Objective To investigate the effects of allergen at different concentrations on the production of IL 5 by lymphocytes of mice with asthma and its mechanism. Methods The levels of IL 5 produced by T cells treated with allergen of different concentrations and monocytes as antigen presenting cells in vitro were measured by ELISA and the apoptosis rate of T lymphocytes was determined by TUNEL. Results ① The administration of high dose of allergen inhibited the expression of IL 5( P

A medical information service platform for international peacekeeping was designed according to the in-formation needs of medical team, laws, publications and websites in relation with international peacekeeping, which can meet the information needs of peacekeeping officers and soldiers. Literature and information resources will be richened and perfected on the platform in the coming days in order to meet the different and personal infor-mation needs of peacekeeping officers and soldiers.

The great changes taking place in the environment of academic library were analyzed from the aspects of networked information environment, knowledge-based user needs and homogenized library resources. The main up-date development characteristics of academic library were described according to the current development situation in foreign and domestic academic libraries as revealed in our investigation, such as e-only of library resources, knowledge-based and embedded information service, associated, semantic and integrated information organization, incorporation of primary information resources into institutional knowledge property management, digital library emerged in scientific data monitoring centers, SoLoMo as the concept for creating the next generation mobile library service. It was pointed out that academic library will become the user-oriented knowledge service center that provides user-driving knowledge service and intelligent knowledge service in an all-inclusive knowledge management manner. It is thus necessary for academic library to attach more importance on the information needs of scientific workers, to strengthen the user-oriented professional work layout, application of information technology, interlibrary coopera-tion and resources-sharing, and to improve the information literacy of librarians.

The problems in medical information resources existed at present were analyzed according to the increased medical service in military operations of Chinese PLA during the non-war period, such as scattered resources and poorly targeted information, with suggestions and methods put forward for integrating the related resources in order to improve the medical support ability in military operations during the non-war period .

ction of immune tolerance of T cells to allergens.

Objective To improve the methods for in vitro generating dendritic cells (DCs) from mouse bone marrow and to identify it with morphological, phenotype determination. Methods Cells isolated from mice bone marrow were cultured in GM-CSF (20 ng/ml), differentiating into dendritic cells. Morphological changes were observed by optical phase contrast microscopy, and surface molecules including CD11 C, CD80, MHCⅡ were detected by FACS. Results A large number of typical DCs were observed after culturing for 10 d. FACS analysis showed that the amplified DCs could express CD11 C, CD80, MHCⅡ. Conclusion A large quantity of highly pure BM-DC can be obtained by this method.

Objective A morphometric study was carried out on GABAergic neurons and astrocytes in primary auditory cortex of 4 young cats (1-3 years old, 20-2.5 kg) and 4 old cats (10-13-year-old, 3-3.5 kg), in order to explore the age-related changes and the probably physical effects underlying these changes. Methods The same regions of the primary auditory cortex (AI) were comparatively studied. Nissl staining and immunohistochemical methods were applied to explore the differences of GABAergic neurons and astrocytes(specifically labeled by GFAP as a mark as antibody) in the primary auditory cortex(Al) between young and old cats. Serial cross-sections from two age groups were stained with Nissl for layer structure observation and demonstration of neurons; Adjacent sections were stained with anti_GABA immunohistochemistry for localizing and characterizing GABAergic and with anti-GFAP for astrocytes in AI. Results The diameter, density and number of GABAergic neurons in Al of old cats declined significantly compared with the counterpart in young cats, especially in layers Ⅱ,Ⅲ of AI; but the change in GFAP-IR cells was reversed: old cats showed clearly larger number and stronger staining of GFAP-IR cells than that in young cats. The GFAP-IR cell bodies and dendrites swelled obviously in old cats, especially in layer Ⅰ of AI and in white matter. Conclusion The result presented a direct morphological evidence for weakness of GABAergic intracortical inhibition, which is considered to be a possible mechanism for age-related acoustic function decline. The enhanced function of astrocytes may play an important role in decreasing the GABA content in AI of the old individuals.

〔Abstract〕 By use of the decision tree algorithm and diagnostic indexes , the paper sets up the discrimination rules to make differential diagnosis of Primary Hepatocelluar Carcinoma ( PHC) based on basic data of 95 patients with PHC and 190 patients with liver cirrhosis , in-cluding the CT diagnosis, testing results of imaging and serologic markers such as the HbsAg , AFP, CEA and AFU, sex and age, etc.As indicated by the results , the data mining technology represented by the decision tree can support the differential diagnosis of PHC .

OBJECTTo study the significance of the ultra-high power microscope in the examination of vaginal discharge.

METHODSBy the ACT-2000 ultra-high power microscope system and Olympus CX21 microscope, the vaginal discharge of 1,100 gynaecology out-patients was examined respectively.

RESULTSThe positive rate of mould in the patients was 11.55% by CX21 and was 20.27% by ACT-2000, respectively. The positive rate of trichomonas vaginalis was 2.55% by CX21 and 3.0% by ACT-2000, respectively. The clue cell was detected in 11.27% of the patients by ACT-2000, but no such cell reported by CX21. Totally, positive results were obtained in 14.09% of the patients by CX21 and 32.55% by ACT-2000.

CONCLUSIONBy using the ultra-high power microscope, the positive result can be increased obviously in the examination of vaginal discharge. It is very important in clinical practices.

Adult ; Female ; Humans ; Microscopy ; methods ; Microscopy, Electron ; methods ; Middle Aged ; Vaginal Discharge ; pathology ; Young Adult

Objective: To observe the expression of hMLH1 protein in esophagus squamous cell carcinoma, atypical hyperplasia tissue and normal esophagus tissue, so as to discuss the relationship between hMLH1 expression and esophagus carcinogenesis. Methods: The specimens of esophagus squamous cell carcinoma, atypical hyperplasia and normal esophagus tissue were obtained from 92 esophagus carcinoma patients. Immunohistochemistry (IHC) staining technique was used to detect expression of hMLH1 protein. The relationship between hMLH1 expression with clinical parameters, such as gender, age, cancer differentiation level, infiltration depth, tumor stage, lymphatic metastasis, was analyzed. Results: The positive rates of hMLH1 protein in esophagus squamous cell carcinoma, and atypical hyperplasia tissue and normal esophagus tissue were 36.96%,56.52%, and 84.78%,respectively,with the former 2 significantly lower than the latter (P