OBJECTIVE To explore the problems of infection control in grass-roots hospitals and improving measures.METHODS By further investigation,there were the 8 major problems existing in grass-roots hospitals for hospital infection management,and the 5 corresponding measures and countermeasures.RESULTS Aiming at the problems,to adopt from them the corresponding measures and countermeasures would effectively control the occurrence of hospital infection in grass-roots hospitals.CONCLUSIONS The management of infection control in grass-roots hospitals will be strengthened,and it can be done better if we take into account.

Objective To study the expression of Yes-associated protein(YAP) in endometrial cancer and its clinical pathological significance.Methods Immunohistochemieal MaxVisionTM method was used to detect the expression of YAP in 289 cases of endometrial cancer, and its relationship with clinical pathological features and prognosis were analyzed.Results The expression rate of YAP in type Ⅱ endometrial cancer was higher than that in typeⅠendometrial cancer (87.0％ vs.55.1％, x2 =16.340, P ＜ 0.001).In typeⅠendometrial cancer, the expression rate of YAP in G1 cases was lower than that in G2-G3 cases (51.6％ vs.80.0％, x2 =8.549, P =0.005), not associated with menopausal status (x2 =0.045, P =0.831), FIGO stage (x2 =0.976, P =0.323), lymph vascular space invasion (x2 =0.000, P =1.000), lymph node metastasis (x2 =0.976, P =0.323) and ER status (x2 =3.318, P =0.069).In typeⅡendometrial cancer, the expression of YAP was not significantly associated with menopausal status (x2 =0.305, P =0.581), FIGO stage (x2 =2.395, P =0.122), lymph vascular space invasion (x2 =1.441, P =0.230), lymph node metastasis (x2 =1.351, P =0.245) and ER status (x2 =0.000, P =1.000).Log-rank test analysis showed that YAP high-expression was associated with a significantly worse overall survival in typeⅠ endometrial cancer (x2 =14.023, P ＜ 0.001), but YAP expression was not an independent prognostic factor.Conclusion YAP shows high expression in endometrial cancer.It is associated with histological grade and prognosis of typeⅠ endometrial cancer.

Objective To explore the effect of health education on medication compliance of middle-aged and elderly hypertensive patients in rural areas. Methods Outpatients aged 45 hypertensive patients were divided into two groups, the intervention group was given health education knowledge, intervention of bad habits and knowledge of drug by oral presentations, banner ads, TV screen publicity, publicity, newspaper, telephone follow-up and other forms of propaganda. Results Hypertension knowledge, dietary knowledge, living habits, medication knowledge, medication compliance of patients in the intervention group were increased significantly, and there were significant difference in the four survey in the observation group with statistical significance(P<0.05). Conclusion Health education has a great influence on drug compliance among middle-aged and elderly hypertensive patients in rural areas, and has great significance for controlling blood pressure.

Objective To study the effect of systematic prenatal education on the outcome of childbirth. Methods Pregnant women (200 cases) were randomly divided into 2 groups, 100 pregnant women who were willing to attend the maternity club to accept the systematic prenatal education were set as the experimental group, while 100 pregnant women who only accepted the regular prenatal health education as the control group. The experimental group not only received regular prenatal health education, but also had classes about prenatal gymnastics training exercise, the simulant delivery process, Lamaze pain relieving delivery, various postures of 3 delivery processes, the knowledge of husband assistance, the physical and psychological change and relevant coping method during gestation and postpartum, etc. The control group only accepted the regular prenatal health education, which was watching breastfecding VCD, having classes about the basic health knowledge and the physical process of pregnant period, delivery period, and puerperal period, the breasffecding knowledge, etc. Results The cesarean section rate and the total stage of labor of the experimental group was significantly less and shorter than that of the control group(P<0.05 and P<0.01). Conclusions The systematic prenatal e-ducation can improve pregnant women's knowledge of pregnancy and parturition, improve pregnant women's confidence of natural delivery, help to regulate nerve and the function of different organs, shorten the childbirth process, and reduce the cesarean section rate.

Clinical education for undergraduates in China can be divided into three phases:preclinical phase,clinical clerkship phase,clinical practice phase,and each phase has its own features and different teaching tasks.Currently there are four teaching methods mostly applied in clinical education:lecture-based Learning (LBL),team-based learning (TBL),case-based learning (CBL) and problem-based learning (PBL).LBL is a teacher-centered.teaching method,which focuses on making knowledge accurate,systematic and comprehensive;TBL,CBL and PBL are student-centered teaching methods,which can improve students' enthusiasm and subject initiative.Each teaching method has advantages and dis-advantages.According to the different characteristics of three stages of medical education,this paper analyzes the reasonable application of different medical teaching methods in three stages of undergraduate medical education:in preclinical stage,combining LBL with TBL teaching methods;in clinical clerkship phase,combining the teaching methods of LBL,TBL and CBL;in clinical practice stage,combining CBL with PBL teaching methods.

