Objective To compare the efficiency of χ~2-Fisher's exact test which is one of the competitive null hypothesis approaches with SAM-GS which belongs toself-contained null hypothesis approaches. Methods The two methods were used to analyze a simulation experiment which contained five different scenarios. The results were compared with the simulated initialization,and assessing indexes were calculated to compare the efficiency. Results Under the same conditions,SAM-GS always have higher power than that of χ~2-fisher's exact test. However, the final inference is equivalent, namely if the difference between the two groups are smaller than 0.30,the two methods can not be better to identify differences between them. By contrary, when the differences between the two phenotypes are larger than 0.30, the two ways can both identify differences. Conclusion SAM-GS tends to have slightly higher power thanχ~2-Fisher' s exact test. The two methods can be used for screening enrichment gene sets of gene expression profile.χ~2-Fisher's exact test has the important advantage of being able to analyze multi-class phenotype.

Objective To investigate the Allelic and haplotype frequency distribution of seventeen short tandem repeat loci of Y chromosome in Heiyi Zhuang ethnic groups in Guangxi province.Methods Seventeen Y-STR loci,of which the template DNAs were extracted from blood samples of 184 unrelated male individuals in Heiyi Zhuang population,were amplified by using the AmpFlSTR YfilerTM.The PCR products were genotyped with ABI PRISM 310 genetic analyzer.Results The Gene diversity ranged from 0.4910 to 0.9727 at DYS456、DYS389Ⅰ、DYS390、DYS389Ⅱ、DYS458、DYS19、DYS385a\b、DYS393、DYS391、DYS439、DYS635、DYS392、Y-GATA-H4、DYS437、DYS438、DYS448.A total of 180 different haplotypes were observed,The haplotype diversity value calculated from all 17 loci was 0.99976.The significant difference of the allelic frequency distribution in Y-STR loci was found between Heiyi Zhuang population and other observed populations.Conclusion The 17th Y-STR loci in Heiyi Zhuang population of Guangxi province are highly affluent genetic polymorphic and can offer valuable genetic datas for paternity testing and paternal genetic lineages evolution.

Objective To discuss the improvement of surgical techniques and adjustment of immunosuppressive regimen for combined liver and intestinal transplantation.Method A male patient with liver dysfunction and short bowel syndrome underwent the combined liver and intestinal transplantation.Ostomy of graft was performed instead of intestinal anastomosis during the operation.The anastomosis of graft and autologous intestine was performed 8 months after transplantation.Hospital and follow-up data of the patients were analyzed retrospectively.Result The functions of liver and small bowel recovered smoothly after operation.Slight rejection occurred one month after operation with normal function of intestine but dysfunction of liver.In the first month after operation, abdominal infection was controlled by intraperitoneal drainage with open surgery.Immunosuppression protocol was administrated with alemtuzumab for induction plus maintenance treatment with tacrolimus, and mycophenolate mofetil was added because of renal dysfunction 2 years after transplantation.The patient was followed up for nearly 3 years with good quality of life without rejection and infection.Conclusion Combined liver and intestinal transplantation could improve patient's life quality and extend the survival time through the improvement of surgical techniques and individual immunosuppressive regimen.

Objective To study the imaging findings of the normal acromioclavicular joint and acromioclavicular dislocation.Methods CR films of normal shoulder in 68 cases and normal chest in 400 cases were collected.The distances of the acromioclavicular joint were measured,and the inferior cortex line of the acromioclavicular joint was observed on CR.MRI in 30 cases with normal shoulder,24 cases with acromioclavicular dislocation and 7 cases with shoulder impingement syndrome were also presented.Results The normal distance of the acromioclavicular joint was (3.36±0.44) mm.There was an arch line on the inferior cortex of the acromioclavicular joint for normal subjects.According to the Rockwood classification,acromioclavicular dislocation included type Ⅰ in 7/24 cases,type Ⅱ in 5/24 cases and type Ⅲ in 12 cases.The distances of the acromioclavicular joint were increased(＞4.3 mm) in type Ⅱ and type Ⅲ,and the inferior cortex lines of the acromioclavicular joint were not continual in type Ⅲ.MR imaging showed that the intra-articular fibrocartilaginous disk,the capsular and acromioclavicular ligament structure were ruptured in type Ⅱ,and coracoclavicular ligament torn in type Ⅲ.Conclusion The distance and the inferior cortex line of the acromioclavicular joint are of important value in diagnosis and classification of acromioclavicular dislocation.MRI is the most significant method in diagnosis of acromioclavicular dislocation.

