To analyze drug resistance of pseudomonas aeruginosa in patients with mechanical ventilation, drug resistance of pseudomonas aeruginosa in patients with tracheal incision was increased, compared with tracheal cannula. The drug resistance to amikacin, ciprofloxacin, meziocillin, ticarcillin was significantly increased( P

OBJECTIVE To analyze the curative effect and bacterial clearance of meropenemagaint infections.METHODS To analyze 2732 cases with infection from more than 170 hospitals after meropenem.RESULTS The effectiveness ratio on various infections of blood,lungs treatmeot liver,gallbladder,and pancreas systems and on infections after transplantabions was 80.3%,87.1%,63.6% and 73.3%,respectively;the total clinical effectiveness ratio was 83.5%.The bacterial clearance ratio in various infections of blood,lungs liver,gallbladder,and pancreas systems and in infections after transplantations was 33.5%,66.2%,65.5% and 66.7%,respectively;the total bacterial clearance ratio was 52.7%.CONCLUSIONS Meropenem is effective against infection in clinical patients.The total clinical effectiveness ratio is 83.5%.The total bacterial clearance ratio is 52.7%.

OBJECTIVE To analyze the risk factors and antimicrobial resistance pattern of Stenotrophomonas maltophilia isolated from 6 hospitals in Beijing,and study the difference in resistance rate of isolates from different hospitals. METHODS In this study,145 cases with S.maltophilia infection were analyzed and their susceptibility was tested.The synergetic screening test was applied to detect metallo-?-lactamases. RESULTS Most of 145 strains were isolated from sputum(86.2%),mainly from ICU(48.3%),and department of respiratory medicine(22.1%).The drug sensitivity tests in vitro showed these strains were resistant to commonly used antibiotics,and drugs whose sensitive rate was over 50% included doxycycline,gatifloxacin,cefoperazone-sulbactam,levofloxacin,compound sulfamethoxazole,ceftazidime-clavulanate and ticarcillin-clavulanate.The antimicrobial resistance of strains isolated from different wards showed some difference.Of all the infected patients,56.7% had underlying diseases;92.2%were treated previously with broad-spectum antibiotics;76.8% underwent invasive examination or treatment. CONCLUSIONS S.maltophilia is resistant to many kinds of antibiotics.The infection caused by S.maltophilia often occurs in patients with severe underlying disease and low immunity.Antibiotics should by chosen by drug sensitivity tests.

To investigate the changes of inducible nitric oxide synthase mRNA (iNOSmRNA) in pulmonary tissues of tail suspended rats during simulated weightlessness, experiments on 40 Spragno Dawley male rats were conducted. The rats were divided into 4 groups:suspended for 7d, and its control, suspended for 21d, and its control tail suspension(-30 ) was used as weightlessness simulation.The condition of iNOSmRNA in pulmonary tissues was examined by hybridization in situ.The results showed that the level of iNOSmRNA in pulmonary tissues of 7d tail suspended rats increased significantly( P

To investigate the changes of vascular endothelial growth factor mRNA (VEGFmRNA) in pulmonary tissues of tail suspended rats during simulated weightlessness,the experiments were conducted on 40 Spragno Dawley male rats,divided into 4 groups:suspended for 7d,control of 7d and suspended for 21d,control of 21d.Tail suspension(-30?) was used as weightlessness simulation.The condition of VEGFmRNA in pulmonary tissues was examined by hybridization in situ. The results showed that the level of VEGFmRNA in pulmonary tissues of 7d tail suspended rats increased significantly( P

10 years. Pulmonary functions were determined routinely and followed for 10 years. Results Comparison between the values of pulmonary functions at the beginning and end of a 10- year follow-up showed very significant difference in the groups of Ⅲ, Ⅳ and Ⅴ. Among the parameters examined, FEV_1, PEF, MMEF, and MVV showed significant increase in these 3 groups (P

