Objective To study the molecular mechanism of the apoptosis in rat tubular epithelial cells induced by albumin in vitro.Methods The cultured rat renal tubular epithelial cells were incubated with 20 mg/ml delipidated and endotoxin-free bovine serum albumin(BSA) for 2,6,12 and 24 h respectively.The NF-?B activity was assessed by electrophonetic mobility shift assay(EMSA) and the osteopontin(OPN) mRNA expression was analyzed by reverse transcription-polymerase chain reaction(RT-PCR).The cell apoptosis was evaluated by flow cytometry.Results The rate of apoptosis was increased when the cultured rat renal tubular epithelial cells were incubated with 20 mg/ml BSA for 2,6,12 and 24 h respectively.The NF-?B activity and expression of OPN were up-regulated in the cultured rat renal tubular epithelial cells with the treatment of BSA,with significant difference compared with those of the control group(P

Objective To explore whether or not the function of atragalus membranaceus combined fosinopril on decreasing NF- κB activity and OPN expression in overload albumin stimulating proximal tubuler cells. Methods Cultured cells without stimulation were used as placebo. Cultured cells were incubated with bovine serum albumin fat acid free (BSA) 20mg/ml as the control. Cultured cells were incubated with fosinopril for different hours as the treatment. Cultured cells were incubated with atragalus membranaceus combined fosinopril for different hours as the atragalus membranaceus group. ELISA and RT-PCR were used to observe NF -κB activity and OPN expression after cultured for 12h, 24h. Result Atragalus membranaceus combined fosinopril rapidly decreased NFκB activity and OPN expression. Compared with the other groups, the difference was significant. Conclusion Astragalus membranaceus combined fosinopril can obviously decrease NFκB activity and OPN expression in overload albumine stimulating proximal tubular cells.

rotein binding toxin and pathogenic components, and reduce clinical symptoms and patients'prognoses, which is more effective than HP or CVVHDF.