Objective To investigate the effects of ursodeoxycholic acid (UDCA), dexamethasone (DEX), and S adenomethionine (SAMe) on the pregnant rats with intrahepatic cholestasis induced by ethinylestradiol (EE). Methods A total of 50 15 day pregnant Wistar rats were randomly divided into five groups: control group, EE group, UDCA group, DEX group, and SAMe group. In EE group, rats were subcutaneously injected with EE (2.5 mg?kg -1 ?d -1 ) for 5 d. In the control group, rats received subcutaneous injection of appropriate volume of propylene glycol (PG) for 5 d. In UDCA, SAMe, and DEX groups, rats were administered with the same dose of EE plus UDCA (100 mg?kg -1 ?d -1 ), SAMe (100 mg?kg -1 ?d -1 ), and DEX (1 mg?kg -1 ?d -1 ) for 5 d, respectively. The serum levels of aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatases (ALP), glycocholic acid (GC), and total bilirubin were measured after cesarean. Morphologic changes of the liver of pregnant rats and the growth and development of the fetal rats were observed. Results The serum levels of ALT, ALP, GC in rats of UDCA group were decreased significantly as compared with those in DEX group ( P

Objective To investigate the accuracy and safety of using normal saline as a medium to guide the catheter lumen to assist the localization of the catheter tip. Methods This study included the patients with hematological malignancies in our fully implantable venous port. We enrolled 105 patients from January 2014 to December 2015 as control group, and 220 patients from January 2016 to June 2017 as the experimental group. The control group used the traditional fully implantable venous port after the chest X slice to determine the location of the catheter tip. The experimental group used the intracavitary electrocardiogram location technology to assist the complete implantable venous port catheter tip positioning catheter, then chest X film. Results No catheterization occurred in all the patients. The rate of catheter placement was 100％. In the experimental group, 179 patients (81.36％) had the best placement of catheter tip (i.e., superior vena cava right atrium junction, CAJ), and 35 patients (52.38％) in the control group had the best placement of catheter tip. The proportion of the catheter tip located in the best position (the superior vena cava auricular commissure and CAJ) in the experimental group was higher than that in the control group (χ2=29.615, P < 0.05). Conclusions By the injection of saline guided endocardial mapping real-time monitoring of totally implantable venous transfusion port catheter tip position, can guide the surgeon to grasp the real-time operational direction of totally implantable venous transfusion port catheter tip, accurately positioning the catheter tip position, improve the surgical success rate, worthy of clinical use.

OBJECTIVETo investigate the clinical efficacy of laparoscopic ventriculoperitoneal shunt with temporary external drainage in the treatment of hydrocephalus.

METHODSFifty-two cases of hydrocephalus randomized into two groups to receive laparoscopic assisted ventriculoperitoneal shunt with temporary external drainage (19 male and 7 female patients) and conventional ventriculoperitoneal shunt (20 male and 6 female patients). The catheterization time in the abdominal cavity, release time of intracranial hypertension, average hospital stay, postoperative pains, and postoperative complications were compared between the two groups.

RESULTSLaparoscopic ventriculoperitoneal shunt with temporary external drainage was performed successfully in all the cases without intraoperative conversion to open surgery. Compared with the conventional ventriculoperitoneal shunt, laparoscopic ventriculoperitoneal shunt with temporary external drainage was associated with significantly shortened catheterization time in the abdominal cavity, release time of intracranial hypertension, and average hospital stay (P<0.01) as well as lowered postoperative pain score at 4, 8, 16, and 24 h after the operation. The pain scores at 48 and 72 h postoperatively were comparable between the two groups. During the follow-up 3 months, the patients receiving laparoscopic ventriculoperitoneal shunt were found to have significantly lower rates of peritoneal end obstruction and abdominal cavity infection than those having conventional shunt (3.8% vs 19.2%, P<0.01; 1.0% vs 23.1%, P<0.01).

