Objective:To clone and express the HCDH gene fragment in E.coli,and then to prepare its antiserum.Methods:The HCDH gene fragment was amplified by PCR,and ligated to the expression vector pMAL-P2X after DNA sequencing analysis.Recombinant plasmid was transformed into the E.coli.Then its expression was induced by IPTG and analyzed by SDS-PAGE.The otter rabbit was immunized with maltose resin affinity purified antigen.Antiserum valence was detected using ELISA and AGP test.The specificity was detected using Western blot.Results:The objective products of 786 bp were obtained,then the prokaryotic expression vector of HCDH gene was established and the corresponding antiserum was prepared successfully.Conclusion:It lays solid foundation for the further researches on detecting the changes of HCDH protein on the expression level or the expression regulation of HCDH gene.

The results of detection of circulating antigen(CAg) in cerebrospinal fluids(CSF) of 231 cerebral cysticercosis patients with McAb(4F8)-based ELISA were reported and compared with the case histories , clinical manifestations and CT scans. No relationship was found between CAg detected in CSF of these cases and history of taeniasis. However, the positive rate of CAg in cerebral cysticercosis patients with subcutaneous nodules was found significantly higher than those in cases with simple cerebral cysticercosis and in cases with subcutaneous nodules disappeared after anti-Cysticercus therapy before CSF collection. The results of CAg detection in CSF were related with the CT findings as well as the stage of the disease. It is thus indicated that MeAb(4F8)-based ELISA might be useful not only for diagnosis of neurocysticercosis, but also for the evaluation of efficacy of anti-Cysticercus therapy.

Objective: To study the characteristics of suicides treated in the emergency department of a large general hospital in China, so as to provide relevant data for preventing suicide. Methods:Study the records of 183 suicides in the emergency department of XuanWu hospital during Jan 1st to Dec 31 of 2004. Result:Among all suicide cases, young people (aged 20 to 29 years) were the most (81cases, 44.3%); Females (n=147) outnumbered males (n=36, f:m=4.1:1); 170 (92.8%) cases had ingested poisons, among them, 119 used hypnotics or pesticides. The mortality was 3.3% (6/183), that of males (3/36=8.33%) was much higher than that of females (3/147=2.04%); Most of suicides happened during 18 to 24 o'clock(71cases, 38.8%)(P

Objective To assess the value of transrectal echography(TRE) in diagnosing peri-rectal lesions. Methods The echography images of 287 cases of peri-rectal lesions were retrospectively analyzed,and contrasted with the results of postoperative pathologic examination and the final clinical diagnosis. Results 83 cases of peri-rectal recess lesions were found by TRE, and the occurate rate of diagnosis was 91%. 123 cases of peri-rectal viscera lesions were found by TRE, and the accurate rate of diagnosis was 94%. Conclusion TRE plays an role in the diagnosis of peri-rectal recess and viscera lesions, and in the dynamic and real-time detection of the obstructive causes of bladder orifice.

Objective: To compare the effects of bare-handed cardiopulmonary resuscitation (CPR) and cardiopulmonary resuscitator in emergency treatment. Methods: A total of 185 patients received CPR in our hospital from 2013-01 to 2015-10 were enrolled. The patients were divided into 2 groups: Bare-handed CPR (Bare-handed ) group, n=101 and Cardiopulmonary resuscitator (Mechanical) group, n=84;meanwhile, taking 30 min as border time, each group was further divided as short time CPR subgroup and long time CPR subgroup. High quality CPR was conducted in all patients. The success rates, time and blood levels of lactic acid after CPR were compared among short time subgroup, long time subgroup between Bare-handed group and Mechanical group respectively. Results: For short time CPR patients, the success rates, time and blood levels of lactic acid after CPR were similar between Bare-handed group and Mechanical group; for long term CPR patients, the success rates and time were similar between Bare-handed group and Mechanical group, while blood levels of lactic acid after CPR in Mechanical group (6.88 ± 1.82) mmol/L was lower than Bare-handed group (9.39 ± 2.39) mmol/L, P<0.05. Conclusion: The success rates of bare-handed CPR and mechanical CPR were similar for emergency treatment, both methods were effective; while mechanical CPR may save the manual labor, achieve better metabolic result which should be recommended in clinical practice.

