Objective To investigate the effects of allergen at different concentrations on the production of IL 5 by lymphocytes of mice with asthma and its mechanism. Methods The levels of IL 5 produced by T cells treated with allergen of different concentrations and monocytes as antigen presenting cells in vitro were measured by ELISA and the apoptosis rate of T lymphocytes was determined by TUNEL. Results ① The administration of high dose of allergen inhibited the expression of IL 5( P

Objective To observe the effects of mouse indoleamine 2,3-dioxygenase (IDO) gene on CD4+ T cells by transfecting an eukaryotic expression vector containing mouse IDO gene fused with enhanced green fluorescent protein (pEGFP-N1). Methods The immature dendritic cells (imDCs) derived from mouse bone marrow were cultured in vitro and morphologically identified by transmission electron microscope, scanning electron microscope and flow cytometry. The immature dendritic cells were transfected by DOTAP liposome, and the expression of green fluorescence protein was confirmed with inverted fluorescence microscope. The spleen-derived CD4+ T lymphocytes from mice were isolated and purified in vitro, and then carried to mixed lymphocyte reaction (MLR) with 3H-TdR incorporation. The effect of IDO gene on the proliferation of spleen-derived CD4+ T lymphocytes of the allergized mouse challenged by ovalbumin was assayed by 3H-TdR MLR incorporation, and the apoptosis of spleen-derived CD4+ T lymphocytes was assayed by TUNEL. Results The proliferation of spleen-derived CD4+ T lymphocytes of the allergized mouse challenged by ovalbumin were inhibited by the imDCs transfected with IDO gene, the number of CD4+ T lymphocytes treated with the imDCs transfected pEGFP-N1-IDO eukaryotic expression vector decreased significantly compared with that in control group (P

The procedure to delineate the maximal forced inspiratory flow volume curve(MIFVC)was described and the curve was determined in 56 normal subjects to serve as the control and 62 patients with various chronic obstructive pulmonary diseases(COPD).It was found that MIFVC was significantly lower in patients with various COPD than in the control(P

Objective To explore the bronchial obstruction reversibility in patients with chronic obstructive pulmonary disease, and to conduct a comparison with that in patients with asthma. Methods The pulmonary ventilation function of patients with chronic obstructive pulmonary disease(COPD) and with asthma before and after inhaling salbutamol inhaler was separately determined with 6200 Autobox DL, and all corresponding parameters of in two groups were compared. Results No difference of the corresponding parameters of baseline ventilation function in two groups was found. The pulmonary function of patients in two groups was obviously improved after inhalation of drug( P

0 05). Excessive bronchoconstriction occurred in one patient (1/41, 2.44%). Nausea and vomiting, pharyngeal dryness and pharyngalgia, dizziness and headache, discomfort of chest, and so on were found in other 43 9% patients, but the side effects were mild and endurable. Conclusion Sputum induction after inhalation of short acting ? 2 agonist is safe for asthmatic patients in the remission stage.

Objective:To investigate the relation between expression of Bcl-2,Bax and eosinophils(Eos) apoptosis,explore effect of Bcl-2 and Bax on asthma.Methods:Guinea pigs were divided into normal and asthmatic group randomly. Ovalbumin was used to prepare model of asthma in guinea pig. Apoptosis of Eos in BALF was detected.mRNA expression of Bcl-2 and Bax in Eos were measured by hybridization and RT-PCR.Results:Comparing with normal group,Eos in BALF from asthmatic group were significantly higher; the percentage of apoptotic Eos from asthmatic group were significantly lower( P

