DDP. Conclusion: The drug resistance in cancer cell lines can be established by exposing to antitumor drugs in cell culture system.

Objective To investigate the clinical curative effect of combined treatment of mometasone furoate nasal spray, loratadine, health education on children with allergic rhinitis and effects on IL-10 and IL-17. Methods 50 children with allergic rhinitis in our hospital from January 2015 to December 2016 were selected,and according to the different treatment methods divided into observation group and control group,25 cases in each groups.The control group were treated with loratadine, observation group with mometasone furoate nasal spray of loratadine + + health education treatment; we observed the two groups before and after treatment in children with nasal symptoms score, IL-10 and IL-17 and the incidence of adverse reactions, and the relevant data for comparative analysis. Results Mometasone furoate nasal spray of loratadine + + Health Education (observation group) the effect of treatment of children with allergic nasal is better than loratadine (control group) treatment, symptoms to improve the situation, IL-10 and IL-17 are better than the control group, the difference was statistically significant (P<0.05); two groups children with adverse reaction rate comparison, the difference was not statistically significant. Conclusion Children with allergic rhinitis selection of mometasone furoate nasal spray + loratadine + health education treatment effect significantly, can effectively improve the symptoms, IL-10 and IL-17, and will not increase the incidence of adverse reactions, it is worthy of clinical application.

0.97).The method of Real-time PCR and molecular beacon detection for bifodobacteria has many advantages,such as being sensitive,specific,simple and fast,and this method can be used in situ detection of bifidobacteria quantitatively.

Animals ; Carcinoma, Hepatocellular ; genetics ; virology ; Hepatitis B, Chronic ; complications ; Hepatitis C, Chronic ; complications ; Humans ; Liver Neoplasms ; genetics ; virology ; Neoplasm Recurrence, Local ; genetics ; Point Mutation ; Telomerase ; metabolism

Objective To study the effect of candesartan (Can) on the LPS‐induced proinflammatory cytokines release in VSMCs ,and to explore the role of TLR4‐mediated signaling pathway in this process .Methods Rat VSMCs were primarily cul‐tured ,and the effect of different concentrations of Can on VSMCs activity was observed by M TT assay .The cells were divided into 5 groups :A(control) ,B(LPS intervention) ,C(LPS + 10 - 7 mol/L Can) ,D(LPS + 10 - 7 mol/L Can ) and E (LPS + Can 10 - 5 mol/L Can) .mRNA and protein levels of Toll‐like receptor‐4(TLR4) ,myeloid differentiation primary response gene 88(Myd88)and nu‐clear factor‐κB(NF‐κB) nuclear translocation were determined by real‐time PCR and Western blot ,respectively ;IL‐1β and TNF‐αconcentration were detected by ELISA .The production of intercellular reactive oxygen species(iROS) was measured by the DCFH‐DA assay .Pretreating VSMCs with the inhibitors against TLR4 ,NADPH oxidase ,and NF‐κB ,or a combination with candesartan and these inhibitors ,and then the expression of IL‐1β and TNF‐α was measured by ELISA .Results Can in the concentration range of 10 - 8 - 10 - 3 mol/L had no significant effect onVSMCs activity .Compared with th control group ,Can could effectively inhibit LPS‐induced VSMCs ,IL‐1β and TNF‐α release ,decreased the mRNA and protein expression of TLR4 ,Myd88 ,reduced the produc‐tion of iROS ,inhibited the NF‐κB(p65) nuclear translocation with a concentration‐dependent manner(P< 0 .05) .Anti‐TLR4 anti‐body ,DPI ,and PDTC all inhibited LPS‐induced inflammatory cytokines release in VSMCs ,and the combination of Can and these blockers showed stronger anti‐inflammatory effect .Conclusion Can can decrease the releaseof inflammatory cytokines IL‐1β and TNF‐α in VSMCs stimulated by LPS ,which is realized by inhibiting the signaling pathway of TLR4/Myd88‐iROS‐NF‐κB .

OBJECTIVE:To observe therapeutic efficacy and safety of applying dexamethasone+docetaxel+cisplatin+fluoroura-cil(DCF)regimen induction chemotherapy before Ⅲ-Ⅳ stage oral squamous cell carcinoma resection. METHODS:A total of 120 patients with Ⅲ-Ⅳ stage oral squamous cell carcinoma were randomly divided into control group(60 cases)and observation group (60 cases). Both groups were given oral cancer resection,control group received radiotherapy 4-6 weeks after surgery,observation group was additionally given Dexamethasone acetate tablets 0.75 mg orally once a day,d1-7+Docetaxel injection 80 mg/m2 added in-to 0.9% Sodium chloride solution 250 mL intravenously for 2 h,d2+Cisplatin injection 80 mg/m2 added into 0.9% Sodium chloride solution 250 mL intravenously for 1 h,d2+Fluorouracil injection 750 mg/m2 added into 0.9% Sodium chloride solution 500 mL in-travenously,d2-5. Treatment course of 2 groups lasted for 7 d. Clinical efficacies of 2 groups were observed as well as EORTC QLQ-C30 score before and after treatment,recurrence rate 12,24,36 months and the occurrence of ADR. RESULTS:The short-term total response rate of observation group was significantly higher than that of control group;12,24,36 months fol-low-up recurrence rate of observation group were significant lower than those of control group;the incidence ofⅠ-Ⅱ degree nausea and vomiting,leucopenia in observation group were significantly higher than control group,with statistical significance (P<0.05);there was no statistical significance in the incidence of Ⅲ-Ⅳ degree nausea and vomiting,leucopenia andⅠ-Ⅱ,Ⅲ-Ⅳ de-gree diarrhea between 2 groups (P>0.05). Before treatment,there was no statistical significance in EORTC QLQ-C30 score be-tween 2 groups(P>0.05). After treatment,EORTC QLQ-C30 score of 2 groups were significantly lower than before,with statisti-cal significance (P<0.05),but there was no statistical significance between 2 groups (P>0.05). CONCLUSIONS:The applying of DCF regimen induction chemotherapy can improve short-term efficacy before Ⅲ-Ⅳ stage oral squamous cell carcinoma resec-tion,reduce reoccurrence risk but induce high incidence ofⅠ-Ⅱ degree nausea and vomiting,leucopenia.

AIM: To evaluate the effect of mizolastine in patients with ch ronic urticaria and to explore the role of IL-4 in the process of chronic urtic aria. METHODS: 32 cases of chronic urticaria were treated with m izolastine, and the therapeutic effects and the side-effect were evaluated. The serum IL-4 levels were tested by ELISA. RESULTS: The total ef fective rates were 62.5 % and 84.4 % (P 0.05 ), and before treatment were significantly higher than tho se after treatment (P