Objective To investigates the gene expression of telomerase by real-time quantitative telomeric-repeat amplification protocol assay (RTQ-TRAP) in the peripheral blood mononuclear cells (PBMC) of colorectal carcinoma patients and the relationship between the telomerase gene expression in PBMC and clinicopathological features. Methods Peripheral blood samptes were collected from 71 colorectal carcinoma patients, 20 benign colorectal disease patients and 25 normal controls. The telomerase gene expression in PBMC was measured by RTQ-TRAP, and serum CEA in colorectal carcinoma patients was measured by chemiluminescence immunoassay. Results The gene expression of telomerase in PBMC was positive in 50 out of 71 cancer patients (70. 4% ) , 1 out of benign patients (5. 0% ), respectively. The difference of the telomerase gene expression of PBMC in benign colorectal diseases and cancer patients was significant (χ2 = 24. 521, P < 0. 001 ). There was no significant association between the expression of telomerase and patient's gender, age, Dukes stage, and tumor site. The positive rate of CEA in colorectal carcinoma patients was not significantly higher than positive rate of telomerase gene expression(χ2 = 2. 286,P = 0. 125). Conclusions The RTQ-TRAP method is highly accurate and sensitive in measuring telomerase gene expression. The detection of telomerase gene expression in PBMC of colorectal carcinoma patients is a simple and useful molecular marker for the diagnosis of colorectal carcinoma.

Sixty general practitioners of in-service training undertaking rotation in gastroenterology department of Qingdao Municipal Hospital from July 2017 to July 2019 were randomized assigned in trial group ( n=30) and control group ( n=30). The problem-oriented mode was applied in trial group and conventional mode was applied in control group for teaching of two typical digestive diseases (upper gastrointestinal bleeding and acute pancreatitis). The formative evaluation and questionnaire survey were used to compare the teaching effects and the results of evaluation were compared with χ 2 test by SPSS 17.0 between two groups. The excellent and good rates of evaluation for the clinical psychological quality, clinical reasoning ability, doctor-patient communication ability and practice-based learning and improvement ability in trial group were significantly higher than those in the control group(χ2=7.38, P=0.03; χ2=12.96, P<0.01; χ2=23.33, P<0.01; χ2=16.14, P<0.01). Questionnaire survey showed more satisfaction towards teaching method in trial group was higher than that in control group(χ2=12.86, P<0.01); and the clinical reasoning ability, learning initiative and self-confidence in trial group were improved more markedly than those in control group(χ2=8.26, P=0.02; χ2=19.48, P<0.01; χ2=21.46, P<0.01). The problem-oriented clinical thinking teaching model demonstrates good effects on clinical comprehensive ability for general practitioners of in-service training during gastroenterology rotation, which is worth further promotion.

Objective:To evaluate the application of digital memory in abdominal physical examination teaching in cultivating gastroenterology professional postgraduates during the course of clinical rotations.Methods:Sixty clinical specialty postgraduates from Qingdao Municipal Hospital practicing in gastroenterology department from September, 2016 to September, 2018 were selected as subjects, and were randomized into experimental group ( n=30) and control group ( n=30). They were respectively received digital memory mode and traditional mode for clinical practice. The teaching effect was evaluated by comparing the scores of theory examination, skill results and clinical assessment at the end of training stage. Statistical analysis was performed using t test and chi-square test by SPSS 17.0. Results:There was no significant difference in the scores of theory examination and skill operation results between the experimental group and the control group, however, the number of excellent postgraduates and the average scores in clinical assessment were significantly higher than those in control group ( χ2=9.14, P=0.01). Questionnaire survey showed good evaluation of digital memory model in the experimental group. The recognition rate of learning interest and initiative, theoretical knowledge, systematic memory, clinical thinking ability as well as operational skills are 56.67%, 63.33%, 66.67%, 63.33% respectively. Conclusion:The digital memory mode in abdominal physical examination teaching demonstrates good effects on cultivating professional postgraduates' clinical comprehensive ability during the course of rotation in gastroenterology department, which worth further popularization.