CONCLUSIONLaparoscopic ventriculoperitoneal shunt with temporary external drainage is feasible and produces better clinical therapeutic effect for management of hydrocephalus.

Abdominal Cavity ; Adult ; Female ; Humans ; Hydrocephalus ; surgery ; Laparoscopy ; Male ; Treatment Outcome ; Ventriculoperitoneal Shunt ; methods

Objective To investigate the effect of self-prescribed Paizhuo Qinggong Herbal For-mula Granule combined with Misoprostol Tablets on uterine recovery in patients with residual intrau-terine tissue after childbirth.Methods A total of 120 patients with residual intrauterine tissue after childbirth were randomly selected.According to the simple randomization method,60 patients were assigned to control group(n=60)and experimental group(n=60).In the control group,patients were treated with a placebo of traditional Chinese medicine formula granules combined with Misopros-tol Tablets.In the experimental group,patients were treated with self-prescribed Paizhuo Qinggong Herbal Formula Granule combined with Misoprostol Tablets.The clinical efficacy,syndrome score of traditional Chinese medicine,residual area of intrauterine tissue,uterine recovery indicators(sum of three uterine diameters and rate of uterine fundus descent),ultrasound blood flow parameters,matrix alloproteinase-9(MMP-9),tissue inhibitor of metalloproteinase-1(TIMP-1),vaginal bleeding,adverse reactions,and uterine curettage rate were analyzed.Results The total effective rate of treatment in the experimental group was higher than that in the control group(P＜0.05).After 1 week and 2 weeks of treatment,there were statistically significant differences in the syndrome score of traditional Chinese medicine and residual area of intrauterine tissue compared with those before treatment(P＜0.05).The syndrome score of traditional Chinese medicine and residual area of intrauterine tissue in the experimental group were lower than those in the control group after 1 week and 2 weeks of treatment(P＜0.05).There were statistically significant differences in the sum of three uterine di-ameters between the two groups after 1 week and 2 weeks of treatment compared with those before treatment(P＜0.05).The sum of three uterine diameters in the experimental group was lower than that in the control group after 1 week and 2 weeks of treatment(P＜0.05).The rate of uterine fun-dus descent in the experimental group was higher than that in the control group(P＜0.05).There were statistically significant differences in the resistance index(RI),end-diastolic peak blood flow velocity(EDV),peak systolic blood flow velocity(PSV),and pulsatility index(PI)of the uterine spiral artery between the two groups after 1 week and 2 weeks of treatment compared with those be-fore treatment(P＜0.05).The EDV and PSV in the experimental group were lower than those in the control group after 1 week and 2 weeks of treatment,while the RI and PI were higher(P＜0.05).There were statistically significant differences in the serum levels of matrix metalloprotein-ase-9(MMP-9)and tissue inhibitor of metalloproteinase-1(TIMP-1)between the two groups after 1 week and 2 weeks of treatment compared with those before treatment(P＜0.05).The serum levels of MMP-9 and TIMP-1 in the experimental group were lower than those in the control group after 1 week and 2 weeks of treatment(P＜0.05).The duration of vaginal bleeding in the experimental group was shorter than that in the control group,and the amount of vaginal bleeding was better in the experimental group(P＜0.05).The uterine curettage rate in the experimental group was lower than that in the control group(P＜0.05).Conclusion The combination of self-prescribed Paizhuo Qinggong Herbal Formula Granule combined with Misoprostol Tablets is effective and safe in the treatment of patients with residual intrauterine tissue after childbirth.It can improve clinical symp-toms,uterine blood circulation,reduce the residual area of intrauterine tissue,promote uterine re-covery,improve vaginal bleeding,reduce the rate of uterine curettage,and exert its effect by correc-ting the imbalance of MMP-9 and TIMP-1.

