Objective To investigate the effect of umbilical cord blood monocytes(UCBMC) transplantation in neonatal rats with hypoxic-ischemic brain damage (HIBD) on rat astrocyte proliferation and its correlation with bone morphogenetic protein(BMP) 4.Methods Forty 7-day-old SD rats with the random number table,were divided into normal control(CON) group,hypoxic-ischemic(HI) group,normal(N) + UCBMC group,HI + UCBMC group,10 rats in each group.HIBD model was prepared according to the Rice method.Twenty-four hours after hypoxia,the UCBMC group and HI + UCBMC group were injected with 3 × 106 UCBMC via the lateral ventricle.Seven days after transplantation,changes in the number of neurons were observed by Nissl staining;the expression of glial fibrillary acidic protein (GFAP) was observed by Western blot;the astrocyte proliferation was observed by proliferating cell nuclear antigen (PCNA)/GFAP,BMP4/GFAP immunofluorescence double staining,and their correlation was analyzed.Results Nissl staining showed that the neurons at cerebral cortex and hippocamp were irregularly arranged and decreased in the HI group,and the number of Nissl-stained cells were significantly less than that in the CON group(tcortex =26.54,thippocamp =32.26,all P ＜0.05) ;but the Nissl-stained cells were well arranged in the HI + UCBMC group and more Nissl-stained cells were observed as compared with the HI group (tcortex =10.18,thippocamp =12.56,all P ＜ 0.05) ; Western blot showed that the expression of GFAP in the HI group was significantly higher than that in CON group(t =5.50,P ＜ 0.05) ;but the expression of GFAP in the HI + UCBMC group was significantly lower than that in the HI group (t =3.04,P ＜ 0.05) ; immunofluorescence double staining showed that there were more PCNA + GFAP + cells in the HI group compared with the CON group(t =10.39,P ＜ 0.05),but fewer PCNA + GFAP + cells were observed in the HI + UCBMC group than those in the HI group(t =3.72,P ＜ 0.05).And there were more BMP4 + cells in the HI group compared with the CON group (t =5.52,P ＜ 0.05),but fewer BMP4 + cells were observed in the HI + UCBMC group than those in the HI group(t =2.33,P ＜0.05).The fluorescence intensity of GFAP were correlated with that of BMP4 in HI + UCBMC group (r =0.84,P ＜ 0.05).Conclusions UCBMC transplantation can decrease the proliferation of the astrocytes,thus promote brain damage repair and its mechanism might be collected with the decreased expression of BMP4.

The purpose of this research was to understand fecal micro-flora of Enterococcus-related diarrhea feces and the clone characteristics of isolated Enterococcus strains.Primer was designed according to 16S rRNA gene and integrated DNA extracted from patients' fecal samples was used as the template to amplify the conserved sequence of 16S rRNA by PCR.After the PCR product was purified and cloned into T vector to sequence,PFGE was used to analyze 20 out of 50 Enterococcus isolates from each specimen.The 16S rRNA gene sequencing analysis indicated that these 4 portions of diarrhea feces were dominated by Enterococcus faecium (>68%) and the isolates were clonal except only one portion.Although the basic flora characteristics of diarrhea feces could be revealed by 16S rRNA gene sequencing analysis and PFGE analysis,the pathogenicity and mechanism of Enterococcus were still waiting for further experimental exploration.

Marine Chinese Medicine (MCM) is one of the important part of the traditional Chinese medicine.The exploration of marine organism resources provide a good base for the development of MCM.However,the evaluation for the nature of the new source of MCM becomes one of the key problem of the clinic application of MCM.In this study,613 MCM and their related 1 091 species of marine organisms were screened.Association Rules Mining method and Phylogenetic Tree constructing method were used to find out the association relationship and the distribution regularity of the MCM with different nature in the Phylogenetic Tree.The results showed high collection of the MCM with the same nature on the tree.The MCM from the organisms in the same family might have the same nature.And the association rules for the same nature assembled at the same branch or near branch of the tree.For example,the marine plantae,Chlorophyta,Florideophyceae,and Phaeohpyceae,were related with cold nature,while the marine animalia,including Decapoda,Malacostraca,and Arthropoda,had close relationship with hot nature.Moreover,the neutral nature was related with Squamata.These results implied that there were close relationship between the nature and the affinity relationship.The MCM from the same or close families may have the same or likely nature.This study provided a new index and reference for prediction and evaluation of the nature of new MCM.

An ultrasensitive electrochemical immunosensor modified with graphene and dienestrol(DE)was developed for the detection of dienestrol through indirect competition with K3Fe(CN)6 acting as the redox probes. The results revealed that under optimized conditions a calibration for DE was obtained with a linear range of 500-5 000 ng/mL and the detection limit was up to 0. 2 ng/mL, showing that the proposed electrochemical immunosensor had excellent sensitivity and wide detection range. The immunosensor was examined in real samples for the analysis of DE. A good recovery in the range of 83. 8%-97. 7% was obtained in pork samples.

