Objective To explore the significance of established Tp0259-PCR in diagnosing primary syphilis.To provide basis for establishing new PCR diagnosis method of Treponema pallidum.Methods Specific primers were designed by the gene sequences of Tp0259 which was obtained from Genbank.Tp0259-PCR was established under the optimization reaction condition.Using the new construction of amplification method to detect the common pathogenic microorganism in urinary and reproductive tract infec-tion.42 clinical secretion specimens suspected primary syphilis were tested by Tp0259-PCR.Serum specimens from the same pa-tients were tested by TPPA.Results Only specific amplicons could be found in amplifying the Treponema pallidum by Tp0259-PCR.The detection rate was 73.8% using Tp0259-PCR which was apparently higher than 57.1% using TPPA(P <0.05 ).The specificity of the established method was 100% comparing with TPPA.Conclusion The established method of Tp0259-PCR has high sensibility and specificity.This method might be better than serological method in diagnosis of primary syphilis.but its clinical diagnosis value need to be repeatedly verified by more clinical specimens.

Objective To establish a method for detection Tp0259 gene by PCR .To provide basis of diagnosis infection of trepo‐nema pallidum by detection Tp0259 gene .Methods Gene sequences of Tp0259 was obtained from Genebank and analysis by bioin‐formatics .Specific primers were designed .The whole genome was extracted from testis specimens of the New Zealand rabbits infec‐ted by treponema pallidum and control groups .The gene of Tp0259 was amplified by PCR under the optimization reaction condi‐tions which was selected by many tests ,using the new construction of amplification method to detect the common pathogenic micro‐organism in urinary and reproductive tract infection .Results Specific bands of 646 bp was appeared only in New Zealand rabbits which was infected by treponema pallidum ,while none was in the control group and other pathogenic microorganisms .Conclusion Detection the Tp0259 gene could be used to diagnose infection of treponema pallidum .

Objective To investigate whether there is a correlation between the esophageal length of the adult Chinese people and their height, sitting height, sex or age. Methods The length from the upper end of esophagus to the dentate line of the cardia was measured by watching esophageal cavity with endoscope. A total of 613 cases (378 males and 235 females) were studied. Results ① The average length of esophagus was (24.8?2.1)cm for male and (22.8?1.9)cm for female. The difference between male and female was statistically significant (P

Objeetive To evaluate the relationship between aspartate aminotransferase to alanine aminotransferase ratio (AST/ALT ratio) and metabolic syndrome in college students.Methods Anthropometric and metabolic measurements including fasting plasma glucose (FPG),triglyceride (TG),high-density lipoprotein cholesterol (HDL-C),true insulin (TI),(AST and ALT) were assessed in a crosssectional study of 425 college students aged 19 to 24 years old (male 216,female 209) in 2009.The participants were then assigned to the AST/ALT ratio ＜ 1 group or the AST/ALT ratio ≥ 1 group.Metabolic syndrome was defined as Adult Treatment Panel Ⅲ criteria.Results AST/ALT ratio ＜ 1 was found in 146 subjects (34.4％).After adjustment for age and sex,AST/ALT ratio showed a positive correlation with HDLC (r=0.125) and negative correlations with body mass index (BMI,r=-0.281),waist circumtance (WC,r =-0.264),TG (r =-0.134),TI (r =-0.118) and HOMA-insulin resistance (HOMA-IR,r =-0.121) (all P ＜0.05).The prevalence of metabolic syndrome was 2.1％ and was similar in males and females (2.3％ vs.1.9％,P =0.774).Those with AST/ALT ratio ＜ 1 had a significantly higher prevalence of metabolic syndrome (4.8％ vs.0.7％,P =0.016).After adjustment for age,gender and BMI,the prevalence of metabolic syndrome of subjects with AST/ALT ratio ＜ 1 was nearly 7 (95％ CI:1.430 to 34.019,P =0.016) times of those with AST/ALT ratio ≥ 1.Conclusion AST/ALT ratio may be related with metabolic syndrome in college students.

Objective To investigate the application of microemulsion thin layer chromatography (TLC) for separation and identification of various active components in Xinnaomaitong capsule .Methods The all-grass of Bluecalyx japanese rabo-dosia,rootof Puerarialobata,rootandrhizomeof Salviamiltiorrhiza,rhizomeof LigusticumchuanxionginXinnaomaitong capsule were separated and identified simultaneously with the polyamide film as stationary phase and the microemulsion as mo-bile phases .Different factors ,such as the sodium dodecyl sulfate (SDS) ,oil phase and water phase which effect on the separa-tion of the components were evaluated .Results The all-grass of Bluecalyx japanese rabodosia ,root of Pueraria lobata ,root andrhizomeof Salviamiltiorrhiza,rhizomeof LigusticumchuanxionginXinnaomaitongcapsulewereseparatedandidentified simultaneously with mobile phase of microemulsion∶formic acid∶acetone (5∶1∶1) .The constituent of microemulsion was SDS∶butyl alcohol∶n-Heptane∶water = 6 .7∶15 .8∶2 .5∶75 .0 (g/g) .The spots were clear ,the negative control was no interference ,and it showed an ideal separation .Conclusion Microemulsion TLC is simple ,accurate ,and reproducible .It can identify a variety of active components in traditional Chinese medicine simultaneously .

10.3969/j.issn.2095-4344.2013.25.017

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Objective To evaluate the effect of sevoflurane preconditioning on autophagy in the operated lung tissues of the patients undergoing one-lung ventilation.Methods Forty patients of both sexes,with American Society of Anesthesiologists physical status Ⅰ-Ⅲ,aged 30-80 yr,weighing 45-84 kg,scheduled for elective radical operation for lung cancer performed via video-assisted thoracoscope under general anesthesia,were divided into control group (C group) and sevoflurane preconditioning group (SP group),with 20 patients in each group.In SP group,sevoflurane was inhaled for 30 min with the final concentration of 2.0％,followed by washout.The normal lung tissues (the distance from the edge of the lump＞2 cm) around the lump were obtained for detection of the expression of microtubule-associated protein 1 light chain 3 Ⅱ by Western blot.Results Compared with C group,the expression of microtubule-associated protein 1 light chain 3 Ⅱ in lung tissues was significantly up-regulated in SP group (P＜0.05).Conclusion Sevoflurane preconditioning can enhance autophagy in the operated lung tissues of the patients undergoing one-lung ventilation,which may be related to the lung protective effect.

Objective:To investigate the induction and differentiation potential of ADSCs by tissue culture method,and to preliminary study on the origin of ADSCs.Methods:Using adipose tissue culture method to culture human ADSCs.The third generation of ADSCs for the adipogenic and osteogenesis differentiation,and staining by oil red O and alizarin red S.HE staining was performed after the seventh day culture of adipose tissue.Results:The primary human ADSCs were successfully cultured with adipose tissue culture method.ADSCs cultured to the eighth generation,still maintained a good proliferation ability and cell morphology.ADSCs can be successfully induced into adipose cells and bone cells.ADSCs were mainly distributed around the mesenchymal vascular and connective tissue,by HE staining of adipose tissue after seven days of culture.Conclusion:The cells that were cultured with adipose tissue have the potential to adipogenic and osteogenesis differentiation.The ADSCs were mainly distributed around the mesenchymal vascular and connective tissue.