Objective To investigate the effect of self-prescribed Paizhuo Qinggong Herbal For-mula Granule combined with Misoprostol Tablets on uterine recovery in patients with residual intrau-terine tissue after childbirth.Methods A total of 120 patients with residual intrauterine tissue after childbirth were randomly selected.According to the simple randomization method,60 patients were assigned to control group(n=60)and experimental group(n=60).In the control group,patients were treated with a placebo of traditional Chinese medicine formula granules combined with Misopros-tol Tablets.In the experimental group,patients were treated with self-prescribed Paizhuo Qinggong Herbal Formula Granule combined with Misoprostol Tablets.The clinical efficacy,syndrome score of traditional Chinese medicine,residual area of intrauterine tissue,uterine recovery indicators(sum of three uterine diameters and rate of uterine fundus descent),ultrasound blood flow parameters,matrix alloproteinase-9(MMP-9),tissue inhibitor of metalloproteinase-1(TIMP-1),vaginal bleeding,adverse reactions,and uterine curettage rate were analyzed.Results The total effective rate of treatment in the experimental group was higher than that in the control group(P＜0.05).After 1 week and 2 weeks of treatment,there were statistically significant differences in the syndrome score of traditional Chinese medicine and residual area of intrauterine tissue compared with those before treatment(P＜0.05).The syndrome score of traditional Chinese medicine and residual area of intrauterine tissue in the experimental group were lower than those in the control group after 1 week and 2 weeks of treatment(P＜0.05).There were statistically significant differences in the sum of three uterine di-ameters between the two groups after 1 week and 2 weeks of treatment compared with those before treatment(P＜0.05).The sum of three uterine diameters in the experimental group was lower than that in the control group after 1 week and 2 weeks of treatment(P＜0.05).The rate of uterine fun-dus descent in the experimental group was higher than that in the control group(P＜0.05).There were statistically significant differences in the resistance index(RI),end-diastolic peak blood flow velocity(EDV),peak systolic blood flow velocity(PSV),and pulsatility index(PI)of the uterine spiral artery between the two groups after 1 week and 2 weeks of treatment compared with those be-fore treatment(P＜0.05).The EDV and PSV in the experimental group were lower than those in the control group after 1 week and 2 weeks of treatment,while the RI and PI were higher(P＜0.05).There were statistically significant differences in the serum levels of matrix metalloprotein-ase-9(MMP-9)and tissue inhibitor of metalloproteinase-1(TIMP-1)between the two groups after 1 week and 2 weeks of treatment compared with those before treatment(P＜0.05).The serum levels of MMP-9 and TIMP-1 in the experimental group were lower than those in the control group after 1 week and 2 weeks of treatment(P＜0.05).The duration of vaginal bleeding in the experimental group was shorter than that in the control group,and the amount of vaginal bleeding was better in the experimental group(P＜0.05).The uterine curettage rate in the experimental group was lower than that in the control group(P＜0.05).Conclusion The combination of self-prescribed Paizhuo Qinggong Herbal Formula Granule combined with Misoprostol Tablets is effective and safe in the treatment of patients with residual intrauterine tissue after childbirth.It can improve clinical symp-toms,uterine blood circulation,reduce the residual area of intrauterine tissue,promote uterine re-covery,improve vaginal bleeding,reduce the rate of uterine curettage,and exert its effect by correc-ting the imbalance of MMP-9 and TIMP-1.

OBJECTIVETo evaluate the efficacy and safety of meropenem in Chinese patients, we conducted a study for the treatment of patients with lower respiratory tract infections, urinary tract infections and other infections.

METHODSA total of 182 hospitalized patients were enrolled in the study. 90 patients received 500 mg meropenem every 12 hours (or 1 g every 12 hours if necessary) and 92 patients received imipenem/cilastatin 500 mg/500 mg every 12 hours (or 1 g every 12 hours if necessary) by intravenous infusion. The duration of treatment was 7 - 14 days for both groups.

