Objective To analyze the perceived stress level and health risk stress (HRS) status of pediatric medical staff, and its influencing factors. Methods A total of 685 pediatric medical staff from Guangdong Women and Children Hospital were selected as the study subjects using the convenience sampling method. The Chinese Perceived Stress Scales (CPSS) was used to evaluate their perceived stress level, and the HRS detection rate was calculated. Results The average CPSS score was (33.2±8.4) points. The detection rate of HRS was 68.6% (470/685). The result of multivariate logistic regression analysis showed that age, education level, job position and professional title were independent influencing factors for HRS in pediatric medical staff (all P<0.01). The risk of HRS was higher in the staff in age group of 35-< 45 years than that in the age group of < 35 years [odds ratio (OR), 95% confidence interval (95%CI) was 2.449 (1.558-3.849), P<0.01]. The risk of HRS in the staff in education groups of college, bachelor and master's degree was lower than those in doctoral degree group [OR and 95%CI were 0.171 (0.069-0.427), 0.206 (0.090-0.471) and 0.249 (0.118-0.524), respectively, all P<0.01]. Pediatricians had a higher risk of HRS than pediatric nurses [OR and 95%CI was 5.545 (3.268-9.409), P<0.01]. Medical staff with primary and intermediate professional titles had a higher risk of HRS than those with associate/senior titles [OR and 95%CI were 4.382 (1.793-10.711) and 4.586 (1.886-11.151), respectively, both P<0.01]. Conclusion The perceived stress of pediatric medical staff was at a relatively high level, and the situation regarding HRS is concerning. Age, educational level, job position, and professional title are risk factors of HRS.