Objective To observe the expression of macrophage migration inhibitory factor (MIF) in colon tissues of rats with 2,4, 6-trinitrobenzene sulfonic acid (TNBS) induced colitis and the relationship between MIF and the severity of the colitis. Methods 24 Spra-gue-Dawley rats were randomly divided into three groups: normal group, model group Ⅰ and model group Ⅱ. Rat colitis model was induced by 2,4,6-trinitrobenzene sulfonic acid (TNBS), and disease activity index (DAI) was calculated every day. The myeloperoxidase (MPO) ac-tivity was detected by biochemistry, the expression of MIF in colon tissue was detected by semi-quantitative reverse transcriptional chain reac-tive (RT-PCR). Results In normal group, DAI, MPO activity and MIF mRNA level were 0.51±0. 28,0. 38±0. 18,0. 11±0. 03, while in model group Ⅰ were 5.04±0.73,1.68±0. 45,0. 65±0.04 and in model group Ⅱ were 8. 13±0.71,2. 70±0. 35,0. 81±0. 05. Com-pared with normal control, the expression of MIF mRNA significantly increased (P<0.05), MPO activity and DAI also significantly in-creased(P<0.05)in model group, especially in model group Ⅱ. Conclusion The expression of MIF increased in experimental colitis, and there was a positive relation between MIF expression and the severity of colitis, which suggested that MIF may be related to the pathogen-esis of TNBS-induced colitis.

Objective To study the relationship between operative procedures and complication of colorectal carcinoma with acute colonic obstruction in the elderly patients. Methods 65 elderly patients undergoing emergency operation for acute colonic obstruction were analyzed retrospectively. One stage tumor resection and anastomosis was performed in 25 cases with right-side colonic lesion and in 26 cases with left-side colonic lesion, Hartmann operation in 8 cases, Dixon operation in 4 cases and proximal colostomy in 2 cases. Results In 65 cases, 13 cases of postopera-tive complications,the rate was 20.0% (13/65) ,which infected with toxic shock two cases,four cases of pulmonary infection,anastomotic leakage two cases, wound infection, split 2 cases, death three cases, the mortality rate was 4.6%. The rates of postoperative complications in right-side colonic lesion, left-side colonic lesion, Hartmann opera-tion and Dixon operation were 69.2% ,0,30.7% and 0 respectively(P < 0.05 ～ 0.01). Conclusion Acute obstruc-tion of colon cancer,the right-side colonic lesion were safe,feasible and few complications in colorectal carcinoma with acute colonic obstruction.

In the tumor microenvironment, inflammatory cells regulate intracellular signaling transduction mediated by the corresponding nuclear transcription factor by releasing inflammatory factors ,which leads to transformation of epithelial cells to mesenchymalcellsand eventually promote tumor metastasis. Because of the intimate relationship between inflammation and tumor metastasis , it would be helpful for further enhancement of effectiveness of tumor therapy if we could better understand both the biological character of tumor and the related inflammatory factors.

Aim To observe the effects of heat shock protein 70 ( Hsp70 ) activator SW02 on lipopolysaccha-ride( LPS)-induced expression of inducible nitric oxide synthase ( iNOS ) and LPS-induced production of nitric oxide ( NO ) in macrophages. Methods RAW264. 7 cells were stimulated by LPS, and were divided into DMSO,DMSO+LPS(1 mg·L-1),SW02,and SW02+LPS ( 1 mg · L-1 ) groups. The protein expression was detected by Western blot. NO concentration was measured by Griess kit. The iNOS mRNA was detected by real-time PCR. The NF-κB binding to iNOS promot-ers was measured by chromatin immunoprecipitation ( ChIP ) assays. Results SW02 significantly blocked the protein and mRNA expression of iNOS as well as the production of NO in LPS-stimulated RAW264 . 7 cells(P<0. 01 or P<0. 05,SW02+LPS group vs DM-SO+LPS group) . SW02 did not affect the LPS-induced degradation of IκB-α( P>0. 05 , SW02 +LPS group vs DMSO+LPS group ) and nuclear translocation of NF-κB ( P > 0. 05 , SW02 + LPS group vs DMSO + LPS group) . However,SW02 reduced the NF-κB binding to iNOS promoters inside the cell( P<0. 05,SW02+LPS group vs DMSO+LPS group) . Conclusion These re-sults show that SW02 prevents iNOS expression and NO induction likely through attenuation of the NF-κB bind-ing to iNOS promoters in macrophages.

