Objective To study the relationship between uterine receptivity and estrogen receptor, progesterone receptor on endometrial epithelial cells of LPD. Methods Integrinβ3 subunit, ER,PR on endometrial epithelial cells of LPD swere datermined by immunmohisto chemical analysis. Results Integ rin β3 subunit was significantly decreased, while estrogen receptor and progesterone receptor were significantly elevated in glandular epithelial cells from tissue samples with LPD. Concluslon Uterine receptivity is decreasedin women with LPD, which is closely assosciated with failure of ER、PR downregulation in middle luteal phase.

Objective To explore relationship of CD44 and transforming growth factor-? 1 (TGF-? 1) in endometriosis (EM) patients. Methods The histopathological changes and the expressions of CD44 and TGF-? 1 were observed and determined by immunohistochemistry in 17 cases of ectopic endometrium of EM patients and 17 cases of ovarian ectopic endometrium of EM patients. The other 20 cases of non-endometriosis and non-tumorous endometrial tissues were used as the control group. Results No difference in the expressions of CD44 and TGF-? 1 was found in the ectopic endometrium of EM patients and the control group, but the expressions in the ovarian ectopic endometrium of EM patients were significantly higher than those in the control group. Conclusion The abnormal expressions of CD44 and TGF-? 1 in ectopic endometrium of EM patients may be associated with the genesis and progression of EM.

Objective To detect the protein expression of gonadotropin-releasing hormone (GnRH) and its receptor (GnRH-R) in various endometrial tissues, and to investigate its significance. Methods Immunohistochemistry was employed to analyse the protein expression and localization of GnRH and GnRH-R from endometriosis(EMS), endometrial hyperplasia, endometrial carcinoma and control group. Results GnRH and GnRH-R are expressed at the protein level in all eutopic endometrium, in 50% ectopic endometrium and 92.3% endometrial hyperplasia, while the expressional rate in endometrial carcinoma are 58.8% and 82.4%, respectively. The majority of GnRH and GnRH-R existed in the plasma of glandular epithelium cells and some stroma cells. Conclusions There exists GnRH and its receptor proteins in endometrial tissues, and their abundant expression in endometriosis, endometrial hyperplasia and in endometrial carcinoma may imply that they could be used in clinical diagnosis and treatment.

Objective To investigate the effects of nordihydroguaiaretic acid (NDGA) on the ovary in rats. Methods Forty female SD rats in normal estrous cycles were randomly divided into PBS group and NDGA group, subcutaneously injected of PBS solution and 3% NDGA at the dose of 30 mg/kg respectively every other day for 16 d. The serum concentrations of follicle stimulating hormone (FSH), luteinizing hormone (LH), estrogen (E 2) and progesterone (P) were measured by chemiluminoimmunoassay before and after administration and the mating was confirmed by the occurrence of vaginal plugs and the morphological changes of uterus and ovaries were examined by immunohistochemistry after administration. Results None of the NDGA-treated rats were able to successfully mate as the absence of vaginal plugs were detected. The endometrium could maturate in all of the NDGA-treated rats, but there had a decrease not only in the number of endometrial glands, but also the proliferation rate of the glandular epithelial cells, the endometrial stroma cells, the vessels and the microvacular density (P

Objective To investigate the effects of nordihydroguaiaretic acid (NDGA) on the embryos growth and decidual development in rats. Methods Fifty pregnant SD rats were randomly divided into NDGA-treated group(N)and control group (P), which were given subcutaneous injection of NDGA or PBS on embryonic day 1(E1), E7 and E14, and then killed on E6 and E9. The number of implantations was calculated, the diameter of the embryos was measured and the numbers and weight of neonates were recorded. The expressions of VEGF, PCNA and FⅧRAg in the deciduas and embryos were detected by immunohistochemistry and then made image analysis. The serum concentrations of progesterone on E9 were measured. Results The number of implantations were decreased with NDGA treatment on E1 (P

Objective To investigate the effects of nordihydroguaiaretic acid(NDGA)on the embryos growth and decidualization of rat by intrauterine perfusion and to investigate the new medication for planned parenthood.Methods Fifty-six pregnant SD rats were randomly divided into NDGA-treated group(N)and PBS-treated control group(P)and blank control group(B).By intubation,NDGA or PBS was perfused into the uterus on embryonic day 1(E1)and day 7(E7).Rats were killed on E9 to calculate the number and measure the diameter of the embryos,as well as the number and weight of litters.The expression of VEGF,PCNA and FⅧRAg in deciduas and placenta were detected by immunohistochemistry and image analysis.The serum concentration of progesterone on E9 was measured.Results In comparison with two control groups,the number and the diameter of embryos after perfusion were decreased with NDGA treatment on E1(P0.05).Conclusion NDGA can inhibit the growth of early embryo and interfere with the progress of decidualization obviously by intrauterine perfusion,but has little effect on the serum concentration of progesterone.