BACKGROUND: Wnt signaling pathway is a key regulator of cellular proliferation and differentiation, but its correlation with neural differentiation of bone marrow mesenchymal stem cells (BMSCs) is not very clear. OBJECTIVE: To find out the molecules of the Wnt family which are involved in differentiation of rat BMSCs into neuron-like cells. METHODS: The rat BMSCs were separated and cultured in vitro. The morphology of the BMSCs was observed. Flow cytometry analysis was performed to detect cell phenotype CD44, CD9, CD34 and CD45. Wnt3a and Wnt5a were respectively combined with basic fibroblast growth factor to induce BMSCs differentiation into neuron-like cells, and then were identified by using immunocytochemistry and RT-PCR. RESULTS AND CONCLUSION: The BMSCs were long-spindle. CD9 and CD44 were highly expressed, while CD34 and CD45 were lowly expressed. Nestin and neuron specific enolase were positive but glial fibrillary acidic protein were not obviously expressed when they were cultured with Wnt3a. In Wnt5a group, Nestin expression was weakly positive, while neuron specific enolase and glial fibrillary acidic protein were negative. RT-PCR result revealed Nestin expressed both before and after induction in the Wnt3a induced group, neuron-specific enolase exhibited apparent amplified bands 5 days after the induction, and more apparent at 10 days. A weak amplification band of glial fibrillary acidic protein could be seen at 10 days after the induction. In Wnt5a and control groups, BMSCs induced by 10 days weakly expressed Nestin, while neuron-specific enolase and glial fibrillary acidic protein were almost not expressed. It is indicated that Wnt3a molecule can promote the differentiation of BMSCs cultured in vitro to neuron-like cells.

Objective　To investigate the relationship of the expressions of common β (βcR) and specific α chains of IL-5, IL-3 and GM-CSF receptors with the eosinophils（Eos） apoptosis for the roles of these receptors in asthma. Methods　All of 12 guinea pigs were equally randomized into normal and ovalbumin sensitized asthmatic group. The apoptosis of hypodense and normodense Eos in bronchoalveolar lavage fluid (BALF) was detected by TUNEL method. The expression of common β and specific α chains of the 3 receptor mRNA were measured with RT-PCR and in situ hybridization. Results　More Eos were observed in asthmatic group than in normal group, especially those in hypodense (P<0.01). The apoptotic rate of Eos in BALF were significantly lower in asthmatic group than in normal animals (P<0.01), but no difference was found between hypodense and normodense Eos. Compared with normal group, the expressions of the 3 receptor mRNA α chains were decreased in asthmatic group than in normal group (P<0.01), but βcR expression was increased significantly. More IL-3 Rα and GM-CSFRα mRNA were expressed in hypodense Eos than in normodense Eos. The contents of IL-5Rα and IL-3Rα mRNA were lower in asthmatic group than in normal group. Conclusion　The apoptosis of Eos is inhibited in BALF from guinea pigs with asthma. The expressions of IL-5, IL-3 and GM-CSF receptors in Eos are regulated through 2 mechanisms: the reduced expressions of their specific α chains which attenuating the negative regulation on Eos activation and inhibited apoptosis and the increased expressions of their βcR which enhancing the positive regulation on the 2 aspects. These data suggest that IL-5, IL-3 and GM-CSF receptors might be involved in the pathogenesis of asthma through regulating the apoptosis of Eos.

Objective To compare the efficacies of laparoscopic colorectal resection and conventional open surgery by collecting and investigating related studies with Meta-analysis.Methods Randomized controlled trials(RCT) on the outcomes of laparoscopic colorectal resection and conventional open surgery that published between January 1989 and March 2008 in English were collected and analyzed.The quality of the original investigations was evaluated,and the low-level reports were excluded.Then the odds ratio(OR) for the incidence of early postoperative small bowel obstruction between the two methods were calculated.ResultsTotally 12 studies including 3032 cases were analyzed.Among which,1522 patients received laparoscopy,33 of them developed early postoperative small bowel obstruction.1510 cases underwent open surgery,and 71 of these patients showed small bower obstruction before being discharged form hospital.The pooled OR was 0.46 with a 95% confidence interval of(0.30-0.69).Conclusion Compared with open surgery,laparoscopic colorectal resection is superior in the incidence of early postoperative small bowel obstruction.

The fatigue of the diaphragm was determined with electromyography ir.35 patients with chronic obstructive emphysema (COPD) and in 47 nomaJ subjects serving as the control.It was found that the high/low frequency ratio (H/L) and the central frequency (Fc) of the diaphragmatic electromyogiam (EMGdi) were markedly decreased after diaphragmatic fatigue in COPD patients as well as in the controls ( P