Objective To investigate the risk factors and microbial causes related to the pathogenesis of ventilator-associated pneumonia(VAP).Methods Totaly 138 intubated newborn infants for longer than 48 hours in Ningbo Women and Children's Hospital were studied,who were divided into VAP group (56 cases)and non VAP group(82 cases).Microbial causes of VAP were analyzed.Results Malnutrition,repeated tracheal intubation,repeated endotracheal suctioning,indwelling tube,posture,hyaline membrane disease,gestational age,birth weight,duration of intubation,duration of hospitalization were independent risk factors for ventilator-associated pneumonia(P<0.05).Klebsiella pneumoniae,pseudomonas aeruginosa,acinetobacter species and enterobacter cloacae were the main pathogenic bacteria of VAP.Conclusion Shortening of mechanical ventilation duration,avoiding repeated tracheal intubation and indwelling tube usage,semi-fowler positioning can lower the incidence of VAP.Pathogenic bacteria control is essential for VAP.

Due to the increase in competitive sports and some other reasons, the influence of physical activity in some extent, appeal to the essence of life care from its ethical. Excessive instrumental performance for sports ac-tivities, utilitarianism, in some ways is not pure is the activity of fitness and it happened some alienation. There-fore, the relevant state departments and the whole society starting from the height of national strategy, deal with the relationship of sport and fitness sport, promote the development of undertakings of physical culture and sports, mak-ing the sports return to nature of life care.

Objective:To study the expression of discoidin domain receptor1(DDR1) protein in esophageal squamous cell carcinoma(ESCC) tissues. Methods:The expression of DDR1 protein was detected by Western blotting in 40 cases of ESCC tissues,para-cancer tissues,and matched esophageal normal tissues,and their relation to clinical pathologic characteristics of esophageal cancer was analyzed. Results:The expressionof DDR1 in ESCC(1.201?0.348)were significantly higher than those in para-cancer tissues(0.640?0.323)and normal esophageal tissues(0.551?0.413). The positive expression rate and relative content of DDR1 in ESCC were correlated with degrees of differentiation、lymphnode metastasis and invasion depth of carcinoma (P

OBJECTIVE:To improve HPLC for the contents determination of 2-methyl-5-methoxy-indole-3-acetic acid(impuri-ty Ⅰ) and 4-chlorobenzoic (impurity Ⅱ) in Indometacin enteric-coated tablet. METHODS:The column was Phenomenex Phe-nyl-Hexyl with mobile phase of 10 g/L acetic acid solution- acetonitrile(gradient elution)at a flow rate of 1.0 ml/min,the detec-tion wavelength was 254 nm,column temperature was 40℃,injection volume was 20 μl. RESULTS:The linear range was 0.262 2-5.243 0 μg/ml for impurity Ⅰ(r=0.999 8)and 0.252 5-5.050 0 μg/ml for impurity Ⅱ(r=0.999 8);the limits of quanti-tation were 1.12 ng and 0.48 ng,limits of detection were 0.340 ng and 0.146 ng,respectively;RSDs of precision,stability and re-producibility tests were lower than 2%;recoveries were 99.71%-100.52%(RSD=0.28%,n=9) and 100.84%-102.14%(RSD=0.47%,n=9). CONCLUSIONS:The method is accurate and rapid,and can be used for the contents determination of impurity Ⅰand impurityⅡin Indometacin enteric-coated tablet.

OBJECTIVE: To establish the HPLC method for the determination of spironolactone tablets. METHODS: The determination was performed on Agilent C18 column with mobile phase of acetonitrile-water (50 ∶ 50) and detection wavelength of 238 nm. RESULTS: The linear range of spironolactone were within 5.023 1 ?g?mL-1～75.347 1 ?g?mL-1 (r=1.000 0) with an average recovery of 100.23% (RSD=0.53%). CONCLUSION: The method is reproducible, simple and convenient for the quality control of spironolactone tablets.