Objective To discuss the surgical procedures and treatment after combined liver and intestinal transplantation with portal venous drainage and enterostomy of two ends in one case.Methods A male patient with liver dysfunction and short bowel syndrome underwent the combined liver and intestinal transplantation.With the techniques of en bloc,the liver and intestinal grafts were harvested from cadaveric donor.The intestinal graft,200 cm long,was implanted with portal venous drainage and aortic inflow,and enterostomy of both ends was performed instead of intestinal anastomosis.The liver graft was placed in a piggyback fashion with end to end anastomosis of the bile ducts without T tube. Inmunosuppression protocol was administrated with campath-1H and tacrolimus.Endoscopic biopsy of intestinal graft was performed regularly,and clinical observation was done to monitor the acute rejection.Results In the first month after operation,abdominal infection was controlled by intraperitoneal drainage with open surgery.One suspect acute rejection was treated with methylprednisolone.Until sixth month,the functions of liver and intestine were progressively restored.However,the patient lost weight and could not be free from intravenous nutrition because of diarrhea.Conclusion Combined liver and intestinal transplantation with portal venous drainage and enterostomy of two ends is a simple surgical procedure with lower risk of surgical complications.This method is propitious to monitoring rejection and function improvement of the grafts.Diarrhea and loss of digestive juice are the main reasons of low body weight and malnutrition.

Objective To evaluate the feasibility and value of dual-energy perfusion imaging (DEPI) of dual-source CT(DSCT) in the diagnosis of acute experimental pulmonary embolism. Methods Acute pulmonary embolism ( PE ) model was made in 8 New Zealand rabbits, and non-enhanced and enhanced DSCT scans were performed before and after embelization. Postprocessing of image data was made on the workstation, and CT pulmonary angiography ( CTPA ), DEal and fusion images were obtained. The location and number of the emboli were recorded. The rabbits were killed immediately after DSCT scan, the location and number of the lung lobes with pulmonary emboli were evaluated pathologically. Based on the pathological results, the sensitivity, specificity, positive predictive value ( PPV), and negative predictive value (NPV) of CTA, DEal and fusion images for the diagnosis of PE were calculated. Weighted Kappa values were calculated to evaluate the consistency between CTPA and DEal. Results PE model was made successfully in 7 rabbits. Six rabbits with 30 lobes were evaluated with one exception because of the catheter affecting the quality of lung peffusian image. PE was found pathologically in 18 lobar arteries. On DEPI, the region with PE showed low peffusion area comparing with the normal parenchyma and CTPA showed the filling defect within corresponding pulmonary artery or interruption of the artery. The sensitivity, specificity, PPVand NPV of CTPA were 66. 7% (12/18), 100.0% (12/12), 100.0% (12/12) and 66. 7% (12/18), respectively. The Kappa value was 0. 651 indicating moderate correlation with pathology. The sensitivity, specificity, aPv, and NPV of DEal were 88. 9% ( 16/18), 91.7% ( 11/12, 94. 1% (16/17) and 84. 6% ( 11/13 ), respectively. The Kappa value was 0. 795 indicating excellent correlation with pathology. Conclusion Dual-energy lung perfusion imaging of DSCT can display the blood distribution of rabbit's lung and has a high sensitivity for the diagnosis of acute pulmonary embolism.

ObjectiveAnalyzing the left atrium and pulmonary vein morphologicallv by 64 multislice spiral CT(MSCT)scan to guide the catheter ablation of Atrial fibrillation.MethodsTwo hundred and thirty-two patients(146 cases in atrial fibrillation group and 86 cases in control group)received 64 MSCT examination of the left atrium and pulmonary vein.The incidence of anatomical variation of pulmonary vein was compared between atrial fibrillation group and control group. For each group,the anatomical morphology ot every pulmonary vein and the auricle of left atrium was analyzed, the diameter of the orifice of each pulmonary vein and the size of left atrium were measured.ResultsSixty-four MSCT of left atrium and pulmonary vein could demonstrate detailed connecting type between left atrium and pulmonary Veins and the possible anatomieal variation. Anatomical variation of pulmonary vein in this study accounted for 16.8% (39/232)of total sample. For both groups,orifices of pulmonary veins appeared oval and their superoinferior diameters were larger than their anteroposterior diameters. There was significant difference in the inner diameter of left atrium between atrial fibrillation group and control group[atrial fibrillation group:(39.47±8.98)mm,control group:(36.94 ±5.49)mm,P=0.02],while there was no difference in the diameters of orifices ot puhnonary veins between two groups [ superoinferior diameters of pulmonary veins in atrial fibrillation group:left-up(18.15±1.35)mm,left-down(16.96 ±1.18)mm,right-up(17.50±