The character of the membrane of fiber can be simulated by nonpower RC network on condition that the offset of distance between the transmembrane potential of unmyelinated nerve fiber and the resting potential of nerve fiber cell is small enough. Thus, it is possible to found the successive cable equation with external stimulation under subthreshold linear state. So far, most scholars use time-domain analysis method. In this paper are reported the method of integral transform and the analytical expression of cable equation under the stimulation of point electrical source firstly. And at the same time, computer-aided simulation of transmembrane potential of nerve fiber and analysis of the possibility of activating action potential are presented.
Action Potentials ; physiology ; Computer Simulation ; Electric Stimulation ; Humans ; Membrane Potentials ; physiology ; Models, Neurological ; Nerve Fibers, Unmyelinated ; physiology

Objective Using an adenoviral vector , the wild-type PTEN gene was transduced into activated hepatic stellate cell (HSC) in vitro and the phosphorylation status of Akt were investigated. Methods The wild type PTEN gene was transduced into activated HSC in vitro mediated by adenoviral vector. The expressions of PTEN and total Akt in HSC were measured by Western blot and Real-time fluorescent quantitation PCR. And the expressions of phosphorylated Akt (Thr308) in HSC was determined by Western blot. Results The data showed that exogenous wild type PTEN gene was successfully transduced and expressed in activated HSC in vitro. The over-expression of wild type PTEN resulted in the significant down-regulated expression of phosphorylated Akt (Thr308) in activated HSC (P < 0.01). But no significant defferences were found in the expression of total Akt in activated HSC at both transcriptional and translational levels(P>0.50). Conclusions The overexpression of wild-type PTEN can negatively regulate PI3K/Akt signaling transduction by inhibiting the phosphorylation of Akt in activated HSC in vitro.

Objective To investigate the adverse effect of different doses of high power microwave(HPM) irradiation on oxidative stress in the brain of Wistar rats in order to contribute to establishing an animal model to evaluate protective agents which will be used for protection against microwave radiation.Methods Eighty male Wistar rats were randomly divided into 16 groups according to factor analysis.The average power density was 0,10,30 and 100 mW/cm2 and the sampling time was 6 h,1,3 and 7 d .The duration of exposure was 6 minutes for each radiation group.After exposure, the rats were sacrificed at each sampling time.Colorimetric method was used to measure the content of malondialdehyde(MDA) and protein carbonyl, the activity of GSH-px, SOD and CAT.Results The content of MDA and protein carbonyl of each radiation group was increased with the radiation dose, but decreased with the sampling time prolonged.The activity of superoxide dismutast(SOD),glutathion peroxidase(GSH-px) and catalase(CAT) in each radiation group was decreased with the radiation dose increased, and with the sampling time prolonged, but increased later.Conclusion Microwave radiation can cause oxidative stress in rats brain, as shown by the oxidative damage of lipid and protein and the decrease in the activity of antioxidant enzymes.Besides, the effect also depends on the radiation dose and sampling time.

Objective To explore the application effects of blending learning mode which is based on SPOC in preventive medicine teaching. Methods 92 students of Grade 2013 majoring in medical imag-ing were divided into two classes. 47 students in the first class adopted LBL teaching method. Another class was experimental group, teaching the remaining 45 students by blending learning mode based on SPOC method. Analyses and assessments were done by course examinations and questionnaires. Results The experimental groups were better than the control groups not only in the average score of synthetic scores (t=5.068, P=0.000) but also in the 80-89 and 90-100 number distribution (Z=3.713, P=0.000). The question-naires showed that 72.6％students of the experimental group accepted blending learning mode which was based on SPOC. Conclusion Ability of explorative thinking, independent learning and effect of teaching can be achieved by blending learning model which is based on SPOC.

Objective To investigate the detection rate,epidemiology and resistance mechanism of methicil-lin-resistant Staphylococcus aureus(MRSA)in a hospital in recent 5 years.Methods A total of 762 strains of non repetitive Staphylococcus aureus detected from 2016 to 2020 in a hospital were collected retrospectively.Methicillin-sensitive Staphylococcus aureus(MSSA)was 392 strains(MSSA group)and 370 strains caused by MRSA(MRSA group),and 95 strains of MRSA isolated in 2020 were further used for resistance mechanism.Staphylococcus aureus was identified and tested for drug sensitivity by Vitek 2 automatic microbial system.Molecular epidemiological typing was determined by multilocus sequence typing.The biofilm formation was performed by crystal violet staining.PCR amplification was used to detect drug resistance genes,virulence genes and biofilm related genes,and logistic regression analysis was used to investigate the independent risk factors of its occurrence.Results The detection rate of MRSA in past five years was 48.56％,mainly was from pus samples and secretion samples(38.38％,33.51％respectively).MRSA was found in the general sur-gery(18.65％)and otorhinolaryngology(12.70％).ST88 was the most common multilocus sequence typing(37.89％),and followed by ST951(24.21％).Moderate biofilm formation was the most common,accounting for 74.73％.Multivariate regression analysis showed that compared with MSSA group,hypoproteinemia,en-docrine system diseases,wound infection and history of antibiotic use within six months were the independent risk factors for infection in MRSA group.Compared with the control group,hospital transfer,wound infection and tumor were independent risk factors for infection in MRSA group(P＜0.05).Conclusion The detection rate of MRSA in a hospital is high,and the carrying rate of various drug-resistant genes is high.The hospital should pay attention to the prevalence of MRSA and related risk factors,so as to prevent it early.