Objective To explore the effectiveness of impulse oscillometry(IOS)in airway responsiveness measurement and to find out the positive threshold of IOS for asthma diagnosis. Methods Seventy-nine children aged 6-14 years who had suspicious asthma,were recruited into the study. The positive criteria of the methacholine bron-chial provocation test was a 20％ reduction in forced expiratory volume in the first second (FEV 1 )compared to base-line. Simultaneously measured changes in various parameters of IOS,including resonant frequency(Fres),impedance at 5 Hz(Zrs),resistances at 5 and 20 Hz(R5,R20),reactance at 5 Hz(X5),and area of reactance(AX). The results of the challenge test were divided into positive and negative groups according to the pulmonary ventilation function me-thod. The differences between the 2 groups of IOS parameters before and after the challenge test,and the correlation be-tween the change rate of FEV1 and the change rate of IOS parameters were compared,and the positive judgment criteria of IOS parameters in the determination of respiratory responsiveness were determined. Results The positive group of bronchial provocation test had 37 patients and negative group had 42 patients. There was no significant difference in the basic values of parameters between the positive group and the negative group (all P > 0. 05). Changes in Zrs,R5,X5 of IOS were correlated with changes in FEV1 (r = 0. 374,0. 310,0. 449,all P < 0. 05). By single factor analysis,the area under the receiver operating characteristic(ROC)carve (AUC)showed:basic value of Zrs increased by 45. 85％,R5 increased by 45. 72％,X5 increased by 80. 74％ respectively compared to the baseline showed the optimal combination of sensitivity and specificity. In multivariate Logistic regression models,when Zrs and R5 were combined to measure the airway responsiveness,the sensitivity and specificity were 73. 0％ and 81. 0％,respectively. Conclusions IOS and spirometry can be used to determine airway responsiveness in children during methacholine bronchial challenge. Zrs≥45. 85％,or R5≥45. 72％,or X5≥80. 74％,or Zrs and R5 of multiple regression formula can be used as the positive criteria for young children with airway heperresponsiveness,the combination of Zrs and R5 has higher sensitivity and specificity.

Objective:To identify the Key genes in the development of sepsis through weighted gene co-expression network analysis (WGCNA).Methods:The gene expression dataset GSE154918 was downloaded from the public database Gene Expression Omnibus (GEO) database, which containes data from 105 microarrays of 40 control cases, 12 cases of asymptomatic infection, 39 cases of sepsis, and 14 cases of follow-up sepsis. The R software was used to screen out differentially expressed genes (DEG) in sepsis, and the distributed access view integrated database (DAVID), search tool for retrieval of interacting neighbouring genes (STRING) and visualization software Cytoscape were used to perform gene function and pathway enrichment analysis, Protein-protein interaction (PPI) network analysis and key gene analysis to screen out the key genes in the development of sepsis.Results:Forty-six candidate genes were obtained by WGCNA and combined with DEG expression analysis, and these 46 genes were analyzed by gene ontology (GO) and Kyoto City Encyclopedia of Genes and Genomes (KEGG) pathway enrichment to obtain gene functions and involved signaling pathways. The PPI network was further constructed using the STRING database, and 5 key genes were selected by the PPI network visualization software Cytoscape, including the mast cell expressed membrane protein 1 gene (MCEMP1), the S100 calcium-binding protein A12 gene (S100A12), the adipokine resistance factor gene (RETN), the c-type lectin structural domain family 4 member gene (CLEC4D), and peroxisome proliferator-activated receptor gene (PPARG), and differential expression analysis of each of these 5 genes showed that the expression levels of the above 5 genes were significantly upregulated in sepsis patients compared with healthy controls.Conclusion:In this study, 5 key genes related to sepsis were screened by constructing WGCNA method, which may be potential candidate targets related to sepsis diagnosis and treatment.

OBJECTIVE: To isolate the collagen phagocytic subpopulation of fibroblast (CPSF) and non-collagen phagocytic subpopulation of fibroblast (nCPSF) and to identify their differentially expressed genes.
 METHODS: The CPSF and nCPSF was isolated by using collagen-fluorescein-isothiocynate-latex bead (COL-FITC-LB) phagocytosis technique and FCM sorting method. Microarray analysis was used to screen the differentially expressed genes, which were verified by real-time PCR. 
 RESULTS: CPSF and nCPSF was successfully isolated. Seventeen differentially expressed genes were identified. Compared with nCPSF, the expression of 12 or 5 genes was up-regulated or down-regulated in CPSF. Three of the 12 up-regulated genes were urokinase plasminogen activator receptor-associated protein (uPARAP), cytochrome b-245, beta polypeptide (CYBB) and Hook homolog 1 (HOOK1), which were confirmed by real-time PCR. uPARAP mRNA expression level in CPSF was 2788 times of that in nCPSF. CYBB mRNA expression in CPSF was only 0.85 times of that in nCPSF. HOOK1 mRNA expression in CPSF was 1.96 times of that in nCPSF (P<0.05). 
 CONCLUSION A novel method is successfully established to isolate CPSF and nCPSF. uPARAP is the main differentially expressed gene in CPSF and nCPSF, which is obviously involved in the fibroblast collagen phagocytosis. It might be a potential biomarker for treatment of collagen diseases.
Collagen ; genetics ; Down-Regulation ; Fibroblasts ; cytology ; Humans ; Microarray Analysis ; Phagocytosis ; Up-Regulation