Objective To summarize the short-term results of simultaneous pancreas-kidney transplantation (SPK) at a single center in China.Methods SPK was performed on 12 consecutive patients from Jan.2010 to July 2014.All patients had long-standing insulin-dependent diabetes mellitus (IDDM) and subsequent renal failure.Bladder drainage (BD) of exocrine secretion was used in the 10 cases and enteric drainage (ED) in 2 patients.The patients were treated with quadruple therapy,which included ATG or anti-CD25 monoclonal antibody induction therapy,prednisone,tacrolimus and mycophenolat-mofetil (MMF).Results The SPK was performed successfully in 10 cases.One patient accepted re-pancreas transplantation due to necrotizing pancreatitis.One patient suffered hemorrhage of bladder,accepted 3 times of embolization therapy and died due to lung infection.Ten patients achieved excellent renal function and euglycemia,and no further insulin treatment was given in 9.5 ± 4.2 days posttransplant.Fasting plasma glucose returned to normal in 14.2 ± 5.1 days.Serum creatinine returned to normal in 10.4 ± 6.5 days.The mean hospital stay was 21.4 ± 7.3 days.One biopsy-proven renal rejection episodes occurred in 14 days postoperation.Main complications included wound infections on the side of pancreatic graft,lymphorrhagia,tacrolimus toxicity and urinary tract infection.Conclusion SPK is an effective therapy of ESRD.Donated graft protection system foundation,refinement and individualized treatment posttransplantion may be the key factors for successful SPK.

Objective To study the imaging finding of the coracoclavicular ligament.Methods 400 cases of normal chest films 200 men and 200 women were collected.The presented rates of pseudoarthrosis of the coracoclavicular joints and the clavicular tuberosities which are the coracoclavicular ligament attachment were evaluated and the distances between the clavicle and coracoid process interval were measured.There were 30 cases of normal shoulder MRI,the displaying rate of coracoclavicular ligament, the length and width of the coracoclavicular ligament were measured at MR imaging.8 case with type Ⅱ(n=5) and type Ⅲ(n=3)of acromioclavicular injury were also included in this study.Results Among 400 cases(800 sides),one pseudoarthrosis(0.25%)and 198 clavicular tuberosities(24.8%)were found.The normal distance between the clavicle and coracoid process interval was (6.92±3.16)mm.On MRI study,30 cases of coracoclavicular ligament were all showed on oblique coronal slices. The acromioclavicular ligament and coracoclavicular ligament tear were found in type Ⅱ and type Ⅲ,of acromioclavicular injury respectively on MRI.The length and width of conoid ligament were(11.48±1.43) mm and (4.82±1.21) mm respectively,and the length and width of trapezoid ligament were(9.09±0.84) mm and (5.10±0.87) mm respectively.Conclusion The normal anatomic measurement standards of the coracoclavicular ligament are established on X-ray and MRI,which is important for diagnosis of coracoclavicular ligament lesions.The coracoclavicular ligament torn is showed in typeⅢ of acromioclavicular dislocation.

Objective To investigate the effects of resveratrol (Res) on motor function and the anterior horn neuron of lumbar spinal cord after acute sciatic nerve compression injury in rats.Methods The rat model with acute sciatic nerve compression injury was established in 32 Sprague Dawley (SD) rats,which were randomly divided into four groups:Res group,Dimethyl Sulfoxide (DMSO) group,Normal Saline (NS) group and sham-operation group.Res,DMSO and saline were successively injected by intraperitoneal for 10 days after established crush acute sciatic nerve compression injury model,while sham-operation group was sutured only after exposure to the sciatic nerve.The weight,the change of toe extension angle,and the sciatic functional index (SFI) of rats were observed at the 1st day before operation and the 1st,3rd,7th,and 10th days after surgery.The expressions of neuron-specific nuclear protein (NeuN) and microtubule-associate protein 2 (MAP2) were detected by immunofluorescent staining of I4-L6 spinal cord anterior horn on the 10th day after surgery.Results No significant changes were found in the weight of rats among four groups.Compared to the sham,the motor function of the injured limb in Res,DMSO,and NS rats was impaired,and the anterior horn neurons were seriously damaged.But the differences of the change of toe extension and the sciatic functional index of rats were significantly higher in Res group than that of the DMSO group and NS group (P ＜ 0.01) at the 3rd,7th,and 10th days after surgery.The expressions of NeuN and MAP2 in the anterior horn of rat lumbar spinal cord were up-regulated in Res group relative to DMSO and NS,and the number of neurons in the lumbar spinal cord was significantly relieved at the 10th days.Conclusions Res was significant to rat model of acute sciatic nerve injury,which could increase the number of neurons in the anterior horn of the spinal cord and help the recovery of motor function.