Objective　To explore the changes of ER-IR and the ultra structure in the medial preoptic area, arcuate nuclei of early-aged mice treated with estrogen. Methods　Immunohistochemistry assay and electron microscopy were used in this study. Results　ER-IR in the medical preoptic area and arcuate nuclei were greatly reduced after estrogen was given. The cell nuclei of neurons in these areas migrated towards the side, the nuclear membrane became folded, synapse became richer, and the number of synapse vesicle increased. Conclusion　Estrogen can affect the neuron structure and function through the change of estrogen receptor expression in the medial preoptic area and arcuate nuclei of the hypothalamus cardiovascular center.

Objective To explore the significance of the changes of vaginal lactobacilli in normal pregnant women and those with bacterial vaginosis(BV). Methods A total of 309 pregnant women, including 50 pregnant women with bacterial vaginosis, were randomly selected. Lactobacilli isolated from the vaginas of pregnant women were identified by biochemical methods. The distribution of lactobacilli in the vaginas of pregnant women was observed. The ability to produce H 2O 2 and decrease the culture environment PH among four common lactobacilli in the vaginas of pregnant women was also compared. Results The detectable rate of lactobacilli in vaginas of the normal pregnant women was obviously higher than that of women with bacterial vaginosis. The predominant lactobacilli colonizing in the vaginas of the normal pregnant women were L. crispatus and L. acidophilus , most of which could produce H 2O 2. The frequency of H 2O 2 positive isolation in the vaginas of pregnant women was significantly higher than that of BV. Conclusion The study indicates that H 2O 2 positive lactobacilli plays an important role in the protection against vaginal infections in pregnant women.

Objective To investigate the effects of ursodeoxycholic acid (UDCA), dexamethasone (DEX), and S adenomethionine (SAMe) on the pregnant rats with intrahepatic cholestasis induced by ethinylestradiol (EE). Methods A total of 50 15 day pregnant Wistar rats were randomly divided into five groups: control group, EE group, UDCA group, DEX group, and SAMe group. In EE group, rats were subcutaneously injected with EE (2.5 mg?kg -1 ?d -1 ) for 5 d. In the control group, rats received subcutaneous injection of appropriate volume of propylene glycol (PG) for 5 d. In UDCA, SAMe, and DEX groups, rats were administered with the same dose of EE plus UDCA (100 mg?kg -1 ?d -1 ), SAMe (100 mg?kg -1 ?d -1 ), and DEX (1 mg?kg -1 ?d -1 ) for 5 d, respectively. The serum levels of aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatases (ALP), glycocholic acid (GC), and total bilirubin were measured after cesarean. Morphologic changes of the liver of pregnant rats and the growth and development of the fetal rats were observed. Results The serum levels of ALT, ALP, GC in rats of UDCA group were decreased significantly as compared with those in DEX group ( P

Objective To investigate the prevalence of perimenopausal syndrome and relative risk factors in perimenopausal and postmenopausal women in Chongqing city .Methods Totally 1 680 questionnaires from women with perimenopausal syndrome , all the objects were patients who visited gynecology clinic from January 2013 to June 2014 in Chongqing .Results The average men‐arche age was (14 .71 ± 1 .95) years old ;average menopause age was (47 .22 ± 3 .13) years old in 1 680 perimenopausal women . 93 .51% (1 571/1 680) objects were diagnosed perimenopausal syndrome ,mainly presented to be forgetfulness ,sleep disorder ,irrita‐bility ,palpitation ,headache ,hot flashes ,sweating ,loss of libido ,orgasmic dysfunction ,dyspareunia ,colpoxerosis ,bone ,joint and muscle pain ,paraesthesia of skin ,depression .The risk factors were analyzed ,forgetfulness(P < 0 .01) ,sleep disorder(P < 0 .01) , menopause(P< 0 .01) ,normal sex or not(P< 0 .05) ,the attitude of sex(P< 0 .05) ,and the attitude of menopause(P< 0 .05) .Con‐clusion Most women in the period of perimenopausal have the problem with perimenopausal syndrome .The special service should be provided to perimenopausal women ,such as knowledge about perimenopausal and rational hormone replacement therapy .