Objective To analyze the clinical features of rheumatic diseases patients with Glucocorticoid (GC) induced osteoporotic fractures.Methods Fourteen patients with GC induced osteoporotic fractures in Beijing Children's Hospital from January 2005 to December 2012 were retrospectively analyzed.The clinical features,spine radiographs/CT,dose of GC were analyzed.Results In various rheumatic diseases,systemic lupus erythematosus (SLE) and systemic onset juvenile idiopathic arthritis (SoJIA) were the most common related diseases.All children had no obvious causes of fracture.Eleven patients had back pain or limitation of activity.Fourteen patients had vertebral fractures.Seven cases had thoracic vertebral fracture,3 cases had fractures of lumbar vertebra.Four cases had multiple painful vertebral fractures.The time of fracture occurrence in patients received glucocorticoid therapy were between 5 months and 6.5 years.Daily minimum dose of glucocorticoid was 0.25-1.12 mg/kg and mean dose was 0.47-2.62 mg/kg.Conclusion GC induced osteo-porotic fractures is a serious complication of long-term use of glucocorticoid.Physicians taking care of children who have received long-term glucocorticoid therapy should be alert of the manifestation of osteo-porosis,such as back pain.

BACKGROUND:Bone marrow mesenchymal stem cel s can migrate into tumor tissues, as reported in recent studies.
OBJECTIVE:To investigate the tropism and effect of transplantation of bone marrow mesenchymal stem cel s on proliferation and differentiation of gastric cancer cel line SGC7901 in rats.
METHODS:Gastric cancer models were established in Sprague-Dawley rats by subcutaneous injection of gastric cancer cel line SGC7901, and then, in cel transplantation group, each rat underwent an intraperitoneal injection of 1×107 bone marrow mesenchymal stem cel s. After transplantation, the targeting ability of bone marrow mesenchymal stem cel s was detected using fluorescent DiI labeling. Cyclin D2 mRNA and protein expressions were measured using real-time PCR and western blot assay, respectively. Apoptosis of gastric cancer cel s was observed by in situ terminal labeling method.
RESULTS AND METHODS:Bone marrow mesenchymal stem cel s successful y migrated into the gastric cancer site in rats. The expression levels of cyclin D2 mRNA and protein in the cel transplantation group were significantly higher than those in the model group (P<0.05). It was observed that the number of apoptotic cancer cel s was significantly reduced in the cel transplantation group compared with the model group (P<0.05). These findings indicate that bone marrow mesenchymal stem cel s have the ability to migrate into the tumor sites, thereby promoting the proliferation of gastric cancer cel s.

Klebsiella Pneumoniae is a opportunity infection and nosocomial infection bacteria in Enterobacteriaceae,which can lead to serious infection or death.Klebsiella Pneumoniae leads to increased infections proportion in recent years,which resisits to many common antibiotics.Clinical drug resistant strains include Carbapenem-resistant Klebsiella Pneumoniae,Multidrug-resistant Klebsiella Pneumoniae,Extensively drug resistant bacteria and Pandrug-resistant Klebsiella Pneumoniae.Researches beared out that the risk factors of acquiring nosocomial infection of Klebsiella Pneumoniae are state of an illness,time of hospitalization,mechanical ventilation,the kind and number of antibiotics.

The treatments for ankylosing spondylitis (AS) are still not effective, which partially due to its unclear patho?genesis. Interleukin (IL)-23/IL-17 axis is a significant immunologic pathway which has attracted much attention in the past few years. This article reviewed the role of IL-23/IL-17 axis in genetic and immunological pathogenesis of AS, and intro?duced the recent progresses in treatments of AS using anti-IL-23 or anti-IL-17 antibody. The important role of IL-23/IL-17 axis in the pathogenesis and treatment of AS indicates its potential use as a new therapeutic target.

Objective:To investigate the effect of Sec-O-Glucosylhamaudol on inflammatory mediator and cytokine( NO and IL-6)production in Lipopolysaccharide(LPS) stimulated murine macrophage(RAW264.7).Methods: Macrophages were induced with LPS,and incubated with different concentrations of Sec-O-Glucosylhamaudol(20,40,80 μg/ml),the quantity of NO production was measured by Griess reagent;the IL-6 production were measured by enzyme linked immunosorbent assay(ELISA),the expression of nitric oxide synthase( iNOS) in cells were detected by Western blot;the expression of iNOS and IL-6 mRNA were analyzed by real-time PCR.Results:Each concentrations of Sec-O-Glucosylhamaudol(20,40,80 μg/ml) inhibited the production of NO and IL-6 in LPS-stimulated RAW264.7 cells(P<0.01).This compound also reduced the mRNA expression of iNOS and IL-6.Conclusion:Sec-O-Glu-cosylhamaudol exhibited anti-inflammatory activity by inhibited the NO and IL-6 production in LPS stimulated RAW264.7 cells.