Objective To study the prognostic value of modified early warning score (MEWS) and Acute Physiology and Chronic Health Evaluation (APACHE Ⅱ) score in emergency ICU elderly patients.Methods 113 elderly patients were collected in emergency ICU,after admission to calculate the MEWS score and APACHE Ⅱ score,the MEWS score and APACHE Ⅱ score difference were compared between death group and survival group.the differences were compared in MEWS score ≤ 3,4 ～6,7 ～ 9,≥ 10 scores groups respectively;analysis of the MEWS score and APACHE Ⅱ the relevance score.the correlation analysis of the MEWS score and APACHE Ⅱ score was given.Results The MEWS(7.91 ± 2.42) point and APACHE Ⅱ (21.9 ± 4.18) point in death group were greater than that in survival group [(4.51 ± 2.14) point,(19.53 ± 4.37) point] (t =7.49,2.70,all P ＜ 0.01).With MEWS score increasing,the fatality rate rose(P ＜0.01).the MEWS score and APACHE Ⅱ score was correlated(r =0.617,P ＜ 0.01).Conclusion The MEWS score was a simple and quick scoring system,as well as APACHE Ⅱ,it can predict the prognosis of elderly patients in emergency department,it has important application value.

Objective To study the effect of case-based learning(CBL) combined with problem-based learning (PBL) in emergency teaching for foreign students.Methods Totally 33 foreign students were selected in department of emergency,Xuanwu Hospital of Capital University of Medical Sciences and were randomly divided into the observation group (n =17) and control group (n =16).Students in observation group were taught by CBL combined with PBL while those in control group were taught by traditional teaching method.Theory examination,physical examination and Sirm4000 simulated cardiopulmonary resuscitation skill appraisal were given at the end of the internship and achievement differences were compared between 2 groups.Attitude on the teaching method was studied in observation group by questionnaire.SPSS 17.0 software was used for statistical analysis； measurement data were expressed as mean standard deviation ((x) ± s) ； independent sample t-test was applied for comparison between groups； P ＜ 0.05 stands for difference having statistical significances.Results Theory result was (83.5±7.9) in observation group and (76.6 ±8.7) in control group (P=0.012).Cardiopulmonary resuscitation skill score was (84.3 ± 10.1) in observation group and (75.6-± 11.3) in control group (P =0.013).100.0％ students thought that the teaching method could improve the ability of self-learning.Conclusions Teaching method of CBL combined with PBL can stimulate the students' interest in learning and improve students' achievement.

Objective To investigate the changes of thyroid hormone levels and its relationship with prognosis in old patients with sepsis. Methods APACHE Ⅱ score and SIRS score were documented in 140 old patients with sepsis,who were divided into Multiorgan dysfunction syndrome (MODS) group and non MODS group. Results The levels of FT3 and FT4 descended obviously and APACHE Ⅱ score and SIRS score increased remarkably in old patients with severe sepsis(P <0.05 or P <0.01). The level of FT3 and FT4 in death group and MODS group was much lower than that in the survival group (P < 0.05). Conclusion Inflammatory resction and eytokines may effect the hypothalamic- pituitary-thyroid axis. The levels of thyroid hormone can reflect old patients with sepsis condition. The patients with severer conditions may have lower levels of thyroid hormone who will be more likely to died of MODS.

Objective To study effect and significance of systemic inflammatory response syndrome(SIRS) score and APACHE Ⅱ score in prognosis in severe patients of emergency department.Methods Data of 114 patients were collected at admission.SIRS score and APACHEⅡ score were calculated in 24 hours.To analyze mortality of patients in different score,to estimate dependability of them.Result With SIRS score increasing,mortality rate increased as well and patients with SIRS score ≥2 or APACHE Ⅱ score ≥25 had significantly higher mortality rate.Conclusions SIRS score is a simple,useful predictor of outcome in patients of emergency department.It can be used in clinical generally.

Objective To study teaching method in emergency education of 7-year medical students during clinical practice. Methods 7-year program students during clinical practice were taught by PBL combined with computer simulation teaching and the teaching contents were acute coronary syndrome,upper gastrointestinal bleeding and cardiopulmonary resuscitation. The outcomes of theoretic examination,technique examination and teaching effect investigation were analyzed. Results After clinical practice,students’grades in acute coronary syndrome,upper gastrointestinal bleeding and cardiopulmonary resuscitation were improved obviously(P