To study changes of lung function for 10 years in the elder patients with chronic obstructive pulmonary diseases (COPD) who have quitted smoking for more than 10 years, 45 elder patients with COPD who had quitted smoking for more than 10 years were selected, and 11 elder patients with COPD who had never smoked were enrolled too. Routine lung function tests were performed. Initial values of VC, FVC, FEV 1 , PEF, MMEF, and MVV were compared with those 10 years later, and it was found the difference was significant ( P

Objective To assess the effects of survivin-specific siRNA on chemosensitivity of lung cancer cell A549 to paclitaxel in vitro.Methods For determining if survivin-specific siRNA could enhance the responsiveness of lung cancer to paclitaxel,human lung adenocarcinoma cell lines A549(p53 wild-type)were divided into four different treatment groups:control,survivin siRNA,paclitaxel and survivin siRNA+paclitaxel.The effects on cell proliferation,cell cycle and apoptosis were analyzed by MTT assay and flow cytometry,respectively.The protein expression levels of survivin,p21 and PARP were evaluated by Western blot experiments.Results Survivin-specific siRNA showed a augmenting effect on the chemosensitivity of different concentrations of paclitaxel(P

Objective To compare the induction of L1 and L2 ?-lactamase stenotraphomonas maltoptilia by common antimicrobial agents, including imipenem, meropenem, cefotaxime and ceftazidime, and to survey the modulation of L1 and L2 ?-lactamase expression. Methods One clinical strain of S. maltophilia was isolated and identified with VITEK automatic microbic system. L1 and L2 ?-lactamase genes were amplified, cloned and sequenced by PCR method. Minimal inhibitory concentrations (MICs) of four antimicrobial agents against the clinical isolates were determined by agar dilution method. Two hours after being induced by different concentrations of four antimicrobial agents, total RNA was extracted, and RT-PCR method was used to determine the induction of L1 and L2 ?-lactamase by different concentrations (0.25, 1 or 4?MIC) of common antimicrobial agents. Electrophoresis strips of L1 and L2 ?-lactamase were quantified by Image J software. Results The clinical isolates of S. maltophilia with simultaneous production of L1 and L2 ?-lactamse were identified. When different concentrations of four antimicrobial agents were used as inductors, electrophoresis strips of L1 and L2 amplicons were not found in strains of blank control and those in which imipenem, meropenem or cefotaxime (4?MIC) was added to the culture mediam, while light electrophoresis strips were exhibited by the isolates with ceftazidime (0.25, 1 or 4?MIC) or cefotaxime (0.25?MIC) added to the medium. The strongest electrophoresis strips and the strongest expression were found in the isolates with cefotaxime (1?MIC) added to the medium. Conclusions Clinical common antimicrobial agents, e.g. ceftazidime and cefotaxime, are able to induce production of L1 and L2 ?-lactamase, and cefotaxime (1?MIC) is the strongest inductor. Cefotaxime can exert an effect on transcription of L1, L2 genes simultaneously, implying that a significant overlap might exist between the mechanism of modulation of two ?-lactamases.

Objective To study the dynamic changes in calcineurin-? expression in lung tissue of rats under simulated weightlessness condition,and the influence of ligustrazine(tetramethylpyrazine) on the changes in its expression.Methods The rat model of simulated weightlessness condition was reproduced by suspending the rat by tail in-30?.Thirty healthy Wistar rats were randomly divided into 3 groups(10 each): control group,suspended group(rat was suspended by tail for 7 days) and ligustrazine group(rat was suspended by tail and ligustrazine was given for 7days).In the ligustrazine group,the ligustrazine was gavaged in a dose of 1.5mg/kg(once a day).The expression of calcineurin-? in lung tissue was determined by immunohisochemistry and Western blotting.Results It was revealed by immunohisochemistry and Western blotting that the level of expression of calcineurin-? in lung tissue of the animals in suspended group was significantly higher that in control group and ligustrazine group(P0.05).Conclusion The level of expression of calcineurin-? in rat lung tissue may be increased obviously under the simulated microgravity condition,while ligustrazine treatment may down-regulate the calcineurin-? expression.