RESULTSSeventy of 90 cases receiving meropenem and 70 of 92 cases receiving imipenem/cilastatin were assessable for clinical efficacy. The overall efficacy rates were 90% for the meropenem group and 87% for the imipenem/cilastatin group, and the bacterial eradication rates were 86% in both groups. 93 (76%) of 123 strains isolated from patients produced beta-lactamases. Adverse drug reactions were evaluated in 72 cases in the meropenem group and 70 cases in the imipenem/cilastatin group. The adverse drug reaction rates were 9.7% and 8.6%, respectively. The results showed that there were no statistical differences between these two groups (P > 0.05).

CONCLUSIONMeropenem is effective and safe for the treatment of bacterial infections caused mainly by beta-lactamase-producing strains.

Adult ; Aged ; Alanine Transaminase ; blood ; Aspartate Aminotransferases ; blood ; Cilastatin ; administration & dosage ; adverse effects ; therapeutic use ; Female ; Humans ; Imipenem ; administration & dosage ; adverse effects ; therapeutic use ; Male ; Middle Aged ; Respiratory Tract Infections ; drug therapy ; Thienamycins ; adverse effects ; therapeutic use ; Urinary Tract Infections ; drug therapy

Myocardial infarction is the most common cause of heart failure,and myocardial remodeling can occur after infarction,thus contributing to the progression of heart failure.The occurrence of post-infarction ventricular remode-ling is closely related to m6A methylation.m6A methylation is a reversible and highly dynamic process.This process is mainly mediated by m6A methylation positive and negative regulatory enzymes and is involved in the occurrence of post-in-farction myocardial remodeling through mechanisms such as cellular autophagy.This article mainly reviews relevant litera-ture in recent years.Firstly,a brief introduction is given to m6A methylation,followed by an introduction to the role of m6A methylase in regulating myocardial remodeling.Finally,a summary analysis is conducted on the mechanism of m6A methylation in regulating myocardial remodeling from the perspectives of autophagy,inflammation,cell apoptosis,calcium ion homeostasis,extracellular matrix remodeling,and ferroptosis.The feasibility of using m6A methylation serological de-tection as a diagnostic tool for myocardial remodeling after myocardial infarction is discussed,in order to provide reference for related research.

OBJECTIVE To screen the potential drug targets and signaling pathways of Acanthopanax senticosus for the treatment of Alzheimer’s disease(AD) by bioinformatics and network pharmacology-based approach, and to preliminarily validate its efficacy. METHODS The ingredients of Acanthopanax senticosus were obtained through literature, the ingredients were screened by Swiss ADME, and potential targets were predicted by Swiss Target Prediction. AD’s differentially expressed genes were screened from the GSE28146 dataset. The target of Acanthopanax senticosus and AD target were mapped to construct a “drug-ingredients-potential target-disease” network and protein-protein interaction network. The DAVID database was used for GO and KEGG enrichment analysis. Autodock software was used to verify the molecular docking between key active ingredients and core targets. AD mice model was induced by D-galactose combined with aluminum chloride. Morris water maze test was performed to examine the learning memory ability of each group of mice and to observe the pathological changes in the hippocampus of mice. RESULTS Screened to obtain 24 active components and 74 potential targets of Acanthopanax senticosus for the treatment of AD. “Drug-ingredients-potential target-disease” network indicated that quercetin and kaempferol were the main components of Acanthopanax senticosus for the treatment of AD, and the protein-protein interaction network indicated that STAT3, MAPK1 and PIK3CA were the key targets. Obtained 366 GO enrichment entries(P<0.01) and 109 KEGG enrichment pathways(P<0.01). It mainly involved PI3K-AKT, AGE-RAGE, TNF and other pathways. The molecular docking results showed that the main active ingredients of Acanthopanax senticosus were able to bind well to the main targets. The in vivo pharmacological results showed that Acanthopanax senticosus could significantly improve the learning and memory ability of mice, reduce hippocampal tissue damage, and decrease the content of TNF-α, IL-6, and IL-1β in hippocampal tissue. CONCLUSION Acanthopanax senticosus may exert anti-AD effects by inhibiting the expression of inflammatory factors and reducing inflammatory damage.