Objective To investigate the clinical effect of compound Chinese medicine fumigation granules on surgical treatment of anorectal disease patients and the impact of postoperative complications and wound healing.Methods 78 cases of patients anorectal surgery from June 2013 to February 2016 in our hospital anorectal were admitted,and were randomly divided into observation group and control group,39 cases in each group,using anti-inflammatory and conventional dressing treatment after operation in both two groups,the observation group was treated with compound Chinese medicine fumigation granules,treatment for 10 days continuous.The changes of wound healing and related clinical symptoms were observed and compared between the two groups.Results Third,7,14 days after operation,VAS scores in observation group were lower than the control group,the differences were statistically significant(P<0.05).Tirst days after operation,there was no significant difference in perianal edema,anal bulge,anal dampness and bleeding symptom score between the observation group and the control group; third,7 days after surgery,the perianal edema,anal bulge,anal wet,bleeding symptom score in observation group were lower than the control group,the differences were statistically significant(P<0.05); fifth,14 days after operation,wound patients smoked wash reduction rate in observation group was higher than the control group,the differences were statistically significant(P<0.05); observation group average wound healing time was(16.3±2.8)days,the control group was(18.4±3.3)days,the differences were statistically significant(P<0.05).Conclusion After surgical treatment of anorectal disease,use the compound Chinese medicine fumigation particles for treatment can help relieve the clinical symptoms of patients,reduce pain,promote wound healing.

To study the fingerprints of liposoluble components in Salvia yunnanensis roots collected from different habi-tats, and compare with the fingerprints of Salvia miltiorrhiza. Methods: The fingerprints were determined by HPLC and the results were analyzed by a similarity evaluation system for chromatographic fingerprints of TCM ( Version 2004 A) . Results:The fingerprints of different samples of Salvia yunnanensis roots showed high similarity. And the characteristic peaks in the fingerprints of the liposoluble components were basically the same as those of Salvia miltiorrhiza, while the contents of several main effective components were lower than those in Salvia miltiorrhiza. Conclusion: The liposoluble components in Salvia yunnanensis roots show relatively high similarity with those in Salvia miltiorrhiza, however, there are obvious differences in the contents of the main constituents.

The infrapatellar fat pad(IFP),also known as the Hoffa's fat pad,is an intracapsular, extrasynovial structure that fills the anterior knee compartment,and is richly vascularized and innervated. It involved knee joint biomechanics,damaged cells repairing and some other processes.Factors such as trauma or operation lead to pathological changes,including fibrosis,edema,and inflammation,and finally result in disorder syndrome.IFP was discovered and named by German surgeon Albert Hoffa in 1904.It is one of the main causes of knee pain under the patellar.Clinically,it is mainly diagnosed by physical exam-ination and MRI.Treatments include physical therapy,local anesthetics,arthroscopic resection,etc.

Type Ⅱ innate lymphoid cells is an emerging family of innate lymphocytes mainly distributed in mucosal tissues such as the lungs,intestines,skin,etc.These cells are capable of producing Th2-type cytokines including IL-5 and IL-13 in response to IL-25 and IL-33.ILC2s has attracted much attention for its important roles in anti-infection immunity and allergic diseases in respiratory tract.Studies have shown that ILC2s represented a crucial source of Th2-type cytokines and could regulate the adaptive immune response in allergic airway diseases.Meanwhile,ILC2s was able to fulfill vital functions in parasite clearance, anti-virus infection and subsequent tissue repair.Therefore,research in ILC2s phenotype,development and function has great practical significance.This paper reviews the definition, classification, developmental regulation of ILC2s with a particular focus on its role in respiratory mucosal homeostasis and lung-related diseases.

Objective To compare two types of digestive reconstruction and postoperative complications after total gastrectomy in patients with gastric carcinoma. Methods Roux-en-Y esophagojejunostomy and P-type Roux-en-Y esophagojejunostomy were performed in 50 patients with gastric carcinoma after total gastrectomy. The operating time and postoperative complications were observed. Results The gastrointestinal symptoms for Roux-en-Y esophagojeju-nostomy and P-type Roux-en-Y esophagojejunostomy serve as : diarrhea ( 11.8% and 12.1% ), dumping syndrome (3.9% and 3.3% ) and reflux esophagitis(5.9% and 6.0% ) ,the incidence of the difference was not significant ( than,P>0.05 ). The postoperative complications in P-type Roux-en-Y esophagojejunostomy( 13.1% ) slightly higher that Roux-en-Y esophagojejunostomy( 11.1% ) ,but the difference was not significant( P>0.05 ). Roux-en-Y esoph-agojejunostomy average operative time(3.1±0.3)h was significantly reduced than P-type Roux-en-Y esophagojeju-nostomy(3.7±0.2)h(t=3.35,P<0.05). Conclusion Roux-en-Y esephagojejunostomy and P-type Roux-en-Y esophagojejunostomy is a suitable type after total gastrectomy for patients with gastric carcinoma, but the former opera-tive time was shorter.

Evaluation of regional health service system can provide guidance for regional health planning,improve the ability of the national macro-control and supervision,and optimize the medical resource allocation.China has yet to establish a set of scientific and comprehensive evaluation index system.Through the introduction of the importance,influence factors and the current methods in evaluation of regional medical service system in US,suggestions are provided for establishing and improving China's evaluation system of regional health service.